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The map label for this gene is cls [H]

Identifier: 188585276

GI number: 188585276

Start: 660217

End: 661635

Strand: Reverse

Name: cls [H]

Synonym: Nther_0638

Alternate gene names: 188585276

Gene position: 661635-660217 (Counterclockwise)

Preceding gene: 188585286

Following gene: 188585274

Centisome position: 20.9

GC content: 34.53

Gene sequence:

>1419_bases
GTGACCTTAATACCTTTAATATTATCAACAATCACACTATTAAATATTGTTGTAGTAATAACTTTAATCTTTTTCGAGAG
AAAAAATCCTACTGCCACTTGGGCATGGGTTTTAATCCTGGTTTTATTTCCTATACTCGGTTTAATTCTTTATCTGTTCA
TTGGTCTTACTCCGCGAAAAAGAAAAATTTTCGATCGCAAAACTGAAAAAGATCTAATTAAAAATAAGCAATTAATTGAA
GGCTCACCCAATTATCAAGGTAAAAGCTTGCCTCATCAGGCAAACAATATCTTTCAAATTGATTTTGGTAAAGAAGAAAT
CGTCTACACCGATAACAATGATGTTCAGATTTATACCCATGGAAAAGAAAAATTCAAAGAATTATTTAATGTCATGGAAA
CAGCCGAACATCATATCCACATTAGCTATTATATCATTCGTAACGACGAATTGGGAAAAGCTATAGTAAACAAATTAACC
AAAAAAGCTCGTCAAGGCGTCACAGTTAGGGTATTGTATGATAGTGTGGGTTGTAGAAAACTTCCAAAAGATTTCTTTTC
CGAATTGGAAGAAGCAGGCGGTCTTGTAACACGATTTTTTCCTTTTATCTTAGATATTAATTACAGAAATCACAGAAAAA
TCGCTGTTATAGATGGCAAAACAGCTTTTGTGGGCGGTACAAATATAGGTAAAGAATATCTTGGTATTTCCCAGCGTTTT
GATGATTGGCGGGATACCCACTTAAAAGTTACTGGCGATGCCGCTAAGTCCCTTCAAGTTAGGTTTTTACTAGATTGGAT
TTTCGCAACGGAAGAAGACGAAGATATCATGATGAATCGAGAATTTTTCCCAGAGATTTCTGATCAAGGTAATTCAGGTA
TTCAAATAGTAGCAAGTGGACCTGACTCCCCTCACGAAGAGATTAAAGCTTTTTTACTAAAAATGATCTACTCTGCTAAG
GAATCAATTTACTTGCAAACACCGTATTTTATTCCAGATGAAAGCATTCTGGAAGCTTTGCAAATAGCAGCCTATTCCGG
TGTTGATGTTAGAATTGCTATCCCTGATAAGCCAGATCATCCCTTTGTATTTTGGGCCAATAGAGCTTATCTAGGCCTTA
TGCTAGAAACTGGCGCTAGGGGATTTATTTATAAACATGGTTTTCTACACAGCAAGTTAATTATAGTTGACGGAAAAATT
GCATCTGTAGGAACTGCTAATATGGATGTAAGAAGTTTTAAATTAAATTTTGAAGTCAATGCTTTTATCTATGATCCACA
AATGGCCCAACGACTTAATGAAATTTTTCTAAGAGATCTAGATAATTCTTGCGAATTAACGGAAGACCAGTATAATCAAA
GGGGAATATCTTCCAAATTTAAAGAATCTATTTCCCGATTATTATCACCAATTCTATGA

Upstream 100 bases:

>100_bases
GATTCCTACAAGTTAAAATCATCAAAACAACTCCTTAAACTAAATTTAAAACCACCTTATCCGGATCAATTCATGTATTT
TCCTAAAGGATGGTGAAAGC

Downstream 100 bases:

>100_bases
AAACAGAAGAAACTTAATTCCTAAGCCCCTCTTTTTTGTTTGTGATCATAAAAGAGGGGTGTTTTTTAACAGCAACTTCC
ACCACTACAAGTTCCACAAG

Product: phospholipase D/Transphosphatidylase

Products: NA

Alternate protein names: CL synthase [H]

