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The map label for this gene is nuoF2 [H]

Identifier: 188585338

GI number: 188585338

Start: 729509

End: 731275

Strand: Direct

Name: nuoF2 [H]

Synonym: Nther_0703

Alternate gene names: 188585338

Gene position: 729509-731275 (Clockwise)

Preceding gene: 188585337

Following gene: 188585339

Centisome position: 23.05

GC content: 39.62

Gene sequence:

>1767_bases
ATGAGAGTTTTGGTTTGTGGTGGTACTGGATGTGTAACATCCGGCTCCCAAGAAGTTAAAGATGTTTTAGAAACAGAATT
GGAAAAAGAGAATATACAAGACTACAAGGTGATTTTCACTGGCTGTCAAGGGTTTTGTGAGCAAGGGCCGTTAATAAGAG
TCGAACAAGATGAGACCTTCTACTGTAATGTTGATGCAGAAGGTGCTAAGAAAATAGTTCACCATCATTTAAAAAACGGC
CAGCTGGTTTCCGAACTCTTGTATGAAAATCATGATGGTCACAAAGTGCAAAAGCGTGAGGAGATAGATTTTTATCAAAA
GCAAGAAAGGGTGGTTATTAACCGGTCGGGATTTATAGATCCAGAAAACATTGATGAATATTTAAATTCAGGAGGTTATC
AGGCTCTGCAATTAGGTCTTACCATGGAGCCTGAAGATATCATAACAGAAATACAAGCTGCTAATCTTAGGGGAAGAGGT
GGGGGAGGCTTTCCTACGGGTTTGAAGTGGGAATATGCAAAAAAAGGCGATGAAAAAACTGAAAAATACGTTGTCTGTAA
TGCCGATGAGGGAGACCCTGGAGCATTCATGGATAGAAGCATTCTAGAAGGTGACCCTCATGTTGTTATCGAGGGTATGG
CCATTGCAGGGTTAGCTATTGGAGCAAATCGAGGAATTATATATGTTAGAGCTGAATATCCTTTGGCTGTTAAAAGGCTT
GAGAAAGCTATTAGTCAGGCAAAGGAATACGGGTATCTGGGAGAAAGAGTTTTAGGTTCAAACTTTTCTTTTGATATTGA
GATCAAACAGGGAGCTGGAGCCTTTGTTTGCGGAGAGGAAACCGCCTTAATTGAAAGTGTTCATGGGAACAGGGGAATGC
CAAGACCTAGACCGCCTTTCCCAGCCGAAAAAGGCCTCTATGATTCCCCAACTATTCTTAATAACGTAGAAACACTAGCA
AATATACCTGTTATTTTGAATTGGGGTGCCCAAAAGTACAATGAACTTGGTACTAAAGATAGTAAAGGAACAAAAGTTTT
TGCCATAACGGGAAAAGTGAGAAATACCGGACTATGTGAAGTTCCCATGGGCATTAAATTGAAAGATTTAATTTTTGATA
TCGGTGGAGGTATTAAGGATGACAAAGAGTTTAAGGCAGTCCAGATAGGTGGACCTTCAGGTGGGTGCTTGCCTCAAGAA
TCCCTAGACTTTCCCATCGAATATGATTCTCTCATAGATGCAGGAGCTATGATGGGTTCAGGGGGACTTGTAGTCATGGA
TACGAGTACTTGTATGGTAGACTTATCCCGGTTCTTTCTAAATTTTACCCAGAGTGAATCCTGTGGTAAATGTAACCCAT
GTCGTGAAGGAACAAAAAGGCTGTTAGAAATATTAAATCGGATTATTGACGGTTATGGAGAAAATGAAGATCTCAAAATC
CTCAATCGATTAGGAAATAATATCATTAATACTTCTCTTTGTGGTTTGGGACAATCAGCACCTAATCCGGTACTAAGTAC
TATCAGGTACTTCGAGAATGAATACCTAGAACATATCAATAATAAGTACTGTCGAGCCGGGGTATGTAAGAATCTTACCA
CAATAGAAGTAGATCCAGAAAAATGTAGTGGGTGTGAAATTTGCAAGCAGATATGCCCCGTTGATGCAATAAAAGGTGAA
AAGTCAGAGGTTCACAATATAGATTCGAAAGTGTGTATTAAGTGTAAAGAATGCATAGATAAATGTCCTCTTGAAGCAAT
TGAATAA

