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The map label for this gene is 188585426

Identifier: 188585426

GI number: 188585426

Start: 830208

End: 831533

Strand: Direct

Name: 188585426

Synonym: Nther_0796

Alternate gene names: NA

Gene position: 830208-831533 (Clockwise)

Preceding gene: 188585425

Following gene: 188585427

Centisome position: 26.23

GC content: 33.26

Gene sequence:

>1326_bases
ATGGAGAAATTTAAGCAATACAAAAAATACAAGGACTCTGGTATTGAATGGCTTGGAAAAGTTCCGAGTCACTGGGACAT
AAACCGGATGGATGCTTATACAAAATATTACAAGAAAAGTATTGAACGAGAAGCACTTCGCGGAAAGACAGTTTTTTACT
ATAGTATTCCTGCAATTGAAGAAACAGGCGATGGTGTGGTAGAAGAAGGCTCAAACATTGATAGTAATAAATTACTACTT
AAGGGAGAAGAATTACTGGTATCAAAGTTGAACCCAAGAAAAGGTAGAATAATTCCTACTAAAGAAAAAGAAATGCCAAT
TATTTGCTCTTCAGAATTTGTTCCCCTGGTACCCCGCAATTGTAGTAGGGAATTTATTAGATATATATATCAGTCAGAAC
TAGTAAAACAAAAACTAAGTAGTGCTGTACAATCTGCTACTAATAGTCATCAAAGAGTTAACCCTAGAGATATATCAAAA
ATATATTTTGCGTTTCCAAGTAAAAGTGAACAGGATAATATAGTAAAATACTTAAATTCAAAAACATCTCAAATAGATTC
CCTAATCAACAAAAAACAAAACCTCATCGAAAAACTCCAAGAATACAAGCAATCCCTTATAACCCACACCGTCACCAAAG
GACTTGACCCCAATGTAAAAATGAAAGATTCGGGTGTTGAATGGATAGGGGAAGTGCCGGAGCATTGGGAGATTTTAAAA
GGGAAATATCTGCTAGATATTTATAACGGGTATCCTCCTGAAGAATTAAGTTTAAGTGCTAATGGTCAGGTGAAATATAT
TCAAGTGGATGACTTAAACACAGAAAATGATGAATTAGTAATAAAAGATTCTAAGTTAAAACTTAAAAATAAAAAGACAG
AAGCATTAGATCACCCAATTATATTGATACCTAAAAGAGGTGCTGCAATTTTTACAAATAAAGTCAAGATTTTAGTTGAT
AAAGGACTTATTGACTCTAATATAATGGGGTTAAAACCTAAGAAAAACTGTAATATACATTATTTAGTCTATATGATTAA
GGCGAGGAAAGTAGATGATATTGCGGATACATCTACAATACCTCAAATTAACAATAAGCATATTAACCCTCTACCACTAA
CTATACCACCCATCGAAGAACAAAATAAAATAGCAGAATATCTAGATGAAAAAGTTGATAATATAAATAATTGTATTCTT
AATATAAAAGTAGCTATCCAAAAACTCAAAGAATACCGCCAATCCCTTATCACCCACGCAGTCACTGGCAAGATTGACGT
CAGGGACTGGGCAGATGCAAAGGAAGGTGAAGATAATGTCTGTTGA

Upstream 100 bases:

>100_bases
TTTTATAAGTATGAACCACCTAGAGAACCAGAGGTTATTATGGAAGAAATAATTGAATTGGAACATGACATCAAAGAAGA
ACTGAAGAAGGTGATAGGCA

Downstream 100 bases:

>100_bases
TCACAGTGAGATCTCCCTTGAAAAAACTATAGAATCTTATCTAATAAACCATGGTGGATATATTAAAGGTGACCCCTCTG
AATTTGCTAGGGAAAAAGCA

Product: restriction modification system DNA specificity domain

Products: NA

Alternate protein names: S.MjaXIP; Type I restriction enzyme MjaXIP specificity protein; S protein [H]

Number of amino acids: Translated: 441; Mature: 441

Protein sequence:

>441_residues
MEKFKQYKKYKDSGIEWLGKVPSHWDINRMDAYTKYYKKSIEREALRGKTVFYYSIPAIEETGDGVVEEGSNIDSNKLLL
KGEELLVSKLNPRKGRIIPTKEKEMPIICSSEFVPLVPRNCSREFIRYIYQSELVKQKLSSAVQSATNSHQRVNPRDISK
IYFAFPSKSEQDNIVKYLNSKTSQIDSLINKKQNLIEKLQEYKQSLITHTVTKGLDPNVKMKDSGVEWIGEVPEHWEILK
GKYLLDIYNGYPPEELSLSANGQVKYIQVDDLNTENDELVIKDSKLKLKNKKTEALDHPIILIPKRGAAIFTNKVKILVD
KGLIDSNIMGLKPKKNCNIHYLVYMIKARKVDDIADTSTIPQINNKHINPLPLTIPPIEEQNKIAEYLDEKVDNINNCIL
NIKVAIQKLKEYRQSLITHAVTGKIDVRDWADAKEGEDNVC

Sequences:

>Translated_441_residues
MEKFKQYKKYKDSGIEWLGKVPSHWDINRMDAYTKYYKKSIEREALRGKTVFYYSIPAIEETGDGVVEEGSNIDSNKLLL
KGEELLVSKLNPRKGRIIPTKEKEMPIICSSEFVPLVPRNCSREFIRYIYQSELVKQKLSSAVQSATNSHQRVNPRDISK
IYFAFPSKSEQDNIVKYLNSKTSQIDSLINKKQNLIEKLQEYKQSLITHTVTKGLDPNVKMKDSGVEWIGEVPEHWEILK
GKYLLDIYNGYPPEELSLSANGQVKYIQVDDLNTENDELVIKDSKLKLKNKKTEALDHPIILIPKRGAAIFTNKVKILVD
KGLIDSNIMGLKPKKNCNIHYLVYMIKARKVDDIADTSTIPQINNKHINPLPLTIPPIEEQNKIAEYLDEKVDNINNCIL
NIKVAIQKLKEYRQSLITHAVTGKIDVRDWADAKEGEDNVC
>Mature_441_residues
MEKFKQYKKYKDSGIEWLGKVPSHWDINRMDAYTKYYKKSIEREALRGKTVFYYSIPAIEETGDGVVEEGSNIDSNKLLL
KGEELLVSKLNPRKGRIIPTKEKEMPIICSSEFVPLVPRNCSREFIRYIYQSELVKQKLSSAVQSATNSHQRVNPRDISK
IYFAFPSKSEQDNIVKYLNSKTSQIDSLINKKQNLIEKLQEYKQSLITHTVTKGLDPNVKMKDSGVEWIGEVPEHWEILK
GKYLLDIYNGYPPEELSLSANGQVKYIQVDDLNTENDELVIKDSKLKLKNKKTEALDHPIILIPKRGAAIFTNKVKILVD
KGLIDSNIMGLKPKKNCNIHYLVYMIKARKVDDIADTSTIPQINNKHINPLPLTIPPIEEQNKIAEYLDEKVDNINNCIL
NIKVAIQKLKEYRQSLITHAVTGKIDVRDWADAKEGEDNVC

Specific function: The M and S subunits together form a methyltransferase (MTase) that methylates two adenine residues in complementary strands of a bipartite DNA recognition sequence. In the presence of the R subunit the complex can also act as an endonuclease, binding to

COG id: COG0732

COG function: function code V; Restriction endonuclease S subunits

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the type-I restriction system S methylase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000055 [H]

Pfam domain/function: PF01420 Methylase_S [H]

EC number: NA

Molecular weight: Translated: 50608; Mature: 50608

Theoretical pI: Translated: 9.14; Mature: 9.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEKFKQYKKYKDSGIEWLGKVPSHWDINRMDAYTKYYKKSIEREALRGKTVFYYSIPAIE
CCHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
ETGDGVVEEGSNIDSNKLLLKGEELLVSKLNPRKGRIIPTKEKEMPIICSSEFVPLVPRN
CCCCCHHHCCCCCCCCEEEEECHHHHHHCCCCCCCCEECCCCCCCCEEECCCCCCCCCCC
CSREFIRYIYQSELVKQKLSSAVQSATNSHQRVNPRDISKIYFAFPSKSEQDNIVKYLNS
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEECCCCCCHHHHHHHHCC
KTSQIDSLINKKQNLIEKLQEYKQSLITHTVTKGLDPNVKMKDSGVEWIGEVPEHWEILK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHCCCHHHHHHC
GKYLLDIYNGYPPEELSLSANGQVKYIQVDDLNTENDELVIKDSKLKLKNKKTEALDHPI
CCEEEEECCCCCCCCEEECCCCCEEEEEEECCCCCCCEEEEECCCEEECCCHHHHCCCCE
ILIPKRGAAIFTNKVKILVDKGLIDSNIMGLKPKKNCNIHYLVYMIKARKVDDIADTSTI
EEEECCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHCCCCC
PQINNKHINPLPLTIPPIEEQNKIAEYLDEKVDNINNCILNIKVAIQKLKEYRQSLITHA
CCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHH
VTGKIDVRDWADAKEGEDNVC
HCCCEECCCCCCCCCCCCCCC
>Mature Secondary Structure
MEKFKQYKKYKDSGIEWLGKVPSHWDINRMDAYTKYYKKSIEREALRGKTVFYYSIPAIE
CCHHHHHHHHHHCCHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCC
ETGDGVVEEGSNIDSNKLLLKGEELLVSKLNPRKGRIIPTKEKEMPIICSSEFVPLVPRN
CCCCCHHHCCCCCCCCEEEEECHHHHHHCCCCCCCCEECCCCCCCCEEECCCCCCCCCCC
CSREFIRYIYQSELVKQKLSSAVQSATNSHQRVNPRDISKIYFAFPSKSEQDNIVKYLNS
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHCCEEEEECCCCCCHHHHHHHHCC
KTSQIDSLINKKQNLIEKLQEYKQSLITHTVTKGLDPNVKMKDSGVEWIGEVPEHWEILK
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCHHHHCCCHHHHHHC
GKYLLDIYNGYPPEELSLSANGQVKYIQVDDLNTENDELVIKDSKLKLKNKKTEALDHPI
CCEEEEECCCCCCCCEEECCCCCEEEEEEECCCCCCCEEEEECCCEEECCCHHHHCCCCE
ILIPKRGAAIFTNKVKILVDKGLIDSNIMGLKPKKNCNIHYLVYMIKARKVDDIADTSTI
EEEECCCCEEEECCEEEEEECCCCCCCCCCCCCCCCCCEEEEEEEEECCCCCHHHCCCCC
PQINNKHINPLPLTIPPIEEQNKIAEYLDEKVDNINNCILNIKVAIQKLKEYRQSLITHA
CCCCCCCCCCCCEEECCCCHHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHH
VTGKIDVRDWADAKEGEDNVC
HCCCEECCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087 [H]