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The map label for this gene is pycB [H]

Identifier: 188585269

GI number: 188585269

Start: 654795

End: 656696

Strand: Direct

Name: pycB [H]

Synonym: Nther_0631

Alternate gene names: 188585269

Gene position: 654795-656696 (Clockwise)

Preceding gene: 188585268

Following gene: 188585271

Centisome position: 20.68

GC content: 38.38

Gene sequence:

>1902_bases
TTGTCAGTAAAAATCACAGACACCTCGTTAAGAGATGCTCATCAGTCTCTGTTTGCTACCAGGATGAGGACAGGAGATAT
GGTGCCCATCCTTGAAACAATTGATAATGTTGGTTTCCATTCCCTGGAGATGTGGGGAGGGGCAACCTTTGATTCTTGTA
TGAGATTTTTAGATGAAGATCCTTGGGAACGGCTAAGAGTAATTCGAAAGTACGTTAAAAATACTAAGTTACAAATGTTA
TTAAGAGGTCAAAATATTGTAGGATATAAAAACTATCCCGATGATGTTTTGGAAGAATTTATTAAGAAAACAGTAGCTAA
TGGAATGGATATCATAAGAATTTTTGATGCCGTCAATGACTTAAGGAATATGGAAAAAGCCATTGAATTTACTAAACGCG
AAGGGGCTCATGCTCAAGGAACAATTTGTTACACCATTAGTCCTGTTCATAATAATAATGTATTTGTTCAGTTCGCCAAG
GATTTGAAAGAAACAGGCTGTGACTCTATTTGTGTAAAAGATATGGCAGGTCTTTTATCTCCATATAAAGGATATGATTT
GGTAAAAAGATTAAAAGAAGAAGTGGGTTTACCTGTTCAGATACACAGTCACAGTACTAGTGGAATGGCTACAACCACTT
ATATAAAGTCAATTGAAGCTGGGGCGGATATAGTAGACACTACTATATCACCTTTTGCACTGGGTACCTCACAACCTCCC
GTAGAACCAGTTGAAGCAATGTTAAGTGAAACTGAAAAAGAATTAGAATTAGATGGGGATGCTTTGACGGAGATGAGCGA
GTATTTCAAAGGAATGAAAAAGAAATACGAAAAATATTCAAAACTTGCCTTCACTGTTGATACTAATGTTTTAAATTATC
AGGTACCGGGTGGAATGATATCCAACTTAACATCTCAACTATCAGATGCCAATGCCATCGAAAAATATGATGAAGCTTTA
CAAGAAATTCCTAAAGTCAGAGAGGAATTGGGTTATCCTCCTCTGGTTACACCAACAAGCCAAATAGTGGGAAGCCAAGC
TGTACTTAATGTGTTAACTGGGGAAAGGTATAAAATGATTTCTGAGGAAGTGAAAAATTATGTAGCTGGGTATTATGGAA
AGCCTCCCCATGAAATAGATCCTACACTTAAAGAACGGATACTTCAAGAAAAAGAAGAATTCACGGGAAGGCCTGCAGAT
CATTTAGAACCACAACTTGACAAAGCTAGGAATGAAATAGAACAATATCTAGAACAAGAGGAAGATGTACTGTCTTATTG
TCTGTTCCCCAACGTGGCAATGGAATTTTTCAAAAAACGCCAAGAAGGTAAAATAAATGCTTCAGAGGATAACGTGGAAC
CAGAACCAGAAAAAGAAAAAGCTGAAGCTAGCGGTAATGGCAAGTCAGGGACAGATTCTGATACCAAGGCCCAGGGACAA
TCAGCTGCTATGCAAGGCCCAGATTCTTTAAAACAATTGCGAGTTACTGTGGACGGTCAAACCTTTGATGTGGAAGTAGA
ACCTGTAGCAGGTGGTAGTCCTTCCATTAAGTCTATACAACCTTCTCAAACTCAGACAACTAAATCAAGCCAAGCCCAAA
AACCCCAAAAACAAAAACCTCAGACAAATAACGCATCAGAGTCGGCTTCACAGGTACAGCAAGGTCAAGCTTCAGCGACT
TCAGGCACTGGTACTACTATAACTGCTCCAATAGCTGGTAATGTATTAAAAATCAATGTATCTCCTGGAGATAATGTCAA
TAAAGGAGATGTGGTCATGATTTTAGAAGCTATGAAAATGGAAAATGAGATTACTGCCGATTCAGATGGGACAGTTAAGG
ATGTTAATGTATCCGAAGGAGCCACAGTGAATTCTGGAGATCCTTTAGTTGTATTAGAATAG

Upstream 100 bases:

>100_bases
GAAGCATATAATCGAAAACAGAGAAGACTAGTTGTACTTCTATTACCATGTGCTAGCCATAGTTCATGACTGATTTATCA
AGAAAAGGGAGGTTTAAAAA

Downstream 100 bases:

>100_bases
TGTTTAAATATGTTAATTTAAAAAAAGGACCTGGTTTTTGCCGGGTCCTTCTTTAAGGGTTGTGAAACCCTACTGAACAG
TCTAAGTTTGATCTCTCTGT

Product: Conserved carboxylase region

Products: NA

Alternate protein names: Pyruvic carboxylase B [H]

Number of amino acids: Translated: 633; Mature: 632

Protein sequence:

>633_residues
MSVKITDTSLRDAHQSLFATRMRTGDMVPILETIDNVGFHSLEMWGGATFDSCMRFLDEDPWERLRVIRKYVKNTKLQML
LRGQNIVGYKNYPDDVLEEFIKKTVANGMDIIRIFDAVNDLRNMEKAIEFTKREGAHAQGTICYTISPVHNNNVFVQFAK
DLKETGCDSICVKDMAGLLSPYKGYDLVKRLKEEVGLPVQIHSHSTSGMATTTYIKSIEAGADIVDTTISPFALGTSQPP
VEPVEAMLSETEKELELDGDALTEMSEYFKGMKKKYEKYSKLAFTVDTNVLNYQVPGGMISNLTSQLSDANAIEKYDEAL
QEIPKVREELGYPPLVTPTSQIVGSQAVLNVLTGERYKMISEEVKNYVAGYYGKPPHEIDPTLKERILQEKEEFTGRPAD
HLEPQLDKARNEIEQYLEQEEDVLSYCLFPNVAMEFFKKRQEGKINASEDNVEPEPEKEKAEASGNGKSGTDSDTKAQGQ
SAAMQGPDSLKQLRVTVDGQTFDVEVEPVAGGSPSIKSIQPSQTQTTKSSQAQKPQKQKPQTNNASESASQVQQGQASAT
SGTGTTITAPIAGNVLKINVSPGDNVNKGDVVMILEAMKMENEITADSDGTVKDVNVSEGATVNSGDPLVVLE

Sequences:

>Translated_633_residues
MSVKITDTSLRDAHQSLFATRMRTGDMVPILETIDNVGFHSLEMWGGATFDSCMRFLDEDPWERLRVIRKYVKNTKLQML
LRGQNIVGYKNYPDDVLEEFIKKTVANGMDIIRIFDAVNDLRNMEKAIEFTKREGAHAQGTICYTISPVHNNNVFVQFAK
DLKETGCDSICVKDMAGLLSPYKGYDLVKRLKEEVGLPVQIHSHSTSGMATTTYIKSIEAGADIVDTTISPFALGTSQPP
VEPVEAMLSETEKELELDGDALTEMSEYFKGMKKKYEKYSKLAFTVDTNVLNYQVPGGMISNLTSQLSDANAIEKYDEAL
QEIPKVREELGYPPLVTPTSQIVGSQAVLNVLTGERYKMISEEVKNYVAGYYGKPPHEIDPTLKERILQEKEEFTGRPAD
HLEPQLDKARNEIEQYLEQEEDVLSYCLFPNVAMEFFKKRQEGKINASEDNVEPEPEKEKAEASGNGKSGTDSDTKAQGQ
SAAMQGPDSLKQLRVTVDGQTFDVEVEPVAGGSPSIKSIQPSQTQTTKSSQAQKPQKQKPQTNNASESASQVQQGQASAT
SGTGTTITAPIAGNVLKINVSPGDNVNKGDVVMILEAMKMENEITADSDGTVKDVNVSEGATVNSGDPLVVLE
>Mature_632_residues
SVKITDTSLRDAHQSLFATRMRTGDMVPILETIDNVGFHSLEMWGGATFDSCMRFLDEDPWERLRVIRKYVKNTKLQMLL
RGQNIVGYKNYPDDVLEEFIKKTVANGMDIIRIFDAVNDLRNMEKAIEFTKREGAHAQGTICYTISPVHNNNVFVQFAKD
LKETGCDSICVKDMAGLLSPYKGYDLVKRLKEEVGLPVQIHSHSTSGMATTTYIKSIEAGADIVDTTISPFALGTSQPPV
EPVEAMLSETEKELELDGDALTEMSEYFKGMKKKYEKYSKLAFTVDTNVLNYQVPGGMISNLTSQLSDANAIEKYDEALQ
EIPKVREELGYPPLVTPTSQIVGSQAVLNVLTGERYKMISEEVKNYVAGYYGKPPHEIDPTLKERILQEKEEFTGRPADH
LEPQLDKARNEIEQYLEQEEDVLSYCLFPNVAMEFFKKRQEGKINASEDNVEPEPEKEKAEASGNGKSGTDSDTKAQGQS
AAMQGPDSLKQLRVTVDGQTFDVEVEPVAGGSPSIKSIQPSQTQTTKSSQAQKPQKQKPQTNNASESASQVQQGQASATS
GTGTTITAPIAGNVLKINVSPGDNVNKGDVVMILEAMKMENEITADSDGTVKDVNVSEGATVNSGDPLVVLE

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG5016

COG function: function code C; Pyruvate/oxaloacetate carboxyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 carboxyltransferase domain [H]

Homologues:

Organism=Homo sapiens, GI106049528, Length=667, Percent_Identity=30.5847076461769, Blast_Score=265, Evalue=1e-70,
Organism=Homo sapiens, GI106049295, Length=667, Percent_Identity=30.5847076461769, Blast_Score=265, Evalue=1e-70,
Organism=Homo sapiens, GI106049292, Length=667, Percent_Identity=30.5847076461769, Blast_Score=265, Evalue=1e-70,
Organism=Caenorhabditis elegans, GI17562816, Length=463, Percent_Identity=35.6371490280778, Blast_Score=268, Evalue=7e-72,
Organism=Caenorhabditis elegans, GI17567343, Length=69, Percent_Identity=46.3768115942029, Blast_Score=66, Evalue=5e-11,
Organism=Saccharomyces cerevisiae, GI6319695, Length=662, Percent_Identity=30.3625377643505, Blast_Score=287, Evalue=3e-78,
Organism=Saccharomyces cerevisiae, GI6321376, Length=621, Percent_Identity=29.4685990338164, Blast_Score=258, Evalue=2e-69,
Organism=Drosophila melanogaster, GI24652212, Length=653, Percent_Identity=29.7090352220521, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24652210, Length=653, Percent_Identity=29.7090352220521, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24652214, Length=653, Percent_Identity=29.7090352220521, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI19921944, Length=653, Percent_Identity=29.7090352220521, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI24652216, Length=653, Percent_Identity=29.7090352220521, Blast_Score=273, Evalue=2e-73,
Organism=Drosophila melanogaster, GI281363050, Length=653, Percent_Identity=29.7090352220521, Blast_Score=273, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24652224, Length=653, Percent_Identity=29.7090352220521, Blast_Score=273, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24652222, Length=653, Percent_Identity=29.7090352220521, Blast_Score=273, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24652220, Length=653, Percent_Identity=29.7090352220521, Blast_Score=273, Evalue=3e-73,
Organism=Drosophila melanogaster, GI24652218, Length=653, Percent_Identity=29.7090352220521, Blast_Score=273, Evalue=3e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001882
- InterPro:   IPR000089
- InterPro:   IPR003379
- InterPro:   IPR005776
- InterPro:   IPR000891
- InterPro:   IPR011053 [H]

Pfam domain/function: PF00364 Biotin_lipoyl; PF00682 HMGL-like; PF02436 PYC_OADA [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 69733; Mature: 69602

Theoretical pI: Translated: 4.58; Mature: 4.58

Prosite motif: PS50991 PYR_CT ; PS50968 BIOTINYL_LIPOYL ; PS00188 BIOTIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVKITDTSLRDAHQSLFATRMRTGDMVPILETIDNVGFHSLEMWGGATFDSCMRFLDED
CEEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEHHCCCCCHHHHHHHHCCC
PWERLRVIRKYVKNTKLQMLLRGQNIVGYKNYPDDVLEEFIKKTVANGMDIIRIFDAVND
HHHHHHHHHHHHHCCHHEEEEECCCEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
LRNMEKAIEFTKREGAHAQGTICYTISPVHNNNVFVQFAKDLKETGCDSICVKDMAGLLS
HHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCEEEHHHHHHHHCCCCHHHHHHHHHHHC
PYKGYDLVKRLKEEVGLPVQIHSHSTSGMATTTYIKSIEAGADIVDTTISPFALGTSQPP
CCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCC
VEPVEAMLSETEKELELDGDALTEMSEYFKGMKKKYEKYSKLAFTVDTNVLNYQVPGGMI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEECCCHHH
SNLTSQLSDANAIEKYDEALQEIPKVREELGYPPLVTPTSQIVGSQAVLNVLTGERYKMI
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHH
SEEVKNYVAGYYGKPPHEIDPTLKERILQEKEEFTGRPADHLEPQLDKARNEIEQYLEQE
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHH
EDVLSYCLFPNVAMEFFKKRQEGKINASEDNVEPEPEKEKAEASGNGKSGTDSDTKAQGQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHCCC
SAAMQGPDSLKQLRVTVDGQTFDVEVEPVAGGSPSIKSIQPSQTQTTKSSQAQKPQKQKP
HHHCCCCHHHHHEEEEECCEEEEEEEEECCCCCCCCCCCCCCCHHHCCHHHHCCCHHCCC
QTNNASESASQVQQGQASATSGTGTTITAPIAGNVLKINVSPGDNVNKGDVVMILEAMKM
CCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCEEEHHHHHHH
ENEITADSDGTVKDVNVSEGATVNSGDPLVVLE
CCCCCCCCCCCEEECCCCCCCEECCCCCEEEEC
>Mature Secondary Structure 
SVKITDTSLRDAHQSLFATRMRTGDMVPILETIDNVGFHSLEMWGGATFDSCMRFLDED
EEEEECCHHHHHHHHHHHHHHCCCCCHHHHHHHHCCCCCEEHHCCCCCHHHHHHHHCCC
PWERLRVIRKYVKNTKLQMLLRGQNIVGYKNYPDDVLEEFIKKTVANGMDIIRIFDAVND
HHHHHHHHHHHHHCCHHEEEEECCCEECCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHH
LRNMEKAIEFTKREGAHAQGTICYTISPVHNNNVFVQFAKDLKETGCDSICVKDMAGLLS
HHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCEEEHHHHHHHHCCCCHHHHHHHHHHHC
PYKGYDLVKRLKEEVGLPVQIHSHSTSGMATTTYIKSIEAGADIVDTTISPFALGTSQPP
CCCCHHHHHHHHHHCCCCEEEECCCCCCCHHHHHHHHHHCCCCHHHCCCCCCCCCCCCCC
VEPVEAMLSETEKELELDGDALTEMSEYFKGMKKKYEKYSKLAFTVDTNVLNYQVPGGMI
HHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCEEEEECCCHHH
SNLTSQLSDANAIEKYDEALQEIPKVREELGYPPLVTPTSQIVGSQAVLNVLTGERYKMI
HHHHHHHHCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHCCHHHHHH
SEEVKNYVAGYYGKPPHEIDPTLKERILQEKEEFTGRPADHLEPQLDKARNEIEQYLEQE
HHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHCCCCHHHCCHHHHHHHHHHHHHHHHH
EDVLSYCLFPNVAMEFFKKRQEGKINASEDNVEPEPEKEKAEASGNGKSGTDSDTKAQGQ
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCCHHHCCC
SAAMQGPDSLKQLRVTVDGQTFDVEVEPVAGGSPSIKSIQPSQTQTTKSSQAQKPQKQKP
HHHCCCCHHHHHEEEEECCEEEEEEEEECCCCCCCCCCCCCCCHHHCCHHHHCCCHHCCC
QTNNASESASQVQQGQASATSGTGTTITAPIAGNVLKINVSPGDNVNKGDVVMILEAMKM
CCCCCHHHHHHHHHCCCCCCCCCCCEEEEECCCCEEEEECCCCCCCCCCCEEEHHHHHHH
ENEITADSDGTVKDVNVSEGATVNSGDPLVVLE
CCCCCCCCCCCEEECCCCCCCEECCCCCEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8688087; 11195096 [H]