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The map label for this gene is appA [H]

Identifier: 188585116

GI number: 188585116

Start: 507157

End: 508878

Strand: Direct

Name: appA [H]

Synonym: Nther_0477

Alternate gene names: 188585116

Gene position: 507157-508878 (Clockwise)

Preceding gene: 188585115

Following gene: 188585117

Centisome position: 16.02

GC content: 39.9

Gene sequence:

>1722_bases
ATGAGTAGCAGAAAGTTATTTGTATTATTTCTGGCATTGATGTTTGCTACTGCTGTAGCCATCGGTGGCTGTGCACCTCC
AGAAGAAGCTAAAGAGGAGGATCCAGAAGCAGAGGAAGAAGAAGAGGCTCCAGCAGAAGTAGATAATCCTGCTGTAGAAA
GACCTAATGAAATTGTAATTGGAGGTCCAGATCTAGAAGGAATTTTCAATCCAGTTTTATACAGTGGTGTTTATGACTCC
TGGGTTTTAGGTATGATTTTTGATTCACTACTTACAGTAGATGAAAATGGACAGTTAACAACTGATCAGAGATCAGTTGC
CAAAGATTATGAAATCTCAGAAGATGGCACTGTATACACTTTCCACTTACGTGAAGGATGGGAATTTCACGATGGAGAAG
AGATAACTGCCGAAGATGTGGCCTTTTCTTTAGAAGTTACTGCTCACCCTGATTACGACGGACCACGCTCCAGCTTTTCC
GACGATATAGTGGGTGTAGATGAATTTCGAGAAGGAGAAACTGACGAGCTAGAAGGAATTACAGTAGAAGATGATTATAC
CTTGGTAGTTGAAGCTCAAGAACCTAGTGCTGATAACATATTTGATTTTGCAGTAATGATAATGCCAAAACATTATTATG
ATTTTGAAAACTATGAAGACTTCCAGGATTTAACAGACGACCCAATGGGAAGTGGTCCCTTCGAACTTGTAGAATACAGT
CCGGATCAACATGCTATCTTAGAATCCTTTGAAGACTATTACCATGGTGCACCAAATGTGGATCGAATAATCTACGAAGA
AACTGAAACTGAACAACAAATTCCAATGGTTGAAACAGGAGAAGCTGATATTGTCCAGGTAAGTTCAACTCCAGAAAATA
TGGAAATGTTAGAAGATATAGACCATCAGGAAGCATTAACTTTCTTTGATAATGCCTATACTTATATTGGTTTGAACCAC
GAAGATGAACACTTGCAGCATCAAGAGGTTAGGCAGGCATTAGCTTATGCCCTTGATATCGAAGCTTTCCTTGATGGAAT
GTTTGGAGATGAATTGGCTCAACCGATATACACTCCGTTTTCACCCGTATCATGGGCATATCCCGATGAAGACGATATGA
ACGATTATGCTTATGACCCTGATAAGGCCAATGAACTACTTGATGAAGCGGGTTATGAGTGGGATGACAATGAAGAGTAT
CGAGTAAATGAAGACGGAGAGAGACTATCCTTTACATGGGAAACCATTGCTGACAACGAATGGTCCGAACATTTAACTAC
TCTAGCCTTAGAACAATGGCCACAAATAGGAGTAGAACTAGAAATCGAAAACTACGATTTCAATACCTTGACCGACAGAA
TTGATCAGGACCGCGGAGATGTAGATATGTGGAATATGGGTTGGTCACTATCAGCCGAACCCGATCCATCTAATATCTTT
AGTGTAGAATATGCCGATGCTGGCTTAAACTACGGAATGTATCACAACGAAGAAGCAGAAGAATTGATGCAAGAAGGTCT
TGAAACCTTTGATCAAGATGAAAGAGCAGAAGTTTACAATGAACTAGGTGTACTATTTAATGAAGATCTACCCTATATCT
TTGTCTACAGTAACAAAGAGATTTGGTCCACAAATGACCGAGTTGAAAACTATGAACCAACTGCATTCCAACACTTGACA
TGGAATATCCACGAGTGGGAAGTAACAGACTACGAAGAATAA

Upstream 100 bases:

>100_bases
AAAAATTGAAATTTGAGGAGGTGATTTCAAAAAGTAGTGAGATAAAATAAAGTGTCAAAATACGGACAGAACCAAAATAT
CAGATGAGGGGGATTTTTCA

Downstream 100 bases:

>100_bases
TTAGAAATAAGTAGCTAAGATAGCGAGTACAACCCCATCTGGGCATGTGCCACTCACATGCCCAGATTATTAAAACAATT
ATTCAGGCTAAAATAAAGGG

Product: extracellular solute-binding protein family 5

Products: ADP; phosphate; dipeptides [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 573; Mature: 572

Protein sequence:

>573_residues
MSSRKLFVLFLALMFATAVAIGGCAPPEEAKEEDPEAEEEEEAPAEVDNPAVERPNEIVIGGPDLEGIFNPVLYSGVYDS
WVLGMIFDSLLTVDENGQLTTDQRSVAKDYEISEDGTVYTFHLREGWEFHDGEEITAEDVAFSLEVTAHPDYDGPRSSFS
DDIVGVDEFREGETDELEGITVEDDYTLVVEAQEPSADNIFDFAVMIMPKHYYDFENYEDFQDLTDDPMGSGPFELVEYS
PDQHAILESFEDYYHGAPNVDRIIYEETETEQQIPMVETGEADIVQVSSTPENMEMLEDIDHQEALTFFDNAYTYIGLNH
EDEHLQHQEVRQALAYALDIEAFLDGMFGDELAQPIYTPFSPVSWAYPDEDDMNDYAYDPDKANELLDEAGYEWDDNEEY
RVNEDGERLSFTWETIADNEWSEHLTTLALEQWPQIGVELEIENYDFNTLTDRIDQDRGDVDMWNMGWSLSAEPDPSNIF
SVEYADAGLNYGMYHNEEAEELMQEGLETFDQDERAEVYNELGVLFNEDLPYIFVYSNKEIWSTNDRVENYEPTAFQHLT
WNIHEWEVTDYEE

Sequences:

>Translated_573_residues
MSSRKLFVLFLALMFATAVAIGGCAPPEEAKEEDPEAEEEEEAPAEVDNPAVERPNEIVIGGPDLEGIFNPVLYSGVYDS
WVLGMIFDSLLTVDENGQLTTDQRSVAKDYEISEDGTVYTFHLREGWEFHDGEEITAEDVAFSLEVTAHPDYDGPRSSFS
DDIVGVDEFREGETDELEGITVEDDYTLVVEAQEPSADNIFDFAVMIMPKHYYDFENYEDFQDLTDDPMGSGPFELVEYS
PDQHAILESFEDYYHGAPNVDRIIYEETETEQQIPMVETGEADIVQVSSTPENMEMLEDIDHQEALTFFDNAYTYIGLNH
EDEHLQHQEVRQALAYALDIEAFLDGMFGDELAQPIYTPFSPVSWAYPDEDDMNDYAYDPDKANELLDEAGYEWDDNEEY
RVNEDGERLSFTWETIADNEWSEHLTTLALEQWPQIGVELEIENYDFNTLTDRIDQDRGDVDMWNMGWSLSAEPDPSNIF
SVEYADAGLNYGMYHNEEAEELMQEGLETFDQDERAEVYNELGVLFNEDLPYIFVYSNKEIWSTNDRVENYEPTAFQHLT
WNIHEWEVTDYEE
>Mature_572_residues
SSRKLFVLFLALMFATAVAIGGCAPPEEAKEEDPEAEEEEEAPAEVDNPAVERPNEIVIGGPDLEGIFNPVLYSGVYDSW
VLGMIFDSLLTVDENGQLTTDQRSVAKDYEISEDGTVYTFHLREGWEFHDGEEITAEDVAFSLEVTAHPDYDGPRSSFSD
DIVGVDEFREGETDELEGITVEDDYTLVVEAQEPSADNIFDFAVMIMPKHYYDFENYEDFQDLTDDPMGSGPFELVEYSP
DQHAILESFEDYYHGAPNVDRIIYEETETEQQIPMVETGEADIVQVSSTPENMEMLEDIDHQEALTFFDNAYTYIGLNHE
DEHLQHQEVRQALAYALDIEAFLDGMFGDELAQPIYTPFSPVSWAYPDEDDMNDYAYDPDKANELLDEAGYEWDDNEEYR
VNEDGERLSFTWETIADNEWSEHLTTLALEQWPQIGVELEIENYDFNTLTDRIDQDRGDVDMWNMGWSLSAEPDPSNIFS
VEYADAGLNYGMYHNEEAEELMQEGLETFDQDERAEVYNELGVLFNEDLPYIFVYSNKEIWSTNDRVENYEPTAFQHLTW
NIHEWEVTDYEE

Specific function: This protein is a component of an oligopeptide permease, a binding protein-dependent transport system. This APP system can completely substitute for the OPP system in both sporulation and genetic competence. AppA can bind and transport tetra- and pentapep

COG id: COG0747

COG function: function code E; ABC-type dipeptide transport system, periplasmic component

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein 5 family [H]

Homologues:

Organism=Escherichia coli, GI1789966, Length=483, Percent_Identity=25.6728778467909, Blast_Score=135, Evalue=5e-33,
Organism=Escherichia coli, GI1787052, Length=465, Percent_Identity=24.0860215053763, Blast_Score=105, Evalue=6e-24,
Organism=Escherichia coli, GI1787762, Length=458, Percent_Identity=23.5807860262009, Blast_Score=93, Evalue=4e-20,
Organism=Escherichia coli, GI1787495, Length=489, Percent_Identity=22.4948875255624, Blast_Score=91, Evalue=2e-19,
Organism=Escherichia coli, GI1787551, Length=343, Percent_Identity=23.9067055393586, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1789397, Length=480, Percent_Identity=20.4166666666667, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: 660 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 2980 Molecules/Cell In: Growth Phase, Minimal Media (Based on E. coli). 40 Molecules/Cell In: Stationary Phase, Rich Media (Based on E. coli). [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000914 [H]

Pfam domain/function: PF00496 SBP_bac_5 [H]

EC number: NA

Molecular weight: Translated: 65940; Mature: 65809

Theoretical pI: Translated: 3.50; Mature: 3.50

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSRKLFVLFLALMFATAVAIGGCAPPEEAKEEDPEAEEEEEAPAEVDNPAVERPNEIVI
CCCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHCCCCCCCCCCCCCCCEEEE
GGPDLEGIFNPVLYSGVYDSWVLGMIFDSLLTVDENGQLTTDQRSVAKDYEISEDGTVYT
ECCCHHHHHHHHHHHCCHHHHHHHHHHHHHEEECCCCCEECCHHHHHHCCCCCCCCEEEE
FHLREGWEFHDGEEITAEDVAFSLEVTAHPDYDGPRSSFSDDIVGVDEFREGETDELEGI
EEECCCCCCCCCCCCCHHHEEEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCE
TVEDDYTLVVEAQEPSADNIFDFAVMIMPKHYYDFENYEDFQDLTDDPMGSGPFELVEYS
EECCCEEEEEEECCCCCCCHHEEHEEEECHHHCCCCCCCHHHHCCCCCCCCCCCEEEEEC
PDQHAILESFEDYYHGAPNVDRIIYEETETEQQIPMVETGEADIVQVSSTPENMEMLEDI
CCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHCCCEEECCCCCEEEECCCCHHHHHHHHC
DHQEALTFFDNAYTYIGLNHEDEHLQHQEVRQALAYALDIEAFLDGMFGDELAQPIYTPF
CHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCC
SPVSWAYPDEDDMNDYAYDPDKANELLDEAGYEWDDNEEYRVNEDGERLSFTWETIADNE
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEEEEECCCCC
WSEHLTTLALEQWPQIGVELEIENYDFNTLTDRIDQDRGDVDMWNMGWSLSAEPDPSNIF
HHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEECCEECCCCCCCCCEE
SVEYADAGLNYGMYHNEEAEELMQEGLETFDQDERAEVYNELGVLFNEDLPYIFVYSNKE
EEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCEEEEECCCE
IWSTNDRVENYEPTAFQHLTWNIHEWEVTDYEE
EECCCCCCCCCCCCEEEEEEEEEEEEEECCCCC
>Mature Secondary Structure 
SSRKLFVLFLALMFATAVAIGGCAPPEEAKEEDPEAEEEEEAPAEVDNPAVERPNEIVI
CCCHHHHHHHHHHHHHHHHHCCCCCCHHHHCCCCCCCHHHCCCCCCCCCCCCCCCEEEE
GGPDLEGIFNPVLYSGVYDSWVLGMIFDSLLTVDENGQLTTDQRSVAKDYEISEDGTVYT
ECCCHHHHHHHHHHHCCHHHHHHHHHHHHHEEECCCCCEECCHHHHHHCCCCCCCCEEEE
FHLREGWEFHDGEEITAEDVAFSLEVTAHPDYDGPRSSFSDDIVGVDEFREGETDELEGI
EEECCCCCCCCCCCCCHHHEEEEEEEEECCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCE
TVEDDYTLVVEAQEPSADNIFDFAVMIMPKHYYDFENYEDFQDLTDDPMGSGPFELVEYS
EECCCEEEEEEECCCCCCCHHEEHEEEECHHHCCCCCCCHHHHCCCCCCCCCCCEEEEEC
PDQHAILESFEDYYHGAPNVDRIIYEETETEQQIPMVETGEADIVQVSSTPENMEMLEDI
CCHHHHHHHHHHHHCCCCCCCEEEEECCCCHHCCCEEECCCCCEEEECCCCHHHHHHHHC
DHQEALTFFDNAYTYIGLNHEDEHLQHQEVRQALAYALDIEAFLDGMFGDELAQPIYTPF
CHHHHHHHHCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCC
SPVSWAYPDEDDMNDYAYDPDKANELLDEAGYEWDDNEEYRVNEDGERLSFTWETIADNE
CCCCCCCCCCCCCCCCCCCCHHHHHHHHHCCCCCCCCCCEEECCCCCEEEEEEEECCCCC
WSEHLTTLALEQWPQIGVELEIENYDFNTLTDRIDQDRGDVDMWNMGWSLSAEPDPSNIF
HHHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHCCCCCEEEEECCEECCCCCCCCCEE
SVEYADAGLNYGMYHNEEAEELMQEGLETFDQDERAEVYNELGVLFNEDLPYIFVYSNKE
EEEECCCCCCCCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHEECCCCCEEEEECCCE
IWSTNDRVENYEPTAFQHLTWNIHEWEVTDYEE
EECCCCCCCCCCCCEEEEEEEEEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; dipeptides [Periplasm]; H2O [C]

Specific reaction: ATP + dipeptides [Periplasm] + H2O = ADP + phosphate + dipeptides [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 7997159; 9384377 [H]