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The map label for this gene is grdE [H]

Identifier: 188585111

GI number: 188585111

Start: 500352

End: 501638

Strand: Direct

Name: grdE [H]

Synonym: Nther_0472

Alternate gene names: 188585111

Gene position: 500352-501638 (Clockwise)

Preceding gene: 188585110

Following gene: 188585112

Centisome position: 15.81

GC content: 40.02

Gene sequence:

>1287_bases
GTGAGCCTGAGGCTTGAAAAGGTTAATGTTAAAGACGTGAAGTTGGGTAGTGAAACAAGTTATAAAAATGGAGTAGTCAC
TGTTAATAAGGATGAATTGATAGAAGAATTAAAACAAGACGATAGAATTGGCTCAATAGAAATAGAAGTTGTTAAGCCAG
GGGAATCTGTTCGCATTATACCAGTTAAAGATGTTATAGAGCCTCGTATTAAAGTGGAGGGAGAAGGTGAATTATTCCCA
GGCTTACTAGGAAAAGTTTCTACTGTAGGAAGTGGTACAACCAAAACCTTTAATGGCATGAGTGTAGTGACTACAGGGAA
ATTGGTAGGTTTCCAAGAGGGAATTATAGACATGGATGGCCCAGGAGCTGACTACTCACCATTTTCTAAAACTCTAAACC
TTGTTGTGAACTGTGAGCCAGTCAAGGGCTTGAAGAGCCATGAACATGAAGAAGCTCTTCGTTTAGCTGGATTGAAAGCT
GCTCGCTATATTGCCGAAGCTTGTCAAGATGCACAAGCCGACGAACAAGAAGATTACGAAGCTTTGCCAGTGGAAGAAAT
GAAATCTAAGTATCCGAACTTGCCTAAAGTTGGGTATGTTTATATGCTTCAAAGTCAAGGTTTACTTCATGATACTTGGG
TGTATGGTATAGATGCAAAAGAAATTATTCCTACCTGCATCTATCCTACAGAAGTATTAGATGGGGCTATAGTTAGTGGT
AACTGTGTATCAGCCTGCGACAAAAATACTACTTACCATCATTTAAACAACCCAATAATTAAAGATCTATATGCCAGACA
TGGAAAGGATATAAATTTCTGTGGAGTAATTTTGACTAATGAAAACGTCACTTTGGCTGCTAAGGAACGTTCTTCAGGAT
ATACGGCTAAACTTGCTGAAATGTTAGGCCTTGACGGAGTCATTGTTTCTCAAGAAGGTTTTGGTAACCCCGATACAGAC
TTGATCATGAATTGTGTCAAGATTGAAGATAAGGGAATTAAGACAGTTTTAGTCACAGATGAGTATGCCGGTAGAGATGG
GGCGTCTCAGTCCTTGGCTGACGCAGACCCTAAAGCTGATGCAGTTGTTACTGCAGGTAATGCCAATGAAACCATAGTAC
TGCCTCCTATGGATAAAGTTGTTGGCATCCTGGAGACTGCAGATTCTATTGCTGGTGGTTTTGAAGGGAGCTTAAGACAC
GACAAAAGTATTGAGGTGGAATTACAAACAATCACAGGTGCTACAAATCAACTTGGATTTAACAAGCTCGGAGCTATAGA
AGCTTAA

Upstream 100 bases:

>100_bases
AGAAGATTTAGAAGCTGAAGAGATTGAAGATGAGGTAAAGAAGCATATTTAGTTGTTATGATTTTTGTGAAAAGTGAAGT
TAATCAAGGAGGTGAATTGT

Downstream 100 bases:

>100_bases
CTTTAAATTAACATATACCTGAATTTAAAGATATTACTATTAAGTGGAGGTGAGTTTATGTTTGAAGGTAAAAAAGTAGT
TATCTTAGGTGATCGCGATG

Product: Sarcosine reductase

Products: NA

Alternate protein names: Selenoprotein PB alpha/beta [H]

Number of amino acids: Translated: 428; Mature: 427

Protein sequence:

>428_residues
MSLRLEKVNVKDVKLGSETSYKNGVVTVNKDELIEELKQDDRIGSIEIEVVKPGESVRIIPVKDVIEPRIKVEGEGELFP
GLLGKVSTVGSGTTKTFNGMSVVTTGKLVGFQEGIIDMDGPGADYSPFSKTLNLVVNCEPVKGLKSHEHEEALRLAGLKA
ARYIAEACQDAQADEQEDYEALPVEEMKSKYPNLPKVGYVYMLQSQGLLHDTWVYGIDAKEIIPTCIYPTEVLDGAIVSG
NCVSACDKNTTYHHLNNPIIKDLYARHGKDINFCGVILTNENVTLAAKERSSGYTAKLAEMLGLDGVIVSQEGFGNPDTD
LIMNCVKIEDKGIKTVLVTDEYAGRDGASQSLADADPKADAVVTAGNANETIVLPPMDKVVGILETADSIAGGFEGSLRH
DKSIEVELQTITGATNQLGFNKLGAIEA

Sequences:

