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The map label for this gene is pcrA [H]

Identifier: 188585100

GI number: 188585100

Start: 486103

End: 488283

Strand: Direct

Name: pcrA [H]

Synonym: Nther_0461

Alternate gene names: 188585100

Gene position: 486103-488283 (Clockwise)

Preceding gene: 188585099

Following gene: 188585101

Centisome position: 15.36

GC content: 37.87

Gene sequence:

>2181_bases
ATGGTGGTATCATCACAATTAGCAGAATTAAATCAAGAGCAAACAGCTGCTGTCACCGCCGGTGATGGCCCTGTTTTAGT
ACTTGCAGGAGCGGGTAGTGGAAAAACAAAAGTTTTAACATATAGAATTGCTTATTTATTAGAAACAAAGGGCATTCACC
CTGAACAAATTTTAGCCCTTACATTTACTAATAAAGCTGCTGGTGAAATGAAAGAGCGAGTGCAGACTATCGTAGAAGGT
GATGCACCTTCTTGGGTATCGACCTTTCATGCAGCTTGTGTTAGGATATTGCGACGTGAAATTGATCTACTGGGTTATGG
AAAGGATTTTTTGATCTACGATACTAGGGATAAGACCAAAATAGTCAAGGATTTGTTAGCTGACTTTAATTTGGATAAAA
AGAAATATCCTCATCAGGCTGTAGCTAAAAAGATAAGTGGTGTAAAAAATAATCTACAGGATCCTAGAGAATTATTTTAT
CCCTATGATGAGGTTTTTGAAGCGTATCAGAAACGATTAAAAAAAGAGAATGCCTTAGATTTTGATGATTTACTTCTATT
GACATTAAGATTGTTTGAACAATACCCTGATCGATTACAATACTATCAAAATAAATTCCAATATATTTTGGTTGACGAAT
TTCAAGATACTAACCCTGCTCAGAATAGGTTGATTTCTTTATTATCGCCACCTCAGGATAATGTTTTTGTCGTGGGCGAT
GATGATCAGTCAATATATTTGTTCAGAGGGGCCGATGTTAAAAATATCTTGGAATTTGAAAAAACTTATCCCAATGCTCA
GGTTTACAAATTGGAAAAGAATTATCGGTCAAGCAAGACTATTTTGGAAGCGGCCAATCAAGTAGTTACAAATAATCCTA
GTCGTAAATCCAAAGAATTGTGGACTGACAATGATGAGGGTTATCCTTTGCAGTATTACGAAGCTGATGACGAAAGAGAT
GAAGCATACTTTATCGCTGAAGAAATCCGTGCCAATAGATATCAACTGCCTCTAATAGCAGTACTTTACAGAACCAATGC
CCAGTCCAGGGTATTGGAAGATATTTTTAGAAAAATGGGGATCTCTTATGAAGTTGTAGGAGGGACTGGATTTTACGACC
GTAAAGAGGTAAAAGATATAATGGCTTATTTAATGGTTGTTCAGTGTCCACAATCTGATAGCCAACTAGAGAGAATTATT
AACGAGCCAAAAAGAGGAATTGGAAAGACCAGCGTAGATAAATTGAAGGACTATGCTAATAGAAACAATATTACTTTATT
TGATGCCCTTTTAGAAGTAGACTATACCGATATTAGTAAACGAGGAAAAAATTCTGTTAAAAAGTTTACTGATATGATTG
GTAATTTTCGAAAGATGAGGGAATACTTGACTGTAAGGGAACTAATCGAAGAAGTTGCTGAAAAATCCGGTTATCTGGAA
GCACTTAGATCTCAAAATACCAAAGAAGCTGAAACTCGAATGGAAAACGTGAGAGAAATTTATTCTATGGCCAAAGAATT
TGAAGCAGAAGGAGGGGAAACCCTTCAGGAATTTTTGACCCACAGTACTTTAATGGGTGATATAGATACATGGGAAGATG
AGGAACAAGCACAAGTTGTGATGATGACTCTTCACAGTGCCAAGGGACTTGAGTTTCCAGGGGTATTTTTGACAGGTTTA
GAGGAAGGAGTTTTTCCTCATATTCGTACCTATGACAGTGAAGCGTCTATGGAAGAAGAGCGTCGCCTTTGCTATGTAGG
AATTACCAGAGCTGAACAGGTGTTGTACTTAACCTGGGCTAGAGAGAGAACCCTTAACGGTATGACAAGAATGTTACAAC
CATCCAGATTTGTTTCGGAAATAGGAGAGGACTTAAAGGATACTCAACATCAGACACTATCGGGACAAGGACAAGTAACT
AGCACTCAAAATTCCCATGAGCCACCACAAACTCAAATTTCTTCTTTTTCAGATAAAACTAGTCCTTCCGGGAAGAGAAA
TGTTAACTTAGCGGAAGAAAGTTATCAGGAAGGAGACTTGATTTCCCACAAAAAATGGGGAGAAGGAAAGGTGTTGCAAG
CCCAGCAAGCTGGTAGTGATTGGGTTTTGACAGTTTCCTTTGACGGTGCCGGCATTAAAAAACTCGCTGCTAGTGTAGCA
CCTATTAAGAAACTGAAATGA

Upstream 100 bases:

>100_bases
AAAACTTAACCAAAAAGAACAGTAAGTAACGCTGACATTAGGAAGAGTAGTCCGAATATTTTGGATTACTCTTCCTAATT
TCGTTAAGGAAGGGTGGTAA

Downstream 100 bases:

>100_bases
GGTGTGGCATTTGAGTAAAGATATTCAAAAGAAAGTTGAAGCTCTGAGAGAAAAAATTGAATATCACAATCATCGTTATT
ACGTTTTGGACGCCCCTGAG

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 726; Mature: 726

Protein sequence:

>726_residues
MVVSSQLAELNQEQTAAVTAGDGPVLVLAGAGSGKTKVLTYRIAYLLETKGIHPEQILALTFTNKAAGEMKERVQTIVEG
DAPSWVSTFHAACVRILRREIDLLGYGKDFLIYDTRDKTKIVKDLLADFNLDKKKYPHQAVAKKISGVKNNLQDPRELFY
PYDEVFEAYQKRLKKENALDFDDLLLLTLRLFEQYPDRLQYYQNKFQYILVDEFQDTNPAQNRLISLLSPPQDNVFVVGD
DDQSIYLFRGADVKNILEFEKTYPNAQVYKLEKNYRSSKTILEAANQVVTNNPSRKSKELWTDNDEGYPLQYYEADDERD
EAYFIAEEIRANRYQLPLIAVLYRTNAQSRVLEDIFRKMGISYEVVGGTGFYDRKEVKDIMAYLMVVQCPQSDSQLERII
NEPKRGIGKTSVDKLKDYANRNNITLFDALLEVDYTDISKRGKNSVKKFTDMIGNFRKMREYLTVRELIEEVAEKSGYLE
ALRSQNTKEAETRMENVREIYSMAKEFEAEGGETLQEFLTHSTLMGDIDTWEDEEQAQVVMMTLHSAKGLEFPGVFLTGL
EEGVFPHIRTYDSEASMEEERRLCYVGITRAEQVLYLTWARERTLNGMTRMLQPSRFVSEIGEDLKDTQHQTLSGQGQVT
STQNSHEPPQTQISSFSDKTSPSGKRNVNLAEESYQEGDLISHKKWGEGKVLQAQQAGSDWVLTVSFDGAGIKKLAASVA
PIKKLK

Sequences:

>Translated_726_residues
MVVSSQLAELNQEQTAAVTAGDGPVLVLAGAGSGKTKVLTYRIAYLLETKGIHPEQILALTFTNKAAGEMKERVQTIVEG
DAPSWVSTFHAACVRILRREIDLLGYGKDFLIYDTRDKTKIVKDLLADFNLDKKKYPHQAVAKKISGVKNNLQDPRELFY
PYDEVFEAYQKRLKKENALDFDDLLLLTLRLFEQYPDRLQYYQNKFQYILVDEFQDTNPAQNRLISLLSPPQDNVFVVGD
DDQSIYLFRGADVKNILEFEKTYPNAQVYKLEKNYRSSKTILEAANQVVTNNPSRKSKELWTDNDEGYPLQYYEADDERD
EAYFIAEEIRANRYQLPLIAVLYRTNAQSRVLEDIFRKMGISYEVVGGTGFYDRKEVKDIMAYLMVVQCPQSDSQLERII
NEPKRGIGKTSVDKLKDYANRNNITLFDALLEVDYTDISKRGKNSVKKFTDMIGNFRKMREYLTVRELIEEVAEKSGYLE
ALRSQNTKEAETRMENVREIYSMAKEFEAEGGETLQEFLTHSTLMGDIDTWEDEEQAQVVMMTLHSAKGLEFPGVFLTGL
EEGVFPHIRTYDSEASMEEERRLCYVGITRAEQVLYLTWARERTLNGMTRMLQPSRFVSEIGEDLKDTQHQTLSGQGQVT
STQNSHEPPQTQISSFSDKTSPSGKRNVNLAEESYQEGDLISHKKWGEGKVLQAQQAGSDWVLTVSFDGAGIKKLAASVA
PIKKLK
>Mature_726_residues
MVVSSQLAELNQEQTAAVTAGDGPVLVLAGAGSGKTKVLTYRIAYLLETKGIHPEQILALTFTNKAAGEMKERVQTIVEG
DAPSWVSTFHAACVRILRREIDLLGYGKDFLIYDTRDKTKIVKDLLADFNLDKKKYPHQAVAKKISGVKNNLQDPRELFY
PYDEVFEAYQKRLKKENALDFDDLLLLTLRLFEQYPDRLQYYQNKFQYILVDEFQDTNPAQNRLISLLSPPQDNVFVVGD
DDQSIYLFRGADVKNILEFEKTYPNAQVYKLEKNYRSSKTILEAANQVVTNNPSRKSKELWTDNDEGYPLQYYEADDERD
EAYFIAEEIRANRYQLPLIAVLYRTNAQSRVLEDIFRKMGISYEVVGGTGFYDRKEVKDIMAYLMVVQCPQSDSQLERII
NEPKRGIGKTSVDKLKDYANRNNITLFDALLEVDYTDISKRGKNSVKKFTDMIGNFRKMREYLTVRELIEEVAEKSGYLE
ALRSQNTKEAETRMENVREIYSMAKEFEAEGGETLQEFLTHSTLMGDIDTWEDEEQAQVVMMTLHSAKGLEFPGVFLTGL
EEGVFPHIRTYDSEASMEEERRLCYVGITRAEQVLYLTWARERTLNGMTRMLQPSRFVSEIGEDLKDTQHQTLSGQGQVT
STQNSHEPPQTQISSFSDKTSPSGKRNVNLAEESYQEGDLISHKKWGEGKVLQAQQAGSDWVLTVSFDGAGIKKLAASVA
PIKKLK

Specific function: DNA helicase used for plasmid rolling-circle replication and also involved in UV repair [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI2367296, Length=744, Percent_Identity=36.9623655913978, Blast_Score=464, Evalue=1e-131,
Organism=Escherichia coli, GI48994965, Length=639, Percent_Identity=40.0625978090767, Blast_Score=398, Evalue=1e-112,
Organism=Escherichia coli, GI1787196, Length=138, Percent_Identity=32.6086956521739, Blast_Score=79, Evalue=1e-15,
Organism=Saccharomyces cerevisiae, GI6322369, Length=761, Percent_Identity=27.4638633377135, Blast_Score=226, Evalue=8e-60,
Organism=Saccharomyces cerevisiae, GI6324477, Length=668, Percent_Identity=24.8502994011976, Blast_Score=145, Evalue=2e-35,

Paralogues:

None

Copy number: 3000 [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005751
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 82888; Mature: 82888

Theoretical pI: Translated: 5.02; Mature: 5.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVVSSQLAELNQEQTAAVTAGDGPVLVLAGAGSGKTKVLTYRIAYLLETKGIHPEQILAL
CCCCHHHHHCCCHHHEEEEECCCCEEEEEECCCCCCEEHHHHHHHHHHCCCCCHHHEEEE
TFTNKAAGEMKERVQTIVEGDAPSWVSTFHAACVRILRREIDLLGYGKDFLIYDTRDKTK
EECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHH
IVKDLLADFNLDKKKYPHQAVAKKISGVKNNLQDPRELFYPYDEVFEAYQKRLKKENALD
HHHHHHHHCCCCHHCCCHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHCCCCC
FDDLLLLTLRLFEQYPDRLQYYQNKFQYILVDEFQDTNPAQNRLISLLSPPQDNVFVVGD
HHHHHHHHHHHHHHCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEC
DDQSIYLFRGADVKNILEFEKTYPNAQVYKLEKNYRSSKTILEAANQVVTNNPSRKSKEL
CCCEEEEEECCCHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHHHHHCCCCCCCCCCC
WTDNDEGYPLQYYEADDERDEAYFIAEEIRANRYQLPLIAVLYRTNAQSRVLEDIFRKMG
CCCCCCCCCEEEEECCCCCCHHEEEHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHC
ISYEVVGGTGFYDRKEVKDIMAYLMVVQCPQSDSQLERIINEPKRGIGKTSVDKLKDYAN
CCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHC
RNNITLFDALLEVDYTDISKRGKNSVKKFTDMIGNFRKMREYLTVRELIEEVAEKSGYLE
CCCEEEEHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALRSQNTKEAETRMENVREIYSMAKEFEAEGGETLQEFLTHSTLMGDIDTWEDEEQAQVV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
MMTLHSAKGLEFPGVFLTGLEEGVFPHIRTYDSEASMEEERRLCYVGITRAEQVLYLTWA
HEEHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHCCEEEEEECCCCEEEEEEEH
RERTLNGMTRMLQPSRFVSEIGEDLKDTQHQTLSGQGQVTSTQNSHEPPQTQISSFSDKT
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHCCCCC
SPSGKRNVNLAEESYQEGDLISHKKWGEGKVLQAQQAGSDWVLTVSFDGAGIKKLAASVA
CCCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEECCCCHHHHHHHHH
PIKKLK
HHHHCC
>Mature Secondary Structure
MVVSSQLAELNQEQTAAVTAGDGPVLVLAGAGSGKTKVLTYRIAYLLETKGIHPEQILAL
CCCCHHHHHCCCHHHEEEEECCCCEEEEEECCCCCCEEHHHHHHHHHHCCCCCHHHEEEE
TFTNKAAGEMKERVQTIVEGDAPSWVSTFHAACVRILRREIDLLGYGKDFLIYDTRDKTK
EECCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHH
IVKDLLADFNLDKKKYPHQAVAKKISGVKNNLQDPRELFYPYDEVFEAYQKRLKKENALD
HHHHHHHHCCCCHHCCCHHHHHHHHHHHHHCCCCHHHHCCCHHHHHHHHHHHHHHCCCCC
FDDLLLLTLRLFEQYPDRLQYYQNKFQYILVDEFQDTNPAQNRLISLLSPPQDNVFVVGD
HHHHHHHHHHHHHHCHHHHHHHHCCCEEEEEECCCCCCHHHHHHHHHCCCCCCCEEEEEC
DDQSIYLFRGADVKNILEFEKTYPNAQVYKLEKNYRSSKTILEAANQVVTNNPSRKSKEL
CCCEEEEEECCCHHHHHHHHHCCCCCEEEEEHHHCCHHHHHHHHHHHHHCCCCCCCCCCC
WTDNDEGYPLQYYEADDERDEAYFIAEEIRANRYQLPLIAVLYRTNAQSRVLEDIFRKMG
CCCCCCCCCEEEEECCCCCCHHEEEHHHHHCCCCCCCCEEEEECCCCHHHHHHHHHHHHC
ISYEVVGGTGFYDRKEVKDIMAYLMVVQCPQSDSQLERIINEPKRGIGKTSVDKLKDYAN
CCEEEECCCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHHC
RNNITLFDALLEVDYTDISKRGKNSVKKFTDMIGNFRKMREYLTVRELIEEVAEKSGYLE
CCCEEEEHHHHHCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALRSQNTKEAETRMENVREIYSMAKEFEAEGGETLQEFLTHSTLMGDIDTWEDEEQAQVV
HHHCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCCCCHHHHHH
MMTLHSAKGLEFPGVFLTGLEEGVFPHIRTYDSEASMEEERRLCYVGITRAEQVLYLTWA
HEEHHCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCHHHHCCEEEEEECCCCEEEEEEEH
RERTLNGMTRMLQPSRFVSEIGEDLKDTQHQTLSGQGQVTSTQNSHEPPQTQISSFSDKT
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCCEECCCCCCCCCHHHHHHCCCCC
SPSGKRNVNLAEESYQEGDLISHKKWGEGKVLQAQQAGSDWVLTVSFDGAGIKKLAASVA
CCCCCCCCCCHHHHCCCCCCCCCCCCCCCCEEECCCCCCCEEEEEEECCCCHHHHHHHHH
PIKKLK
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9701819; 9384377 [H]