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The map label for this gene is gatB [H]

Identifier: 188585104

GI number: 188585104

Start: 492153

End: 493586

Strand: Direct

Name: gatB [H]

Synonym: Nther_0465

Alternate gene names: 188585104

Gene position: 492153-493586 (Clockwise)

Preceding gene: 188585103

Following gene: 188585106

Centisome position: 15.55

GC content: 37.87

Gene sequence:

>1434_bases
ATGGCAAATTACGAAACTGTTATTGGTCTCGAGGTGCATTCTGAATTATTAACATCAACCAAGATTTTCTGTGGATGTTC
AACTAGCTTTGGTGATGAGCCAAATTCAAATGTCTGTCCTATTTGTTTGGGAATGCCGGGCACTCTTCCTGTTCTAAATA
ACAGAGCATTGGAGCTAGCTGCTAAGGCAGGCCTGTCTCTGAATTGTGAAATTGCTGATTTTAGTAAATTTGACAGGAAA
AACTATTTTTATCCAGATCTACCAAAAGCTTATCAGATTTCACAATTTGACTTGCCCATCGCCAAAAACGGATATTTAGA
TGTAACAGTTAATGGCGAAAAGAAACGCATTAGAATTAACCGAATCCACATGGAAGAAGATGCCGGGAAACTTGTTCATT
CGGAAAGAGGAGGCGGCTCACTGGTTGATTTTAACAGAACTGGAGTACCTTTAATTGAAATCGTCACAGAACCCGATATA
GGTTCTCCCGAAGAAGCTAAAGCCTTTTTAGAAGGATTAAAAGCGATTTTACAGTATACAGATGTTTCGGACTGTAAGAT
GGAAGAAGGAAGCTTAAGATGTGATGCCAATGTGTCTCTAAGACCAGTGGGTAGTACAGAATTTGGTACAAAAACTGAAG
TTAAGAATATGAACTCCTTTAGAGCTGTAGAGAGGGCTCTTAACTATGAAGTGGAGCGACAAGCAGAAGCCCTTGAAGAT
GGTGAAGAAATTATCCAGGAGACTAGACGGTGGGACGAAGATCGCGGCATCACCATCTCCATGCGGTCTAAAGAAGAAGC
CCATGATTACAGATATTTCCCAGACCCTGATTTGGTTCCAGTAGTTATGAAAGAAGAACAGATCGAAAAAGTACGTCAGG
AAATTCCCGAACTTCCCAATGATAAGAAAGAAAGATTTATTAAAGACTTCAACTTATCAGAATATGATGCCTCAGTTATA
ACTGCCGATAAATCCTTGGCTGAATTCTTTGATGAATGTGTTAAAACTTATGATGAACCAAAGAAAATAGCTAATTGGAT
AATGGGTGAGATTTCTCGAGTTTTAAATGAAGACCAGATCGGAATTGAAGACACTAATATTTCTCCTGAAAGATTGACAG
AGCTTTTAAAGTTGGAAGATGAAGGAACTATCAGTAATAAAATCGCCAAGGAAGTATTTGAAGATATGTATCAAAATGAT
AAATCACCAAAGCAAATTGTAGAAGAAAAAGGCCTTGTGCAAATAAGTGATACATCTGAATTGGAAGGAATTATTGATCA
AGTAATTTCAGAAAACCCAAGTGCCGTAGAAGATTATCGCGGAGGAAAGAAAAAAGCTATGGGCTTTTTAGTGGGTCAGG
TTATGAAGAAAACGAAAGGAAAAGCTAATCCACAAAAAGTTAATGAATTATTACAGGAGAAATTGGAGAACTAG

Upstream 100 bases:

>100_bases
AGGGTAAGGTTATTCAAGTTGCTCATAAAATTGAACAAATGTTACAGGTGTTTAAAAACGCACCCCAACAGGGATTAGAT
AATCAAGGGGGGAAATAACG

Downstream 100 bases:

>100_bases
TCCATCTAATATAATGTGATCCAATGGCTATGACAAAAAGTTCTGCCCCTTGTTTGGGCAGAACTTTTTTAAGGTCTAAT
ATTAACTTAATACTCCCCAG

Product: aspartyl/glutamyl-tRNA(Asn/Gln) amidotransferase subunit B

Products: NA

Alternate protein names: Asp/Glu-ADT subunit B [H]

