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The map label for this gene is iadA [H]

Identifier: 188585631

GI number: 188585631

Start: 1067583

End: 1068764

Strand: Reverse

Name: iadA [H]

Synonym: Nther_1004

Alternate gene names: 188585631

Gene position: 1068764-1067583 (Counterclockwise)

Preceding gene: 188585632

Following gene: 188585629

Centisome position: 33.76

GC content: 38.24

Gene sequence:

>1182_bases
ATGGTTACTTTAATACGCGGAGGACAGCTTTACACACCTGAGTATCAAGGTAAAAAAGATGTCCTGTTAACAGGTGAAAA
AATTGCTTACATCGATGACAATATCAACTTACAATCTCCTTCCTCACAAGATGGAATTGTAGAAGAAATTGATGCTCGCG
GCAAATTTGTTGTGCCAGGTTTTATAGATGGTCATGTCCATCTCATTGGTGGTGGTGGCGAAGGTGGCTTCGCCACCCGA
ACCCCTGAAATTGAACTTTCTAATATTATAACTTCTGGAATTACCACTGTTATTGGATGTCTCGGTACTGACGGTATCAC
CCGAGAAATGAAAGACCTCCTAGCAAAAGCACGTGGACTAAGAGAAGAAGGAATTTCTACATATATCTTGTCCGGATCAT
ATAAAGTGCCTGTAACAACCTTAACCGGTGAAGTACAAAAGGATATTGTTTTAGTTCCAGAAGTTCTTGGTGTAGGAGAA
ATAGCTTTATCAGATCATAGATCCTTCGAACCAACAATATCCGAATTGAAAAAATTGGTTGCAGAAACCAGAGTAGCAGC
TATGTTATCCGGTAAAAAAGGTATCATAAACGTACACATGGGCGATGGAGTTAAAATGTTCCAAGATATTGACAGAATAA
TTGAAGAAACTGAGCTTCCCGGTTCTCACTTTGTTGTGACTCATGGCAATCGAACCCAAGGCTTATTCAATGAAGGATTA
GAATTAACCCAAAAAGGAGTCTTTTTAGACTTAACGACTAGTACAGTACAGAAATTCATAGAAGACGGTGAAATCAAGTG
TTCCCGAGCGCTAAAATATTTTGATGATAATGGGATAACGCCAGACAACGTAACGTTTTCTTCTGACGGTCAAGGTAGTC
TTCCGAATTTTAGTGAGTCTGGAGATTATTTAGGCTTAAAAGTCGGAGGTGTAGAATCACTATATAGAGAAGTTAAAGAA
GCTGTCACTGAGGAAAATGTACCCTTAGAAAATGCTCTCAGGGTCATCACAAAAAATCCAGCTTCTGCATACGGACTAAA
CAACAAAGGAGAACTAACAAAAGGTAAAGATGGTGACTTAATACTACTCGATGAAGATACTTTAGAAATCGATACAGTTA
TCTCCCGAGGAAAAACATTAATTAAAAATAAAGAAATTTTGCAAAAAGGTACCTTCGAATAA

Upstream 100 bases:

>100_bases
TAATAAATGCATTTAATTGATTATAAAACCAAATAAACGAAATTAGTTTGTGAATATCAAAACTCTAAATTTATAAGAAC
TAATTGAAAGGGGTTAATTG

Downstream 100 bases:

>100_bases
TAGAGTCCTGAGTCCTCCAAAAGCACGTGGACTAAGAGAAGAAGTGAGAACCTCCCAGAAAGTTTATTGAATCTCTGGGA
GGTTCATTTATATTGCTAGA

Product: isoaspartyl dipeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 393; Mature: 393

Protein sequence:

>393_residues
MVTLIRGGQLYTPEYQGKKDVLLTGEKIAYIDDNINLQSPSSQDGIVEEIDARGKFVVPGFIDGHVHLIGGGGEGGFATR
TPEIELSNIITSGITTVIGCLGTDGITREMKDLLAKARGLREEGISTYILSGSYKVPVTTLTGEVQKDIVLVPEVLGVGE
IALSDHRSFEPTISELKKLVAETRVAAMLSGKKGIINVHMGDGVKMFQDIDRIIEETELPGSHFVVTHGNRTQGLFNEGL
ELTQKGVFLDLTTSTVQKFIEDGEIKCSRALKYFDDNGITPDNVTFSSDGQGSLPNFSESGDYLGLKVGGVESLYREVKE
AVTEENVPLENALRVITKNPASAYGLNNKGELTKGKDGDLILLDEDTLEIDTVISRGKTLIKNKEILQKGTFE

Sequences:

>Translated_393_residues
MVTLIRGGQLYTPEYQGKKDVLLTGEKIAYIDDNINLQSPSSQDGIVEEIDARGKFVVPGFIDGHVHLIGGGGEGGFATR
TPEIELSNIITSGITTVIGCLGTDGITREMKDLLAKARGLREEGISTYILSGSYKVPVTTLTGEVQKDIVLVPEVLGVGE
IALSDHRSFEPTISELKKLVAETRVAAMLSGKKGIINVHMGDGVKMFQDIDRIIEETELPGSHFVVTHGNRTQGLFNEGL
ELTQKGVFLDLTTSTVQKFIEDGEIKCSRALKYFDDNGITPDNVTFSSDGQGSLPNFSESGDYLGLKVGGVESLYREVKE
AVTEENVPLENALRVITKNPASAYGLNNKGELTKGKDGDLILLDEDTLEIDTVISRGKTLIKNKEILQKGTFE
>Mature_393_residues
MVTLIRGGQLYTPEYQGKKDVLLTGEKIAYIDDNINLQSPSSQDGIVEEIDARGKFVVPGFIDGHVHLIGGGGEGGFATR
TPEIELSNIITSGITTVIGCLGTDGITREMKDLLAKARGLREEGISTYILSGSYKVPVTTLTGEVQKDIVLVPEVLGVGE
IALSDHRSFEPTISELKKLVAETRVAAMLSGKKGIINVHMGDGVKMFQDIDRIIEETELPGSHFVVTHGNRTQGLFNEGL
ELTQKGVFLDLTTSTVQKFIEDGEIKCSRALKYFDDNGITPDNVTFSSDGQGSLPNFSESGDYLGLKVGGVESLYREVKE
AVTEENVPLENALRVITKNPASAYGLNNKGELTKGKDGDLILLDEDTLEIDTVISRGKTLIKNKEILQKGTFE

Specific function: Catalyzes the hydrolytic cleavage of a subset of L- isoaspartyl (L-beta-aspartyl) dipeptides. Used to degrade proteins damaged by L-isoaspartyl residues formation. The best substrate for the enzyme reported thus far is iso-Asp-Leu [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M38 family [H]

Homologues:

Organism=Escherichia coli, GI1790784, Length=391, Percent_Identity=41.1764705882353, Blast_Score=288, Evalue=3e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011059
- InterPro:   IPR010229 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.4.19.- [C]

Molecular weight: Translated: 42565; Mature: 42565

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTLIRGGQLYTPEYQGKKDVLLTGEKIAYIDDNINLQSPSSQDGIVEEIDARGKFVVPG
CEEEEECCEEECCCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCCHHHHCCCCCEEECE
FIDGHVHLIGGGGEGGFATRTPEIELSNIITSGITTVIGCLGTDGITREMKDLLAKARGL
EECCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
REEGISTYILSGSYKVPVTTLTGEVQKDIVLVPEVLGVGEIALSDHRSFEPTISELKKLV
HHCCCEEEEECCCEEEEEEEECCCCCCCEEEECHHHCCCCEEECCCCCCCHHHHHHHHHH
AETRVAAMLSGKKGIINVHMGDGVKMFQDIDRIIEETELPGSHFVVTHGNRTQGLFNEGL
HHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCH
ELTQKGVFLDLTTSTVQKFIEDGEIKCSRALKYFDDNGITPDNVTFSSDGQGSLPNFSES
HHHHCCEEEEECHHHHHHHHHCCCCEEHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCC
GDYLGLKVGGVESLYREVKEAVTEENVPLENALRVITKNPASAYGLNNKGELTKGKDGDL
CCEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCE
ILLDEDTLEIDTVISRGKTLIKNKEILQKGTFE
EEEECCCCHHHHHHHCCHHHHHCHHHHHCCCCC
>Mature Secondary Structure
MVTLIRGGQLYTPEYQGKKDVLLTGEKIAYIDDNINLQSPSSQDGIVEEIDARGKFVVPG
CEEEEECCEEECCCCCCCCCEEEECCEEEEEECCCCCCCCCCCCCCHHHHCCCCCEEECE
FIDGHVHLIGGGGEGGFATRTPEIELSNIITSGITTVIGCLGTDGITREMKDLLAKARGL
EECCEEEEEECCCCCCCCCCCCCEEHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHCC
REEGISTYILSGSYKVPVTTLTGEVQKDIVLVPEVLGVGEIALSDHRSFEPTISELKKLV
HHCCCEEEEECCCEEEEEEEECCCCCCCEEEECHHHCCCCEEECCCCCCCHHHHHHHHHH
AETRVAAMLSGKKGIINVHMGDGVKMFQDIDRIIEETELPGSHFVVTHGNRTQGLFNEGL
HHHHHHHHHCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCCEEEEECCCCCCHHHHHCH
ELTQKGVFLDLTTSTVQKFIEDGEIKCSRALKYFDDNGITPDNVTFSSDGQGSLPNFSES
HHHHCCEEEEECHHHHHHHHHCCCCEEHHHHHHHCCCCCCCCCEEECCCCCCCCCCCCCC
GDYLGLKVGGVESLYREVKEAVTEENVPLENALRVITKNPASAYGLNNKGELTKGKDGDL
CCEEEEEECCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCHHCCCCCCCCCCCCCCCCE
ILLDEDTLEIDTVISRGKTLIKNKEILQKGTFE
EEEECCCCHHHHHHHCCHHHHHCHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7876157; 7610040; 9278503 [H]