Upstream 100 bases:

>100_bases
ATCACAATACACACGGAAAAGTTACTACTTCTGATGTAAACCATTTGATTAATAGATATGTTAATGGTTCAAATAACGGA
AATGAAGGTGGGGAACAAGG

Downstream 100 bases:

>100_bases
TAACATAACAACAAGTTGGAGGTAGTATTCAAGTGATGACTGACAGTCAAAACCAAAACACAATTACAATCATGGCAGAT
GGTAATTCATATCAAACAGA

Product: NADH dehydrogenase (quinone)

Products: NA

Alternate protein names: NADH dehydrogenase I subunit F 2; NDH-1 subunit F 2 [H]

Number of amino acids: Translated: 588; Mature: 588

Protein sequence:

>588_residues
MRVLVCGGTGCVTSGSQEVKDVLETELEKENIQDYKVIFTGCQGFCEQGPLIRVEQDETFYCNVDAEGAKKIVHHHLKNG
QLVSELLYENHDGHKVQKREEIDFYQKQERVVINRSGFIDPENIDEYLNSGGYQALQLGLTMEPEDIITEIQAANLRGRG
GGGFPTGLKWEYAKKGDEKTEKYVVCNADEGDPGAFMDRSILEGDPHVVIEGMAIAGLAIGANRGIIYVRAEYPLAVKRL
EKAISQAKEYGYLGERVLGSNFSFDIEIKQGAGAFVCGEETALIESVHGNRGMPRPRPPFPAEKGLYDSPTILNNVETLA
NIPVILNWGAQKYNELGTKDSKGTKVFAITGKVRNTGLCEVPMGIKLKDLIFDIGGGIKDDKEFKAVQIGGPSGGCLPQE
SLDFPIEYDSLIDAGAMMGSGGLVVMDTSTCMVDLSRFFLNFTQSESCGKCNPCREGTKRLLEILNRIIDGYGENEDLKI
LNRLGNNIINTSLCGLGQSAPNPVLSTIRYFENEYLEHINNKYCRAGVCKNLTTIEVDPEKCSGCEICKQICPVDAIKGE
KSEVHNIDSKVCIKCKECIDKCPLEAIE

Sequences:

>Translated_588_residues
MRVLVCGGTGCVTSGSQEVKDVLETELEKENIQDYKVIFTGCQGFCEQGPLIRVEQDETFYCNVDAEGAKKIVHHHLKNG
QLVSELLYENHDGHKVQKREEIDFYQKQERVVINRSGFIDPENIDEYLNSGGYQALQLGLTMEPEDIITEIQAANLRGRG
GGGFPTGLKWEYAKKGDEKTEKYVVCNADEGDPGAFMDRSILEGDPHVVIEGMAIAGLAIGANRGIIYVRAEYPLAVKRL
EKAISQAKEYGYLGERVLGSNFSFDIEIKQGAGAFVCGEETALIESVHGNRGMPRPRPPFPAEKGLYDSPTILNNVETLA
NIPVILNWGAQKYNELGTKDSKGTKVFAITGKVRNTGLCEVPMGIKLKDLIFDIGGGIKDDKEFKAVQIGGPSGGCLPQE
SLDFPIEYDSLIDAGAMMGSGGLVVMDTSTCMVDLSRFFLNFTQSESCGKCNPCREGTKRLLEILNRIIDGYGENEDLKI
LNRLGNNIINTSLCGLGQSAPNPVLSTIRYFENEYLEHINNKYCRAGVCKNLTTIEVDPEKCSGCEICKQICPVDAIKGE
KSEVHNIDSKVCIKCKECIDKCPLEAIE
>Mature_588_residues
MRVLVCGGTGCVTSGSQEVKDVLETELEKENIQDYKVIFTGCQGFCEQGPLIRVEQDETFYCNVDAEGAKKIVHHHLKNG
QLVSELLYENHDGHKVQKREEIDFYQKQERVVINRSGFIDPENIDEYLNSGGYQALQLGLTMEPEDIITEIQAANLRGRG
GGGFPTGLKWEYAKKGDEKTEKYVVCNADEGDPGAFMDRSILEGDPHVVIEGMAIAGLAIGANRGIIYVRAEYPLAVKRL
EKAISQAKEYGYLGERVLGSNFSFDIEIKQGAGAFVCGEETALIESVHGNRGMPRPRPPFPAEKGLYDSPTILNNVETLA
NIPVILNWGAQKYNELGTKDSKGTKVFAITGKVRNTGLCEVPMGIKLKDLIFDIGGGIKDDKEFKAVQIGGPSGGCLPQE
SLDFPIEYDSLIDAGAMMGSGGLVVMDTSTCMVDLSRFFLNFTQSESCGKCNPCREGTKRLLEILNRIIDGYGENEDLKI
LNRLGNNIINTSLCGLGQSAPNPVLSTIRYFENEYLEHINNKYCRAGVCKNLTTIEVDPEKCSGCEICKQICPVDAIKGE
KSEVHNIDSKVCIKCKECIDKCPLEAIE

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. The immediate electron acceptor for the enzyme in this species is believed to be ubiquinone. Couples the redox reaction to proton translocat

COG id: COG1894

COG function: function code C; NADH:ubiquinone oxidoreductase, NADH-binding (51 kD) subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 51 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI260656005, Length=439, Percent_Identity=36.2186788154898, Blast_Score=285, Evalue=7e-77,
Organism=Homo sapiens, GI20149568, Length=426, Percent_Identity=36.8544600938967, Blast_Score=285, Evalue=9e-77,
Organism=Escherichia coli, GI1788620, Length=405, Percent_Identity=39.0123456790123, Blast_Score=318, Evalue=4e-88,
Organism=Caenorhabditis elegans, GI17531727, Length=432, Percent_Identity=36.8055555555556, Blast_Score=288, Evalue=4e-78,
Organism=Drosophila melanogaster, GI19920794, Length=370, Percent_Identity=39.4594594594595, Blast_Score=279, Evalue=3e-75,
Organism=Drosophila melanogaster, GI45550452, Length=378, Percent_Identity=39.6825396825397, Blast_Score=277, Evalue=1e-74,
Organism=Drosophila melanogaster, GI24653819, Length=384, Percent_Identity=35.15625, Blast_Score=236, Evalue=3e-62,
Organism=Drosophila melanogaster, GI24653817, Length=355, Percent_Identity=33.8028169014084, Blast_Score=208, Evalue=7e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001949
- InterPro:   IPR019575
- InterPro:   IPR011537
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10589 NADH_4Fe-4S; PF10531 SLBB [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 64635; Mature: 64635

Theoretical pI: Translated: 4.75; Mature: 4.75

Prosite motif: PS00645 COMPLEX1_51K_2 ; PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

