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The map label for this gene is tnsB [H]

Identifier: 188584888

GI number: 188584888

Start: 256804

End: 258957

Strand: Direct

Name: tnsB [H]

Synonym: Nther_0246

Alternate gene names: 188584888

Gene position: 256804-258957 (Clockwise)

Preceding gene: 188584887

Following gene: 188584889

Centisome position: 8.11

GC content: 34.35

Gene sequence:

>2154_bases
ATGATATTTGTTAATGCGGTTTATCAGTTTGTAGATAAGAATGAAAGAATTCGCATTATTTATGTATCTACAGAAGAGGA
ATTATGTGCATATGTTTATATTGAAAAGGAGTTATCAGTACCAATTATTGAAGAGGTATCAGTAGTTAAAGAAGAGATTG
ATTCAGATAACTTGATAGAGATAACTGACCCGTACTCTGTTTTGATTGAAGAGGATAATCTAACTGAAACCCAAATAAAT
AAAAGAGAAGAAAGATGGGCTATTATTGAACAATACTGGGAGCCCCAGAAAGAAAAATTATTAAATAAGTCCACACGTAT
GAAAAAGTTTAAGGAAATTTCGCAGTTACATAATATTTCGTTGATGAGAGTCAGGCGTATGTTTAGCCAATTTTGGCAGA
GAGGGATGACTCCAAATGCATGTTTACCCGATTATAAAAATTCTGGCGCACCAGGGAGAGAAAAGAACTTTATAAAAAAG
ACTGGTAGGCCGAGAAATATAACAAATAAGGAATTTTCTTTAGGGATAAATGTGGATGATAAAGTCAAAAAACAGTTTGA
GGTTTCAATTAATAAATACTATAGGACAAAACAAAAGAAATCTTTGCGTGAAGTGTATTCACAAATGCTAGAGGATTTTT
ATTCGGTAACAATTAAAGAACAAGGAGAAACTAAAAGAATTCTGCGGGATCCGTCAAATGTCCCTACATACGAGCAGTTT
TATTACTGGTTTAAAAAGGCTGAAAATCCTGGTGTTGATTTTGTAAAGCGAGAGGGACAAAAGGATTACGAACTTACTAA
AAGACCAATAACAGGAACCACGAAACAGGAGGCACCAGGCCCGGGTTTTAGATTTCAAATTGATGCAACACCTTCAGATA
TATATATTGTTAGTGAGACAGATAGGAGTAAAGTTGTAGGGAGAGCAACTATTTATGCTATAGTTGATGTATTCAGCAGA
ATGATTACTGGTATATATGTCGGTTTAGAAACAAACTCCTGGAATGGTGCAATGATGTCATTGGACTCAATGGTAGCCAA
TAAATCTGAATTGTGTGCTGATTATGGAGTGCCACTGGATGAAGAACTATGGCCTAGCTCTCATCTACCTGAAGCCATTA
TTGCTGATAGAGGAGAAATGTTAGGGCATGGAGTTGAAAACCTTATAAACAATTTTAAAGTAGCAGTTGAAAATACTTCT
CCATACAGGGCTGATTTAAAGCCTATTGTAGAGCGTTTGTTTAGGACTATTAATGAAAAAATTAAAGGATTTCTTCCTGG
GGCAGTCATGAAGGACACTAGAAAAAGAGGAGACCCTGACTACCGATTAGATGCAAAGTTAACTTTAAAAGAAATTAGGA
GAATAATTTTATTGTCGGTAATAGATCATAATTGGAGTCCTATTGAACACTATCAACTTTCTCCTGAAATGGTAAAAGAT
AATGTCCGACCTATTCCCTTAGAACTGTGGAATTGGGGAATAGAAAACCAGAAAGGTTGTTTAAGGAAGGTTGATCGATT
AGATTTTCGCCTTGGCATTTTACCACGGGCTAAAGCTACTTTTTCAAGAGGGACAGTAACCTTTCATAAACTGAAATATG
GCGCTTCAGAGCTACTAGAGAACTATAATCATATGAAGCTAACAGATACAAAGGTGGATATAGTTTATGACCCAAGAAAA
ATTGATAATATATACATGATTAGAAATGATTCAAAACCTATTTTATTGAACTTGCTTGATCAAAGTAGAGGCTTTGCTGG
CCTATCTTTAGAAGAAGTTATTGAAATTAACAAAAAAGCTTCAACACTAAAAAAATCTGCTGATGATGAACGATTAAAAA
ATAGGATCACGCTGAATCAAGAGATTAATACAATTGCTAAAAAAGCAACTAAAAAAACAAATGAGGAATCAAATATTTCA
ACTTCAAAATCGAAGAGGTTAAAAGACATTAATAAAAATAGATCTGAAGAAAAGGCCATTCAAAAGAAGGAAGAGACATT
GATACAAAGAGAAGAAAACGAGGACCCAGCAGAAATTGTTTCTATAGAAGAGGAATTAAAAGAACCAAATAATGGCAGTG
AAGGGTCAAAATCAGGTAATAGTGAAGTTTATGACTTAATAAGGAGGCGTAGGGATGAGCGTAGAAAAAAATAG

