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The map label for this gene is metF [H]

Identifier: 188585317

GI number: 188585317

Start: 706055

End: 706972

Strand: Direct

Name: metF [H]

Synonym: Nther_0681

Alternate gene names: 188585317

Gene position: 706055-706972 (Clockwise)

Preceding gene: 188585316

Following gene: 188585318

Centisome position: 22.3

GC content: 35.51

Gene sequence:

>918_bases
ATGAAGATATCCGAAATTTTTTCGCAAAGTAACGGACCTGTTGTCTCCTTTGAGATATTTCCGCCCAAAAGGAAAAAAAA
TCAATCAAAAGAGGATTTTGATTCTTTATTAAAAACAATTGATAATTTAGCTCGGTTAAAACCTGATTTTATAAGTGTGA
CTTACGGAGCTGGCGGTACCAATAGTGATGGTAGCTTAGAAATTTCCGAATACGTTAAAAACAAAGGGATTACGTCTTTA
CCTCACTTTACATCCATCGGTTATCCCGTTGAAGAGATTAATTCGTATTTAGGTACAATTTACAACAAGGGTTTTGAAAA
TATTTTGGCTTTAAGAGGAGATCTGCCGAAAAGTCCTTCAAAAGCAGACTTGGTTTGGAGAGATTTTTTACATGCAACTG
ATTTGATCAAGGTTATCTCAAGGGATTTTCCGAATTTCTGCATAGGGGGAGCCGCTTACCCCGAAGGTCATCAAGAATCT
AGTTATGCAGGGCAGGATATCGAGATTATGAAAATCAAAGAAAAAATGGGTGTAAAGTTTTTCTTAACCCAACTGTTTTT
CAATAACGATAATTTCTTTAAATTTATGGAAAATGTCAGGAATAATGGGGTGGAATCTCCAGTTTCTGCAGGTGTCATGC
CTTTGATTAGTCCTAAATACGTGGACAAAATCATTGAATTATCAGGTGTAAAATTACCAAAAGAATTAGAAAATGCTTTG
GAAAATTACAGGGATGATGAAGTAGAATTTAGAAAAAGATCCTTAGATTATTGTGTTAGACAGGTGAACGATTTATTTGA
AAGAGGTGTGGATGGAGTGCATCTCTATACGATGAATCGCCATGAAGCTGTTGAAGAAATTCTTGACCAAGTTGATTCAA
ATGTAAACAGGGAGGTGGAACTATCAAAACAGACTTGA

Upstream 100 bases:

>100_bases
GAAAATAATGGCATTTTAAAGCCAAGAAAAAGTACTATTTGTCTAGTCGGTTTTAATGATATAGCAGTCAAGAATACCAA
AAAGGGAAGGTGGTTTTTGT

Downstream 100 bases:

>100_bases
AACTAAAAGATCAACTAGTTAATAAAACTTTGGAGAAGTTACTCTTTCTCGATGGCTCCTATGGTACAGAACTACAAAGA
AGAGGTTTTGAAGGCAATGC

Product: Methylenetetrahydrofolate reductase (NAD(P)H)

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MKISEIFSQSNGPVVSFEIFPPKRKKNQSKEDFDSLLKTIDNLARLKPDFISVTYGAGGTNSDGSLEISEYVKNKGITSL
PHFTSIGYPVEEINSYLGTIYNKGFENILALRGDLPKSPSKADLVWRDFLHATDLIKVISRDFPNFCIGGAAYPEGHQES
SYAGQDIEIMKIKEKMGVKFFLTQLFFNNDNFFKFMENVRNNGVESPVSAGVMPLISPKYVDKIIELSGVKLPKELENAL
ENYRDDEVEFRKRSLDYCVRQVNDLFERGVDGVHLYTMNRHEAVEEILDQVDSNVNREVELSKQT

Sequences:

>Translated_305_residues
MKISEIFSQSNGPVVSFEIFPPKRKKNQSKEDFDSLLKTIDNLARLKPDFISVTYGAGGTNSDGSLEISEYVKNKGITSL
PHFTSIGYPVEEINSYLGTIYNKGFENILALRGDLPKSPSKADLVWRDFLHATDLIKVISRDFPNFCIGGAAYPEGHQES
SYAGQDIEIMKIKEKMGVKFFLTQLFFNNDNFFKFMENVRNNGVESPVSAGVMPLISPKYVDKIIELSGVKLPKELENAL
ENYRDDEVEFRKRSLDYCVRQVNDLFERGVDGVHLYTMNRHEAVEEILDQVDSNVNREVELSKQT
>Mature_305_residues
MKISEIFSQSNGPVVSFEIFPPKRKKNQSKEDFDSLLKTIDNLARLKPDFISVTYGAGGTNSDGSLEISEYVKNKGITSL
PHFTSIGYPVEEINSYLGTIYNKGFENILALRGDLPKSPSKADLVWRDFLHATDLIKVISRDFPNFCIGGAAYPEGHQES
SYAGQDIEIMKIKEKMGVKFFLTQLFFNNDNFFKFMENVRNNGVESPVSAGVMPLISPKYVDKIIELSGVKLPKELENAL
ENYRDDEVEFRKRSLDYCVRQVNDLFERGVDGVHLYTMNRHEAVEEILDQVDSNVNREVELSKQT

Specific function: Methionine biosynthesis. [C]

COG id: COG0685

COG function: function code E; 5,10-methylenetetrahydrofolate reductase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methylenetetrahydrofolate reductase family [H]

Homologues:

Organism=Homo sapiens, GI87240000, Length=289, Percent_Identity=30.4498269896194, Blast_Score=137, Evalue=1e-32,
Organism=Escherichia coli, GI1790377, Length=282, Percent_Identity=31.5602836879433, Blast_Score=143, Evalue=2e-35,
Organism=Caenorhabditis elegans, GI25147436, Length=282, Percent_Identity=29.0780141843972, Blast_Score=130, Evalue=9e-31,
Organism=Caenorhabditis elegans, GI25147441, Length=282, Percent_Identity=29.0780141843972, Blast_Score=130, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6325234, Length=310, Percent_Identity=29.0322580645161, Blast_Score=133, Evalue=4e-32,
Organism=Saccharomyces cerevisiae, GI6321313, Length=303, Percent_Identity=30.3630363036304, Blast_Score=130, Evalue=2e-31,
Organism=Drosophila melanogaster, GI24662658, Length=244, Percent_Identity=23.3606557377049, Blast_Score=77, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003171
- InterPro:   IPR004620 [H]

Pfam domain/function: PF02219 MTHFR [H]

EC number: =1.5.1.20 [H]

Molecular weight: Translated: 34618; Mature: 34618

Theoretical pI: Translated: 5.03; Mature: 5.03

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKISEIFSQSNGPVVSFEIFPPKRKKNQSKEDFDSLLKTIDNLARLKPDFISVTYGAGGT
CCHHHHHCCCCCCEEEEEECCCHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
NSDGSLEISEYVKNKGITSLPHFTSIGYPVEEINSYLGTIYNKGFENILALRGDLPKSPS
CCCCCEEHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KADLVWRDFLHATDLIKVISRDFPNFCIGGAAYPEGHQESSYAGQDIEIMKIKEKMGVKF
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHH
FLTQLFFNNDNFFKFMENVRNNGVESPVSAGVMPLISPKYVDKIIELSGVKLPKELENAL
HHHHHHHCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHH
ENYRDDEVEFRKRSLDYCVRQVNDLFERGVDGVHLYTMNRHEAVEEILDQVDSNVNREVE
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEE
LSKQT
ECCCC
>Mature Secondary Structure
MKISEIFSQSNGPVVSFEIFPPKRKKNQSKEDFDSLLKTIDNLARLKPDFISVTYGAGGT
CCHHHHHCCCCCCEEEEEECCCHHCCCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCCCC
NSDGSLEISEYVKNKGITSLPHFTSIGYPVEEINSYLGTIYNKGFENILALRGDLPKSPS
CCCCCEEHHHHHHCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
KADLVWRDFLHATDLIKVISRDFPNFCIGGAAYPEGHQESSYAGQDIEIMKIKEKMGVKF
HHHHHHHHHHHHHHHHHHHHHCCCCCEECCCCCCCCCCCCCCCCCCCCEEEHHHHHHHHH
FLTQLFFNNDNFFKFMENVRNNGVESPVSAGVMPLISPKYVDKIIELSGVKLPKELENAL
HHHHHHHCCCHHHHHHHHHHHCCCCCCHHCCCCCCCCHHHHHHHHHHCCCCCCHHHHHHH
ENYRDDEVEFRKRSLDYCVRQVNDLFERGVDGVHLYTMNRHEAVEEILDQVDSNVNREVE
HHCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHCCCCCCEEE
LSKQT
ECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9537320 [H]