Click here to switch to the map view.

The map label for this gene is purF [H]

Identifier: 188585773

GI number: 188585773

Start: 1212199

End: 1213635

Strand: Reverse

Name: purF [H]

Synonym: Nther_1147

Alternate gene names: 188585773

Gene position: 1213635-1212199 (Counterclockwise)

Preceding gene: 188585777

Following gene: 188585767

Centisome position: 38.34

GC content: 36.33

Gene sequence:

>1437_bases
ATGCTAATTTATGAGTCCTTTAATCAACAATTTATATATATATTAACGAAGGGAGGAATTGAAGTGGAAGAGGAATGCGG
AATTTTTGGAATATATGCCCCAGATCAAGACGTAGCCCAATTAACTTATTACGGGTTATATGCTCTACAACATCGTGGTC
AAGAAAGTACCGGAATTTCTGTTTCCAATTCAAATAAGTTAGTAACTAATAAAAATATGGGATTAGTTAATGAGGTTTTC
GATGAGCACAATTTATCTGAACTAACTGGCATCAGCGCCATAGGTCATGTTCGCTATACAACAGAGGGAGACAGTTCGGT
TGTTAATGCTCAACCCTTAACTGTAAAATGCAAATTAGGTGAGCTATCTGTGGCTCATAATGGCAATTTAATAAATTCCG
AAGAACTTAGAAATCGACTAGAAAAAGAAGGTACTATTTTCCATACCAATAGTGACAGTGAAATCCTGGCACATCTTTTG
GCGAAATCTCAAGAAAATGATTTATTAGCAGCTTTTCAAGAAGTTATAAAATCCATTCAAGGAGCTTATAACTTTTTAAT
GTTAACCCCAGACAAAATATTAGCAGTACGTGATCCCTGGGGATTTAGACCTCTATCTCTGGGAAAGGTCGCGGGAAACT
ACGTAGTGGCTTCGGAAACTTGTGCCTTTGATACTATTGGAGCAGAATTTCTCCGCGATATAGAACCAGGTGAAATGGTC
TGTATTGATCACAATGGTTTAAATTCTTATCAGGTTTTTGAGAAAACAAAACCTTCACTGTGCATGTTTGAATATATTTA
TTTTGCAAGACCTGATAGCAACATAAATAATCGCAATGTTCACCTAGTCCGAAAAGAGCTAGGGCGCGAATTAGCTAAGG
AATTACCAGAGGAAATCCCAGCTAAAGCAGATCTGGTTTCTGGTGTTCCTGATTCAAGTCTTTCAGCAGCATCAGGAGTT
TCCGAAGAATTGCCAGCACCTTACGAAATGGCTCTGATAAAAAATCGCTATGTAGGAAGAACTTTTATCAAACCTAATCA
AACTAATCGAGAAATCAGCGTAAAAATTAAATTAAACCCTGTTGAGAGAACAGTAGCAAATAAAAATTTAATTTTAGTTG
ATGATTCTATTGTCCGTGGTACTACCATTTCCAACATAATAAAAACACTTAAAAAATCCGGCACAAGTCAAATTCATGTC
TTGGTCAGTTCTCCTCCAGTTAAATATCCTTGTTGTTTCGGTATAGATACTTCCACCAGTAGTGAACTGATTGCCAGTAA
TCAATCCGTTTCTAATATAAAAGATCATATTCAAGCTGATAGTCTTCAATATTTGAGTGTTGAAGGTATGCTTCGAGCGG
TTAACAATGTCAGTAAGATTCAAAATAATGAAGGTTTCTGTTTAGCTTGTTTTAACGGTGAAAACCCGAGCTTGTAA

Upstream 100 bases:

>100_bases
ACTAAAAATCGTTTTCCTGGTATATTTTCGTATTATCTCCGAATTTTATTTCTTATTTTGTATTATTGTTTATTTTAATT
GTGTTGAAACAACTCAGGAA

Downstream 100 bases:

>100_bases
ACATTTCAAATTATATTAGTGGAAAGAACAAAAACCAGGCGCCGGTAGCCACAGAACTTAGGCGCCTGGTTTTTTTATAA
CTCCTGAATTGTCACCTTGT

Product: amidophosphoribosyltransferase

Products: NA

Alternate protein names: ATase; Glutamine phosphoribosylpyrophosphate amidotransferase; GPATase [H]

Number of amino acids: Translated: 478; Mature: 478

Protein sequence:

>478_residues
MLIYESFNQQFIYILTKGGIEVEEECGIFGIYAPDQDVAQLTYYGLYALQHRGQESTGISVSNSNKLVTNKNMGLVNEVF
DEHNLSELTGISAIGHVRYTTEGDSSVVNAQPLTVKCKLGELSVAHNGNLINSEELRNRLEKEGTIFHTNSDSEILAHLL
AKSQENDLLAAFQEVIKSIQGAYNFLMLTPDKILAVRDPWGFRPLSLGKVAGNYVVASETCAFDTIGAEFLRDIEPGEMV
CIDHNGLNSYQVFEKTKPSLCMFEYIYFARPDSNINNRNVHLVRKELGRELAKELPEEIPAKADLVSGVPDSSLSAASGV
SEELPAPYEMALIKNRYVGRTFIKPNQTNREISVKIKLNPVERTVANKNLILVDDSIVRGTTISNIIKTLKKSGTSQIHV
LVSSPPVKYPCCFGIDTSTSSELIASNQSVSNIKDHIQADSLQYLSVEGMLRAVNNVSKIQNNEGFCLACFNGENPSL

Sequences:

>Translated_478_residues
MLIYESFNQQFIYILTKGGIEVEEECGIFGIYAPDQDVAQLTYYGLYALQHRGQESTGISVSNSNKLVTNKNMGLVNEVF
DEHNLSELTGISAIGHVRYTTEGDSSVVNAQPLTVKCKLGELSVAHNGNLINSEELRNRLEKEGTIFHTNSDSEILAHLL
AKSQENDLLAAFQEVIKSIQGAYNFLMLTPDKILAVRDPWGFRPLSLGKVAGNYVVASETCAFDTIGAEFLRDIEPGEMV
CIDHNGLNSYQVFEKTKPSLCMFEYIYFARPDSNINNRNVHLVRKELGRELAKELPEEIPAKADLVSGVPDSSLSAASGV
SEELPAPYEMALIKNRYVGRTFIKPNQTNREISVKIKLNPVERTVANKNLILVDDSIVRGTTISNIIKTLKKSGTSQIHV
LVSSPPVKYPCCFGIDTSTSSELIASNQSVSNIKDHIQADSLQYLSVEGMLRAVNNVSKIQNNEGFCLACFNGENPSL
>Mature_478_residues
MLIYESFNQQFIYILTKGGIEVEEECGIFGIYAPDQDVAQLTYYGLYALQHRGQESTGISVSNSNKLVTNKNMGLVNEVF
DEHNLSELTGISAIGHVRYTTEGDSSVVNAQPLTVKCKLGELSVAHNGNLINSEELRNRLEKEGTIFHTNSDSEILAHLL
AKSQENDLLAAFQEVIKSIQGAYNFLMLTPDKILAVRDPWGFRPLSLGKVAGNYVVASETCAFDTIGAEFLRDIEPGEMV
CIDHNGLNSYQVFEKTKPSLCMFEYIYFARPDSNINNRNVHLVRKELGRELAKELPEEIPAKADLVSGVPDSSLSAASGV
SEELPAPYEMALIKNRYVGRTFIKPNQTNREISVKIKLNPVERTVANKNLILVDDSIVRGTTISNIIKTLKKSGTSQIHV
LVSSPPVKYPCCFGIDTSTSSELIASNQSVSNIKDHIQADSLQYLSVEGMLRAVNNVSKIQNNEGFCLACFNGENPSL

Specific function: De novo purine biosynthesis; first step. [C]

COG id: COG0034

COG function: function code F; Glutamine phosphoribosylpyrophosphate amidotransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI29570798, Length=509, Percent_Identity=40.0785854616896, Blast_Score=351, Evalue=1e-96,
Organism=Homo sapiens, GI4826742, Length=235, Percent_Identity=24.6808510638298, Blast_Score=67, Evalue=3e-11,
Organism=Escherichia coli, GI1788651, Length=467, Percent_Identity=38.7580299785867, Blast_Score=297, Evalue=1e-81,
Organism=Escherichia coli, GI1790167, Length=240, Percent_Identity=31.6666666666667, Blast_Score=99, Evalue=8e-22,
Organism=Caenorhabditis elegans, GI17554892, Length=479, Percent_Identity=37.7870563674322, Blast_Score=321, Evalue=5e-88,
Organism=Saccharomyces cerevisiae, GI6323958, Length=450, Percent_Identity=34.6666666666667, Blast_Score=253, Evalue=4e-68,
Organism=Drosophila melanogaster, GI24659598, Length=487, Percent_Identity=40.8624229979466, Blast_Score=370, Evalue=1e-102,
Organism=Drosophila melanogaster, GI28573187, Length=489, Percent_Identity=39.8773006134969, Blast_Score=358, Evalue=4e-99,
Organism=Drosophila melanogaster, GI24659604, Length=462, Percent_Identity=41.1255411255411, Blast_Score=357, Evalue=1e-98,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005854
- InterPro:   IPR000583
- InterPro:   IPR017932
- InterPro:   IPR000836 [H]