Number of amino acids: Translated: 472; Mature: 471

Protein sequence:

>472_residues
MTLIPLILSTITLLNIVVVITLIFFERKNPTATWAWVLILVLFPILGLILYLFIGLTPRKRKIFDRKTEKDLIKNKQLIE
GSPNYQGKSLPHQANNIFQIDFGKEEIVYTDNNDVQIYTHGKEKFKELFNVMETAEHHIHISYYIIRNDELGKAIVNKLT
KKARQGVTVRVLYDSVGCRKLPKDFFSELEEAGGLVTRFFPFILDINYRNHRKIAVIDGKTAFVGGTNIGKEYLGISQRF
DDWRDTHLKVTGDAAKSLQVRFLLDWIFATEEDEDIMMNREFFPEISDQGNSGIQIVASGPDSPHEEIKAFLLKMIYSAK
ESIYLQTPYFIPDESILEALQIAAYSGVDVRIAIPDKPDHPFVFWANRAYLGLMLETGARGFIYKHGFLHSKLIIVDGKI
ASVGTANMDVRSFKLNFEVNAFIYDPQMAQRLNEIFLRDLDNSCELTEDQYNQRGISSKFKESISRLLSPIL

Sequences:

>Translated_472_residues
MTLIPLILSTITLLNIVVVITLIFFERKNPTATWAWVLILVLFPILGLILYLFIGLTPRKRKIFDRKTEKDLIKNKQLIE
GSPNYQGKSLPHQANNIFQIDFGKEEIVYTDNNDVQIYTHGKEKFKELFNVMETAEHHIHISYYIIRNDELGKAIVNKLT
KKARQGVTVRVLYDSVGCRKLPKDFFSELEEAGGLVTRFFPFILDINYRNHRKIAVIDGKTAFVGGTNIGKEYLGISQRF
DDWRDTHLKVTGDAAKSLQVRFLLDWIFATEEDEDIMMNREFFPEISDQGNSGIQIVASGPDSPHEEIKAFLLKMIYSAK
ESIYLQTPYFIPDESILEALQIAAYSGVDVRIAIPDKPDHPFVFWANRAYLGLMLETGARGFIYKHGFLHSKLIIVDGKI
ASVGTANMDVRSFKLNFEVNAFIYDPQMAQRLNEIFLRDLDNSCELTEDQYNQRGISSKFKESISRLLSPIL
>Mature_471_residues
TLIPLILSTITLLNIVVVITLIFFERKNPTATWAWVLILVLFPILGLILYLFIGLTPRKRKIFDRKTEKDLIKNKQLIEG
SPNYQGKSLPHQANNIFQIDFGKEEIVYTDNNDVQIYTHGKEKFKELFNVMETAEHHIHISYYIIRNDELGKAIVNKLTK
KARQGVTVRVLYDSVGCRKLPKDFFSELEEAGGLVTRFFPFILDINYRNHRKIAVIDGKTAFVGGTNIGKEYLGISQRFD
DWRDTHLKVTGDAAKSLQVRFLLDWIFATEEDEDIMMNREFFPEISDQGNSGIQIVASGPDSPHEEIKAFLLKMIYSAKE
SIYLQTPYFIPDESILEALQIAAYSGVDVRIAIPDKPDHPFVFWANRAYLGLMLETGARGFIYKHGFLHSKLIIVDGKIA
SVGTANMDVRSFKLNFEVNAFIYDPQMAQRLNEIFLRDLDNSCELTEDQYNQRGISSKFKESISRLLSPIL

Specific function: Catalyzes the reversible phosphatidyl group transfer from one phosphatidylglycerol molecule to another to form cardiolipin (CL) (diphosphatidylglycerol) and glycerol [H]

COG id: COG1502

COG function: function code I; Phosphatidylserine/phosphatidylglycerophosphate/cardiolipin synthases and related enzymes

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 PLD phosphodiesterase domains [H]

Homologues:

Organism=Escherichia coli, GI1787502, Length=493, Percent_Identity=26.9776876267748, Blast_Score=192, Evalue=4e-50,
Organism=Escherichia coli, GI1787007, Length=347, Percent_Identity=26.2247838616715, Blast_Score=131, Evalue=9e-32,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR022924
- InterPro:   IPR001736 [H]

Pfam domain/function: PF00614 PLDc [H]

EC number: 2.7.8.-

Molecular weight: Translated: 54121; Mature: 53990

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50035 PLD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.7 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLIPLILSTITLLNIVVVITLIFFERKNPTATWAWVLILVLFPILGLILYLFIGLTPRK
CCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
RKIFDRKTEKDLIKNKQLIEGSPNYQGKSLPHQANNIFQIDFGKEEIVYTDNNDVQIYTH
HHHHHCHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCEEEEEC
GKEKFKELFNVMETAEHHIHISYYIIRNDELGKAIVNKLTKKARQGVTVRVLYDSVGCRK
CHHHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
LPKDFFSELEEAGGLVTRFFPFILDINYRNHRKIAVIDGKTAFVGGTNIGKEYLGISQRF
CCHHHHHHHHHHCCHHHHHHHHHEEECCCCCCEEEEECCCEEEECCCCCCHHHHCHHHHC
DDWRDTHLKVTGDAAKSLQVRFLLDWIFATEEDEDIMMNREFFPEISDQGNSGIQIVASG
CCCCCCEEEEECCCCHHHHHHEEEEHHHCCCCCCCEEECHHHCCCCCCCCCCCEEEEECC
PDSPHEEIKAFLLKMIYSAKESIYLQTPYFIPDESILEALQIAAYSGVDVRIAIPDKPDH
CCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
PFVFWANRAYLGLMLETGARGFIYKHGFLHSKLIIVDGKIASVGTANMDVRSFKLNFEVN
CEEEEECCEEEEEEEECCCCCEEEECCCCEEEEEEEECCEEECCCCCCEEEEEEEEEEEE
AFIYDPQMAQRLNEIFLRDLDNSCELTEDQYNQRGISSKFKESISRLLSPIL
EEEECHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
TLIPLILSTITLLNIVVVITLIFFERKNPTATWAWVLILVLFPILGLILYLFIGLTPRK
CHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCH
RKIFDRKTEKDLIKNKQLIEGSPNYQGKSLPHQANNIFQIDFGKEEIVYTDNNDVQIYTH
HHHHHCHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCEEEEECCCCEEEEECCCCEEEEEC
GKEKFKELFNVMETAEHHIHISYYIIRNDELGKAIVNKLTKKARQGVTVRVLYDSVGCRK
CHHHHHHHHHHHHCCCCEEEEEEEEEECCCHHHHHHHHHHHHHHCCCEEEEEECCCCCCC
LPKDFFSELEEAGGLVTRFFPFILDINYRNHRKIAVIDGKTAFVGGTNIGKEYLGISQRF
CCHHHHHHHHHHCCHHHHHHHHHEEECCCCCCEEEEECCCEEEECCCCCCHHHHCHHHHC
DDWRDTHLKVTGDAAKSLQVRFLLDWIFATEEDEDIMMNREFFPEISDQGNSGIQIVASG
CCCCCCEEEEECCCCHHHHHHEEEEHHHCCCCCCCEEECHHHCCCCCCCCCCCEEEEECC
PDSPHEEIKAFLLKMIYSAKESIYLQTPYFIPDESILEALQIAAYSGVDVRIAIPDKPDH
CCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHHCCCCEEEEECCCCCCC
PFVFWANRAYLGLMLETGARGFIYKHGFLHSKLIIVDGKIASVGTANMDVRSFKLNFEVN
CEEEEECCEEEEEEEECCCCCEEEECCCCEEEEEEEECCEEECCCCCCEEEEEEEEEEEE
AFIYDPQMAQRLNEIFLRDLDNSCELTEDQYNQRGISSKFKESISRLLSPIL
EEEECHHHHHHHHHHHHHCCCCCCCCCHHHHHHCCCHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: Transferring phosphorus-containing groups [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12235376 [H]