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The map label for this gene is rocF [H]

Identifier: 188584878

GI number: 188584878

Start: 245424

End: 246317

Strand: Direct

Name: rocF [H]

Synonym: Nther_0236

Alternate gene names: 188584878

Gene position: 245424-246317 (Clockwise)

Preceding gene: 188584877

Following gene: 188584879

Centisome position: 7.75

GC content: 39.71

Gene sequence:

>894_bases
ATGAATACATCAATAATTGGAGTTCCTATGGATTTAGGAGCCAACCGTAGAGGAGTAGATATGGGTCCAAGTGCAATCAG
GTATGCTGATTTAAGAGCAAAACTAGAGGAAATTGGAATTGATGCCAAAGATAAAGGTAATATTTCTGTTCCTCTGCCTG
AAATAGACAGCATGGAAGCAGAGGAAATTAAGTATCCCAAACAAATTGAAGCTGTTACAAATTCCCTAGCACTGCAAGTT
TCAAAAGAAGCAAGCCAAAATAGAATTCCTATTATACTTGGCGGTGATCATAGTATAGCAATTGGTAGTATTGCAGGTAT
GAAACAAGCCGTTGGAGAGCTGGGTGTATTATGGTTTGATGCTCATGCTGATTTAAATACATTTGAAACAAGTCCTTCAG
GGAATATTCATGGCATGCCCTTAGCCTTTTCTTTAGGTTATGGAAACAGCAACTTGGTTAACTGTCTGGAAAAAGGTCAG
AAAATAAAACCTGAAAATACAGTATTAATTGGGACTAGATCTATAGATGAACCGGAAAAAGAACTAATTGAAAAATCTGG
TGTGACTGTATTTACCATGTACGATTTGGATAAAATGGGAATGAACCGTGTAATGGAGGAAGCATTGTCAATTGTCACTT
CCAATACTGATGGAGTTCACGTGAGTTTAGACATGGATGCACTTGACCCGGTAGAAGCGCCAGGAGTAGGTACCCCTGTC
CGAGGTGGAATTAATTACCGGGAGGTGCATTTAGCCATGGAGCTGATAGCAGAGGCACAATGTATAAACTCCATGGAAGT
AGTAGAAGTTAATCCTATTTTAGACAGTGTCAACCGGACCGGTGATTTGGCTGTAGAGTTAGTAGCTTCGGCTTTAGGTG
AACGTGTATTATAA

Upstream 100 bases:

>100_bases
AATTTTCTTCCTAGTCCTTAAAATGGATGGTAGTGAAATCTTGAGAATGAGTGAGTATATTGAAGAAATAATTAATTTAT
AGAGGGAGGATGATCTACCA

Downstream 100 bases:

>100_bases
AGTTGTGTTTATTAACTAATTTTGTAGGAGGGAATTATATGTTAAAGTGGGCAATTTTTTCTCCTCACCCTCCCCTATTA
ATTCCTGAAATAGGTAAACA

Product: arginase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 297; Mature: 297

Protein sequence:

>297_residues
MNTSIIGVPMDLGANRRGVDMGPSAIRYADLRAKLEEIGIDAKDKGNISVPLPEIDSMEAEEIKYPKQIEAVTNSLALQV
SKEASQNRIPIILGGDHSIAIGSIAGMKQAVGELGVLWFDAHADLNTFETSPSGNIHGMPLAFSLGYGNSNLVNCLEKGQ
KIKPENTVLIGTRSIDEPEKELIEKSGVTVFTMYDLDKMGMNRVMEEALSIVTSNTDGVHVSLDMDALDPVEAPGVGTPV
RGGINYREVHLAMELIAEAQCINSMEVVEVNPILDSVNRTGDLAVELVASALGERVL

Sequences:

>Translated_297_residues
MNTSIIGVPMDLGANRRGVDMGPSAIRYADLRAKLEEIGIDAKDKGNISVPLPEIDSMEAEEIKYPKQIEAVTNSLALQV
SKEASQNRIPIILGGDHSIAIGSIAGMKQAVGELGVLWFDAHADLNTFETSPSGNIHGMPLAFSLGYGNSNLVNCLEKGQ
KIKPENTVLIGTRSIDEPEKELIEKSGVTVFTMYDLDKMGMNRVMEEALSIVTSNTDGVHVSLDMDALDPVEAPGVGTPV
RGGINYREVHLAMELIAEAQCINSMEVVEVNPILDSVNRTGDLAVELVASALGERVL
>Mature_297_residues
MNTSIIGVPMDLGANRRGVDMGPSAIRYADLRAKLEEIGIDAKDKGNISVPLPEIDSMEAEEIKYPKQIEAVTNSLALQV
SKEASQNRIPIILGGDHSIAIGSIAGMKQAVGELGVLWFDAHADLNTFETSPSGNIHGMPLAFSLGYGNSNLVNCLEKGQ
KIKPENTVLIGTRSIDEPEKELIEKSGVTVFTMYDLDKMGMNRVMEEALSIVTSNTDGVHVSLDMDALDPVEAPGVGTPV
RGGINYREVHLAMELIAEAQCINSMEVVEVNPILDSVNRTGDLAVELVASALGERVL

