Click here to switch to the map view.

The map label for this gene is cobS [H]

Identifier: 188585569

GI number: 188585569

Start: 997642

End: 998460

Strand: Direct

Name: cobS [H]

Synonym: Nther_0941

Alternate gene names: 188585569

Gene position: 997642-998460 (Clockwise)

Preceding gene: 188585568

Following gene: 188585570

Centisome position: 31.52

GC content: 36.02

Gene sequence:

>819_bases
GTGGATAAGATCAAAGAGATAATCGGGAAGTTTTTAGGTGCTACTTCGTTGCTAACGAGAATTCCCGTTCCTGTTTGGGG
ATTTAGCTATCCTAATCCTTCTTGGACGCCTTATTTTCCTTTTGTAGGTCTTACTCTGGGAACAATTTATGTGATTGTAG
ATATGTTGATAAGTCCTCTACTGGTACCGGAAATTATGGCTTTTTTTATGGTATTACTTGATATATTTTTGTCCGGTGGC
TTACATTTGGACGGTTTGGCTGATAGTTTTGATGGATATTTTAGCGGCAAGACTGGTACTGAATTGCTTGAAATTATGAG
GGATAGCAATGTTGGAGCATATGGTGTAATAGCTCTATGGATCTTATTATCTGGCAAATGGCTATTATTAAGTTTTATTA
TTTCAATGGGTGAACCCATCTGGTTGTTGTTATTTCCTTGGGCAGGAAGATTGATTATGTGGCAGGTGTTAATATCTTAT
CCATATCCTGAAGGTGTTCGCGGGATGGGAGAAATTATAAAAGACTCAAAAGGTTTTAAAACAAGACAGGCCCTTATGGT
ACCGGTATTTAGTATAGTAATTGTTCTGTCTCTTTCTCTTTTGCTGACTGGTTTACAATATGATGAGCAATTAAACTTAA
GCAATTATTTCTTAGTCATTCTGACCATTGTTGCAACTATTTTAGTTGTCGGGATTGTGACTAATATTTTTGTTAAAAAT
TTTAATGATAAACTAGGTGGAGTTACTGGGGATATATACGGAGCCAGTTGTGAAATTGGACAATTACTGGTTTTATCTTT
TGGCGCAATATTAATGTGA

Upstream 100 bases:

>100_bases
TTAAGACAAGCAATTAATATAGTTCATCTTACAGCATGGATTGGACTAGCTATCATGGCTAGCTTGAAGTATTTACTGTG
ATATGGGAGAGGTGATAACA

Downstream 100 bases:

>100_bases
ATTGTTGATATAACTTCCGGAATATAAATTGTGGGAAATATAAGTTGGATGTAATATAATTCAGAATATATAAGTTGCAG
AATAGGTGGGATTGCCATGA

Product: cobalamin 5'-phosphate synthase

Products: GMP; adenosylcobalamin

Alternate protein names: NA

Number of amino acids: Translated: 272; Mature: 272

Protein sequence:

>272_residues
MDKIKEIIGKFLGATSLLTRIPVPVWGFSYPNPSWTPYFPFVGLTLGTIYVIVDMLISPLLVPEIMAFFMVLLDIFLSGG
LHLDGLADSFDGYFSGKTGTELLEIMRDSNVGAYGVIALWILLSGKWLLLSFIISMGEPIWLLLFPWAGRLIMWQVLISY
PYPEGVRGMGEIIKDSKGFKTRQALMVPVFSIVIVLSLSLLLTGLQYDEQLNLSNYFLVILTIVATILVVGIVTNIFVKN
FNDKLGGVTGDIYGASCEIGQLLVLSFGAILM

Sequences:

>Translated_272_residues
MDKIKEIIGKFLGATSLLTRIPVPVWGFSYPNPSWTPYFPFVGLTLGTIYVIVDMLISPLLVPEIMAFFMVLLDIFLSGG
LHLDGLADSFDGYFSGKTGTELLEIMRDSNVGAYGVIALWILLSGKWLLLSFIISMGEPIWLLLFPWAGRLIMWQVLISY
PYPEGVRGMGEIIKDSKGFKTRQALMVPVFSIVIVLSLSLLLTGLQYDEQLNLSNYFLVILTIVATILVVGIVTNIFVKN
FNDKLGGVTGDIYGASCEIGQLLVLSFGAILM
>Mature_272_residues
MDKIKEIIGKFLGATSLLTRIPVPVWGFSYPNPSWTPYFPFVGLTLGTIYVIVDMLISPLLVPEIMAFFMVLLDIFLSGG
LHLDGLADSFDGYFSGKTGTELLEIMRDSNVGAYGVIALWILLSGKWLLLSFIISMGEPIWLLLFPWAGRLIMWQVLISY
PYPEGVRGMGEIIKDSKGFKTRQALMVPVFSIVIVLSLSLLLTGLQYDEQLNLSNYFLVILTIVATILVVGIVTNIFVKN
FNDKLGGVTGDIYGASCEIGQLLVLSFGAILM

Specific function: Joins Ado-cobinamide-GDP and alpha-ribazole to generate adenosylcobalamin (Ado-cobalamin) [H]

COG id: COG0368

COG function: function code H; Cobalamin-5-phosphate synthase

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the CobS family [H]

Homologues:

Organism=Escherichia coli, GI1788301, Length=257, Percent_Identity=32.295719844358, Blast_Score=94, Evalue=8e-21,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003805 [H]

Pfam domain/function: PF02654 CobS [H]

EC number: 2.7.8.26

Molecular weight: Translated: 30018; Mature: 30018

Theoretical pI: Translated: 4.61; Mature: 4.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.7 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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CCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC
>Mature Secondary Structure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CCHHHCCCCCCCCCCCCHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: adenosylcobinamide-GDP; alpha-ribazole

Specific reaction: adenosylcobinamide-GDP + alpha-ribazole = GMP + adenosylcobalamin

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: NA