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The map label for this gene is rnfC [H]

Identifier: 188585178

GI number: 188585178

Start: 561520

End: 562836

Strand: Direct

Name: rnfC [H]

Synonym: Nther_0539

Alternate gene names: 188585178

Gene position: 561520-562836 (Clockwise)

Preceding gene: 188585177

Following gene: 188585179

Centisome position: 17.74

GC content: 39.86

Gene sequence:

>1317_bases
ATGGAAGCTAAAACTTTTCCGGGAGGAGCATATATACCTCATTATAAATTTACTGACGACAAGCCGATTGAGGACATGTC
TATTCCCGAAGAGATAGTAATTCCCTTAGCCCAGCATATTGGATCCCCATGTGAACCGCTGGTAAAAGTTGGTGATAGGG
TTGAAGTTGGTCAAAAAATTGGTGATTCAGAGGAATATATTACCGCACCAGTACATGCCAGCGTTTCAGGAGAAGTTGTG
GCTATAGAGGAAAGGCCGTTTTTTGAAGGGGATGAAGTTGAAAGCATTGTCATTAAGCCTGATAGTGAACAGCAGGAGTT
TTCTTATGATGAGAAGAATATTGAAGATATGAGCTTAGATGATATGAAAGATAGAATTAGAGAAGCAGGAGTAGTAGGAA
TGGGTGGTGCAGCTTTCCCGACTTATGTAAACATCAATCAATCTAAGCCCATCGATAGTTTGTTGATCAATGGAGCTGAA
TGTGAACCATTTCTAACATGCGATCATAGACAAATGGTCGAGCGCAGTGAGGAATTAGTTATCGGAGCAGAAATTCTAAT
GAATATTATAGGTGCCAAAAAATGCTATTTTGGTATTGAAGAAAATAAACCTGATGCAATTGATGCAGTTTCCAAGCAAA
TCGAGGGTAAAGACGGTATGGAAGTTGTTCCTCTTAAGACAAAATATCCTCAAGGATATAAAGTTAATGTAATCCATGCA
TGCACCGGTCGAGATGTTCCTCGGGGAGTCAGATCCGCTGACTTAGGTTGTATAGTTAGAAATGTAGGAACCACTATTGC
TACCTATGAAGCCGTCAAATACGGTAAACCATTAATGGAACGAGTTGTAACTGTAAGTGGTTATAATATTCCTGAAGCTG
GTAACTACAATATTAAAATTGGCACACCCGTTGAGCATGTTTTAAGAGAGTGTGGAGTAGATAACTTTGAAAATCAGAAG
GTGATTCTTGGTGGGCCAATGACTGGTACCGCTGTCAAAGATATCAGATCTTCAGTAGTAAAAAATAGCACAGGTTTATT
ACTATTTCCTGAAGATATGACCTGGGACTACACCGACTACAATGAATGTGTCCGTTGTGGTAAATGTGTAGAACGATGTC
CTGTTCATCTATATCCCAATAAGATAGGGATGTATGCTGAACACAAACAATACAGTAAAGCCGAGGAATGGGATATAATG
GACTGTGGTGAGTGTGGTACATGTGTATACTTCTGTCCTGCTAACCGACCAATTTTAATGTGGATCCAGCAAGTTAAGCC
CACTGTTAAAAGGAGACAACGGCAACGAAGTAAATAG

Upstream 100 bases:

>100_bases
ATTTAATTTCTTACATTCAAGAAAAACCAAAGCCGAATTAGAACAACCAGAAGAAGATGAAGAATAAATCTTAATTTTAC
TTAACTAGGGGGTGGAGAGT

Downstream 100 bases:

>100_bases
ATTAGAAGTTAGAACCTGAGTATTGTGAAGAAATCGTGTTGTAATTTTTGGAGAAGGGGGAATCCACAGTGGAATCGAAT
AAAACCATTGTTTCACCGTC

Product: electron transport complex, RnfABCDGE type, C subunit

Products: NA

Alternate protein names: Nitrogen fixation protein rnfC [H]

Number of amino acids: Translated: 438; Mature: 438

Protein sequence:

>438_residues
MEAKTFPGGAYIPHYKFTDDKPIEDMSIPEEIVIPLAQHIGSPCEPLVKVGDRVEVGQKIGDSEEYITAPVHASVSGEVV
AIEERPFFEGDEVESIVIKPDSEQQEFSYDEKNIEDMSLDDMKDRIREAGVVGMGGAAFPTYVNINQSKPIDSLLINGAE
CEPFLTCDHRQMVERSEELVIGAEILMNIIGAKKCYFGIEENKPDAIDAVSKQIEGKDGMEVVPLKTKYPQGYKVNVIHA
CTGRDVPRGVRSADLGCIVRNVGTTIATYEAVKYGKPLMERVVTVSGYNIPEAGNYNIKIGTPVEHVLRECGVDNFENQK
VILGGPMTGTAVKDIRSSVVKNSTGLLLFPEDMTWDYTDYNECVRCGKCVERCPVHLYPNKIGMYAEHKQYSKAEEWDIM
DCGECGTCVYFCPANRPILMWIQQVKPTVKRRQRQRSK

Sequences:

>Translated_438_residues
MEAKTFPGGAYIPHYKFTDDKPIEDMSIPEEIVIPLAQHIGSPCEPLVKVGDRVEVGQKIGDSEEYITAPVHASVSGEVV
AIEERPFFEGDEVESIVIKPDSEQQEFSYDEKNIEDMSLDDMKDRIREAGVVGMGGAAFPTYVNINQSKPIDSLLINGAE
CEPFLTCDHRQMVERSEELVIGAEILMNIIGAKKCYFGIEENKPDAIDAVSKQIEGKDGMEVVPLKTKYPQGYKVNVIHA
CTGRDVPRGVRSADLGCIVRNVGTTIATYEAVKYGKPLMERVVTVSGYNIPEAGNYNIKIGTPVEHVLRECGVDNFENQK
VILGGPMTGTAVKDIRSSVVKNSTGLLLFPEDMTWDYTDYNECVRCGKCVERCPVHLYPNKIGMYAEHKQYSKAEEWDIM
DCGECGTCVYFCPANRPILMWIQQVKPTVKRRQRQRSK
>Mature_438_residues
MEAKTFPGGAYIPHYKFTDDKPIEDMSIPEEIVIPLAQHIGSPCEPLVKVGDRVEVGQKIGDSEEYITAPVHASVSGEVV
AIEERPFFEGDEVESIVIKPDSEQQEFSYDEKNIEDMSLDDMKDRIREAGVVGMGGAAFPTYVNINQSKPIDSLLINGAE
CEPFLTCDHRQMVERSEELVIGAEILMNIIGAKKCYFGIEENKPDAIDAVSKQIEGKDGMEVVPLKTKYPQGYKVNVIHA
CTGRDVPRGVRSADLGCIVRNVGTTIATYEAVKYGKPLMERVVTVSGYNIPEAGNYNIKIGTPVEHVLRECGVDNFENQK
VILGGPMTGTAVKDIRSSVVKNSTGLLLFPEDMTWDYTDYNECVRCGKCVERCPVHLYPNKIGMYAEHKQYSKAEEWDIM
DCGECGTCVYFCPANRPILMWIQQVKPTVKRRQRQRSK

Specific function: Required for nitrogen fixation. May be part of a membrane complex functioning as an intermediate in the electron transport to nitrogenase. Stabilizes rnfB [H]

COG id: COG4656

COG function: function code C; Predicted NADH:ubiquinone oxidoreductase, subunit RnfC

Gene ontology:

Cell location: Cell inner membrane; Peripheral membrane protein (Probable) [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 4Fe-4S ferredoxin-type domains [H]

Homologues:

Organism=Escherichia coli, GI1787916, Length=439, Percent_Identity=34.624145785877, Blast_Score=264, Evalue=9e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017896
- InterPro:   IPR017900
- InterPro:   IPR010208
- InterPro:   IPR012285
- InterPro:   IPR009051
- InterPro:   IPR011538
- InterPro:   IPR019554 [H]

Pfam domain/function: PF01512 Complex1_51K; PF10531 SLBB [H]

EC number: NA

Molecular weight: Translated: 48866; Mature: 48866

Theoretical pI: Translated: 4.79; Mature: 4.79

Prosite motif: PS00198 4FE4S_FERREDOXIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
6.6 %Cys+Met (Translated Protein)
3.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
6.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEAKTFPGGAYIPHYKFTDDKPIEDMSIPEEIVIPLAQHIGSPCEPLVKVGDRVEVGQKI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHC
GDSEEYITAPVHASVSGEVVAIEERPFFEGDEVESIVIKPDSEQQEFSYDEKNIEDMSLD
CCCCCEEEECEEECCCCCEEEEECCCCCCCCCCCEEEECCCCCHHHCCCCCCCCCCCCHH
DMKDRIREAGVVGMGGAAFPTYVNINQSKPIDSLLINGAECEPFLTCDHRQMVERSEELV
HHHHHHHHCCEEECCCCCCCEEEECCCCCCHHHHHCCCCCCCCEEECCHHHHHHHHHHEE
IGAEILMNIIGAKKCYFGIEENKPDAIDAVSKQIEGKDGMEVVPLKTKYPQGYKVNVIHA
EHHHHHHHHHCCHHHEECCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEEE
CTGRDVPRGVRSADLGCIVRNVGTTIATYEAVKYGKPLMERVVTVSGYNIPEAGNYNIKI
ECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHEECCCCCCCCCCCEEEEE
GTPVEHVLRECGVDNFENQKVILGGPMTGTAVKDIRSSVVKNSTGLLLFPEDMTWDYTDY
CCCHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCH
NECVRCGKCVERCPVHLYPNKIGMYAEHKQYSKAEEWDIMDCGECGTCVYFCPANRPILM
HHHHHHHHHHHHCCCEECCCCCCCCCHHHHHCCCCCCCCEECCCCCCEEEECCCCCCHHH
WIQQVKPTVKRRQRQRSK
HHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MEAKTFPGGAYIPHYKFTDDKPIEDMSIPEEIVIPLAQHIGSPCEPLVKVGDRVEVGQKI
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHC
GDSEEYITAPVHASVSGEVVAIEERPFFEGDEVESIVIKPDSEQQEFSYDEKNIEDMSLD
CCCCCEEEECEEECCCCCEEEEECCCCCCCCCCCEEEECCCCCHHHCCCCCCCCCCCCHH
DMKDRIREAGVVGMGGAAFPTYVNINQSKPIDSLLINGAECEPFLTCDHRQMVERSEELV
HHHHHHHHCCEEECCCCCCCEEEECCCCCCHHHHHCCCCCCCCEEECCHHHHHHHHHHEE
IGAEILMNIIGAKKCYFGIEENKPDAIDAVSKQIEGKDGMEVVPLKTKYPQGYKVNVIHA
EHHHHHHHHHCCHHHEECCCCCCCHHHHHHHHHCCCCCCCEEEEEECCCCCCEEEEEEEE
CTGRDVPRGVRSADLGCIVRNVGTTIATYEAVKYGKPLMERVVTVSGYNIPEAGNYNIKI
ECCCCCCCCCHHHHHHHHHHHHCCHHHHHHHHHHCHHHHHHHEECCCCCCCCCCCEEEEE
GTPVEHVLRECGVDNFENQKVILGGPMTGTAVKDIRSSVVKNSTGLLLFPEDMTWDYTDY
CCCHHHHHHHCCCCCCCCCEEEEECCCCCHHHHHHHHHHHCCCCCEEEECCCCCCCCCCH
NECVRCGKCVERCPVHLYPNKIGMYAEHKQYSKAEEWDIMDCGECGTCVYFCPANRPILM
HHHHHHHHHHHHCCCEECCCCCCCCCHHHHHCCCCCCCCEECCCCCCEEEECCCCCCHHH
WIQQVKPTVKRRQRQRSK
HHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: 4Fe-4S clusters [C]

Metal ions: Iron-Sulfur [C]

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8264535; 9492268; 9154934 [H]