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The map label for this gene is asnB [H]

Identifier: 188585083

GI number: 188585083

Start: 467489

End: 468937

Strand: Direct

Name: asnB [H]

Synonym: Nther_0444

Alternate gene names: 188585083

Gene position: 467489-468937 (Clockwise)

Preceding gene: 188585082

Following gene: 188585084

Centisome position: 14.77

GC content: 38.1

Gene sequence:

>1449_bases
ATGAGCGGTATTGTAGCTTTACAAGGTGATTTGAAAGATTCTGAGAAGTTAGAGACCATGTTGAGTAAATTAGAACACAG
AGGGCCTGATGCTAGGGAAACTTATACCATGGCCGATTCTTTCCTAGGACAGGTCAAAAGCCAAGGGGAGCCTTCTTTTA
CAAAAAAAAGAGCAGCCACCCTGGTTTTAGATGGTCAGCCAAGTTATCTAGGGAAACAAATCAGCAGAGAAGAGCTGTTA
AATCTTTATCTAGAAAAAGGTACCAAGTTTATCAAACAATTGGGTGATGCCTTTACCTTTATTCTTTCAGATGGAGATGA
ACTAGTGGCTGCAAGGGACACCTTTGGTGCTAGGCCGTTATACTATGTAAACTGTGAAAATGGCTTAATGTTTGCTTCAG
AAATTAAAGCTTTAGAAGAGTTGAACCCGGATGCAGAAATCAAAATCTTTCCACCAGGGACTTACTATTCTTCAACTGAG
GGTTTCTCAACTTTTAAAAAGATACCTGGTTTCCAAGCAAATATACACGCTTCTCCAGGAAGCCAGCGCTATAACCAGAA
AGCTGAAGAATTAAATAGATTACTTATTGAAGCAGTTAGAAAAAATGTAACAGATCAAGAGAATATTGGTGTATTTCTAA
GCGGGGGAGTAGATAGCTCCATTATTGCAGCGGCCCTTGATAAGATTACTGATAAACCAATTAAAACTTTTGCAGTTGGT
GTTGCAGGTAGCGATGATGTGATGAAAGCCCGTAAAGTTGCCGAATTTTTAAATAGCGAGCATAAAGAATACACATATGA
TTATGACGACATGATGAAAGTGCTTCCGGAAGTAATATATTATCTAGAATCCTTTGATGTAGAATTGGTCAATAGTTCAA
TTGCCAATTTTCTAGTTGCATACTTAGCTAAGGAATCCGGAATGGAAGTAGTGCTGTCAGGGGAAGGAGCGGATGAACTC
TTTGGTGGGTATCACCATCTTAAGGATTGTTCAACGGACAGTGAATTGAACAGGGAATTGGAACATTTGTTGAAGGGACT
TCACAATGGAGGGCTACAGCGGGTAGATAGAATGACAAAGGCCCATGCTTTAGACTGTCGTATGCCTTTTTTTGATAAAG
ATGTTATAGATTTTGCTAGGGTGATACCCTGTAACTGGAAAATTACTGATGAAGGTATGGAAAAACTCATTTTAAGGCAG
GCCTTTGATGGCATGTTACCAGAAGAGGTACTATGGCGTAAAAAAGTACAATTTGGAATAGGATCTGGCAATGAAGGTGT
TATGGAGGAGAAGATTAAAAACCAAATTACAGATAAGGAATATAGAAATGCCCAGAAGAATACGAGCTTTACTTTTAAAT
CTAAAATCGAATATTACTATTATAAAATCTTTAAAGAAAAACATCCATTAAAAGGAATAGAAAGTACCGTTAATAGATGG
CTTGCGTAG

Upstream 100 bases:

>100_bases
GTCAACTTGAGTTGTTTACTGCATATGGTATGATAGAGAGTGACTAGCACAAAATTAAAAATCCTTTAACGAACTCTAAA
AGAGGTGATAATTTAATTTT

Downstream 100 bases:

>100_bases
AATTGTGTTATTTAACAATATTAAAAAAAGGCTGTATAAAAGTTAATATTAATGCTGTAAAATTTTAATTTTACAGCATT
AACAACTAAATATAAGCGCT

Product: asparagine synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 482; Mature: 481

Protein sequence:

>482_residues
MSGIVALQGDLKDSEKLETMLSKLEHRGPDARETYTMADSFLGQVKSQGEPSFTKKRAATLVLDGQPSYLGKQISREELL
NLYLEKGTKFIKQLGDAFTFILSDGDELVAARDTFGARPLYYVNCENGLMFASEIKALEELNPDAEIKIFPPGTYYSSTE
GFSTFKKIPGFQANIHASPGSQRYNQKAEELNRLLIEAVRKNVTDQENIGVFLSGGVDSSIIAAALDKITDKPIKTFAVG
VAGSDDVMKARKVAEFLNSEHKEYTYDYDDMMKVLPEVIYYLESFDVELVNSSIANFLVAYLAKESGMEVVLSGEGADEL
FGGYHHLKDCSTDSELNRELEHLLKGLHNGGLQRVDRMTKAHALDCRMPFFDKDVIDFARVIPCNWKITDEGMEKLILRQ
AFDGMLPEEVLWRKKVQFGIGSGNEGVMEEKIKNQITDKEYRNAQKNTSFTFKSKIEYYYYKIFKEKHPLKGIESTVNRW
LA

Sequences:

>Translated_482_residues
MSGIVALQGDLKDSEKLETMLSKLEHRGPDARETYTMADSFLGQVKSQGEPSFTKKRAATLVLDGQPSYLGKQISREELL
NLYLEKGTKFIKQLGDAFTFILSDGDELVAARDTFGARPLYYVNCENGLMFASEIKALEELNPDAEIKIFPPGTYYSSTE
GFSTFKKIPGFQANIHASPGSQRYNQKAEELNRLLIEAVRKNVTDQENIGVFLSGGVDSSIIAAALDKITDKPIKTFAVG
VAGSDDVMKARKVAEFLNSEHKEYTYDYDDMMKVLPEVIYYLESFDVELVNSSIANFLVAYLAKESGMEVVLSGEGADEL
FGGYHHLKDCSTDSELNRELEHLLKGLHNGGLQRVDRMTKAHALDCRMPFFDKDVIDFARVIPCNWKITDEGMEKLILRQ
AFDGMLPEEVLWRKKVQFGIGSGNEGVMEEKIKNQITDKEYRNAQKNTSFTFKSKIEYYYYKIFKEKHPLKGIESTVNRW
LA
>Mature_481_residues
SGIVALQGDLKDSEKLETMLSKLEHRGPDARETYTMADSFLGQVKSQGEPSFTKKRAATLVLDGQPSYLGKQISREELLN
LYLEKGTKFIKQLGDAFTFILSDGDELVAARDTFGARPLYYVNCENGLMFASEIKALEELNPDAEIKIFPPGTYYSSTEG
FSTFKKIPGFQANIHASPGSQRYNQKAEELNRLLIEAVRKNVTDQENIGVFLSGGVDSSIIAAALDKITDKPIKTFAVGV
AGSDDVMKARKVAEFLNSEHKEYTYDYDDMMKVLPEVIYYLESFDVELVNSSIANFLVAYLAKESGMEVVLSGEGADELF
GGYHHLKDCSTDSELNRELEHLLKGLHNGGLQRVDRMTKAHALDCRMPFFDKDVIDFARVIPCNWKITDEGMEKLILRQA
FDGMLPEEVLWRKKVQFGIGSGNEGVMEEKIKNQITDKEYRNAQKNTSFTFKSKIEYYYYKIFKEKHPLKGIESTVNRWL
A

Specific function: Asparagine biosynthesis. [C]

COG id: COG0367

COG function: function code E; Asparagine synthase (glutamine-hydrolyzing)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 glutamine amidotransferase type-2 domain [H]

Homologues:

Organism=Homo sapiens, GI168229248, Length=535, Percent_Identity=31.214953271028, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI168229252, Length=535, Percent_Identity=31.214953271028, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI168229250, Length=535, Percent_Identity=31.214953271028, Blast_Score=188, Evalue=1e-47,
Organism=Homo sapiens, GI296010852, Length=434, Percent_Identity=32.7188940092166, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI296010850, Length=434, Percent_Identity=32.7188940092166, Blast_Score=186, Evalue=3e-47,
Organism=Homo sapiens, GI296010848, Length=434, Percent_Identity=32.7188940092166, Blast_Score=186, Evalue=6e-47,
Organism=Escherichia coli, GI1786889, Length=519, Percent_Identity=30.2504816955684, Blast_Score=206, Evalue=3e-54,
Organism=Caenorhabditis elegans, GI17560178, Length=401, Percent_Identity=33.6658354114713, Blast_Score=211, Evalue=9e-55,
Organism=Caenorhabditis elegans, GI71993933, Length=430, Percent_Identity=31.8604651162791, Blast_Score=210, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI25147557, Length=430, Percent_Identity=31.8604651162791, Blast_Score=209, Evalue=2e-54,
Organism=Caenorhabditis elegans, GI25147560, Length=178, Percent_Identity=32.0224719101124, Blast_Score=96, Evalue=5e-20,
Organism=Saccharomyces cerevisiae, GI6321563, Length=464, Percent_Identity=32.3275862068966, Blast_Score=206, Evalue=8e-54,
Organism=Saccharomyces cerevisiae, GI6325403, Length=466, Percent_Identity=30.4721030042918, Blast_Score=189, Evalue=6e-49,
Organism=Drosophila melanogaster, GI45553209, Length=507, Percent_Identity=29.9802761341223, Blast_Score=199, Evalue=3e-51,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006426
- InterPro:   IPR001962
- InterPro:   IPR017932
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00733 Asn_synthase [H]

