Click here to switch to the map view.

The map label for this gene is glnA [H]

Identifier: 188585589

GI number: 188585589

Start: 1021321

End: 1022658

Strand: Direct

Name: glnA [H]

Synonym: Nther_0962

Alternate gene names: 188585589

Gene position: 1021321-1022658 (Clockwise)

Preceding gene: 188585588

Following gene: 188585590

Centisome position: 32.26

GC content: 36.47

Gene sequence:

>1338_bases
ATGTGTAAATTTACTAAAGAAGATGTTTTGAAAATGGCAAAAGAGCAAAACGTGAAATTTATTAGGTTGCAATTTACTGA
CATTTTTGGAATTATGAAAAATGTTGCAATTCCAGTAAGTCAGCTAGAAAAAGCTTTGGATGGGGAAATGATGTTTGATG
GATCCTCAGTTGAAGGTTTTGTTAGAATCGAAGAAAGCGATATGTATTTACAACCAGATCCGAATACATTTATAACTTTC
CCTTGGAGATCAGGAATTGATGGGGATGTAGGAAGGTTGATTTGTGATGTTTATGATTCTACAGAAGGGAAGCCATTTGC
AGGTTGCCCTAGAAGTGTGCTTAGAAGTTTGGTTGATAAGGCAGTCCAAAAGGGGTATACTTTGAACGCAGGTCCAGAAG
CTGAATTTTTCTTATTTTTAACTGATAGTGAAGGTCGACCAACTACCATCACTCATGATAAAGCCGGATATTTTGATTTA
TCACCTGTAGACCTGGGTGAAGGTGCAAGAAGAGACATGGTTACTACTCTTCAAAAAATGGGATATGAGATTGAAGCATC
TCATCACGAAGTGGCACCAGGACAGCACGAAATTGACTTTCAGTATTCAGATGCTCTGGATATTGCTGATAAAATAGTCA
CATTTAAATTGGTTGTAAGAAGTATAGCACAACGACATGGCCTTCATGCTACTTTTATGCCGAAACCAATTCAGGGTATA
AATGGTTCAGGTATGCATATTAATTTATCATTGTTTAAAAATGGAGGGAACACTTTTTATGATCCACAAAAAGAAAATGG
TTTAAGTGACGTAGCCGTTAATTTTGTTGGTGGTCTGCTAAAGCACGGAAAGGCCATTACTGCAATTACAAATCCAACTG
TCAATTCCTATAAAAGATTAGTGCCTGGCTATGAAGCACCGGTTAATATGGCATGGTCATTTAGAAACAGAAGTCCGCTA
ATTAGAATTCCTGGTAAGAGGGGTTCGTCAACAAGAACTGAGTTACGAAGCCCTGATCCTTCTTTTAATCCTTACTTATC
TTTAGCAGCGATTTTAGCAGCTGGTTTAGACGGTGTTGAAAATCAAATCGATCCCCCTGCACCTGTTGATAGAAATATTT
ATGATATGACTGAAGAAAGCTTAGAAGAACTACAAATTGATCAATTACCAGAAACTTTAGAAAAAGCAATTAATCATTTA
GAGGAAGATGAATTGATTAAAAATGCTTTAGGTGAACATGTTTGCGAGAAATTCATTGAAGCCAAAAAAATTGAATGGAA
AAATTACATGTCAGCAGTTCACCAGTGGGAGCTAGACGAATATTTAACAAAATTTTAG

Upstream 100 bases:

>100_bases
GTGAGCAAAGGCGTTCACTTGTACAGTTGTATTATATTAATACGACTGTACTAGGGACGCCTTAAATTATTTTATAGAAA
TTATCAGGAGGTATATGAAC

Downstream 100 bases:

>100_bases
TATATAAATTAGAGTCTATTTCATGAAATCTTAGGGATGGAGTGGTTACAGTGCCTGAGGCGAATGTAGTAGTTGCCTCC
GGAGAAACTGAGTATAGGAA

Product: L-glutamine synthetase

Products: NA

Alternate protein names: Glutamate--ammonia ligase [H]

