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The map label for this gene is nuoE [H]

Identifier: 188585337

GI number: 188585337

Start: 728952

End: 729512

Strand: Direct

Name: nuoE [H]

Synonym: Nther_0702

Alternate gene names: 188585337

Gene position: 728952-729512 (Clockwise)

Preceding gene: 188585336

Following gene: 188585338

Centisome position: 23.03

GC content: 39.39

Gene sequence:

>561_bases
TTGAGTCAAAGTCAGGATACTAAACAAGATACAGCCAAGAGAATTGATACACAAGTGAGACGAGAAGAGCTACCTAAAGA
AGAGTTGCGAGAACTCATTAGAAAACACCGGAAAGATTCAGGAGGTTTAATTCCTCTTTTGCAAACAGTTCAGGAAAGAG
AAGGTTATTTATCCCGTAAAAGGTTGGAATCCATTGCCCGAGAGATGAACCTTTCCCTGGCCAAAATAATGGGAGTAGTT
TCTTTTTATTCACAGTTTCATATCCAGCCTAAAGGCAAAAATATCATACGCGTATGTATGGGAACGGCCTGTCATGTTAA
AGGAGCAGGCCAAGTAATGGAAAAATTCCAAAGAGAACTTTCCATAGAAACAGGTCAAACGACTGAAGATAGGGAGTTTA
GTCTTGAGGCGGTTTCATGTATTGGGGCCTGTGGTCTTGCTCCAGTCTTAACTATTAATCACAATACACACGGAAAAGTT
ACTACTTCTGATGTAAACCATTTGATTAATAGATATGTTAATGGTTCAAATAACGGAAATGAAGGTGGGGAACAAGGATG
A

Upstream 100 bases:

>100_bases
AGTCGATGGGTTATTTTTTACCAAAATTAAACAAGTCTAAAATCTATTCTAACACAAATACTAAACAGTAAAAGTTTCAT
GGTTTAGTGGAGGTGTATAT

Downstream 100 bases:

>100_bases
GAGTTTTGGTTTGTGGTGGTACTGGATGTGTAACATCCGGCTCCCAAGAAGTTAAAGATGTTTTAGAAACAGAATTGGAA
AAAGAGAATATACAAGACTA

Product: NADH dehydrogenase (ubiquinone) 24 kDa subunit

Products: NA

Alternate protein names: NADH dehydrogenase I subunit E; NDH-1 subunit E [H]

Number of amino acids: Translated: 186; Mature: 185

Protein sequence:

>186_residues
MSQSQDTKQDTAKRIDTQVRREELPKEELRELIRKHRKDSGGLIPLLQTVQEREGYLSRKRLESIAREMNLSLAKIMGVV
SFYSQFHIQPKGKNIIRVCMGTACHVKGAGQVMEKFQRELSIETGQTTEDREFSLEAVSCIGACGLAPVLTINHNTHGKV
TTSDVNHLINRYVNGSNNGNEGGEQG

Sequences:

>Translated_186_residues
MSQSQDTKQDTAKRIDTQVRREELPKEELRELIRKHRKDSGGLIPLLQTVQEREGYLSRKRLESIAREMNLSLAKIMGVV
SFYSQFHIQPKGKNIIRVCMGTACHVKGAGQVMEKFQRELSIETGQTTEDREFSLEAVSCIGACGLAPVLTINHNTHGKV
TTSDVNHLINRYVNGSNNGNEGGEQG
>Mature_185_residues
SQSQDTKQDTAKRIDTQVRREELPKEELRELIRKHRKDSGGLIPLLQTVQEREGYLSRKRLESIAREMNLSLAKIMGVVS
FYSQFHIQPKGKNIIRVCMGTACHVKGAGQVMEKFQRELSIETGQTTEDREFSLEAVSCIGACGLAPVLTINHNTHGKVT
TSDVNHLINRYVNGSNNGNEGGEQG

Specific function: NDH-1 shuttles electrons from NADH, via FMN and iron- sulfur (Fe-S) centers, to quinones in the respiratory chain. Couples the redox reaction to proton translocation (for every two electrons transferred, four hydrogen ions are translocated across the cyto

COG id: COG1905

COG function: function code C; NADH:ubiquinone oxidoreductase 24 kD subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the complex I 24 kDa subunit family [H]

Homologues:

Organism=Homo sapiens, GI222080062, Length=131, Percent_Identity=30.5343511450382, Blast_Score=96, Evalue=3e-20,
Organism=Escherichia coli, GI1788621, Length=147, Percent_Identity=29.2517006802721, Blast_Score=95, Evalue=2e-21,
Organism=Caenorhabditis elegans, GI17561328, Length=131, Percent_Identity=35.1145038167939, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI18859877, Length=130, Percent_Identity=33.8461538461538, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21355353, Length=150, Percent_Identity=30, Blast_Score=100, Evalue=5e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002023
- InterPro:   IPR012336
- InterPro:   IPR012335 [H]

Pfam domain/function: PF01257 Complex1_24kDa [H]

EC number: =1.6.99.5 [H]

Molecular weight: Translated: 20794; Mature: 20663

Theoretical pI: Translated: 8.65; Mature: 8.65

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
4.8 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQSQDTKQDTAKRIDTQVRREELPKEELRELIRKHRKDSGGLIPLLQTVQEREGYLSRK
CCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
RLESIAREMNLSLAKIMGVVSFYSQFHIQPKGKNIIRVCMGTACHVKGAGQVMEKFQREL
HHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHCCCCHHHHHHHHHHHH
SIETGQTTEDREFSLEAVSCIGACGLAPVLTINHNTHGKVTTSDVNHLINRYVNGSNNGN
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEHHHHHHHHHHHCCCCCCCC
EGGEQG
CCCCCC
>Mature Secondary Structure 
SQSQDTKQDTAKRIDTQVRREELPKEELRELIRKHRKDSGGLIPLLQTVQEREGYLSRK
CCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHHHH
RLESIAREMNLSLAKIMGVVSFYSQFHIQPKGKNIIRVCMGTACHVKGAGQVMEKFQREL
HHHHHHHHHCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHCCHHCCCCHHHHHHHHHHHH
SIETGQTTEDREFSLEAVSCIGACGLAPVLTINHNTHGKVTTSDVNHLINRYVNGSNNGN
CCCCCCCCCCHHHHHHHHHHHHHCCCCCEEEECCCCCCCEEHHHHHHHHHHHCCCCCCCC
EGGEQG
CCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12522265 [H]