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The map label for this gene is yhfI [H]

Identifier: 188585080

GI number: 188585080

Start: 464405

End: 465184

Strand: Direct

Name: yhfI [H]

Synonym: Nther_0441

Alternate gene names: 188585080

Gene position: 464405-465184 (Clockwise)

Preceding gene: 188585079

Following gene: 188585081

Centisome position: 14.67

GC content: 38.33

Gene sequence:

>780_bases
ATGAAAATGACCAAAGCTTTTATGCTTAGAGTATTGGGTAGATATGCTCCCTTTCCTGCTAAAAATAGTAACTGTTCAGG
TTATCTACTAACAGCGGGGGAGGATAACTTTTTATTTGATTGTGGTAATGGGGTTTTGAGTAATTTGCAGAACTATCTGG
ATCTATCTCAATTATCCGCTTTATTTATAACTCATCATCACAGTGATCATGTTGCAGACTTAATAGCACTGCGACATGCC
TATGAAGACCTGCAAAGCAGAGGGGAAGTAACAAGCAAATTGCCGGTATACACTCCCCCTTACCCCCAAGATACAGTGGC
TGAATTAGAAGAATATCCTGGCTTTGATATTAAGTTTTTAGCTGAGGGAGAAGATATTATTATAGAAGATAAAAAAATTT
CTTGGTCTGTCACAAACCATTTTTTATATTGTGCGGCTTACCGTGTTGAATACAAAGAAAAAGCTTTTGTGTATACAGGG
GATAATGCTTATGACAACGAAACTATTGAAGATAACTCAACTGCAGACATTATTAACTTGTCAAAAGGAGCGGATTTGCT
GCTAACTGAAGCAAGCCTAGAAGAAGAATGGAAAAAAGAACCAGAAAAGAGACATATGAGTGCAAGAGATGCCGCCAGTT
TAGCCAAAGAGGCCCAGGTAAAGCATATGGTGATATCGCATTTCCATCCAAGGGCTGATTTGAGAAAGCTATTGCGGGAA
TCTAAACAGATACATCCTAATACTTCTCTGGCTGTAGAAGGAACGTCATATTTAGTATGA

Upstream 100 bases:

>100_bases
AAAGTTTATTTATCTAGGGTTGGACTGTTGAGATTTTCTATAGGAATTATTAGGCTTGGTATTAAATTATTATTGTCTAA
ACTAACAGGAGTGTGATTAC

Downstream 100 bases:

>100_bases
TTTTCTGTCCATTTTTTCTGGGGAAGTGATTTTATGGAAATCATTGATTTTAAACAAAGTAATCTGAATCGGGTTTACAA
ATATAATGAGGAGTTAAAAA

Product: beta-lactamase domain protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 259; Mature: 259

Protein sequence:

>259_residues
MKMTKAFMLRVLGRYAPFPAKNSNCSGYLLTAGEDNFLFDCGNGVLSNLQNYLDLSQLSALFITHHHSDHVADLIALRHA
YEDLQSRGEVTSKLPVYTPPYPQDTVAELEEYPGFDIKFLAEGEDIIIEDKKISWSVTNHFLYCAAYRVEYKEKAFVYTG
DNAYDNETIEDNSTADIINLSKGADLLLTEASLEEEWKKEPEKRHMSARDAASLAKEAQVKHMVISHFHPRADLRKLLRE
SKQIHPNTSLAVEGTSYLV

Sequences:

>Translated_259_residues
MKMTKAFMLRVLGRYAPFPAKNSNCSGYLLTAGEDNFLFDCGNGVLSNLQNYLDLSQLSALFITHHHSDHVADLIALRHA
YEDLQSRGEVTSKLPVYTPPYPQDTVAELEEYPGFDIKFLAEGEDIIIEDKKISWSVTNHFLYCAAYRVEYKEKAFVYTG
DNAYDNETIEDNSTADIINLSKGADLLLTEASLEEEWKKEPEKRHMSARDAASLAKEAQVKHMVISHFHPRADLRKLLRE
SKQIHPNTSLAVEGTSYLV
>Mature_259_residues
MKMTKAFMLRVLGRYAPFPAKNSNCSGYLLTAGEDNFLFDCGNGVLSNLQNYLDLSQLSALFITHHHSDHVADLIALRHA
YEDLQSRGEVTSKLPVYTPPYPQDTVAELEEYPGFDIKFLAEGEDIIIEDKKISWSVTNHFLYCAAYRVEYKEKAFVYTG
DNAYDNETIEDNSTADIINLSKGADLLLTEASLEEEWKKEPEKRHMSARDAASLAKEAQVKHMVISHFHPRADLRKLLRE
SKQIHPNTSLAVEGTSYLV

Specific function: Zinc Phosphodiesterase, Which Displays Some Trna 3'- Processing Endonuclease Activity. Probably Involved In Trna Maturation, By Removing A 3'Trailer From Precursor Trna. [C]

COG id: COG1234

COG function: function code R; Metal-dependent hydrolases of the beta-lactamase superfamily III

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: 3.1.26.11 [C]

Molecular weight: Translated: 29335; Mature: 29335

Theoretical pI: Translated: 5.36; Mature: 5.36

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMTKAFMLRVLGRYAPFPAKNSNCSGYLLTAGEDNFLFDCGNGVLSNLQNYLDLSQLSA
CCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHH
LFITHHHSDHVADLIALRHAYEDLQSRGEVTSKLPVYTPPYPQDTVAELEEYPGFDIKFL
HHEEECCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHCCCCCEEEE
AEGEDIIIEDKKISWSVTNHFLYCAAYRVEYKEKAFVYTGDNAYDNETIEDNSTADIINL
ECCCEEEEECCEEEEEHHHHEEEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCEEEC
SKGADLLLTEASLEEEWKKEPEKRHMSARDAASLAKEAQVKHMVISHFHPRADLRKLLRE
CCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SKQIHPNTSLAVEGTSYLV
HHCCCCCCEEEECCCEECC
>Mature Secondary Structure
MKMTKAFMLRVLGRYAPFPAKNSNCSGYLLTAGEDNFLFDCGNGVLSNLQNYLDLSQLSA
CCHHHHHHHHHHHHCCCCCCCCCCCCEEEEECCCCCEEEECCCHHHHHHHHHHHHHHHHH
LFITHHHSDHVADLIALRHAYEDLQSRGEVTSKLPVYTPPYPQDTVAELEEYPGFDIKFL
HHEEECCCHHHHHHHHHHHHHHHHHHCCCCHHCCCCCCCCCCCHHHHHHHHCCCCCEEEE
AEGEDIIIEDKKISWSVTNHFLYCAAYRVEYKEKAFVYTGDNAYDNETIEDNSTADIINL
ECCCEEEEECCEEEEEHHHHEEEEEEEEEEECCEEEEEECCCCCCCCCCCCCCCCCEEEC
SKGADLLLTEASLEEEWKKEPEKRHMSARDAASLAKEAQVKHMVISHFHPRADLRKLLRE
CCCCCEEEEHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHH
SKQIHPNTSLAVEGTSYLV
HHCCCCCCEEEECCCEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9579061; 9384377 [H]