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The map label for this gene is fdnH [H]

Identifier: 188585177

GI number: 188585177

Start: 560632

End: 561486

Strand: Direct

Name: fdnH [H]

Synonym: Nther_0538

Alternate gene names: 188585177

Gene position: 560632-561486 (Clockwise)

Preceding gene: 188585176

Following gene: 188585178

Centisome position: 17.71

GC content: 38.36

Gene sequence:

>855_bases
ATGAAAGAGAACAAGGGGTTTAAGCTCAAAAGACGTCAATTTATTCAGTCTATGGCTGGTACAGGCTTATTAGCAGGTCT
TAGCCTTTCAGCTCCGCGCATAGCTGAAGCTGGTGAAAATAACGGATCCGAGTATGCAATGATGGTTGACAAAACGGAAT
GCGTTGGTTGTAACGCTTGTACCAGGGTGTGTTCAGAAGCTTATGAATTGTCTGAAGGTATTTTAAGAACTGAGATCCAC
AGATTCAAAGATGATGAAGACGAAGAATACTTTAAGAAAAATGCTTGTCTTCACTGTAATGAGGCTAGCTGTGTCATGGC
TTGTCCTGCAGATGCAATTTATAAAGATGATTTGGGATTAACCCAAATTGATAACAGTATTTGTGTTAATTGTGGTTATT
GCGTATCTGCTTGCCCTTACAACGCTATTAAGTATGATCGCAAAAAAGGTGTTAATGAGAAATGTACTTTATGTACTGAA
CTTATTAATGCTGGAAGTGAACCTTTGTGTAAGCAAGAATGTCCTGTAGATGCTATTTACTTTGGTACTAGAGAAGAAAT
GCTAGAGCTGGGACGAGAAAAAGTTTCCGATTTAAAAGACCAGGGATATGATAAAGCCATGTTATATGGTGATGATGAAT
TTGATGGTTTGGCAGTTTTAGGAGTTTTACATGATGAGCCCCAAGAGTACGAGTTGCCAAAGGATCCTCAAATACCTATG
CAATTAAGGGTATGGAATCAAATACCTTTTGCACCAGCCTTTTTAGTAGTAGGTGGGGCGGCTATGGTATTTAATTTCTT
ACATTCAAGAAAAACCAAAGCCGAATTAGAACAACCAGAAGAAGATGAAGAATAA

Upstream 100 bases:

>100_bases
TTTTATGCTTTAATTAGTGACAGATGTAACAGCAAGATGTAACAATTAAACCGCTTCTAGTATTAGCTGAATTTTATTAG
ATGGGAGGGAGAAAGGGAAT

Downstream 100 bases:

>100_bases
ATCTTAATTTTACTTAACTAGGGGGTGGAGAGTATGGAAGCTAAAACTTTTCCGGGAGGAGCATATATACCTCATTATAA
ATTTACTGACGACAAGCCGA

Product: 4Fe-4S ferredoxin iron-sulfur binding domain protein

Products: CO2; NADH2 [C]

Alternate protein names: Anaerobic formate dehydrogenase iron-sulfur subunit; Formate dehydrogenase-N subunit beta; FDH-N subunit beta [H]

Number of amino acids: Translated: 284; Mature: 284

Protein sequence:

>284_residues
MKENKGFKLKRRQFIQSMAGTGLLAGLSLSAPRIAEAGENNGSEYAMMVDKTECVGCNACTRVCSEAYELSEGILRTEIH
RFKDDEDEEYFKKNACLHCNEASCVMACPADAIYKDDLGLTQIDNSICVNCGYCVSACPYNAIKYDRKKGVNEKCTLCTE
LINAGSEPLCKQECPVDAIYFGTREEMLELGREKVSDLKDQGYDKAMLYGDDEFDGLAVLGVLHDEPQEYELPKDPQIPM
QLRVWNQIPFAPAFLVVGGAAMVFNFLHSRKTKAELEQPEEDEE

Sequences:

>Translated_284_residues
MKENKGFKLKRRQFIQSMAGTGLLAGLSLSAPRIAEAGENNGSEYAMMVDKTECVGCNACTRVCSEAYELSEGILRTEIH
RFKDDEDEEYFKKNACLHCNEASCVMACPADAIYKDDLGLTQIDNSICVNCGYCVSACPYNAIKYDRKKGVNEKCTLCTE
LINAGSEPLCKQECPVDAIYFGTREEMLELGREKVSDLKDQGYDKAMLYGDDEFDGLAVLGVLHDEPQEYELPKDPQIPM
QLRVWNQIPFAPAFLVVGGAAMVFNFLHSRKTKAELEQPEEDEE
>Mature_284_residues
MKENKGFKLKRRQFIQSMAGTGLLAGLSLSAPRIAEAGENNGSEYAMMVDKTECVGCNACTRVCSEAYELSEGILRTEIH
RFKDDEDEEYFKKNACLHCNEASCVMACPADAIYKDDLGLTQIDNSICVNCGYCVSACPYNAIKYDRKKGVNEKCTLCTE
LINAGSEPLCKQECPVDAIYFGTREEMLELGREKVSDLKDQGYDKAMLYGDDEFDGLAVLGVLHDEPQEYELPKDPQIPM
QLRVWNQIPFAPAFLVVGGAAMVFNFLHSRKTKAELEQPEEDEE

Specific function: Formate dehydrogenase allows E.coli to use formate as major electron donor during anaerobic respiration, when nitrate is used as electron acceptor. The beta chain is an electron transfer unit containing 4 cysteine clusters involved in the formation of iro

COG id: COG0437

COG function: function code C; Fe-S-cluster-containing hydrogenase components 1

Gene ontology:

Cell location: Cell membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 4 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787749, Length=267, Percent_Identity=32.9588014981273, Blast_Score=161, Evalue=4e-41,
Organism=Escherichia coli, GI1790326, Length=266, Percent_Identity=32.7067669172932, Blast_Score=154, Evalue=5e-39,
Organism=Escherichia coli, GI2367345, Length=195, Percent_Identity=34.8717948717949, Blast_Score=114, Evalue=7e-27,
Organism=Escherichia coli, GI226510944, Length=210, Percent_Identity=30.4761904761905, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1789370, Length=260, Percent_Identity=29.2307692307692, Blast_Score=105, Evalue=2e-24,
Organism=Escherichia coli, GI1787872, Length=158, Percent_Identity=29.1139240506329, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787122, Length=167, Percent_Identity=29.3413173652695, Blast_Score=100, Evalue=1e-22,
Organism=Escherichia coli, GI1787740, Length=89, Percent_Identity=38.2022471910112, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI87082180, Length=141, Percent_Identity=30.4964539007092, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1788811, Length=148, Percent_Identity=29.7297297297297, Blast_Score=69, Evalue=2e-13,
Organism=Escherichia coli, GI1787478, Length=89, Percent_Identity=37.0786516853933, Blast_Score=69, Evalue=3e-13,
Organism=Escherichia coli, GI1789079, Length=106, Percent_Identity=32.0754716981132, Blast_Score=67, Evalue=1e-12,
Organism=Escherichia coli, GI87082179, Length=155, Percent_Identity=27.741935483871, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI2367245, Length=139, Percent_Identity=28.7769784172662, Blast_Score=63, Evalue=2e-11,
Organism=Escherichia coli, GI87082114, Length=106, Percent_Identity=29.2452830188679, Blast_Score=62, Evalue=5e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR006470
- InterPro:   IPR014603
- InterPro:   IPR015246 [H]

Pfam domain/function: PF09163 Form-deh_trans [H]

EC number: NA

Molecular weight: Translated: 31727; Mature: 31727

Theoretical pI: Translated: 4.40; Mature: 4.40

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

5.6 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
8.8 %Cys+Met (Translated Protein)
5.6 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
8.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKENKGFKLKRRQFIQSMAGTGLLAGLSLSAPRIAEAGENNGSEYAMMVDKTECVGCNAC
CCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHCCCCCCCEEEEEEECCHHCCCHHH
TRVCSEAYELSEGILRTEIHRFKDDEDEEYFKKNACLHCNEASCVMACPADAIYKDDLGL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCCCEEEECCCCHHHHCCCCC
TQIDNSICVNCGYCVSACPYNAIKYDRKKGVNEKCTLCTELINAGSEPLCKQECPVDAIY
EEECCCEEEECCCHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCCCCEEE
FGTREEMLELGREKVSDLKDQGYDKAMLYGDDEFDGLAVLGVLHDEPQEYELPKDPQIPM
ECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCE
QLRVWNQIPFAPAFLVVGGAAMVFNFLHSRKTKAELEQPEEDEE
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure
MKENKGFKLKRRQFIQSMAGTGLLAGLSLSAPRIAEAGENNGSEYAMMVDKTECVGCNAC
CCCCCCCCHHHHHHHHHHHCCCEEECCCCCCCHHHHCCCCCCCEEEEEEECCHHCCCHHH
TRVCSEAYELSEGILRTEIHRFKDDEDEEYFKKNACLHCNEASCVMACPADAIYKDDLGL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHCCCEEECCCCEEEECCCCHHHHCCCCC
TQIDNSICVNCGYCVSACPYNAIKYDRKKGVNEKCTLCTELINAGSEPLCKQECPVDAIY
EEECCCEEEECCCHHHHCCCHHHHHHHHCCCCHHHHHHHHHHHCCCCCCHHHCCCCCEEE
FGTREEMLELGREKVSDLKDQGYDKAMLYGDDEFDGLAVLGVLHDEPQEYELPKDPQIPM
ECCHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCEEEEEEECCCCCCCCCCCCCCCCE
QLRVWNQIPFAPAFLVVGGAAMVFNFLHSRKTKAELEQPEEDEE
EEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: Fe [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: formate; NAD [C]

Specific reaction: formate + NAD = CO2 + NADH2 [C]

General reaction: Oxidoreductases; Acting on the aldehyde or oxo group of donors [C]

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1834669; 9097039; 9278503; 11884747 [H]