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The map label for this gene is metH [H]

Identifier: 188585318

GI number: 188585318

Start: 706969

End: 709350

Strand: Direct

Name: metH [H]

Synonym: Nther_0682

Alternate gene names: 188585318

Gene position: 706969-709350 (Clockwise)

Preceding gene: 188585317

Following gene: 188585319

Centisome position: 22.33

GC content: 36.73

Gene sequence:

>2382_bases
TTGAAACTAAAAGATCAACTAGTTAATAAAACTTTGGAGAAGTTACTCTTTCTCGATGGCTCCTATGGTACAGAACTACA
AAGAAGAGGTTTTGAAGGCAATGCGGAAATTTTAAATTTACAAGATCAGGATTTGGTAAAAAATGTGCATTATGATTATG
CCTCAGCTGGTGCTGATTTAATTTTAACAAACACTTTTGGTGGTAATAGATTAAAATTGAAAGATGCCGGATATGAGGAT
AAAGTTGCAGAAATTAATAGCAGAGGTGTTGAGCTGGCCAAGGAGTCAGCTGAAAATATTGATAGAGATGTTTTGGTAGT
AGGTGATATATCATCTACCGGTCGATTTATCGAGCCCGTAGGTGATTTGTCCTTTACAAAAGCTCAACAAGTATTCCAGG
AACAAGGTCGTTATTTGATAGAAAGTGGAGTTGATCTAATAAAAATTGAAACAATGACGGATATTAAAGAGCTAAAAGCT
GCTATATTAGGGATACGTTCAATAGATGAGGATTTACCCCTGATAGTTAGTATGAGTTTTGAACAAGATGGTAAAACAAT
TACTGGTACATCTGTACCATCTTACGTCACTTTAATGAATGATCTACCTGTGGATATTATTGGTTTGAACTGTGGAGTCA
AATCGAAAGATATGTTGGATCTTGTCAAACAGGCTTCTTACTATTCACTCAAACCACTTAGCATTTCTCCTAATGCAGGA
GATCCGGACTTAGATCTGAGTTATAGTGAAGATCCTCAAGAATTTTCATTTTATCTTGATGCCATTTCTGATTTAGATAT
GGTAGCTATTGTAGGAGGGTGTTGTGGTACCACACCCGAATATGTTGACAATTATTGTTCTAAATTAAAAGGCAAGGAAA
AGTCCCGCAAGTTATTGCAACAATCTCAAATCCACAAAGATATACCAACTTTGATATCATCAAGAACTTATCAACTTGAT
TTAGACAATAATTTTGTCAAAATTGGTGAACGAATTAATCCAGCCTCCAAGAAAAACTTTCAAGAAGAAATTAATAATAA
AGATTTTTCAAGGATTTATGATGAAGCCTTTAGCCAGATGAATGAAGGTAGTGATGTGATTGATCTCAATTTAGGTGTGG
AAGAGGACGTGGAACTAGAAGATGTGGAGACAATTGTCAACGGCTTGGATTCCCTTGGATGTAAACCATTATCTTTGGAT
ATTCAGTCATCAGTACTCTTGGAAAAGGCACTACAGGTTTATCCGGGTAGGCCGATAATTAATTCGTCATCCTGTGACAA
AGAAGACTTGGATAATGCCATTGACTTAATGAAAAAATACGGCGGAGTTTTAGTCTTGCTTGCTATGTCAGGTACTCCTA
AAAAAACAATTCAAAAGCGATTGGATGAATTGAAATATGGCCTAGAATATTTAGAATCAAAAGGTATTGATGAAAGCAGG
ATTATGGTAGATCCCTTGGTTTTACCGGTGGGTACTGAACATGACCCAAAAACTACTTTGGAGCTGATCAAAATTATTTC
CCAGGAAATGGAGTGCAAGACAACTCTAGGCTTATCAAATTTAAGTCATGGATTGCCCGATAGACCCCAACTGAACTCTG
CCTTTGTTTCCATGGCAATTTATAATGGATTAGACAGCGCTATTATGGATACTTCAGATCAACTGGTAACCCAGACTGTG
GAAAAATCACTGATGTTAATGGGTAAAGAAACAATTTGGGAAGATGTAGCAATCGATACTGAAGATCAGATGGTAAAAGA
CATTTTGACAGGCAATTCCGACAAAGTATTAACACAGGTGAAATCTTATTTAAAGGATATGACTCCTTTGGAAGTGAGTC
AGCAACTGCTGGGAAAAGCTATGAAAGAGATTGGTGATTTATACAACGATAAAAGTATCTACTTACCCCAGTTGTTATTG
TCAGCAGAAACAGTGCAACCTTCCTTTGACTACTTAAATAAACTTGTAGGGGAGCACCAAGAAGATAGTAAAGGAAAAAT
TATGTTAGCTACCGTGGAAGGTGATGTACATGACATAGGCAAAAAAATTGTAGGAACTATATTGAAAAGCGGAGGATTTG
AGATATATGACTTAGGTGTTGATCTTCCAGCTAATGAAATTGTAGCAGAAGTCAAAGATATCAAACCAGATATCCTGGGT
TTATCGGCCATGATGACTAGCACAATTAAAAAAATTGCAGAAGTCACAGAACAGCTTCAAGCTGAAGGAATTGATGTAAA
GGTGATCGGAGGAGGCGCTTCTCTAAATCCTGAACTAGCCCAAAAGTTTGGTTGTCATGCTTATGCCAAGGATGCACATG
AAGCGTTAACCAAATGTGAACAATTAATGACTGGTGAAAAAGCTTTGTCTAATGAGAGGTGA

