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The map label for this gene is cobD [H]

Identifier: 188585568

GI number: 188585568

Start: 996648

End: 997622

Strand: Direct

Name: cobD [H]

Synonym: Nther_0940

Alternate gene names: 188585568

Gene position: 996648-997622 (Clockwise)

Preceding gene: 188585567

Following gene: 188585569

Centisome position: 31.48

GC content: 36.41

Gene sequence:

>975_bases
ATGGAAATAATCGGAACTTCAATATTGATAGTTATATTTGCTTTTTTAATTGACAGCCTCTTGGGAGATTTTCTGGGCCG
TTATCACCCTGTTTGTTTAATTGCCCTGTTAATTAATTTTTTATGGGAAAAGATTGTAACAATCAGTACTTCTTGGGGCT
TTAATTTTCTTTTGGAAGGAAACAACTTTTCTGGTCTAGCTAAATTCGCCGGATTTTTAATAACGATTATAGTAGTTGGT
GTGACTTTTACTTTAAGTTATTACTTAGCTTCCATGCATTTCATAATAGATATTTTATTAATATATTTTGCGTTATCTTA
TAAAAGTTTAGTGGAGCATCTGGCTCGTGTTTTATGTCCTTTATCACATGGTGATTTAATCAAAGCCAGGGAAGAATTGT
CGAAAATCGTTGGTAGGGACGTGGACTATCTGGAGGAAGCTGAAATTGCCAGGGGTGGTATAGAAACTAGTGCAGAAAGT
TTATGTGACGGAATTATAGCACCTTTATTTTTTGCTTTTATTGGTGGGGCCCCCCTGGTAATGTCCTATAAAGCAGTCAA
CACTTTGGATTCAATGATCGGTTACAACTTTTATCCCTACAAAGATCTTGGCTTTGCCTCGGCCAAGTTGGATGATGTAT
TAAATCTGATACCGGCTCGTCTAACTGCCGTTTTTATCTTAATTACATCATTACTAACAAAGAAAAACTTTGTTAAAGGC
CTGCAAGTTATGAGACATGATGCATCAAAACATCCTAGTCCCAATGCCGGTTTTTCCGAATCAGTCATGGCCGGAATTTT
GAATATCAAATTGGGTGGTGTCAACTATTACTCCGGAAATAAATCCTTCCGACATTATATGAATGAAACCGGGCATAGTC
CTGACCATTTAGTATTAAGACAAGCAATTAATATAGTTCATCTTACAGCATGGATTGGACTAGCTATCATGGCTAGCTTG
AAGTATTTACTGTGA

Upstream 100 bases:

>100_bases
AAGATGACCCAGTTGGGAAGATTGTATTAAAAGAAGATTGTATTAAAAGAAGATTGTATAAACAAATTAATTAAGATGTA
ATACGGAGTGGGTGTTTTAT

Downstream 100 bases:

>100_bases
TATGGGAGAGGTGATAACAGTGGATAAGATCAAAGAGATAATCGGGAAGTTTTTAGGTGCTACTTCGTTGCTAACGAGAA
TTCCCGTTCCTGTTTGGGGA

Product: cobalamin biosynthesis protein CobD

Products: ADP; phosphate; Adenosyl cobinamide phosphate; Adenosyl cobinamide

Alternate protein names: NA

Number of amino acids: Translated: 324; Mature: 324

Protein sequence:

>324_residues
MEIIGTSILIVIFAFLIDSLLGDFLGRYHPVCLIALLINFLWEKIVTISTSWGFNFLLEGNNFSGLAKFAGFLITIIVVG
VTFTLSYYLASMHFIIDILLIYFALSYKSLVEHLARVLCPLSHGDLIKAREELSKIVGRDVDYLEEAEIARGGIETSAES
LCDGIIAPLFFAFIGGAPLVMSYKAVNTLDSMIGYNFYPYKDLGFASAKLDDVLNLIPARLTAVFILITSLLTKKNFVKG
LQVMRHDASKHPSPNAGFSESVMAGILNIKLGGVNYYSGNKSFRHYMNETGHSPDHLVLRQAINIVHLTAWIGLAIMASL
KYLL

Sequences:

