Click here to switch to the map view.

The map label for this gene is purD [H]

Identifier: 188585098

GI number: 188585098

Start: 484373

End: 485662

Strand: Direct

Name: purD [H]

Synonym: Nther_0459

Alternate gene names: 188585098

Gene position: 484373-485662 (Clockwise)

Preceding gene: 188585097

Following gene: 188585099

Centisome position: 15.3

GC content: 38.91

Gene sequence:

>1290_bases
GTGAAAGTTCTGGTAATTGGTTCAGGAGGGCGTGAACATGCCCTGGTTAAAAAAATAGCAGAAAGTAGTCGGGTGACTGA
TTTATATGTAGCTCCTGGGAATCACGGTATAGCGAAAGATGCGGAGTGTATTGATATTAAGGAAACTGACATTAACAAAC
TAGCTGATTTAGCAATCCAAAAAGATATAGATTTAACGATTGTGGGACCAGAACAACCCCTTTCACAAGGAATAGTGGAT
CATTTTCAAAGTAAAGGTTTGAATATCTTTGGACCAAGTCAAAAAGCAGCTAAAATTGAGACTAGTAAATGGTTTGCTAA
ACAATTAATGCAGACATATGGGATTCCTACGGGGAAAGCTGAATATATTGAAGGTGATGCAGGGCTAGACAATTTAGAAA
AAGGTTTATCTGCACTGGAGGATATGTCACCTCCTTTTGTGGTGAAAGCTGATGGATTATATGCCGGAAAAGGTGTGATT
GTAACTGAAGAAATTTCGGAAGCTGAACAGGCAGTGGAAAACTTTTTGTCAGAACATACCAGTATATTAATCGAGGAATA
TCTAGAAGGAGAAGAACTGTCTCTATTAGCTTTTAGTGATGGCCATAAAGTACTTCCATTAATACCTAGCCAGGATTATA
AAAGAGCTTATGACAATGATCAAGGTCCTAATACGGGAGGAATGGGTTCTTATGCCCCTGTACCATCAATTTCTCAGGAA
CAGCTAGAATGGATTGTTGATAACATTATGCAGCCAACTATTACAGCTTTAAAGAAACAAGGTGTTACATACCAAGGTAT
TCTGTACGCAGGTTTAATTTTGACGGATTCCGGGCCAAAAGTATTAGAGTTTAATGCCAGGTTTGGAGACCCTGAAACCC
AGGCAATTTTGCCATTACTAAAAACGGACATATTAGAACCTATAATGGGGGTAGTAGCTGGAAATTTAGAAACTGAATTA
AAGGAGCCTTTGAAGGATGCATTGTCCTGGAAAGCTGAAAAAGCTGTTTGTGTAGTACTAGCTTCAGGGGGATATCCTAA
AGAGTATCAAAAAGGTTTTCCTATAAAAATCCCTCAAGAATTATTGACTTGGAATAATGTTAATCTTTATTACGCCGGTG
TAGCCAGTGAAAATGGAACACCTGTAACTGCCGGAGGCAGAGTTTTGAATGTGACGGGTATTGGTGAATCATGGAAGGAA
GCCCGAGAAAATGCATACGAAGGCGTAAGTACCGTTGAATTTGATGGAAAACATTTCCGCAATGATATTGCCCTACAGAC
TTTAAAATGA

Upstream 100 bases:

>100_bases
GATTCAATTGATCTTGCTCAAGAAACAGGAATCACCATGTATTTTACAGGTATACGTCATTTTAAACATTAATTTTAAAC
TGAAAGCTGGGGGGTTTAAG

Downstream 100 bases:

>100_bases
TAAAATACCAGTGGGACAACCAGGTAATATTTATCAGGGAAGAATTTCCTATTTTCTTCGGGGGTGATGTTATCCGAAAT
GTACAGGACGATTACATGGA

Product: phosphoribosylamine--glycine ligase

Products: NA

Alternate protein names: GARS; Glycinamide ribonucleotide synthetase; Phosphoribosylglycinamide synthetase [H]

Number of amino acids: Translated: 429; Mature: 429

Protein sequence:

>429_residues
MKVLVIGSGGREHALVKKIAESSRVTDLYVAPGNHGIAKDAECIDIKETDINKLADLAIQKDIDLTIVGPEQPLSQGIVD
HFQSKGLNIFGPSQKAAKIETSKWFAKQLMQTYGIPTGKAEYIEGDAGLDNLEKGLSALEDMSPPFVVKADGLYAGKGVI
VTEEISEAEQAVENFLSEHTSILIEEYLEGEELSLLAFSDGHKVLPLIPSQDYKRAYDNDQGPNTGGMGSYAPVPSISQE
QLEWIVDNIMQPTITALKKQGVTYQGILYAGLILTDSGPKVLEFNARFGDPETQAILPLLKTDILEPIMGVVAGNLETEL
KEPLKDALSWKAEKAVCVVLASGGYPKEYQKGFPIKIPQELLTWNNVNLYYAGVASENGTPVTAGGRVLNVTGIGESWKE
ARENAYEGVSTVEFDGKHFRNDIALQTLK

Sequences:

>Translated_429_residues
MKVLVIGSGGREHALVKKIAESSRVTDLYVAPGNHGIAKDAECIDIKETDINKLADLAIQKDIDLTIVGPEQPLSQGIVD
HFQSKGLNIFGPSQKAAKIETSKWFAKQLMQTYGIPTGKAEYIEGDAGLDNLEKGLSALEDMSPPFVVKADGLYAGKGVI
VTEEISEAEQAVENFLSEHTSILIEEYLEGEELSLLAFSDGHKVLPLIPSQDYKRAYDNDQGPNTGGMGSYAPVPSISQE
QLEWIVDNIMQPTITALKKQGVTYQGILYAGLILTDSGPKVLEFNARFGDPETQAILPLLKTDILEPIMGVVAGNLETEL
KEPLKDALSWKAEKAVCVVLASGGYPKEYQKGFPIKIPQELLTWNNVNLYYAGVASENGTPVTAGGRVLNVTGIGESWKE
ARENAYEGVSTVEFDGKHFRNDIALQTLK
>Mature_429_residues
MKVLVIGSGGREHALVKKIAESSRVTDLYVAPGNHGIAKDAECIDIKETDINKLADLAIQKDIDLTIVGPEQPLSQGIVD
HFQSKGLNIFGPSQKAAKIETSKWFAKQLMQTYGIPTGKAEYIEGDAGLDNLEKGLSALEDMSPPFVVKADGLYAGKGVI
VTEEISEAEQAVENFLSEHTSILIEEYLEGEELSLLAFSDGHKVLPLIPSQDYKRAYDNDQGPNTGGMGSYAPVPSISQE
QLEWIVDNIMQPTITALKKQGVTYQGILYAGLILTDSGPKVLEFNARFGDPETQAILPLLKTDILEPIMGVVAGNLETEL
KEPLKDALSWKAEKAVCVVLASGGYPKEYQKGFPIKIPQELLTWNNVNLYYAGVASENGTPVTAGGRVLNVTGIGESWKE
ARENAYEGVSTVEFDGKHFRNDIALQTLK

Specific function: De novo purine biosynthesis; second step. [C]

COG id: COG0151

COG function: function code F; Phosphoribosylamine-glycine ligase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ATP-grasp domain [H]

Homologues:

Organism=Homo sapiens, GI28416899, Length=436, Percent_Identity=43.8073394495413, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI4503915, Length=436, Percent_Identity=43.8073394495413, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI209869995, Length=436, Percent_Identity=43.8073394495413, Blast_Score=340, Evalue=1e-93,
Organism=Homo sapiens, GI209869993, Length=436, Percent_Identity=43.8073394495413, Blast_Score=340, Evalue=1e-93,
Organism=Escherichia coli, GI1790438, Length=440, Percent_Identity=42.7272727272727, Blast_Score=327, Evalue=1e-90,
Organism=Caenorhabditis elegans, GI17567511, Length=435, Percent_Identity=41.6091954022989, Blast_Score=293, Evalue=1e-79,
Organism=Saccharomyces cerevisiae, GI6321203, Length=453, Percent_Identity=37.7483443708609, Blast_Score=288, Evalue=1e-78,
Organism=Drosophila melanogaster, GI62471619, Length=450, Percent_Identity=40.6666666666667, Blast_Score=289, Evalue=2e-78,
Organism=Drosophila melanogaster, GI24582400, Length=450, Percent_Identity=40.6666666666667, Blast_Score=288, Evalue=3e-78,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR020561
- InterPro:   IPR000115
- InterPro:   IPR020560
- InterPro:   IPR020559
- InterPro:   IPR020562
- InterPro:   IPR011054 [H]

Pfam domain/function: PF01071 GARS_A; PF02843 GARS_C; PF02844 GARS_N [H]

EC number: =6.3.4.13 [H]

Molecular weight: Translated: 46594; Mature: 46594

Theoretical pI: Translated: 4.48; Mature: 4.48

Prosite motif: PS50975 ATP_GRASP ; PS00184 GARS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
1.9 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
1.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKVLVIGSGGREHALVKKIAESSRVTDLYVAPGNHGIAKDAECIDIKETDINKLADLAIQ
CEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEECCCCCHHHHHHHHHH
KDIDLTIVGPEQPLSQGIVDHFQSKGLNIFGPSQKAAKIETSKWFAKQLMQTYGIPTGKA
CCCCEEEECCCCCHHHHHHHHHHHCCCEEECCCCCHHEEHHHHHHHHHHHHHHCCCCCCC
EYIEGDAGLDNLEKGLSALEDMSPPFVVKADGLYAGKGVIVTEEISEAEQAVENFLSEHT
CEEECCCCHHHHHHHHHHHHHCCCCEEEEECCEEECCCEEEEHHHHHHHHHHHHHHHHHH
SILIEEYLEGEELSLLAFSDGHKVLPLIPSQDYKRAYDNDQGPNTGGMGSYAPVPSISQE
HHHHHHHCCCCCEEEEEECCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
QLEWIVDNIMQPTITALKKQGVTYQGILYAGLILTDSGPKVLEFNARFGDPETQAILPLL
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHH
KTDILEPIMGVVAGNLETELKEPLKDALSWKAEKAVCVVLASGGYPKEYQKGFPIKIPQE
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHCCCCCCCCHH
LLTWNNVNLYYAGVASENGTPVTAGGRVLNVTGIGESWKEARENAYEGVSTVEFDGKHFR
HHCCCCCEEEEEEEECCCCCEEECCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCHHHH
NDIALQTLK
CCCEEEECC
>Mature Secondary Structure
MKVLVIGSGGREHALVKKIAESSRVTDLYVAPGNHGIAKDAECIDIKETDINKLADLAIQ
CEEEEECCCCCHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCEEECCCCCHHHHHHHHHH
KDIDLTIVGPEQPLSQGIVDHFQSKGLNIFGPSQKAAKIETSKWFAKQLMQTYGIPTGKA
CCCCEEEECCCCCHHHHHHHHHHHCCCEEECCCCCHHEEHHHHHHHHHHHHHHCCCCCCC
EYIEGDAGLDNLEKGLSALEDMSPPFVVKADGLYAGKGVIVTEEISEAEQAVENFLSEHT
CEEECCCCHHHHHHHHHHHHHCCCCEEEEECCEEECCCEEEEHHHHHHHHHHHHHHHHHH
SILIEEYLEGEELSLLAFSDGHKVLPLIPSQDYKRAYDNDQGPNTGGMGSYAPVPSISQE
HHHHHHHCCCCCEEEEEECCCCEEEEECCCCHHHHHCCCCCCCCCCCCCCCCCCCCCCHH
QLEWIVDNIMQPTITALKKQGVTYQGILYAGLILTDSGPKVLEFNARFGDPETQAILPLL
HHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEEECCCCCEEEEECCCCCCHHHHHHHHH
KTDILEPIMGVVAGNLETELKEPLKDALSWKAEKAVCVVLASGGYPKEYQKGFPIKIPQE
HHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCEEEEEEECCCCCHHHHCCCCCCCCHH
LLTWNNVNLYYAGVASENGTPVTAGGRVLNVTGIGESWKEARENAYEGVSTVEFDGKHFR
HHCCCCCEEEEEEEECCCCCEEECCCEEEEEECCCHHHHHHHHHHHCCCEEEEECCHHHH
NDIALQTLK
CCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]