Upstream 100 bases:

>100_bases
AATATATAAAAGTAGATCTTTTTGAACCGTTAAATTTGGACAGGCCTCATCTAAGAGTTGACTTAACTAATAAGAGCAAG
GTTTTGGGGGAGGACGTATC

Downstream 100 bases:

>100_bases
GACAGTTGATGTTTTTGAGTATAAAACAGCACATTATACGCCTCAGAAGATAAAAGATTATGCGGGGAACCCACTAATTG
AAGCACTACCACCTATTCTT

Product: Integrase catalytic region

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 717; Mature: 717

Protein sequence:

>717_residues
MIFVNAVYQFVDKNERIRIIYVSTEEELCAYVYIEKELSVPIIEEVSVVKEEIDSDNLIEITDPYSVLIEEDNLTETQIN
KREERWAIIEQYWEPQKEKLLNKSTRMKKFKEISQLHNISLMRVRRMFSQFWQRGMTPNACLPDYKNSGAPGREKNFIKK
TGRPRNITNKEFSLGINVDDKVKKQFEVSINKYYRTKQKKSLREVYSQMLEDFYSVTIKEQGETKRILRDPSNVPTYEQF
YYWFKKAENPGVDFVKREGQKDYELTKRPITGTTKQEAPGPGFRFQIDATPSDIYIVSETDRSKVVGRATIYAIVDVFSR
MITGIYVGLETNSWNGAMMSLDSMVANKSELCADYGVPLDEELWPSSHLPEAIIADRGEMLGHGVENLINNFKVAVENTS
PYRADLKPIVERLFRTINEKIKGFLPGAVMKDTRKRGDPDYRLDAKLTLKEIRRIILLSVIDHNWSPIEHYQLSPEMVKD
NVRPIPLELWNWGIENQKGCLRKVDRLDFRLGILPRAKATFSRGTVTFHKLKYGASELLENYNHMKLTDTKVDIVYDPRK
IDNIYMIRNDSKPILLNLLDQSRGFAGLSLEEVIEINKKASTLKKSADDERLKNRITLNQEINTIAKKATKKTNEESNIS
TSKSKRLKDINKNRSEEKAIQKKEETLIQREENEDPAEIVSIEEELKEPNNGSEGSKSGNSEVYDLIRRRRDERRKK

Sequences:

>Translated_717_residues
MIFVNAVYQFVDKNERIRIIYVSTEEELCAYVYIEKELSVPIIEEVSVVKEEIDSDNLIEITDPYSVLIEEDNLTETQIN
KREERWAIIEQYWEPQKEKLLNKSTRMKKFKEISQLHNISLMRVRRMFSQFWQRGMTPNACLPDYKNSGAPGREKNFIKK
TGRPRNITNKEFSLGINVDDKVKKQFEVSINKYYRTKQKKSLREVYSQMLEDFYSVTIKEQGETKRILRDPSNVPTYEQF
YYWFKKAENPGVDFVKREGQKDYELTKRPITGTTKQEAPGPGFRFQIDATPSDIYIVSETDRSKVVGRATIYAIVDVFSR
MITGIYVGLETNSWNGAMMSLDSMVANKSELCADYGVPLDEELWPSSHLPEAIIADRGEMLGHGVENLINNFKVAVENTS
PYRADLKPIVERLFRTINEKIKGFLPGAVMKDTRKRGDPDYRLDAKLTLKEIRRIILLSVIDHNWSPIEHYQLSPEMVKD
NVRPIPLELWNWGIENQKGCLRKVDRLDFRLGILPRAKATFSRGTVTFHKLKYGASELLENYNHMKLTDTKVDIVYDPRK
IDNIYMIRNDSKPILLNLLDQSRGFAGLSLEEVIEINKKASTLKKSADDERLKNRITLNQEINTIAKKATKKTNEESNIS
TSKSKRLKDINKNRSEEKAIQKKEETLIQREENEDPAEIVSIEEELKEPNNGSEGSKSGNSEVYDLIRRRRDERRKK
>Mature_717_residues
MIFVNAVYQFVDKNERIRIIYVSTEEELCAYVYIEKELSVPIIEEVSVVKEEIDSDNLIEITDPYSVLIEEDNLTETQIN
KREERWAIIEQYWEPQKEKLLNKSTRMKKFKEISQLHNISLMRVRRMFSQFWQRGMTPNACLPDYKNSGAPGREKNFIKK
TGRPRNITNKEFSLGINVDDKVKKQFEVSINKYYRTKQKKSLREVYSQMLEDFYSVTIKEQGETKRILRDPSNVPTYEQF
YYWFKKAENPGVDFVKREGQKDYELTKRPITGTTKQEAPGPGFRFQIDATPSDIYIVSETDRSKVVGRATIYAIVDVFSR
MITGIYVGLETNSWNGAMMSLDSMVANKSELCADYGVPLDEELWPSSHLPEAIIADRGEMLGHGVENLINNFKVAVENTS
PYRADLKPIVERLFRTINEKIKGFLPGAVMKDTRKRGDPDYRLDAKLTLKEIRRIILLSVIDHNWSPIEHYQLSPEMVKD
NVRPIPLELWNWGIENQKGCLRKVDRLDFRLGILPRAKATFSRGTVTFHKLKYGASELLENYNHMKLTDTKVDIVYDPRK
IDNIYMIRNDSKPILLNLLDQSRGFAGLSLEEVIEINKKASTLKKSADDERLKNRITLNQEINTIAKKATKKTNEESNIS
TSKSKRLKDINKNRSEEKAIQKKEETLIQREENEDPAEIVSIEEELKEPNNGSEGSKSGNSEVYDLIRRRRDERRKK

Specific function: Sequence-specific, DNA-binding protein required for Tn7 transposition. Recognizes sequences necessary for recombination at both left and right ends of Tn7 and, together with tnsA, forms the transposase. TnsB executes the 5'-DNA strand breakage and joining

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 integrase catalytic domain [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001584
- InterPro:   IPR012337 [H]

Pfam domain/function: PF00665 rve [H]

