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The map label for this gene is iadA [H]

Identifier: 188585410

GI number: 188585410

Start: 812549

End: 813715

Strand: Direct

Name: iadA [H]

Synonym: Nther_0780

Alternate gene names: 188585410

Gene position: 812549-813715 (Clockwise)

Preceding gene: 188585409

Following gene: 188585411

Centisome position: 25.67

GC content: 35.9

Gene sequence:

>1167_bases
ATGCTAACACTAATTAAAAATGCTCAACTTTACACACCGGAATATTTAGGTAAGAAAGATATTTTATTGGCAAGTGATAA
GATTCAGATGATATCAGATGAAATTAACTTGTCTGAAAACTCGGGGTTAGAGCTAGAAATAATTGATTCAGAGGGCTATT
GGGTAGTACCAGGATTTATAGATAACCATGTACATCTGATTGGTGGCGGAGGTGAAGGAGGATATAATACTCGAACTCCT
GAAATTCAGCTTTCAAAACTCACTGAAGCTGGCATTACAACAGTAATAGGTGTTTTGGGTACAGATGGAACCACTAGACA
TATGACCAGTCTTCTAGCAAAAGCTAGAGCTTTAGAGTCAGAAGGAATATCAACCTATATTCTGACCGGTTCATATCAAA
TACCCGTTCACACTATTACCGGTAATGTTAGAGATGATCTTGTAATAATTGATAAAGTAATTGGTGTTGGTGAAATTGCT
ATTTCAGATCACAGATCTTCTAAGCCAACATTGGAAGAAATTAAAAAGCTTTCTGGAGAAGCAAGGGTAGGTGGCATGCT
TTCTGGGAAACCAGGAGTAATAAATTTACACCTAGGTGATGAAAAGGATGGACTACGTTATTTGAACCAAATTGTAGAAA
ACACTGATATTCCATGCACTCAGTTTTTGCCAACTCACATTAATAGAAGCGAAAGACTGTTAGAAGAAGGTGTGGAGTAT
ACAAGAAACGGTGGTTACATTGACTTAACCACTAGTGGGGCCTCACAAAAAATAATGTTAAAACCAAGTAAAATATTGAG
AAAATTGAAAGATAGTGGTGTAGATTTAAGTAAAGTCACCTTGAGTTCTGATGGACAGGGAAGTTTACCATCCTTTGATG
AAGAAGGGAATTTTAAGGGACTAGGGATTGGTTCTGTTAACTCCCTCTATTCTGAAGTTAGAGATGCAATATTGCATGAA
AAATTAGAACCTCAGGAAGCTATAAGTACTATCACCCAGAATGTAGCTGAGATTTATAAATTAGCTGACCAAGGAAGAAT
TTTAGAAGGTAATCAAGCTAATTTGGTTTTCTTAGATCAGAATGATTTAACAATTGATTCAGTATATGCCAAAGGGGAGT
TAATGGTATCTCACAAAAAAGTATTAAAGAAAGGGACATTTGAGTAA

Upstream 100 bases:

>100_bases
CTTGATTGCGATGTTAGTTATGAATTATTTAGCTTTACCTCTCTTTTAAAATGAATAATTATTTTCTTGTATCAAAATAG
GGATGTGAAAGGAGTGTATT

Downstream 100 bases:

>100_bases
TACTTTCTAGAGTAATCAAATGTCAAGGTGAAAATTATGACTACAAATAAGTTAGAAGAATACGTGTTCAGGGTATTTGA
AGAACTTCATAATATGCCTG

Product: isoaspartyl dipeptidase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 388; Mature: 388

Protein sequence:

>388_residues
MLTLIKNAQLYTPEYLGKKDILLASDKIQMISDEINLSENSGLELEIIDSEGYWVVPGFIDNHVHLIGGGGEGGYNTRTP
EIQLSKLTEAGITTVIGVLGTDGTTRHMTSLLAKARALESEGISTYILTGSYQIPVHTITGNVRDDLVIIDKVIGVGEIA
ISDHRSSKPTLEEIKKLSGEARVGGMLSGKPGVINLHLGDEKDGLRYLNQIVENTDIPCTQFLPTHINRSERLLEEGVEY
TRNGGYIDLTTSGASQKIMLKPSKILRKLKDSGVDLSKVTLSSDGQGSLPSFDEEGNFKGLGIGSVNSLYSEVRDAILHE
KLEPQEAISTITQNVAEIYKLADQGRILEGNQANLVFLDQNDLTIDSVYAKGELMVSHKKVLKKGTFE

Sequences:

>Translated_388_residues
MLTLIKNAQLYTPEYLGKKDILLASDKIQMISDEINLSENSGLELEIIDSEGYWVVPGFIDNHVHLIGGGGEGGYNTRTP
EIQLSKLTEAGITTVIGVLGTDGTTRHMTSLLAKARALESEGISTYILTGSYQIPVHTITGNVRDDLVIIDKVIGVGEIA
ISDHRSSKPTLEEIKKLSGEARVGGMLSGKPGVINLHLGDEKDGLRYLNQIVENTDIPCTQFLPTHINRSERLLEEGVEY
TRNGGYIDLTTSGASQKIMLKPSKILRKLKDSGVDLSKVTLSSDGQGSLPSFDEEGNFKGLGIGSVNSLYSEVRDAILHE
KLEPQEAISTITQNVAEIYKLADQGRILEGNQANLVFLDQNDLTIDSVYAKGELMVSHKKVLKKGTFE
>Mature_388_residues
MLTLIKNAQLYTPEYLGKKDILLASDKIQMISDEINLSENSGLELEIIDSEGYWVVPGFIDNHVHLIGGGGEGGYNTRTP
EIQLSKLTEAGITTVIGVLGTDGTTRHMTSLLAKARALESEGISTYILTGSYQIPVHTITGNVRDDLVIIDKVIGVGEIA
ISDHRSSKPTLEEIKKLSGEARVGGMLSGKPGVINLHLGDEKDGLRYLNQIVENTDIPCTQFLPTHINRSERLLEEGVEY
TRNGGYIDLTTSGASQKIMLKPSKILRKLKDSGVDLSKVTLSSDGQGSLPSFDEEGNFKGLGIGSVNSLYSEVRDAILHE
KLEPQEAISTITQNVAEIYKLADQGRILEGNQANLVFLDQNDLTIDSVYAKGELMVSHKKVLKKGTFE

Specific function: Catalyzes the hydrolytic cleavage of a subset of L- isoaspartyl (L-beta-aspartyl) dipeptides. Used to degrade proteins damaged by L-isoaspartyl residues formation. The best substrate for the enzyme reported thus far is iso-Asp-Leu [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M38 family [H]

Homologues:

Organism=Escherichia coli, GI1790784, Length=387, Percent_Identity=45.2196382428941, Blast_Score=342, Evalue=2e-95,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006680
- InterPro:   IPR011059
- InterPro:   IPR010229 [H]

Pfam domain/function: PF01979 Amidohydro_1 [H]

EC number: 3.4.19.- [C]

Molecular weight: Translated: 42251; Mature: 42251

Theoretical pI: Translated: 4.91; Mature: 4.91

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLTLIKNAQLYTPEYLGKKDILLASDKIQMISDEINLSENSGLELEIIDSEGYWVVPGFI
CEEEEECCEEECHHHCCCCCEEEECCCCEEEHHHCCCCCCCCCEEEEECCCCCEEECCEE
DNHVHLIGGGGEGGYNTRTPEIQLSKLTEAGITTVIGVLGTDGTTRHMTSLLAKARALES
CCEEEEEECCCCCCCCCCCCCEEEEHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
EGISTYILTGSYQIPVHTITGNVRDDLVIIDKVIGVGEIAISDHRSSKPTLEEIKKLSGE
CCCEEEEEECCEEEEEEEEECCCCCCEEEEEHHHCCCEEEECCCCCCCCHHHHHHHHCCC
ARVGGMLSGKPGVINLHLGDEKDGLRYLNQIVENTDIPCTQFLPTHINRSERLLEEGVEY
CCCCCEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHCHHHCCHHHHHHHHHHHH
TRNGGYIDLTTSGASQKIMLKPSKILRKLKDSGVDLSKVTLSSDGQGSLPSFDEEGNFKG
HCCCCEEEEEECCCCCEEEECHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEE
LGIGSVNSLYSEVRDAILHEKLEPQEAISTITQNVAEIYKLADQGRILEGNQANLVFLDQ
EECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEECC
NDLTIDSVYAKGELMVSHKKVLKKGTFE
CCCEEEEEEECCEEEEHHHHHHHCCCCC
>Mature Secondary Structure
MLTLIKNAQLYTPEYLGKKDILLASDKIQMISDEINLSENSGLELEIIDSEGYWVVPGFI
CEEEEECCEEECHHHCCCCCEEEECCCCEEEHHHCCCCCCCCCEEEEECCCCCEEECCEE
DNHVHLIGGGGEGGYNTRTPEIQLSKLTEAGITTVIGVLGTDGTTRHMTSLLAKARALES
CCEEEEEECCCCCCCCCCCCCEEEEHHHHCCHHHHHHHHCCCCHHHHHHHHHHHHHHHHC
EGISTYILTGSYQIPVHTITGNVRDDLVIIDKVIGVGEIAISDHRSSKPTLEEIKKLSGE
CCCEEEEEECCEEEEEEEEECCCCCCEEEEEHHHCCCEEEECCCCCCCCHHHHHHHHCCC
ARVGGMLSGKPGVINLHLGDEKDGLRYLNQIVENTDIPCTQFLPTHINRSERLLEEGVEY
CCCCCEECCCCCEEEEEECCCCHHHHHHHHHHHCCCCCHHHHCHHHCCHHHHHHHHHHHH
TRNGGYIDLTTSGASQKIMLKPSKILRKLKDSGVDLSKVTLSSDGQGSLPSFDEEGNFKG
HCCCCEEEEEECCCCCEEEECHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCCCCCCEEE
LGIGSVNSLYSEVRDAILHEKLEPQEAISTITQNVAEIYKLADQGRILEGNQANLVFLDQ
EECCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCEEECCCCCEEEECC
NDLTIDSVYAKGELMVSHKKVLKKGTFE
CCCEEEEEEECCEEEEHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7876157; 7610040; 9278503 [H]