Specific function: Catalyzes The Formation Of Putrescine From Agmatine. [C]

COG id: COG0010

COG function: function code E; Arginase/agmatinase/formimionoglutamate hydrolase, arginase family

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the arginase family [H]

Homologues:

Organism=Homo sapiens, GI4502215, Length=303, Percent_Identity=37.2937293729373, Blast_Score=197, Evalue=1e-50,
Organism=Homo sapiens, GI10947139, Length=304, Percent_Identity=36.8421052631579, Blast_Score=187, Evalue=7e-48,
Organism=Homo sapiens, GI37537722, Length=306, Percent_Identity=27.7777777777778, Blast_Score=76, Evalue=3e-14,
Organism=Escherichia coli, GI1789306, Length=280, Percent_Identity=24.6428571428571, Blast_Score=71, Evalue=7e-14,
Organism=Caenorhabditis elegans, GI71996843, Length=203, Percent_Identity=41.871921182266, Blast_Score=157, Evalue=6e-39,
Organism=Saccharomyces cerevisiae, GI6325146, Length=322, Percent_Identity=33.8509316770186, Blast_Score=175, Evalue=9e-45,
Organism=Drosophila melanogaster, GI24638886, Length=309, Percent_Identity=34.3042071197411, Blast_Score=163, Evalue=1e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014033
- InterPro:   IPR006035
- InterPro:   IPR020855 [H]

Pfam domain/function: PF00491 Arginase [H]

EC number: =3.5.3.1 [H]

Molecular weight: Translated: 31838; Mature: 31838

Theoretical pI: Translated: 4.31; Mature: 4.31

Prosite motif: PS00147 ARGINASE_1 ; PS00148 ARGINASE_2 ; PS01053 ARGINASE_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.4 %Met     (Translated Protein)
5.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.4 %Met     (Mature Protein)
5.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTSIIGVPMDLGANRRGVDMGPSAIRYADLRAKLEEIGIDAKDKGNISVPLPEIDSMEA
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCH
EEIKYPKQIEAVTNSLALQVSKEASQNRIPIILGGDHSIAIGSIAGMKQAVGELGVLWFD
HHCCCCHHHHHHHHHHHEEEECCCCCCCCCEEECCCCCEEEHHHHHHHHHHHHHCEEEEE
AHADLNTFETSPSGNIHGMPLAFSLGYGNSNLVNCLEKGQKIKPENTVLIGTRSIDEPEK
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCEEEEEECCCCCHHH
ELIEKSGVTVFTMYDLDKMGMNRVMEEALSIVTSNTDGVHVSLDMDALDPVEAPGVGTPV
HHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCC
RGGINYREVHLAMELIAEAQCINSMEVVEVNPILDSVNRTGDLAVELVASALGERVL
CCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHCCCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure
MNTSIIGVPMDLGANRRGVDMGPSAIRYADLRAKLEEIGIDAKDKGNISVPLPEIDSMEA
CCCCEEEEECCCCCCCCCCCCCCHHHHHHHHHHHHHHHCCCCCCCCCEEECCCCCCCCCH
EEIKYPKQIEAVTNSLALQVSKEASQNRIPIILGGDHSIAIGSIAGMKQAVGELGVLWFD
HHCCCCHHHHHHHHHHHEEEECCCCCCCCCEEECCCCCEEEHHHHHHHHHHHHHCEEEEE
AHADLNTFETSPSGNIHGMPLAFSLGYGNSNLVNCLEKGQKIKPENTVLIGTRSIDEPEK
CCCCCCCCCCCCCCCCCCCEEEEEECCCCCHHHHHHHCCCCCCCCCEEEEEECCCCCHHH
ELIEKSGVTVFTMYDLDKMGMNRVMEEALSIVTSNTDGVHVSLDMDALDPVEAPGVGTPV
HHHHHCCCEEEEEECCHHHHHHHHHHHHHHHHCCCCCCEEEEEECCCCCCCCCCCCCCCC
RGGINYREVHLAMELIAEAQCINSMEVVEVNPILDSVNRTGDLAVELVASALGERVL
CCCCCHHHHHHHHHHHHHHHHCCCEEEEEECHHHHHCCCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9339546 [H]