Upstream 100 bases:

>100_bases
GAGTGCATCTCTATACGATGAATCGCCATGAAGCTGTTGAAGAAATTCTTGACCAAGTTGATTCAAATGTAAACAGGGAG
GTGGAACTATCAAAACAGAC

Downstream 100 bases:

>100_bases
GGTGAAGAGATGGCTAATTTAACCTCACGTGATAGAGATGTATTGAAAGATTTACATCACTTTCGGGGATTGTTCTCAGA
AGCTGTCAAAAAAAGACATT

Product: homocysteine S-methyltransferase

Products: NA

Alternate protein names: 5-methyltetrahydrofolate--homocysteine methyltransferase; Methionine synthase, vitamin-B12 dependent; MS [H]

Number of amino acids: Translated: 793; Mature: 793

Protein sequence:

>793_residues
MKLKDQLVNKTLEKLLFLDGSYGTELQRRGFEGNAEILNLQDQDLVKNVHYDYASAGADLILTNTFGGNRLKLKDAGYED
KVAEINSRGVELAKESAENIDRDVLVVGDISSTGRFIEPVGDLSFTKAQQVFQEQGRYLIESGVDLIKIETMTDIKELKA
AILGIRSIDEDLPLIVSMSFEQDGKTITGTSVPSYVTLMNDLPVDIIGLNCGVKSKDMLDLVKQASYYSLKPLSISPNAG
DPDLDLSYSEDPQEFSFYLDAISDLDMVAIVGGCCGTTPEYVDNYCSKLKGKEKSRKLLQQSQIHKDIPTLISSRTYQLD
LDNNFVKIGERINPASKKNFQEEINNKDFSRIYDEAFSQMNEGSDVIDLNLGVEEDVELEDVETIVNGLDSLGCKPLSLD
IQSSVLLEKALQVYPGRPIINSSSCDKEDLDNAIDLMKKYGGVLVLLAMSGTPKKTIQKRLDELKYGLEYLESKGIDESR
IMVDPLVLPVGTEHDPKTTLELIKIISQEMECKTTLGLSNLSHGLPDRPQLNSAFVSMAIYNGLDSAIMDTSDQLVTQTV
EKSLMLMGKETIWEDVAIDTEDQMVKDILTGNSDKVLTQVKSYLKDMTPLEVSQQLLGKAMKEIGDLYNDKSIYLPQLLL
SAETVQPSFDYLNKLVGEHQEDSKGKIMLATVEGDVHDIGKKIVGTILKSGGFEIYDLGVDLPANEIVAEVKDIKPDILG
LSAMMTSTIKKIAEVTEQLQAEGIDVKVIGGGASLNPELAQKFGCHAYAKDAHEALTKCEQLMTGEKALSNER