Pfam domain/function: PF00310 GATase_2; PF00156 Pribosyltran [H]

EC number: =2.4.2.14 [H]

Molecular weight: Translated: 52722; Mature: 52722

Theoretical pI: Translated: 5.12; Mature: 5.12

Prosite motif: PS00103 PUR_PYR_PR_TRANSFER

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIYESFNQQFIYILTKGGIEVEEECGIFGIYAPDQDVAQLTYYGLYALQHRGQESTGIS
CEEEECCCCEEEEEEECCCCEEHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEE
VSNSNKLVTNKNMGLVNEVFDEHNLSELTGISAIGHVRYTTEGDSSVVNAQPLTVKCKLG
ECCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHCCEEEEECCCCCCEECCCCEEEEEECC
ELSVAHNGNLINSEELRNRLEKEGTIFHTNSDSEILAHLLAKSQENDLLAAFQEVIKSIQ
CEEEECCCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GAYNFLMLTPDKILAVRDPWGFRPLSLGKVAGNYVVASETCAFDTIGAEFLRDIEPGEMV
CCEEEEEECCCCEEEEECCCCCCCCCHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCEE
CIDHNGLNSYQVFEKTKPSLCMFEYIYFARPDSNINNRNVHLVRKELGRELAKELPEEIP
EEECCCCCCCHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
AKADLVSGVPDSSLSAASGVSEELPAPYEMALIKNRYVGRTFIKPNQTNREISVKIKLNP
CCHHHHCCCCCCCCHHHCCCHHHCCCCHHHHHHHCCCCCEEEECCCCCCCEEEEEEEECH
VERTVANKNLILVDDSIVRGTTISNIIKTLKKSGTSQIHVLVSSPPVKYPCCFGIDTSTS
HHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCC
SELIASNQSVSNIKDHIQADSLQYLSVEGMLRAVNNVSKIQNNEGFCLACFNGENPSL
CHHHHCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC
>Mature Secondary Structure
MLIYESFNQQFIYILTKGGIEVEEECGIFGIYAPDQDVAQLTYYGLYALQHRGQESTGIS
CEEEECCCCEEEEEEECCCCEEHHCCCEEEEECCCCHHHHHHHHHHHHHHHCCCCCCCEE
VSNSNKLVTNKNMGLVNEVFDEHNLSELTGISAIGHVRYTTEGDSSVVNAQPLTVKCKLG
ECCCCEEEEECCCCHHHHHHCCCCHHHHHHHHHCCEEEEECCCCCCEECCCCEEEEEECC
ELSVAHNGNLINSEELRNRLEKEGTIFHTNSDSEILAHLLAKSQENDLLAAFQEVIKSIQ
CEEEECCCCCCCHHHHHHHHHHCCEEEEECCCHHHHHHHHHCCCCCHHHHHHHHHHHHHH
GAYNFLMLTPDKILAVRDPWGFRPLSLGKVAGNYVVASETCAFDTIGAEFLRDIEPGEMV
CCEEEEEECCCCEEEEECCCCCCCCCHHHCCCCEEEEECCCCHHHHHHHHHHCCCCCCEE
CIDHNGLNSYQVFEKTKPSLCMFEYIYFARPDSNINNRNVHLVRKELGRELAKELPEEIP
EEECCCCCCCHHHHCCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHHHHHHHHHHHHCC
AKADLVSGVPDSSLSAASGVSEELPAPYEMALIKNRYVGRTFIKPNQTNREISVKIKLNP
CCHHHHCCCCCCCCHHHCCCHHHCCCCHHHHHHHCCCCCEEEECCCCCCCEEEEEEEECH
VERTVANKNLILVDDSIVRGTTISNIIKTLKKSGTSQIHVLVSSPPVKYPCCFGIDTSTS
HHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHCCCCEEEEEECCCCCCCCEEECCCCCCC
SELIASNQSVSNIKDHIQADSLQYLSVEGMLRAVNNVSKIQNNEGFCLACFNGENPSL
CHHHHCCCCHHHHHHHHCCCCEEEEEHHHHHHHHHHHHHHCCCCCEEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 6411717; 3036807; 9384377; 6411716; 6094545; 3090047; 8197456; 9271502 [H]