>Translated_324_residues
MEIIGTSILIVIFAFLIDSLLGDFLGRYHPVCLIALLINFLWEKIVTISTSWGFNFLLEGNNFSGLAKFAGFLITIIVVG
VTFTLSYYLASMHFIIDILLIYFALSYKSLVEHLARVLCPLSHGDLIKAREELSKIVGRDVDYLEEAEIARGGIETSAES
LCDGIIAPLFFAFIGGAPLVMSYKAVNTLDSMIGYNFYPYKDLGFASAKLDDVLNLIPARLTAVFILITSLLTKKNFVKG
LQVMRHDASKHPSPNAGFSESVMAGILNIKLGGVNYYSGNKSFRHYMNETGHSPDHLVLRQAINIVHLTAWIGLAIMASL
KYLL
>Mature_324_residues
MEIIGTSILIVIFAFLIDSLLGDFLGRYHPVCLIALLINFLWEKIVTISTSWGFNFLLEGNNFSGLAKFAGFLITIIVVG
VTFTLSYYLASMHFIIDILLIYFALSYKSLVEHLARVLCPLSHGDLIKAREELSKIVGRDVDYLEEAEIARGGIETSAES
LCDGIIAPLFFAFIGGAPLVMSYKAVNTLDSMIGYNFYPYKDLGFASAKLDDVLNLIPARLTAVFILITSLLTKKNFVKG
LQVMRHDASKHPSPNAGFSESVMAGILNIKLGGVNYYSGNKSFRHYMNETGHSPDHLVLRQAINIVHLTAWIGLAIMASL
KYLL

Specific function: Converts cobyric acid to cobinamide by the addition of aminopropanol on the F carboxylic group [H]

COG id: COG1270

COG function: function code H; Cobalamin biosynthesis protein CobD/CbiB

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobD/CbiB family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004485 [H]

Pfam domain/function: PF03186 CobD_Cbib [H]

EC number: 6.3.1.10

Molecular weight: Translated: 35828; Mature: 35828

Theoretical pI: Translated: 7.07; Mature: 7.07

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEIIGTSILIVIFAFLIDSLLGDFLGRYHPVCLIALLINFLWEKIVTISTSWGFNFLLEG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
NNFSGLAKFAGFLITIIVVGVTFTLSYYLASMHFIIDILLIYFALSYKSLVEHLARVLCP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LSHGDLIKAREELSKIVGRDVDYLEEAEIARGGIETSAESLCDGIIAPLFFAFIGGAPLV
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
MSYKAVNTLDSMIGYNFYPYKDLGFASAKLDDVLNLIPARLTAVFILITSLLTKKNFVKG
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQVMRHDASKHPSPNAGFSESVMAGILNIKLGGVNYYSGNKSFRHYMNETGHSPDHLVLR
HHHHHHHHHCCCCCCCCCHHHHHHHHHHEEECCEEEECCCHHHHHHHHHCCCCCHHHHHH
QAINIVHLTAWIGLAIMASLKYLL
HHHHHHHHHHHHHHHHHHHHHHHC
>Mature Secondary Structure
MEIIGTSILIVIFAFLIDSLLGDFLGRYHPVCLIALLINFLWEKIVTISTSWGFNFLLEG
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEEEEC
NNFSGLAKFAGFLITIIVVGVTFTLSYYLASMHFIIDILLIYFALSYKSLVEHLARVLCP
CCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
LSHGDLIKAREELSKIVGRDVDYLEEAEIARGGIETSAESLCDGIIAPLFFAFIGGAPLV
CCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCHHH
MSYKAVNTLDSMIGYNFYPYKDLGFASAKLDDVLNLIPARLTAVFILITSLLTKKNFVKG
HHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LQVMRHDASKHPSPNAGFSESVMAGILNIKLGGVNYYSGNKSFRHYMNETGHSPDHLVLR
HHHHHHHHHCCCCCCCCCHHHHHHHHHHEEECCEEEECCCHHHHHHHHHCCCCCHHHHHH
QAINIVHLTAWIGLAIMASLKYLL
HHHHHHHHHHHHHHHHHHHHHHHC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; adenosylcobyric acid; (R)-1-aminopropan-2-yl phosphate; (R)-1-aminopropan-2-ol

Specific reaction: (1) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-yl phosphate = ADP + phosphate + adenosylcobinamide phosphate; (2) ATP + adenosylcobyric acid + (R)-1-aminopropan-2-ol = ADP + phosphate + adenosylcobinamide

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11792842 [H]