Number of amino acids: Translated: 445; Mature: 445

Protein sequence:

>445_residues
MCKFTKEDVLKMAKEQNVKFIRLQFTDIFGIMKNVAIPVSQLEKALDGEMMFDGSSVEGFVRIEESDMYLQPDPNTFITF
PWRSGIDGDVGRLICDVYDSTEGKPFAGCPRSVLRSLVDKAVQKGYTLNAGPEAEFFLFLTDSEGRPTTITHDKAGYFDL
SPVDLGEGARRDMVTTLQKMGYEIEASHHEVAPGQHEIDFQYSDALDIADKIVTFKLVVRSIAQRHGLHATFMPKPIQGI
NGSGMHINLSLFKNGGNTFYDPQKENGLSDVAVNFVGGLLKHGKAITAITNPTVNSYKRLVPGYEAPVNMAWSFRNRSPL
IRIPGKRGSSTRTELRSPDPSFNPYLSLAAILAAGLDGVENQIDPPAPVDRNIYDMTEESLEELQIDQLPETLEKAINHL
EEDELIKNALGEHVCEKFIEAKKIEWKNYMSAVHQWELDEYLTKF

Sequences:

>Translated_445_residues
MCKFTKEDVLKMAKEQNVKFIRLQFTDIFGIMKNVAIPVSQLEKALDGEMMFDGSSVEGFVRIEESDMYLQPDPNTFITF
PWRSGIDGDVGRLICDVYDSTEGKPFAGCPRSVLRSLVDKAVQKGYTLNAGPEAEFFLFLTDSEGRPTTITHDKAGYFDL
SPVDLGEGARRDMVTTLQKMGYEIEASHHEVAPGQHEIDFQYSDALDIADKIVTFKLVVRSIAQRHGLHATFMPKPIQGI
NGSGMHINLSLFKNGGNTFYDPQKENGLSDVAVNFVGGLLKHGKAITAITNPTVNSYKRLVPGYEAPVNMAWSFRNRSPL
IRIPGKRGSSTRTELRSPDPSFNPYLSLAAILAAGLDGVENQIDPPAPVDRNIYDMTEESLEELQIDQLPETLEKAINHL
EEDELIKNALGEHVCEKFIEAKKIEWKNYMSAVHQWELDEYLTKF
>Mature_445_residues
MCKFTKEDVLKMAKEQNVKFIRLQFTDIFGIMKNVAIPVSQLEKALDGEMMFDGSSVEGFVRIEESDMYLQPDPNTFITF
PWRSGIDGDVGRLICDVYDSTEGKPFAGCPRSVLRSLVDKAVQKGYTLNAGPEAEFFLFLTDSEGRPTTITHDKAGYFDL
SPVDLGEGARRDMVTTLQKMGYEIEASHHEVAPGQHEIDFQYSDALDIADKIVTFKLVVRSIAQRHGLHATFMPKPIQGI
NGSGMHINLSLFKNGGNTFYDPQKENGLSDVAVNFVGGLLKHGKAITAITNPTVNSYKRLVPGYEAPVNMAWSFRNRSPL
IRIPGKRGSSTRTELRSPDPSFNPYLSLAAILAAGLDGVENQIDPPAPVDRNIYDMTEESLEELQIDQLPETLEKAINHL
EEDELIKNALGEHVCEKFIEAKKIEWKNYMSAVHQWELDEYLTKF

Specific function: Unknown

COG id: COG0174

COG function: function code E; Glutamine synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glutamine synthetase family [H]

Homologues:

Organism=Homo sapiens, GI221307485, Length=451, Percent_Identity=26.6075388026608, Blast_Score=129, Evalue=5e-30,
Organism=Escherichia coli, GI1790301, Length=475, Percent_Identity=40, Blast_Score=298, Evalue=5e-82,
Organism=Escherichia coli, GI87081870, Length=460, Percent_Identity=27.3913043478261, Blast_Score=146, Evalue=3e-36,