>Translated_428_residues
MSLRLEKVNVKDVKLGSETSYKNGVVTVNKDELIEELKQDDRIGSIEIEVVKPGESVRIIPVKDVIEPRIKVEGEGELFP
GLLGKVSTVGSGTTKTFNGMSVVTTGKLVGFQEGIIDMDGPGADYSPFSKTLNLVVNCEPVKGLKSHEHEEALRLAGLKA
ARYIAEACQDAQADEQEDYEALPVEEMKSKYPNLPKVGYVYMLQSQGLLHDTWVYGIDAKEIIPTCIYPTEVLDGAIVSG
NCVSACDKNTTYHHLNNPIIKDLYARHGKDINFCGVILTNENVTLAAKERSSGYTAKLAEMLGLDGVIVSQEGFGNPDTD
LIMNCVKIEDKGIKTVLVTDEYAGRDGASQSLADADPKADAVVTAGNANETIVLPPMDKVVGILETADSIAGGFEGSLRH
DKSIEVELQTITGATNQLGFNKLGAIEA
>Mature_427_residues
SLRLEKVNVKDVKLGSETSYKNGVVTVNKDELIEELKQDDRIGSIEIEVVKPGESVRIIPVKDVIEPRIKVEGEGELFPG
LLGKVSTVGSGTTKTFNGMSVVTTGKLVGFQEGIIDMDGPGADYSPFSKTLNLVVNCEPVKGLKSHEHEEALRLAGLKAA
RYIAEACQDAQADEQEDYEALPVEEMKSKYPNLPKVGYVYMLQSQGLLHDTWVYGIDAKEIIPTCIYPTEVLDGAIVSGN
CVSACDKNTTYHHLNNPIIKDLYARHGKDINFCGVILTNENVTLAAKERSSGYTAKLAEMLGLDGVIVSQEGFGNPDTDL
IMNCVKIEDKGIKTVLVTDEYAGRDGASQSLADADPKADAVVTAGNANETIVLPPMDKVVGILETADSIAGGFEGSLRHD
KSIEVELQTITGATNQLGFNKLGAIEA

Specific function: In the first step of glycine reductase, the substrate is bound to component PB via a Schiff base intermediate. Then the PB- activated substrate is nucleophilically attacked by the selenol anion of component PA to transform it to a carboxymethylated seleno

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016585
- InterPro:   IPR015417 [H]

Pfam domain/function: PF09338 Gly_reductase [H]

EC number: =1.21.4.2 [H]

Molecular weight: Translated: 46077; Mature: 45946

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLRLEKVNVKDVKLGSETSYKNGVVTVNKDELIEELKQDDRIGSIEIEVVKPGESVRII
CCEEEEECCCEEEECCCCCCCCCCEEEECHHHHHHHHHHCCCCCEEEEEEECCCCCEEEE
PVKDVIEPRIKVEGEGELFPGLLGKVSTVGSGTTKTFNGMSVVTTGKLVGFQEGIIDMDG
EHHHHCCCCEEECCCCCCCCHHHHHHHHCCCCCCEEECCEEEEEECEEEECCCCEEECCC
PGADYSPFSKTLNLVVNCEPVKGLKSHEHEEALRLAGLKAARYIAEACQDAQADEQEDYE
CCCCCCCHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ALPVEEMKSKYPNLPKVGYVYMLQSQGLLHDTWVYGIDAKEIIPTCIYPTEVLDGAIVSG
CCCHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEEECCHHHHCCCCCCHHHHHCCEEECC
NCVSACDKNTTYHHLNNPIIKDLYARHGKDINFCGVILTNENVTLAAKERSSGYTAKLAE
CCHHHCCCCCCEEECCCHHHHHHHHHCCCCCEEEEEEEECCCEEEEECCCCCCCHHHHHH
MLGLDGVIVSQEGFGNPDTDLIMNCVKIEDKGIKTVLVTDEYAGRDGASQSLADADPKAD
HHCCCCEEEECCCCCCCCHHHHEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
AVVTAGNANETIVLPPMDKVVGILETADSIAGGFEGSLRHDKSIEVELQTITGATNQLGF
EEEEECCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCCC
NKLGAIEA
CCCCCCCC
>Mature Secondary Structure 
SLRLEKVNVKDVKLGSETSYKNGVVTVNKDELIEELKQDDRIGSIEIEVVKPGESVRII
CEEEEECCCEEEECCCCCCCCCCEEEECHHHHHHHHHHCCCCCEEEEEEECCCCCEEEE
PVKDVIEPRIKVEGEGELFPGLLGKVSTVGSGTTKTFNGMSVVTTGKLVGFQEGIIDMDG
EHHHHCCCCEEECCCCCCCCHHHHHHHHCCCCCCEEECCEEEEEECEEEECCCCEEECCC
PGADYSPFSKTLNLVVNCEPVKGLKSHEHEEALRLAGLKAARYIAEACQDAQADEQEDYE
CCCCCCCHHHEEEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
ALPVEEMKSKYPNLPKVGYVYMLQSQGLLHDTWVYGIDAKEIIPTCIYPTEVLDGAIVSG
CCCHHHHHHHCCCCCCCEEEEEEECCCCEEEEEEEECCHHHHCCCCCCHHHHHCCEEECC
NCVSACDKNTTYHHLNNPIIKDLYARHGKDINFCGVILTNENVTLAAKERSSGYTAKLAE
CCHHHCCCCCCEEECCCHHHHHHHHHCCCCCEEEEEEEECCCEEEEECCCCCCCHHHHHH
MLGLDGVIVSQEGFGNPDTDLIMNCVKIEDKGIKTVLVTDEYAGRDGASQSLADADPKAD
HHCCCCEEEECCCCCCCCHHHHEEEEEECCCCCEEEEEECCCCCCCCCCCCCCCCCCCCC
AVVTAGNANETIVLPPMDKVVGILETADSIAGGFEGSLRHDKSIEVELQTITGATNQLGF
EEEEECCCCCEEEECCHHHHHHHHHHHHHHCCCCCCCCCCCCEEEEEEEEECCCCCCCCC
NKLGAIEA
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 10091582; 8529639 [H]