Sequences:

>Translated_793_residues
MKLKDQLVNKTLEKLLFLDGSYGTELQRRGFEGNAEILNLQDQDLVKNVHYDYASAGADLILTNTFGGNRLKLKDAGYED
KVAEINSRGVELAKESAENIDRDVLVVGDISSTGRFIEPVGDLSFTKAQQVFQEQGRYLIESGVDLIKIETMTDIKELKA
AILGIRSIDEDLPLIVSMSFEQDGKTITGTSVPSYVTLMNDLPVDIIGLNCGVKSKDMLDLVKQASYYSLKPLSISPNAG
DPDLDLSYSEDPQEFSFYLDAISDLDMVAIVGGCCGTTPEYVDNYCSKLKGKEKSRKLLQQSQIHKDIPTLISSRTYQLD
LDNNFVKIGERINPASKKNFQEEINNKDFSRIYDEAFSQMNEGSDVIDLNLGVEEDVELEDVETIVNGLDSLGCKPLSLD
IQSSVLLEKALQVYPGRPIINSSSCDKEDLDNAIDLMKKYGGVLVLLAMSGTPKKTIQKRLDELKYGLEYLESKGIDESR
IMVDPLVLPVGTEHDPKTTLELIKIISQEMECKTTLGLSNLSHGLPDRPQLNSAFVSMAIYNGLDSAIMDTSDQLVTQTV
EKSLMLMGKETIWEDVAIDTEDQMVKDILTGNSDKVLTQVKSYLKDMTPLEVSQQLLGKAMKEIGDLYNDKSIYLPQLLL
SAETVQPSFDYLNKLVGEHQEDSKGKIMLATVEGDVHDIGKKIVGTILKSGGFEIYDLGVDLPANEIVAEVKDIKPDILG
LSAMMTSTIKKIAEVTEQLQAEGIDVKVIGGGASLNPELAQKFGCHAYAKDAHEALTKCEQLMTGEKALSNER
>Mature_793_residues
MKLKDQLVNKTLEKLLFLDGSYGTELQRRGFEGNAEILNLQDQDLVKNVHYDYASAGADLILTNTFGGNRLKLKDAGYED
KVAEINSRGVELAKESAENIDRDVLVVGDISSTGRFIEPVGDLSFTKAQQVFQEQGRYLIESGVDLIKIETMTDIKELKA
AILGIRSIDEDLPLIVSMSFEQDGKTITGTSVPSYVTLMNDLPVDIIGLNCGVKSKDMLDLVKQASYYSLKPLSISPNAG
DPDLDLSYSEDPQEFSFYLDAISDLDMVAIVGGCCGTTPEYVDNYCSKLKGKEKSRKLLQQSQIHKDIPTLISSRTYQLD
LDNNFVKIGERINPASKKNFQEEINNKDFSRIYDEAFSQMNEGSDVIDLNLGVEEDVELEDVETIVNGLDSLGCKPLSLD
IQSSVLLEKALQVYPGRPIINSSSCDKEDLDNAIDLMKKYGGVLVLLAMSGTPKKTIQKRLDELKYGLEYLESKGIDESR
IMVDPLVLPVGTEHDPKTTLELIKIISQEMECKTTLGLSNLSHGLPDRPQLNSAFVSMAIYNGLDSAIMDTSDQLVTQTV
EKSLMLMGKETIWEDVAIDTEDQMVKDILTGNSDKVLTQVKSYLKDMTPLEVSQQLLGKAMKEIGDLYNDKSIYLPQLLL
SAETVQPSFDYLNKLVGEHQEDSKGKIMLATVEGDVHDIGKKIVGTILKSGGFEIYDLGVDLPANEIVAEVKDIKPDILG
LSAMMTSTIKKIAEVTEQLQAEGIDVKVIGGGASLNPELAQKFGCHAYAKDAHEALTKCEQLMTGEKALSNER

Specific function: Catalyzes the transfer of a methyl group from methyl- cobalamin to homocysteine, yielding enzyme-bound cob(I)alamin and methionine. Subsequently, remethylates the cofactor using methyltetrahydrofolate [H]

COG id: COG1410

COG function: function code E; Methionine synthase I, cobalamin-binding domain

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 pterin-binding domain [H]

Homologues:

Organism=Homo sapiens, GI169790923, Length=889, Percent_Identity=25.6467941507312, Blast_Score=267, Evalue=2e-71,
Organism=Escherichia coli, GI1790450, Length=847, Percent_Identity=26.3282172373081, Blast_Score=262, Evalue=7e-71,
Organism=Escherichia coli, GI1786456, Length=291, Percent_Identity=25.085910652921, Blast_Score=72, Evalue=1e-13,
Organism=Caenorhabditis elegans, GI17535405, Length=859, Percent_Identity=24.4470314318976, Blast_Score=254, Evalue=1e-67,
Organism=Saccharomyces cerevisiae, GI6324982, Length=316, Percent_Identity=24.6835443037975, Blast_Score=67, Evalue=8e-12,
Organism=Saccharomyces cerevisiae, GI6322966, Length=313, Percent_Identity=24.6006389776358, Blast_Score=66, Evalue=2e-11,

Paralogues:

None

Copy number: 513 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 1320 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006158
- InterPro:   IPR011005
- InterPro:   IPR003759
- InterPro:   IPR011822
- InterPro:   IPR000489
- InterPro:   IPR003726
- InterPro:   IPR004223 [H]

Pfam domain/function: PF02310 B12-binding; PF02607 B12-binding_2; PF02965 Met_synt_B12; PF00809 Pterin_bind; PF02574 S-methyl_trans [H]

EC number: =2.1.1.13 [H]