Paralogues:

None

Copy number: 420 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 1040 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 15324 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 8,000 Molecules/Cell In: Glucose minimal medi

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008147
- InterPro:   IPR014746
- InterPro:   IPR008146
- InterPro:   IPR004809 [H]

Pfam domain/function: PF00120 Gln-synt_C; PF03951 Gln-synt_N [H]

EC number: =6.3.1.2 [H]

Molecular weight: Translated: 49848; Mature: 49848

Theoretical pI: Translated: 4.92; Mature: 4.92

Prosite motif: PS00180 GLNA_1 ; PS00181 GLNA_ATP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MCKFTKEDVLKMAKEQNVKFIRLQFTDIFGIMKNVAIPVSQLEKALDGEMMFDGSSVEGF
CCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHCCCEEECCCCCCEE
VRIEESDMYLQPDPNTFITFPWRSGIDGDVGRLICDVYDSTEGKPFAGCPRSVLRSLVDK
EEEECCCEEECCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
AVQKGYTLNAGPEAEFFLFLTDSEGRPTTITHDKAGYFDLSPVDLGEGARRDMVTTLQKM
HHHCCEEECCCCCCEEEEEEECCCCCCCEEECCCCCEEECCCCCCCCCCHHHHHHHHHHC
GYEIEASHHEVAPGQHEIDFQYSDALDIADKIVTFKLVVRSIAQRHGLHATFMPKPIQGI
CCEEECCCCCCCCCCCEECEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
NGSGMHINLSLFKNGGNTFYDPQKENGLSDVAVNFVGGLLKHGKAITAITNPTVNSYKRL
CCCCEEEEEEEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
VPGYEAPVNMAWSFRNRSPLIRIPGKRGSSTRTELRSPDPSFNPYLSLAAILAAGLDGVE
CCCCCCCCCEEEEECCCCCEEEECCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCHH
NQIDPPAPVDRNIYDMTEESLEELQIDQLPETLEKAINHLEEDELIKNALGEHVCEKFIE
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AKKIEWKNYMSAVHQWELDEYLTKF
HHHCHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MCKFTKEDVLKMAKEQNVKFIRLQFTDIFGIMKNVAIPVSQLEKALDGEMMFDGSSVEGF
CCCCCHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHCCCHHHHHHHHCCCEEECCCCCCEE
VRIEESDMYLQPDPNTFITFPWRSGIDGDVGRLICDVYDSTEGKPFAGCPRSVLRSLVDK
EEEECCCEEECCCCCCEEEECCCCCCCCCHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHH
AVQKGYTLNAGPEAEFFLFLTDSEGRPTTITHDKAGYFDLSPVDLGEGARRDMVTTLQKM
HHHCCEEECCCCCCEEEEEEECCCCCCCEEECCCCCEEECCCCCCCCCCHHHHHHHHHHC
GYEIEASHHEVAPGQHEIDFQYSDALDIADKIVTFKLVVRSIAQRHGLHATFMPKPIQGI
CCEEECCCCCCCCCCCEECEEECCHHHHHHHHHHHHHHHHHHHHHCCCEEEECCCCCCCC
NGSGMHINLSLFKNGGNTFYDPQKENGLSDVAVNFVGGLLKHGKAITAITNPTVNSYKRL
CCCCEEEEEEEEECCCCCCCCCCHHCCHHHHHHHHHHHHHHCCCEEEEECCCCHHHHHHH
VPGYEAPVNMAWSFRNRSPLIRIPGKRGSSTRTELRSPDPSFNPYLSLAAILAAGLDGVE
CCCCCCCCCEEEEECCCCCEEEECCCCCCCCHHHCCCCCCCCCHHHHHHHHHHHCCCCHH
NQIDPPAPVDRNIYDMTEESLEELQIDQLPETLEKAINHLEEDELIKNALGEHVCEKFIE
HCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHH
AKKIEWKNYMSAVHQWELDEYLTKF
HHHCHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2891680; 1981087 [H]