EC number: =6.3.5.4 [H]

Molecular weight: Translated: 54377; Mature: 54246

Theoretical pI: Translated: 5.22; Mature: 5.22

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGIVALQGDLKDSEKLETMLSKLEHRGPDARETYTMADSFLGQVKSQGEPSFTKKRAAT
CCCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCEE
LVLDGQPSYLGKQISREELLNLYLEKGTKFIKQLGDAFTFILSDGDELVAARDTFGARPL
EEECCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEHHCCCCCCE
YYVNCENGLMFASEIKALEELNPDAEIKIFPPGTYYSSTEGFSTFKKIPGFQANIHASPG
EEEECCCCCEEHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHCCCCCEEEECCCC
SQRYNQKAEELNRLLIEAVRKNVTDQENIGVFLSGGVDSSIIAAALDKITDKPIKTFAVG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCHHEEEE
VAGSDDVMKARKVAEFLNSEHKEYTYDYDDMMKVLPEVIYYLESFDVELVNSSIANFLVA
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
YLAKESGMEVVLSGEGADELFGGYHHLKDCSTDSELNRELEHLLKGLHNGGLQRVDRMTK
HHHHCCCCEEEEECCCHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
AHALDCRMPFFDKDVIDFARVIPCNWKITDEGMEKLILRQAFDGMLPEEVLWRKKVQFGI
HHHHHCCCCCCCHHHHHHHHHCCCCCEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
GSGNEGVMEEKIKNQITDKEYRNAQKNTSFTFKSKIEYYYYKIFKEKHPLKGIESTVNRW
CCCCCCHHHHHHHHHCCCHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LA
CC
>Mature Secondary Structure 
SGIVALQGDLKDSEKLETMLSKLEHRGPDARETYTMADSFLGQVKSQGEPSFTKKRAAT
CCEEEECCCCCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHCCCCCCHHHHHCEE
LVLDGQPSYLGKQISREELLNLYLEKGTKFIKQLGDAFTFILSDGDELVAARDTFGARPL
EEECCCCHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHEEEECCCCCEEEEHHCCCCCCE
YYVNCENGLMFASEIKALEELNPDAEIKIFPPGTYYSSTEGFSTFKKIPGFQANIHASPG
EEEECCCCCEEHHHHHHHHHCCCCCEEEEECCCCCCCCCCCHHHHHHCCCCCEEEECCCC
SQRYNQKAEELNRLLIEAVRKNVTDQENIGVFLSGGVDSSIIAAALDKITDKPIKTFAVG
HHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCHHHHHHHHHHHCCCCCHHEEEE
VAGSDDVMKARKVAEFLNSEHKEYTYDYDDMMKVLPEVIYYLESFDVELVNSSIANFLVA
CCCCHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHH
YLAKESGMEVVLSGEGADELFGGYHHLKDCSTDSELNRELEHLLKGLHNGGLQRVDRMTK
HHHHCCCCEEEEECCCHHHHHCCHHHHHCCCCHHHHHHHHHHHHHHHHCCHHHHHHHHHH
AHALDCRMPFFDKDVIDFARVIPCNWKITDEGMEKLILRQAFDGMLPEEVLWRKKVQFGI
HHHHHCCCCCCCHHHHHHHHHCCCCCEECHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
GSGNEGVMEEKIKNQITDKEYRNAQKNTSFTFKSKIEYYYYKIFKEKHPLKGIESTVNRW
CCCCCCHHHHHHHHHCCCHHHHCCCCCCCEEHHHHHHHHHHHHHHHCCCCCHHHHHHHHH
LA
CC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 1973930; 8905232; 9278503; 10587437 [H]