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The map label for this gene is ligA

Identifier: 188585101

GI number: 188585101

Start: 488294

End: 490285

Strand: Direct

Name: ligA

Synonym: Nther_0462

Alternate gene names: 188585101

Gene position: 488294-490285 (Clockwise)

Preceding gene: 188585100

Following gene: 188585102

Centisome position: 15.43

GC content: 37.6

Gene sequence:

>1992_bases
TTGAGTAAAGATATTCAAAAGAAAGTTGAAGCTCTGAGAGAAAAAATTGAATATCACAATCATCGTTATTACGTTTTGGA
CGCCCCTGAGATATCTGATCAAGAATTTGATGCTTTAATGAATGAATTAATAGAGCTTGAGCAAACTTATCCGGAATATC
AAAGCCCTGATTCTCCTTCACAAAGAGTAGGTGGAGAACCCTTGGATAAATTCCCTCGGGTAAGTCACGAAATACCCATG
TTGAGCTTGGAAAATGCCGAGAGCCAAAAAGGATTACTAGATTTCCACCAAAGAGTTAGCAAAAATACCCAAAAAACAGA
CCCGGTATATGTGGCAGAATTAAAAATTGATGGTTTGGCTCTTTCGTTAAGATATGAACAGGGGATTTTAGTAAGAGGAG
CAACCAGGGGCGATGGGACAACTGGCGAAGATATTACTCCTAATGTCAAAACAGTGAGATCCATACCTTTAAAGTTATCT
AAACCCCTAGATATTGAAATCAGGGGTGAGGCTTTTATGTCTAAAGCTTCTTTTGAGCGTTTGAATCAGGAAAAGGCAGA
ACGAGGCGAAGATCCATTTGCCAATCCCCGAAATGCTGCAGCCGGTTCTTTAAGACAACTAGATCCTAAAATACCGGCCA
AAAGAGACTTAGACTTTTTCCCCTACAGTGTTCCATATATAAGGGGTGAAAATTTAGATAGTCATTATTCTGCGGTAAAA
GAATTGAAGGATCTCGGTTTTAAAATAAATCCTTACATCAGGAAATTTGAAACCATGGAAGAAGTTATCTCTTATTGTGA
AGAATGGCAGGAAAAGCGGACAAAACTTCCCTATGAAATTGATGGAGTTGTTATTAAATTAAACGATTATAACTTACAAC
AACAATTGGGTGCCACTTCTAAAAACCCCAGATGGGCCATAGCTTATAAATTTCCTGCTGAACAAGCTGAGAGTCAAGTG
AATAATATTTTTATTAATGTAGGTAGAACTGGAGCCTTAACCCCTGTAGTGGAACTTGAACCTGTAAGGATTGCGGGTTC
TACAGTCAAACGAGCTAGCTTGCATAATGAAGATATCCTCAGACAAAAAGATGTCAGGATAGGTGATCGGGTCATCATTC
AAAAAGCCGGAGATATAATTCCAGAAGTGGTAAAAGTTAAAGAGGAAGCGCGAACTGGTCACGAACAACCATTTGTCTAT
CCTGAGTCTTGTCCAGTTTGCAAAAGTGAAGCCAAAAGAATCAATGATGAAGCTATACTTAGGTGCATAAATCCTGGCTG
TCCTGCCCAAGCAAAAGAAAGAATAATACATTTTTCATCTAGAGATGCTATGGATATTGAAGGATTAGGAGAAAAAGTTG
TAGAAAAATTATATTCTCACGGTTTAATAAAGGACGTGGCGGATATTTACTATTTGGCTAAAAATGAATTATCGAACTTG
GAAGGTTTCGGGGACAAGTCTGCAGAAAACTTGTTGCAAGCTATAGAGGAAAGTAAAAAAAATCCATTTAACAAGTTATT
ATACGGCCTGGGGATAAGGTTAGTAGGTAAAAGAGCAGCCCAATTACTGGCATTTGAATTTGAGCATCTGGACAATTTGA
TGAAAGCACAAATAGAGGATCTGACTAAAATAAATGATATTGGACCTAGGATGGCTACCAGTATAGTAAGCTTTTTTCAA
TTAGAACATACTCACAATTTAATCAGAAAACTTAAAAAAGCTGGAGTCAATATGAAAGAACCCACGGAACAAAACAAGAG
TAGTGATCCATCTCTAACTGGTAAGTTAGTAGTGATTACGGGGACTTTTGATAATTACACCAGAAGGGAATTGACTGATT
TGATTGAAGCAAAAGGTGCTAAAGTGACCAGTAATGTCAGTAGTAATACAGATTTTGTATTAGTGGGAGCAAACCCCGGT
TCAAAGCGTGACAAAGCGCAAGACCTGGGATTAACAATTATAGAAGAAAGTGATTTAGAAGATTTTTTGTAA

Upstream 100 bases:

>100_bases
GGTAGTGATTGGGTTTTGACAGTTTCCTTTGACGGTGCCGGCATTAAAAAACTCGCTGCTAGTGTAGCACCTATTAAGAA
ACTGAAATGAGGTGTGGCAT

Downstream 100 bases:

>100_bases
AGGTATGACTGTAAGCCAGATAAGATAGAATATATAAAATTATTCAAACCAAATACTTGTTGTCTGATTAGGAGGTGAAT
TTTCATGAAGGTTAGTAAAG

Product: DNA ligase, NAD-dependent

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+]

Number of amino acids: Translated: 663; Mature: 662

Protein sequence:

>663_residues
MSKDIQKKVEALREKIEYHNHRYYVLDAPEISDQEFDALMNELIELEQTYPEYQSPDSPSQRVGGEPLDKFPRVSHEIPM
LSLENAESQKGLLDFHQRVSKNTQKTDPVYVAELKIDGLALSLRYEQGILVRGATRGDGTTGEDITPNVKTVRSIPLKLS
KPLDIEIRGEAFMSKASFERLNQEKAERGEDPFANPRNAAAGSLRQLDPKIPAKRDLDFFPYSVPYIRGENLDSHYSAVK
ELKDLGFKINPYIRKFETMEEVISYCEEWQEKRTKLPYEIDGVVIKLNDYNLQQQLGATSKNPRWAIAYKFPAEQAESQV
NNIFINVGRTGALTPVVELEPVRIAGSTVKRASLHNEDILRQKDVRIGDRVIIQKAGDIIPEVVKVKEEARTGHEQPFVY
PESCPVCKSEAKRINDEAILRCINPGCPAQAKERIIHFSSRDAMDIEGLGEKVVEKLYSHGLIKDVADIYYLAKNELSNL
EGFGDKSAENLLQAIEESKKNPFNKLLYGLGIRLVGKRAAQLLAFEFEHLDNLMKAQIEDLTKINDIGPRMATSIVSFFQ
LEHTHNLIRKLKKAGVNMKEPTEQNKSSDPSLTGKLVVITGTFDNYTRRELTDLIEAKGAKVTSNVSSNTDFVLVGANPG
SKRDKAQDLGLTIIEESDLEDFL

Sequences:

>Translated_663_residues
MSKDIQKKVEALREKIEYHNHRYYVLDAPEISDQEFDALMNELIELEQTYPEYQSPDSPSQRVGGEPLDKFPRVSHEIPM
LSLENAESQKGLLDFHQRVSKNTQKTDPVYVAELKIDGLALSLRYEQGILVRGATRGDGTTGEDITPNVKTVRSIPLKLS
KPLDIEIRGEAFMSKASFERLNQEKAERGEDPFANPRNAAAGSLRQLDPKIPAKRDLDFFPYSVPYIRGENLDSHYSAVK
ELKDLGFKINPYIRKFETMEEVISYCEEWQEKRTKLPYEIDGVVIKLNDYNLQQQLGATSKNPRWAIAYKFPAEQAESQV
NNIFINVGRTGALTPVVELEPVRIAGSTVKRASLHNEDILRQKDVRIGDRVIIQKAGDIIPEVVKVKEEARTGHEQPFVY
PESCPVCKSEAKRINDEAILRCINPGCPAQAKERIIHFSSRDAMDIEGLGEKVVEKLYSHGLIKDVADIYYLAKNELSNL
EGFGDKSAENLLQAIEESKKNPFNKLLYGLGIRLVGKRAAQLLAFEFEHLDNLMKAQIEDLTKINDIGPRMATSIVSFFQ
LEHTHNLIRKLKKAGVNMKEPTEQNKSSDPSLTGKLVVITGTFDNYTRRELTDLIEAKGAKVTSNVSSNTDFVLVGANPG
SKRDKAQDLGLTIIEESDLEDFL
>Mature_662_residues
SKDIQKKVEALREKIEYHNHRYYVLDAPEISDQEFDALMNELIELEQTYPEYQSPDSPSQRVGGEPLDKFPRVSHEIPML
SLENAESQKGLLDFHQRVSKNTQKTDPVYVAELKIDGLALSLRYEQGILVRGATRGDGTTGEDITPNVKTVRSIPLKLSK
PLDIEIRGEAFMSKASFERLNQEKAERGEDPFANPRNAAAGSLRQLDPKIPAKRDLDFFPYSVPYIRGENLDSHYSAVKE
LKDLGFKINPYIRKFETMEEVISYCEEWQEKRTKLPYEIDGVVIKLNDYNLQQQLGATSKNPRWAIAYKFPAEQAESQVN
NIFINVGRTGALTPVVELEPVRIAGSTVKRASLHNEDILRQKDVRIGDRVIIQKAGDIIPEVVKVKEEARTGHEQPFVYP
ESCPVCKSEAKRINDEAILRCINPGCPAQAKERIIHFSSRDAMDIEGLGEKVVEKLYSHGLIKDVADIYYLAKNELSNLE
GFGDKSAENLLQAIEESKKNPFNKLLYGLGIRLVGKRAAQLLAFEFEHLDNLMKAQIEDLTKINDIGPRMATSIVSFFQL
EHTHNLIRKLKKAGVNMKEPTEQNKSSDPSLTGKLVVITGTFDNYTRRELTDLIEAKGAKVTSNVSSNTDFVLVGANPGS
KRDKAQDLGLTIIEESDLEDFL

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain

Homologues:

Organism=Escherichia coli, GI1788750, Length=668, Percent_Identity=44.0119760479042, Blast_Score=575, Evalue=1e-165,
Organism=Escherichia coli, GI87082305, Length=523, Percent_Identity=23.5181644359465, Blast_Score=117, Evalue=3e-27,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): DNLJ_NATTJ (B2A5W5)

Other databases:

- EMBL:   CP001034
- RefSeq:   YP_001916646.1
- ProteinModelPortal:   B2A5W5
- GeneID:   6315340
- GenomeReviews:   CP001034_GR
- KEGG:   nth:Nther_0462
- HOGENOM:   HBG620317
- OMA:   IKHFASR
- GO:   GO:0005622
- HAMAP:   MF_01588
- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994
- InterPro:   IPR004149
- Gene3D:   G3DSA:2.40.50.140
- PIRSF:   PIRSF001604
- SMART:   SM00292
- SMART:   SM00278
- SMART:   SM00532
- TIGRFAMs:   TIGR00575

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB; PF03119 DNA_ligase_ZBD; SSF52113 BRCT; SSF50249 Nucleic_acid_OB; SSF47781 RuvA_2_like

EC number: =6.5.1.2

Molecular weight: Translated: 74762; Mature: 74631

Theoretical pI: Translated: 5.64; Mature: 5.64

Prosite motif: PS50172 BRCT; PS01055 DNA_LIGASE_N1; PS01056 DNA_LIGASE_N2

Important sites: ACT_SITE 115-115 BINDING 113-113 BINDING 136-136 BINDING 170-170 BINDING 286-286 BINDING 310-310

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSKDIQKKVEALREKIEYHNHRYYVLDAPEISDQEFDALMNELIELEQTYPEYQSPDSPS
CCHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCH
QRVGGEPLDKFPRVSHEIPMLSLENAESQKGLLDFHQRVSKNTQKTDPVYVAELKIDGLA
HHCCCCCHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCEE
LSLRYEQGILVRGATRGDGTTGEDITPNVKTVRSIPLKLSKPLDIEIRGEAFMSKASFER
EEEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHH
LNQEKAERGEDPFANPRNAAAGSLRQLDPKIPAKRDLDFFPYSVPYIRGENLDSHYSAVK
HHHHHHHCCCCCCCCCCCHHHCCHHHCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHH
ELKDLGFKINPYIRKFETMEEVISYCEEWQEKRTKLPYEIDGVVIKLNDYNLQQQLGATS
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEECCCHHHHHCCCC
KNPRWAIAYKFPAEQAESQVNNIFINVGRTGALTPVVELEPVRIAGSTVKRASLHNEDIL
CCCCEEEEEECCHHHHHHHHCEEEEECCCCCCCCCEEEECCEEECCCHHHHHHCCCHHHH
RQKDVRIGDRVIIQKAGDIIPEVVKVKEEARTGHEQPFVYPESCPVCKSEAKRINDEAIL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHCCHHHHH
RCINPGCPAQAKERIIHFSSRDAMDIEGLGEKVVEKLYSHGLIKDVADIYYLAKNELSNL
HCCCCCCCHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
EGFGDKSAENLLQAIEESKKNPFNKLLYGLGIRLVGKRAAQLLAFEFEHLDNLMKAQIED
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTKINDIGPRMATSIVSFFQLEHTHNLIRKLKKAGVNMKEPTEQNKSSDPSLTGKLVVIT
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEE
GTFDNYTRRELTDLIEAKGAKVTSNVSSNTDFVLVGANPGSKRDKAQDLGLTIIEESDLE
ECCCCHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCCCCCHHHHCCEEEEECCCHH
DFL
HCC
>Mature Secondary Structure 
SKDIQKKVEALREKIEYHNHRYYVLDAPEISDQEFDALMNELIELEQTYPEYQSPDSPS
CHHHHHHHHHHHHHHHHCCCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCH
QRVGGEPLDKFPRVSHEIPMLSLENAESQKGLLDFHQRVSKNTQKTDPVYVAELKIDGLA
HHCCCCCHHHCCCCCCCCCEEEECCCCCHHHHHHHHHHHHCCCCCCCCEEEEEEEECCEE
LSLRYEQGILVRGATRGDGTTGEDITPNVKTVRSIPLKLSKPLDIEIRGEAFMSKASFER
EEEEECCCEEEEECCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCEEECCHHHHHHHHHHH
LNQEKAERGEDPFANPRNAAAGSLRQLDPKIPAKRDLDFFPYSVPYIRGENLDSHYSAVK
HHHHHHHCCCCCCCCCCCHHHCCHHHCCCCCCCCCCCCCCCCCCCEEECCCCCHHHHHHH
ELKDLGFKINPYIRKFETMEEVISYCEEWQEKRTKLPYEIDGVVIKLNDYNLQQQLGATS
HHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEECCEEEEEECCCHHHHHCCCC
KNPRWAIAYKFPAEQAESQVNNIFINVGRTGALTPVVELEPVRIAGSTVKRASLHNEDIL
CCCCEEEEEECCHHHHHHHHCEEEEECCCCCCCCCEEEECCEEECCCHHHHHHCCCHHHH
RQKDVRIGDRVIIQKAGDIIPEVVKVKEEARTGHEQPFVYPESCPVCKSEAKRINDEAIL
HHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCHHHHHHCCHHHHH
RCINPGCPAQAKERIIHFSSRDAMDIEGLGEKVVEKLYSHGLIKDVADIYYLAKNELSNL
HCCCCCCCHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHC
EGFGDKSAENLLQAIEESKKNPFNKLLYGLGIRLVGKRAAQLLAFEFEHLDNLMKAQIED
CCCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LTKINDIGPRMATSIVSFFQLEHTHNLIRKLKKAGVNMKEPTEQNKSSDPSLTGKLVVIT
HHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCEEEEEEE
GTFDNYTRRELTDLIEAKGAKVTSNVSSNTDFVLVGANPGSKRDKAQDLGLTIIEESDLE
ECCCCHHHHHHHHHHHHCCCEEECCCCCCCCEEEEECCCCCCCCHHHHCCEEEEECCCHH
DFL
HCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA