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The map label for this gene is dat [H]

Identifier: 188585268

GI number: 188585268

Start: 653871

End: 654725

Strand: Direct

Name: dat [H]

Synonym: Nther_0630

Alternate gene names: 188585268

Gene position: 653871-654725 (Clockwise)

Preceding gene: 188585267

Following gene: 188585269

Centisome position: 20.66

GC content: 40.82

Gene sequence:

>855_bases
ATGTCACAAGCACTTGCTTATGTAAATGGGGATTTTGTGGAGCTAGAAAAGGCAAAAATAGATGCCCTGGATAGGGGGTT
TATTTTTGGTGATGGGCTGTATGAAGTAGTAGCTATTTTTGGTGGAAAAATGTATCAATTGGATGAGCATCTGCAGCGGT
ATTGGGATGGAGCTCAAGAAATGATGTTTGAGAACACTCCCCGACCTGAAGATTTAAAGAAAGCAGCTCGTGAACTTCTT
CAAAAAACTGGTATTGGTGAAGGAATCATTTATTTAGAGGTTACTAGAGGCACAGCACCCAGAAGTCATCGTTTCCCCCA
AGAACCGGAACCAAATGTATTCATGTTTGCTAAAGAAGCACAGTTCCCCGACGGTGAAAAACGAACTAAAGGTGTCAAGA
CAATTTTAGTCCCTGATGAACGATGGAATAGATGCCATGTAAAAAGTATCAATCTATTGCCAAATTGTTTTTATAAAGAA
AAGGCCAAGCGAGCAGGAGCATATGAGGCTATACAGGTACACCGTTTGGGAATAACGGAAGGAACTAGCACTAATATCTA
TGGGGTAAAAGACGGGGTGATATATACAGCTCCAGCGGGTCCCAGAATCTTAAAAGGAATTACCAGGACTACAGTATTGG
AGTTGGCAGAACAACAAGGAATTGAAGTTGTGGAGAAGTTTATGACAACAGGAGAACTGTTAAGCTGTGACGAAGTATTC
CTTACAAGTACTACTAATAAAGTCTTGCCTATAAATCAGGTAGATCAAGTAACCTTCCCTGTCGACCAGTATCGTGTCAC
TTTCACCTTACAGGAAGAACTAGAGAAGCATATAATCGAAAACAGAGAAGACTAG

Upstream 100 bases:

>100_bases
GTGCTGGCCGATGAAACTGTTTCTAGAGCCCTGAAAACTGGTGACAAATTGGAAATAGGTAAAAATTAAAAAAATTATAA
AATTTTCAGGAGGTGTCTTC

Downstream 100 bases:

>100_bases
TTGTACTTCTATTACCATGTGCTAGCCATAGTTCATGACTGATTTATCAAGAAAAGGGAGGTTTAAAAATTGTCAGTAAA
AATCACAGACACCTCGTTAA

Product: aminotransferase class IV

Products: NA

Alternate protein names: D-amino acid aminotransferase; D-amino acid transaminase; DAAT; D-aspartate aminotransferase [H]

Number of amino acids: Translated: 284; Mature: 283

Protein sequence:

>284_residues
MSQALAYVNGDFVELEKAKIDALDRGFIFGDGLYEVVAIFGGKMYQLDEHLQRYWDGAQEMMFENTPRPEDLKKAARELL
QKTGIGEGIIYLEVTRGTAPRSHRFPQEPEPNVFMFAKEAQFPDGEKRTKGVKTILVPDERWNRCHVKSINLLPNCFYKE
KAKRAGAYEAIQVHRLGITEGTSTNIYGVKDGVIYTAPAGPRILKGITRTTVLELAEQQGIEVVEKFMTTGELLSCDEVF
LTSTTNKVLPINQVDQVTFPVDQYRVTFTLQEELEKHIIENRED

Sequences:

>Translated_284_residues
MSQALAYVNGDFVELEKAKIDALDRGFIFGDGLYEVVAIFGGKMYQLDEHLQRYWDGAQEMMFENTPRPEDLKKAARELL
QKTGIGEGIIYLEVTRGTAPRSHRFPQEPEPNVFMFAKEAQFPDGEKRTKGVKTILVPDERWNRCHVKSINLLPNCFYKE
KAKRAGAYEAIQVHRLGITEGTSTNIYGVKDGVIYTAPAGPRILKGITRTTVLELAEQQGIEVVEKFMTTGELLSCDEVF
LTSTTNKVLPINQVDQVTFPVDQYRVTFTLQEELEKHIIENRED
>Mature_283_residues
SQALAYVNGDFVELEKAKIDALDRGFIFGDGLYEVVAIFGGKMYQLDEHLQRYWDGAQEMMFENTPRPEDLKKAARELLQ
KTGIGEGIIYLEVTRGTAPRSHRFPQEPEPNVFMFAKEAQFPDGEKRTKGVKTILVPDERWNRCHVKSINLLPNCFYKEK
AKRAGAYEAIQVHRLGITEGTSTNIYGVKDGVIYTAPAGPRILKGITRTTVLELAEQQGIEVVEKFMTTGELLSCDEVFL
TSTTNKVLPINQVDQVTFPVDQYRVTFTLQEELEKHIIENRED

Specific function: Acts on the D-isomers of alanine, leucine, aspartate, glutamate, aminobutyrate, norvaline and asparagine. The enzyme transfers an amino group from a substrate D-amino acid to the pyridoxal phosphate cofactor to form pyridoxamine and an alpha- keto acid in

COG id: COG0115

COG function: function code EH; Branched-chain amino acid aminotransferase/4-amino-4-deoxychorismate lyase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-IV pyridoxal-phosphate-dependent aminotransferase family [H]

Homologues:

Organism=Escherichia coli, GI48994963, Length=269, Percent_Identity=27.5092936802974, Blast_Score=104, Evalue=5e-24,
Organism=Escherichia coli, GI1787338, Length=244, Percent_Identity=25, Blast_Score=72, Evalue=5e-14,

Paralogues:

None

Copy number: 2342 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 11,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001544
- InterPro:   IPR018300
- InterPro:   IPR005784 [H]

Pfam domain/function: PF01063 Aminotran_4 [H]

EC number: =2.6.1.21 [H]

Molecular weight: Translated: 32254; Mature: 32123

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: PS00770 AA_TRANSFER_CLASS_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSQALAYVNGDFVELEKAKIDALDRGFIFGDGLYEVVAIFGGKMYQLDEHLQRYWDGAQE
CCCEEEEECCCEEEEHHHHHHHHHCCEEECCHHHHHHHHHCCCHHHHHHHHHHHHCHHHH
MMFENTPRPEDLKKAARELLQKTGIGEGIIYLEVTRGTAPRSHRFPQEPEPNVFMFAKEA
HHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECC
QFPDGEKRTKGVKTILVPDERWNRCHVKSINLLPNCFYKEKAKRAGAYEAIQVHRLGITE
CCCCCCHHCCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHCCCHHEEEEEEEECCC
GTSTNIYGVKDGVIYTAPAGPRILKGITRTTVLELAEQQGIEVVEKFMTTGELLSCDEVF
CCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEE
LTSTTNKVLPINQVDQVTFPVDQYRVTFTLQEELEKHIIENRED
EECCCCCEECCCCCCCEEECCCCEEEEEEHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SQALAYVNGDFVELEKAKIDALDRGFIFGDGLYEVVAIFGGKMYQLDEHLQRYWDGAQE
CCEEEEECCCEEEEHHHHHHHHHCCEEECCHHHHHHHHHCCCHHHHHHHHHHHHCHHHH
MMFENTPRPEDLKKAARELLQKTGIGEGIIYLEVTRGTAPRSHRFPQEPEPNVFMFAKEA
HHHCCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCCCCCCCCCCCCCEEEEEECC
QFPDGEKRTKGVKTILVPDERWNRCHVKSINLLPNCFYKEKAKRAGAYEAIQVHRLGITE
CCCCCCHHCCCCEEEEECCCCCCEEEEEEECCCCCHHHHHHHHHCCCHHEEEEEEEECCC
GTSTNIYGVKDGVIYTAPAGPRILKGITRTTVLELAEQQGIEVVEKFMTTGELLSCDEVF
CCCCCEEEECCCEEEECCCCHHHHHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCCEE
LTSTTNKVLPINQVDQVTFPVDQYRVTFTLQEELEKHIIENRED
EECCCCCEECCCCCCCEEECCCCEEEEEEHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9003455 [H]