Molecular weight: Translated: 87410; Mature: 87410

Theoretical pI: Translated: 4.34; Mature: 4.34

Prosite motif: PS50970 HCY ; PS50972 PTERIN_BINDING ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.8 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLKDQLVNKTLEKLLFLDGSYGTELQRRGFEGNAEILNLQDQDLVKNVHYDYASAGADL
CCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEECCCHHHHHHHHCCHHHCCCCCE
ILTNTFGGNRLKLKDAGYEDKVAEINSRGVELAKESAENIDRDVLVVGDISSTGRFIEPV
EEEECCCCCEEEEECCCCCHHHHHHHHCHHHHHHHHHHCCCCCEEEEECCCCCCCHHCCC
GDLSFTKAQQVFQEQGRYLIESGVDLIKIETMTDIKELKAAILGIRSIDEDLPLIVSMSF
CCCHHHHHHHHHHHCCCEEHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
EQDGKTITGTSVPSYVTLMNDLPVDIIGLNCGVKSKDMLDLVKQASYYSLKPLSISPNAG
CCCCCEEECCCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEECCCEECCCCC
DPDLDLSYSEDPQEFSFYLDAISDLDMVAIVGGCCGTTPEYVDNYCSKLKGKEKSRKLLQ
CCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
QSQIHKDIPTLISSRTYQLDLDNNFVKIGERINPASKKNFQEEINNKDFSRIYDEAFSQM
HHHHHHHHHHHHCCCEEEEECCCCEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
NEGSDVIDLNLGVEEDVELEDVETIVNGLDSLGCKPLSLDIQSSVLLEKALQVYPGRPII
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHCCCCCCC
NSSSCDKEDLDNAIDLMKKYGGVLVLLAMSGTPKKTIQKRLDELKYGLEYLESKGIDESR
CCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IMVDPLVLPVGTEHDPKTTLELIKIISQEMECKTTLGLSNLSHGLPDRPQLNSAFVSMAI
EEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHH
YNGLDSAIMDTSDQLVTQTVEKSLMLMGKETIWEDVAIDTEDQMVKDILTGNSDKVLTQV
HHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHH
KSYLKDMTPLEVSQQLLGKAMKEIGDLYNDKSIYLPQLLLSAETVQPSFDYLNKLVGEHQ
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCC
EDSKGKIMLATVEGDVHDIGKKIVGTILKSGGFEIYDLGVDLPANEIVAEVKDIKPDILG
CCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCHHHHH
LSAMMTSTIKKIAEVTEQLQAEGIDVKVIGGGASLNPELAQKFGCHAYAKDAHEALTKCE
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHH
QLMTGEKALSNER
HHHHHHHHHCCCC
>Mature Secondary Structure
MKLKDQLVNKTLEKLLFLDGSYGTELQRRGFEGNAEILNLQDQDLVKNVHYDYASAGADL
CCCHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCEEECCCHHHHHHHHCCHHHCCCCCE
ILTNTFGGNRLKLKDAGYEDKVAEINSRGVELAKESAENIDRDVLVVGDISSTGRFIEPV
EEEECCCCCEEEEECCCCCHHHHHHHHCHHHHHHHHHHCCCCCEEEEECCCCCCCHHCCC
GDLSFTKAQQVFQEQGRYLIESGVDLIKIETMTDIKELKAAILGIRSIDEDLPLIVSMSF
CCCHHHHHHHHHHHCCCEEHHCCCCEEEEEHHHHHHHHHHHHHHHHHCCCCCCEEEEEEE
EQDGKTITGTSVPSYVTLMNDLPVDIIGLNCGVKSKDMLDLVKQASYYSLKPLSISPNAG
CCCCCEEECCCCCHHHHHHCCCCEEEEEECCCCCCHHHHHHHHHCCCCEECCCEECCCCC
DPDLDLSYSEDPQEFSFYLDAISDLDMVAIVGGCCGTTPEYVDNYCSKLKGKEKSRKLLQ
CCCCCCCCCCCHHHHHHHHHHHCCCHHHHHHCCCCCCCHHHHHHHHHHHCCCHHHHHHHH
QSQIHKDIPTLISSRTYQLDLDNNFVKIGERINPASKKNFQEEINNKDFSRIYDEAFSQM
HHHHHHHHHHHHCCCEEEEECCCCEEECCCCCCCHHHHHHHHHHCCCHHHHHHHHHHHHH
NEGSDVIDLNLGVEEDVELEDVETIVNGLDSLGCKPLSLDIQSSVLLEKALQVYPGRPII
CCCCCEEEEECCCCCCCCHHHHHHHHHHHHHCCCCEEEECHHHHHHHHHHHHHCCCCCCC
NSSSCDKEDLDNAIDLMKKYGGVLVLLAMSGTPKKTIQKRLDELKYGLEYLESKGIDESR
CCCCCCHHHHHHHHHHHHHCCCEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHCCCCCCC
IMVDPLVLPVGTEHDPKTTLELIKIISQEMECKTTLGLSNLSHGLPDRPQLNSAFVSMAI
EEECCEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHCHHHHHCCCCCCCCHHHHHHHHHH
YNGLDSAIMDTSDQLVTQTVEKSLMLMGKETIWEDVAIDTEDQMVKDILTGNSDKVLTQV
HHCCHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCHHHHHHH
KSYLKDMTPLEVSQQLLGKAMKEIGDLYNDKSIYLPQLLLSAETVQPSFDYLNKLVGEHQ
HHHHHCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCCCHHHHHHHHHCCCC
EDSKGKIMLATVEGDVHDIGKKIVGTILKSGGFEIYDLGVDLPANEIVAEVKDIKPDILG
CCCCCCEEEEEECCCHHHHHHHHHHHHHHCCCCEEEEECCCCCHHHHHHHHHHCCHHHHH
LSAMMTSTIKKIAEVTEQLQAEGIDVKVIGGGASLNPELAQKFGCHAYAKDAHEALTKCE
HHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCCCCHHHHHHHCCHHHHHHHHHHHHHHH
QLMTGEKALSNER
HHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9634230 [H]