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The map label for this gene is amhX [H]

Identifier: 188585411

GI number: 188585411

Start: 813752

End: 814861

Strand: Direct

Name: amhX [H]

Synonym: Nther_0781

Alternate gene names: 188585411

Gene position: 813752-814861 (Clockwise)

Preceding gene: 188585410

Following gene: 188585417

Centisome position: 25.71

GC content: 38.2

Gene sequence:

>1110_bases
ATGACTACAAATAAGTTAGAAGAATACGTGTTCAGGGTATTTGAAGAACTTCATAATATGCCTGAAGTAGGATATGATGA
GTATAAGACTTCTGCTTTTATAGCCAATGAATTAAAGGAAATGGGATATACTGTTTTAGATAAAATTAATGGTACAGGTG
TAATAGGTATATTGGATAGCAAAAATCCGGGTCCAATCTTTGCGTTAAGAGCTGATATGGATGCATTAGAATTTGAAAAG
GATGGCGAGAAATATAATCTACATGCCTGTGGACATGATGCCAATGCGTCAATGGTTCTTGGGGCGGCCAAGCAGATTAT
CGAAACAGGAATTAGTACAGGCAAGATCTATGTAGTCTTCCAACCTGCAGAAGAAAAATTAGGAGGCTCTAAAAGTATCA
TAGAAAGTGGTCACATTAATGAAGTTGAAGAAATGGTGGGTATCCATTTAAGACCTATTCAAGAGGCTAAGTTAGGGGAG
GCTACTCCAGCACTGATACATGGATCAAGTAAAAGAGTAGATATAACTGTTAAAGGTTTAAACTCTCATGGTGCAAGACC
TCATTTAGGAGTGAATGCTGTTGACGCGGGTGTTGCAATAACTAACGCTATTAACAGTATAAAAGCTAATCCTGCTGTAT
CTTACTCTGTTAAAGTAACTTCATTTAAAGCCGGAGGAGAAGTATTTAATATTATTCCCGATAGAGCTGTGTTAACAGTT
GATATTAGGGCTCAAACCAATGAAGTAATGGATGATATTCGTACTAAGCTAGATTATGCCATAGAGAATGGTGCTAAAAC
TGTAGGAGCACAATCCCAAATTGACTTTGAAGGAGGAGTCCCAGCTGCCGAGCACAACGAGGATTTAATCCAGTTAGCAG
ATGAAGTTATTAAGGAAGAACTCGGCAAGTCACTAGATCCCATGCTTACACCGGGAGCTGAAGATTTTCATTTTTATTCC
CAAGATCTAGGGATAAAAACAACCTATATCGGCTTAGGTGCAGATTTGAAACCTGGGCTCCATCATCCCGATATGAGTTT
TGACAAGTCAGCACTAATTAAAGGATGTAATATTCTCGTTAAAATGACCAAAAAAAGGCTGGAAGATTGA

Upstream 100 bases:

>100_bases
ATATGCCAAAGGGGAGTTAATGGTATCTCACAAAAAAGTATTAAAGAAAGGGACATTTGAGTAATACTTTCTAGAGTAAT
CAAATGTCAAGGTGAAAATT

Downstream 100 bases:

>100_bases
TATCTCAGCTCTGTATTTAAGATAAAGGGAACTATTGGATGCTCAAAGGTTAAAAAATGATTATATAAAATAAGGAAAGG
ACTCGCTAATTAAAGCGAGT

Product: amidohydrolase

Products: NA

Alternate protein names: Aminoacylase [H]

Number of amino acids: Translated: 369; Mature: 368

Protein sequence:

>369_residues
MTTNKLEEYVFRVFEELHNMPEVGYDEYKTSAFIANELKEMGYTVLDKINGTGVIGILDSKNPGPIFALRADMDALEFEK
DGEKYNLHACGHDANASMVLGAAKQIIETGISTGKIYVVFQPAEEKLGGSKSIIESGHINEVEEMVGIHLRPIQEAKLGE
ATPALIHGSSKRVDITVKGLNSHGARPHLGVNAVDAGVAITNAINSIKANPAVSYSVKVTSFKAGGEVFNIIPDRAVLTV
DIRAQTNEVMDDIRTKLDYAIENGAKTVGAQSQIDFEGGVPAAEHNEDLIQLADEVIKEELGKSLDPMLTPGAEDFHFYS
QDLGIKTTYIGLGADLKPGLHHPDMSFDKSALIKGCNILVKMTKKRLED

Sequences:

>Translated_369_residues
MTTNKLEEYVFRVFEELHNMPEVGYDEYKTSAFIANELKEMGYTVLDKINGTGVIGILDSKNPGPIFALRADMDALEFEK
DGEKYNLHACGHDANASMVLGAAKQIIETGISTGKIYVVFQPAEEKLGGSKSIIESGHINEVEEMVGIHLRPIQEAKLGE
ATPALIHGSSKRVDITVKGLNSHGARPHLGVNAVDAGVAITNAINSIKANPAVSYSVKVTSFKAGGEVFNIIPDRAVLTV
DIRAQTNEVMDDIRTKLDYAIENGAKTVGAQSQIDFEGGVPAAEHNEDLIQLADEVIKEELGKSLDPMLTPGAEDFHFYS
QDLGIKTTYIGLGADLKPGLHHPDMSFDKSALIKGCNILVKMTKKRLED
>Mature_368_residues
TTNKLEEYVFRVFEELHNMPEVGYDEYKTSAFIANELKEMGYTVLDKINGTGVIGILDSKNPGPIFALRADMDALEFEKD
GEKYNLHACGHDANASMVLGAAKQIIETGISTGKIYVVFQPAEEKLGGSKSIIESGHINEVEEMVGIHLRPIQEAKLGEA
TPALIHGSSKRVDITVKGLNSHGARPHLGVNAVDAGVAITNAINSIKANPAVSYSVKVTSFKAGGEVFNIIPDRAVLTVD
IRAQTNEVMDDIRTKLDYAIENGAKTVGAQSQIDFEGGVPAAEHNEDLIQLADEVIKEELGKSLDPMLTPGAEDFHFYSQ
DLGIKTTYIGLGADLKPGLHHPDMSFDKSALIKGCNILVKMTKKRLED

Specific function: Required But Not Essential For Aminobenzoyl-Glutamate Utilization. May Participate In Hydrolysis Of Aminobenzoyl- Glutamate To Aminobenzoate, Either Alone Or In Combination With Abgb. [C]

COG id: COG1473

COG function: function code R; Metal-dependent amidase/aminoacylase/carboxypeptidase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M20 family [H]

Homologues:

Organism=Escherichia coli, GI87081880, Length=428, Percent_Identity=23.3644859813084, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017439
- InterPro:   IPR010168
- InterPro:   IPR002933
- InterPro:   IPR011650 [H]

Pfam domain/function: PF07687 M20_dimer; PF01546 Peptidase_M20 [H]

EC number: NA

Molecular weight: Translated: 40006; Mature: 39875

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.3 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTTNKLEEYVFRVFEELHNMPEVGYDEYKTSAFIANELKEMGYTVLDKINGTGVIGILDS
CCCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEEEEC
KNPGPIFALRADMDALEFEKDGEKYNLHACGHDANASMVLGAAKQIIETGISTGKIYVVF
CCCCCEEEEECCCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEE
QPAEEKLGGSKSIIESGHINEVEEMVGIHLRPIQEAKLGEATPALIHGSSKRVDITVKGL
ECCHHHCCCCHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCEEEEEEEEC
NSHGARPHLGVNAVDAGVAITNAINSIKANPAVSYSVKVTSFKAGGEVFNIIPDRAVLTV
CCCCCCCCCCCCHHHCCHHHHHHHHHCCCCCCEEEEEEEEEEECCCCEEEECCCCEEEEE
DIRAQTNEVMDDIRTKLDYAIENGAKTVGAQSQIDFEGGVPAAEHNEDLIQLADEVIKEE
EECCCHHHHHHHHHHHHHHHHHCCCHHCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHH
LGKSLDPMLTPGAEDFHFYSQDLGIKTTYIGLGADLKPGLHHPDMSFDKSALIKGCNILV
HCCCCCCCCCCCCCHHEEHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KMTKKRLED
HHHHHHCCC
>Mature Secondary Structure 
TTNKLEEYVFRVFEELHNMPEVGYDEYKTSAFIANELKEMGYTVLDKINGTGVIGILDS
CCHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHCCHHHHHCCCCCEEEEEEEC
KNPGPIFALRADMDALEFEKDGEKYNLHACGHDANASMVLGAAKQIIETGISTGKIYVVF
CCCCCEEEEECCCCHHHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHCCCCCCEEEEEE
QPAEEKLGGSKSIIESGHINEVEEMVGIHLRPIQEAKLGEATPALIHGSSKRVDITVKGL
ECCHHHCCCCHHHHCCCCHHHHHHHHCCCCCCCCCCCCCCCCCCEEECCCCEEEEEEEEC
NSHGARPHLGVNAVDAGVAITNAINSIKANPAVSYSVKVTSFKAGGEVFNIIPDRAVLTV
CCCCCCCCCCCCHHHCCHHHHHHHHHCCCCCCEEEEEEEEEEECCCCEEEECCCCEEEEE
DIRAQTNEVMDDIRTKLDYAIENGAKTVGAQSQIDFEGGVPAAEHNEDLIQLADEVIKEE
EECCCHHHHHHHHHHHHHHHHHCCCHHCCCCCEECCCCCCCCCCCCHHHHHHHHHHHHHH
LGKSLDPMLTPGAEDFHFYSQDLGIKTTYIGLGADLKPGLHHPDMSFDKSALIKGCNILV
HCCCCCCCCCCCCCHHEEHHHCCCCEEEEEECCCCCCCCCCCCCCCCCHHHHHHHHHHHH
KMTKKRLED
HHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8768514; 8969502; 9384377; 7622480 [H]