4.1 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
5.8 %Cys+Met (Translated Protein)
4.1 %Cys     (Mature Protein)
1.7 %Met     (Mature Protein)
5.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRVLVCGGTGCVTSGSQEVKDVLETELEKENIQDYKVIFTGCQGFCEQGPLIRVEQDETF
CEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHCCCCEEEEECCCEE
YCNVDAEGAKKIVHHHLKNGQLVSELLYENHDGHKVQKREEIDFYQKQERVVINRSGFID
EEECCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCC
PENIDEYLNSGGYQALQLGLTMEPEDIITEIQAANLRGRGGGGFPTGLKWEYAKKGDEKT
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHCCCCCC
EKYVVCNADEGDPGAFMDRSILEGDPHVVIEGMAIAGLAIGANRGIIYVRAEYPLAVKRL
CEEEEEECCCCCCCCHHCCHHHCCCCCEEEECEEEEEEEEECCCCEEEEEECCCHHHHHH
EKAISQAKEYGYLGERVLGSNFSFDIEIKQGAGAFVCGEETALIESVHGNRGMPRPRPPF
HHHHHHHHHCCCHHHHHHCCCCEEEEEEECCCCEEEECCCHHHHHHHCCCCCCCCCCCCC
PAEKGLYDSPTILNNVETLANIPVILNWGAQKYNELGTKDSKGTKVFAITGKVRNTGLCE
CCCCCCCCCCHHHHHHHHHHCCCEEEECCHHHHHHCCCCCCCCCEEEEEECCCCCCCEEE
VPMGIKLKDLIFDIGGGIKDDKEFKAVQIGGPSGGCLPQESLDFPIEYDSLIDAGAMMGS
CCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHEECC
GGLVVMDTSTCMVDLSRFFLNFTQSESCGKCNPCREGTKRLLEILNRIIDGYGENEDLKI
CCEEEEECHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LNRLGNNIINTSLCGLGQSAPNPVLSTIRYFENEYLEHINNKYCRAGVCKNLTTIEVDPE
HHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECHH
KCSGCEICKQICPVDAIKGEKSEVHNIDSKVCIKCKECIDKCPLEAIE
HCCCHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCHHHCC
>Mature Secondary Structure
MRVLVCGGTGCVTSGSQEVKDVLETELEKENIQDYKVIFTGCQGFCEQGPLIRVEQDETF
CEEEEECCCCCCCCCHHHHHHHHHHHHHHCCCCCEEEEEECHHHHHCCCCEEEEECCCEE
YCNVDAEGAKKIVHHHLKNGQLVSELLYENHDGHKVQKREEIDFYQKQERVVINRSGFID
EEECCCHHHHHHHHHHHCCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCEEEEECCCCCC
PENIDEYLNSGGYQALQLGLTMEPEDIITEIQAANLRGRGGGGFPTGLKWEYAKKGDEKT
HHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCCCCCCCEEHHHHCCCCCC
EKYVVCNADEGDPGAFMDRSILEGDPHVVIEGMAIAGLAIGANRGIIYVRAEYPLAVKRL
CEEEEEECCCCCCCCHHCCHHHCCCCCEEEECEEEEEEEEECCCCEEEEEECCCHHHHHH
EKAISQAKEYGYLGERVLGSNFSFDIEIKQGAGAFVCGEETALIESVHGNRGMPRPRPPF
HHHHHHHHHCCCHHHHHHCCCCEEEEEEECCCCEEEECCCHHHHHHHCCCCCCCCCCCCC
PAEKGLYDSPTILNNVETLANIPVILNWGAQKYNELGTKDSKGTKVFAITGKVRNTGLCE
CCCCCCCCCCHHHHHHHHHHCCCEEEECCHHHHHHCCCCCCCCCEEEEEECCCCCCCEEE
VPMGIKLKDLIFDIGGGIKDDKEFKAVQIGGPSGGCLPQESLDFPIEYDSLIDAGAMMGS
CCCCCCHHHHHHHHCCCCCCCCCEEEEEECCCCCCCCCCCCCCCCCCHHHHHHHHHEECC
GGLVVMDTSTCMVDLSRFFLNFTQSESCGKCNPCREGTKRLLEILNRIIDGYGENEDLKI
CCEEEEECHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCHHHH
LNRLGNNIINTSLCGLGQSAPNPVLSTIRYFENEYLEHINNKYCRAGVCKNLTTIEVDPE
HHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCEEEEECHH
KCSGCEICKQICPVDAIKGEKSEVHNIDSKVCIKCKECIDKCPLEAIE
HCCCHHHHHHHCCCCCCCCCHHHHHCCCHHHHHHHHHHHHHCCHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11481432 [H]