EC number: NA

Molecular weight: Translated: 83381; Mature: 83381

Theoretical pI: Translated: 9.12; Mature: 9.12

Prosite motif: PS50994 INTEGRASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIFVNAVYQFVDKNERIRIIYVSTEEELCAYVYIEKELSVPIIEEVSVVKEEIDSDNLIE
CEEHHHHHHHHCCCCCEEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEE
ITDPYSVLIEEDNLTETQINKREERWAIIEQYWEPQKEKLLNKSTRMKKFKEISQLHNIS
EECCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LMRVRRMFSQFWQRGMTPNACLPDYKNSGAPGREKNFIKKTGRPRNITNKEFSLGINVDD
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEECCCCH
KVKKQFEVSINKYYRTKQKKSLREVYSQMLEDFYSVTIKEQGETKRILRDPSNVPTYEQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHCCCCCCCCHHHH
YYWFKKAENPGVDFVKREGQKDYELTKRPITGTTKQEAPGPGFRFQIDATPSDIYIVSET
HHHHHHCCCCCCHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECC
DRSKVVGRATIYAIVDVFSRMITGIYVGLETNSWNGAMMSLDSMVANKSELCADYGVPLD
CCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCHHHHHHHCCCCCC
EELWPSSHLPEAIIADRGEMLGHGVENLINNFKVAVENTSPYRADLKPIVERLFRTINEK
HHCCCCCCCCHHHHHCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
IKGFLPGAVMKDTRKRGDPDYRLDAKLTLKEIRRIILLSVIDHNWSPIEHYQLSPEMVKD
HHHCCCCHHHHHHHHCCCCCEEECCEEHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHC
NVRPIPLELWNWGIENQKGCLRKVDRLDFRLGILPRAKATFSRGTVTFHKLKYGASELLE
CCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCEEEEHHHCCHHHHHH
NYNHMKLTDTKVDIVYDPRKIDNIYMIRNDSKPILLNLLDQSRGFAGLSLEEVIEINKKA
CCCCEEEECCEEEEEECCCCCCEEEEEECCCCCEEEEEHHHCCCCCCCCHHHHHHHHHHH
STLKKSADDERLKNRITLNQEINTIAKKATKKTNEESNISTSKSKRLKDINKNRSEEKAI
HHHHHCCCHHHHHHHEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHH
QKKEETLIQREENEDPAEIVSIEEELKEPNNGSEGSKSGNSEVYDLIRRRRDERRKK
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MIFVNAVYQFVDKNERIRIIYVSTEEELCAYVYIEKELSVPIIEEVSVVKEEIDSDNLIE
CEEHHHHHHHHCCCCCEEEEEEECCCCEEEEEEEECCCCCCHHHHHHHHHHHCCCCCEEE
ITDPYSVLIEEDNLTETQINKREERWAIIEQYWEPQKEKLLNKSTRMKKFKEISQLHNIS
EECCEEEEEECCCCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LMRVRRMFSQFWQRGMTPNACLPDYKNSGAPGREKNFIKKTGRPRNITNKEFSLGINVDD
HHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHCCCCCCCCCCCEEEECCCCH
KVKKQFEVSINKYYRTKQKKSLREVYSQMLEDFYSVTIKEQGETKRILRDPSNVPTYEQF
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEEEEECCCCHHHHHCCCCCCCCHHHH
YYWFKKAENPGVDFVKREGQKDYELTKRPITGTTKQEAPGPGFRFQIDATPSDIYIVSET
HHHHHHCCCCCCHHHHHCCCCCHHHHCCCCCCCCCCCCCCCCEEEEEECCCCCEEEEECC
DRSKVVGRATIYAIVDVFSRMITGIYVGLETNSWNGAMMSLDSMVANKSELCADYGVPLD
CCHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCHHHHHHHHHCCHHHHHHHCCCCCC
EELWPSSHLPEAIIADRGEMLGHGVENLINNFKVAVENTSPYRADLKPIVERLFRTINEK
HHCCCCCCCCHHHHHCCHHHHHHHHHHHHCCEEEEEECCCCCHHHHHHHHHHHHHHHHHH
IKGFLPGAVMKDTRKRGDPDYRLDAKLTLKEIRRIILLSVIDHNWSPIEHYQLSPEMVKD
HHHCCCCHHHHHHHHCCCCCEEECCEEHHHHHHHHHHHHHHCCCCCCCHHCCCCHHHHHC
NVRPIPLELWNWGIENQKGCLRKVDRLDFRLGILPRAKATFSRGTVTFHKLKYGASELLE
CCCCCCHHHHCCCCCCCCHHHHHHHHHHHHCCCCCCCCHHHCCCCEEEEHHHCCHHHHHH
NYNHMKLTDTKVDIVYDPRKIDNIYMIRNDSKPILLNLLDQSRGFAGLSLEEVIEINKKA
CCCCEEEECCEEEEEECCCCCCEEEEEECCCCCEEEEEHHHCCCCCCCCHHHHHHHHHHH
STLKKSADDERLKNRITLNQEINTIAKKATKKTNEESNISTSKSKRLKDINKNRSEEKAI
HHHHHCCCHHHHHHHEECCHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHCCCHHHHHH
QKKEETLIQREENEDPAEIVSIEEELKEPNNGSEGSKSGNSEVYDLIRRRRDERRKK
HHHHHHHHHHCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2156235; 1655576; 1657979; 8947057; 10704304 [H]