Number of amino acids: Translated: 477; Mature: 476

Protein sequence:

>477_residues
MANYETVIGLEVHSELLTSTKIFCGCSTSFGDEPNSNVCPICLGMPGTLPVLNNRALELAAKAGLSLNCEIADFSKFDRK
NYFYPDLPKAYQISQFDLPIAKNGYLDVTVNGEKKRIRINRIHMEEDAGKLVHSERGGGSLVDFNRTGVPLIEIVTEPDI
GSPEEAKAFLEGLKAILQYTDVSDCKMEEGSLRCDANVSLRPVGSTEFGTKTEVKNMNSFRAVERALNYEVERQAEALED
GEEIIQETRRWDEDRGITISMRSKEEAHDYRYFPDPDLVPVVMKEEQIEKVRQEIPELPNDKKERFIKDFNLSEYDASVI
TADKSLAEFFDECVKTYDEPKKIANWIMGEISRVLNEDQIGIEDTNISPERLTELLKLEDEGTISNKIAKEVFEDMYQND
KSPKQIVEEKGLVQISDTSELEGIIDQVISENPSAVEDYRGGKKKAMGFLVGQVMKKTKGKANPQKVNELLQEKLEN

Sequences:

>Translated_477_residues
MANYETVIGLEVHSELLTSTKIFCGCSTSFGDEPNSNVCPICLGMPGTLPVLNNRALELAAKAGLSLNCEIADFSKFDRK
NYFYPDLPKAYQISQFDLPIAKNGYLDVTVNGEKKRIRINRIHMEEDAGKLVHSERGGGSLVDFNRTGVPLIEIVTEPDI
GSPEEAKAFLEGLKAILQYTDVSDCKMEEGSLRCDANVSLRPVGSTEFGTKTEVKNMNSFRAVERALNYEVERQAEALED
GEEIIQETRRWDEDRGITISMRSKEEAHDYRYFPDPDLVPVVMKEEQIEKVRQEIPELPNDKKERFIKDFNLSEYDASVI
TADKSLAEFFDECVKTYDEPKKIANWIMGEISRVLNEDQIGIEDTNISPERLTELLKLEDEGTISNKIAKEVFEDMYQND
KSPKQIVEEKGLVQISDTSELEGIIDQVISENPSAVEDYRGGKKKAMGFLVGQVMKKTKGKANPQKVNELLQEKLEN
>Mature_476_residues
ANYETVIGLEVHSELLTSTKIFCGCSTSFGDEPNSNVCPICLGMPGTLPVLNNRALELAAKAGLSLNCEIADFSKFDRKN
YFYPDLPKAYQISQFDLPIAKNGYLDVTVNGEKKRIRINRIHMEEDAGKLVHSERGGGSLVDFNRTGVPLIEIVTEPDIG
SPEEAKAFLEGLKAILQYTDVSDCKMEEGSLRCDANVSLRPVGSTEFGTKTEVKNMNSFRAVERALNYEVERQAEALEDG
EEIIQETRRWDEDRGITISMRSKEEAHDYRYFPDPDLVPVVMKEEQIEKVRQEIPELPNDKKERFIKDFNLSEYDASVIT
ADKSLAEFFDECVKTYDEPKKIANWIMGEISRVLNEDQIGIEDTNISPERLTELLKLEDEGTISNKIAKEVFEDMYQNDK
SPKQIVEEKGLVQISDTSELEGIIDQVISENPSAVEDYRGGKKKAMGFLVGQVMKKTKGKANPQKVNELLQEKLEN

Specific function: Allows the formation of correctly charged Asn-tRNA(Asn) or Gln-tRNA(Gln) through the transamidation of misacylated Asp- tRNA(Asn) or Glu-tRNA(Gln) in organisms which lack either or both of asparaginyl-tRNA or glutaminyl-tRNA synthetases. The reaction take

COG id: COG0064

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase B subunit (PET112 homolog)

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the gatB/gatE family. GatB subfamily [H]

Homologues:

Organism=Homo sapiens, GI4758894, Length=494, Percent_Identity=38.663967611336, Blast_Score=350, Evalue=2e-96,
Organism=Caenorhabditis elegans, GI115534094, Length=471, Percent_Identity=32.0594479830149, Blast_Score=224, Evalue=1e-58,
Organism=Saccharomyces cerevisiae, GI6319391, Length=514, Percent_Identity=30.3501945525292, Blast_Score=226, Evalue=9e-60,
Organism=Drosophila melanogaster, GI45550808, Length=491, Percent_Identity=35.030549898167, Blast_Score=310, Evalue=2e-84,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR017959
- InterPro:   IPR018027
- InterPro:   IPR003789
- InterPro:   IPR023168
- InterPro:   IPR004413
- InterPro:   IPR006075
- InterPro:   IPR017958 [H]

Pfam domain/function: PF02934 GatB_N; PF02637 GatB_Yqey [H]

EC number: 6.3.5.-

Molecular weight: Translated: 53794; Mature: 53663

Theoretical pI: Translated: 4.45; Mature: 4.45

Prosite motif: PS01234 GATB

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.7 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANYETVIGLEVHSELLTSTKIFCGCSTSFGDEPNSNVCPICLGMPGTLPVLNNRALELA
CCCCCEEEEHHHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEECCCCCCCHHCCHHHHHH
AKAGLSLNCEIADFSKFDRKNYFYPDLPKAYQISQFDLPIAKNGYLDVTVNGEKKRIRIN
HHCCCEEEEEECCCHHHCCCCCCCCCCCCCEECCCCCCCEECCCEEEEEECCCCEEEEEE
RIHMEEDAGKLVHSERGGGSLVDFNRTGVPLIEIVTEPDIGSPEEAKAFLEGLKAILQYT
EEEEHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHC
DVSDCKMEEGSLRCDANVSLRPVGSTEFGTKTEVKNMNSFRAVERALNYEVERQAEALED
CCCCCCCCCCCEEECCCCEEEECCCCCCCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHH
GEEIIQETRRWDEDRGITISMRSKEEAHDYRYFPDPDLVPVVMKEEQIEKVRQEIPELPN
HHHHHHHHHCCCCCCCEEEEECCCHHHHCCCCCCCCCCEEHEECHHHHHHHHHHCCCCCC
DKKERFIKDFNLSEYDASVITADKSLAEFFDECVKTYDEPKKIANWIMGEISRVLNEDQI
HHHHHHHHHCCCCHHCCHHEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
GIEDTNISPERLTELLKLEDEGTISNKIAKEVFEDMYQNDKSPKQIVEEKGLVQISDTSE
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCHH
LEGIIDQVISENPSAVEDYRGGKKKAMGFLVGQVMKKTKGKANPQKVNELLQEKLEN
HHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
ANYETVIGLEVHSELLTSTKIFCGCSTSFGDEPNSNVCPICLGMPGTLPVLNNRALELA
CCCCEEEEHHHHHHHHHHHHEEEECCCCCCCCCCCCCCEEEECCCCCCCHHCCHHHHHH
AKAGLSLNCEIADFSKFDRKNYFYPDLPKAYQISQFDLPIAKNGYLDVTVNGEKKRIRIN
HHCCCEEEEEECCCHHHCCCCCCCCCCCCCEECCCCCCCEECCCEEEEEECCCCEEEEEE
RIHMEEDAGKLVHSERGGGSLVDFNRTGVPLIEIVTEPDIGSPEEAKAFLEGLKAILQYT
EEEEHHHHHHHHHCCCCCCCEEECCCCCCCEEEEEECCCCCCHHHHHHHHHHHHHHHHHC
DVSDCKMEEGSLRCDANVSLRPVGSTEFGTKTEVKNMNSFRAVERALNYEVERQAEALED
CCCCCCCCCCCEEECCCCEEEECCCCCCCCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHH
GEEIIQETRRWDEDRGITISMRSKEEAHDYRYFPDPDLVPVVMKEEQIEKVRQEIPELPN
HHHHHHHHHCCCCCCCEEEEECCCHHHHCCCCCCCCCCEEHEECHHHHHHHHHHCCCCCC
DKKERFIKDFNLSEYDASVITADKSLAEFFDECVKTYDEPKKIANWIMGEISRVLNEDQI
HHHHHHHHHCCCCHHCCHHEECHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHCCCCC
GIEDTNISPERLTELLKLEDEGTISNKIAKEVFEDMYQNDKSPKQIVEEKGLVQISDTSE
CCCCCCCCHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCHHHHHHHCCCEEECCCHH
LEGIIDQVISENPSAVEDYRGGKKKAMGFLVGQVMKKTKGKANPQKVNELLQEKLEN
HHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA