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The map label for this gene is cysS [H]

Identifier: 188584813

GI number: 188584813

Start: 195331

End: 196740

Strand: Direct

Name: cysS [H]

Synonym: Nther_0171

Alternate gene names: 188584813

Gene position: 195331-196740 (Clockwise)

Preceding gene: 188584812

Following gene: 188584814

Centisome position: 6.17

GC content: 38.3

Gene sequence:

>1410_bases
ATGAAAATGAGCATAAAGCTCTTTAATACAATGAAAAAAAAGAAAGAGGAATTTGTCCCCAAACAAGAAGGAAAAGTTGA
TATGTATGTTTGTGGTCCAACAGTATATGATTATTTTCATGTCGGAAATGCCAGGGCATTTTTAATTTTCGATGTGGTAC
GCAGATATTTAGATTACAAAGGTTATCAAGTGACTTATGTTCAAAACATTACTGATATAGAAGACAAAATGATTAATAGA
GCTAATGAAGACGGGATAACTGTTGCTGAGTTAGCAGAGAAATACACTAGTGAGTACTTTGATGATGCTCGTGCTATAGG
TATTAAAGATGCAGACCATCATCCGAAAGCTACTGAGCATATAGCCGAAATTATTGAAATTATCCAGGATCTTCTGGATA
AAGGTTATGCCTACCGTCAAGGAGGAGACGTTTACTTTGATACAGCAGCCTTTGACGATTACGGAAAATTATGCAGACAA
GACCCTGAAGAGCTGGTGTCTGGTGCTAGAGTGGCAGTGGATGATCGTAAGAAAAATCCTGCAGACTTTGTTTTATGGAA
AAGCAAAAAGGAAGGGGAACCGGCCTGGGACAGCCCCTTTGGTCAAGGTCGGCCAGGTTGGCATATAGAGTGTTCTGCTA
TGAGCATGAAATACTTGGATACGCCCCTGGATATCCATGCTGGGGGATCCGACTTAGTTTTCCCACACCACGAAAATGAA
GTGGCTCAGAGTGAGGCCGCTACAGGACAAGACTTTTGCAAGTATTGGATGCATGTAGGTTATTTAGAGATTGATAACCG
AAAAATGTCGAAATCTATGGGGAACTTTTTAACTGTTCGTGACTTTAGACAAAAGTATGATCCTCGTGTTTTAAGATTGT
TTTTAATGAGTGCTCACTATAGAAGTCCTCTTAATTTTACCGATGAATTAATTGAACAGGCAAAAAGCAGCTTAGACAGA
TTGGATAACTTTTACAATAATCTTAAGTTTTATCTTGAAAAAAGTCGAGAACAAGAACTTACGGATAAAGAGCAAGATAT
TATGGAATATCTAAAACAAGCTAGAACAGAATTTGAAGCAGCTATGGATGATGATTTCAATACGGCCAGTGCACTGGCTG
TTATATTTTCATTAGCAAAAGAAATTAACAGCTATATTGGAAATGGAGGAGAGAATGCCCTAGTTTTCAAAAACACTTAT
GACCTGTTGTTAGAATTTGATCAAGTTATGGGGCTCCTGCCAGAAACAGATGAAATATTACCGGAAGAAATAGAGCAAAA
AATTCAAGAACGAGAGCAAGCTCGCAAACAAAAAGATTTTCAACGAGCAGACGCAATTAGAGATGAACTTCGTGACCGAG
GAATTATTTTAGAGGATACTCCTGAAGGAGTGAGGTGGAAACGAACTTGA

Upstream 100 bases:

>100_bases
CATCACGAGCTTCCAGACCCTGTTGCTAGACAAATTGAATATTTGGAAAGAAAAATTGAAAAGTTGCGTTTTGAAATAGA
AGATATCAAGAGAGGTGATC

Downstream 100 bases:

>100_bases
AAGCATTTGAAAAAACTATAGAACAAAAGACAAAAACGGGTCAATTTTCACCTCAAAGCTTAGCTTATTTAGGGGATGCT
GTTTATGAGTTATATGTCAG

Product: cysteinyl-tRNA synthetase

Products: NA

Alternate protein names: Cysteine--tRNA ligase; CysRS [H]

Number of amino acids: Translated: 469; Mature: 469

Protein sequence:

>469_residues
MKMSIKLFNTMKKKKEEFVPKQEGKVDMYVCGPTVYDYFHVGNARAFLIFDVVRRYLDYKGYQVTYVQNITDIEDKMINR
ANEDGITVAELAEKYTSEYFDDARAIGIKDADHHPKATEHIAEIIEIIQDLLDKGYAYRQGGDVYFDTAAFDDYGKLCRQ
DPEELVSGARVAVDDRKKNPADFVLWKSKKEGEPAWDSPFGQGRPGWHIECSAMSMKYLDTPLDIHAGGSDLVFPHHENE
VAQSEAATGQDFCKYWMHVGYLEIDNRKMSKSMGNFLTVRDFRQKYDPRVLRLFLMSAHYRSPLNFTDELIEQAKSSLDR
LDNFYNNLKFYLEKSREQELTDKEQDIMEYLKQARTEFEAAMDDDFNTASALAVIFSLAKEINSYIGNGGENALVFKNTY
DLLLEFDQVMGLLPETDEILPEEIEQKIQEREQARKQKDFQRADAIRDELRDRGIILEDTPEGVRWKRT

Sequences:

>Translated_469_residues
MKMSIKLFNTMKKKKEEFVPKQEGKVDMYVCGPTVYDYFHVGNARAFLIFDVVRRYLDYKGYQVTYVQNITDIEDKMINR
ANEDGITVAELAEKYTSEYFDDARAIGIKDADHHPKATEHIAEIIEIIQDLLDKGYAYRQGGDVYFDTAAFDDYGKLCRQ
DPEELVSGARVAVDDRKKNPADFVLWKSKKEGEPAWDSPFGQGRPGWHIECSAMSMKYLDTPLDIHAGGSDLVFPHHENE
VAQSEAATGQDFCKYWMHVGYLEIDNRKMSKSMGNFLTVRDFRQKYDPRVLRLFLMSAHYRSPLNFTDELIEQAKSSLDR
LDNFYNNLKFYLEKSREQELTDKEQDIMEYLKQARTEFEAAMDDDFNTASALAVIFSLAKEINSYIGNGGENALVFKNTY
DLLLEFDQVMGLLPETDEILPEEIEQKIQEREQARKQKDFQRADAIRDELRDRGIILEDTPEGVRWKRT
>Mature_469_residues
MKMSIKLFNTMKKKKEEFVPKQEGKVDMYVCGPTVYDYFHVGNARAFLIFDVVRRYLDYKGYQVTYVQNITDIEDKMINR
ANEDGITVAELAEKYTSEYFDDARAIGIKDADHHPKATEHIAEIIEIIQDLLDKGYAYRQGGDVYFDTAAFDDYGKLCRQ
DPEELVSGARVAVDDRKKNPADFVLWKSKKEGEPAWDSPFGQGRPGWHIECSAMSMKYLDTPLDIHAGGSDLVFPHHENE
VAQSEAATGQDFCKYWMHVGYLEIDNRKMSKSMGNFLTVRDFRQKYDPRVLRLFLMSAHYRSPLNFTDELIEQAKSSLDR
LDNFYNNLKFYLEKSREQELTDKEQDIMEYLKQARTEFEAAMDDDFNTASALAVIFSLAKEINSYIGNGGENALVFKNTY
DLLLEFDQVMGLLPETDEILPEEIEQKIQEREQARKQKDFQRADAIRDELRDRGIILEDTPEGVRWKRT

Specific function: Unknown

COG id: COG0215

COG function: function code J; Cysteinyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI13375695, Length=421, Percent_Identity=34.916864608076, Blast_Score=260, Evalue=2e-69,
Organism=Homo sapiens, GI10835051, Length=337, Percent_Identity=33.2344213649852, Blast_Score=202, Evalue=4e-52,
Organism=Homo sapiens, GI303304987, Length=438, Percent_Identity=30.5936073059361, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI21269877, Length=438, Percent_Identity=30.5936073059361, Blast_Score=202, Evalue=5e-52,
Organism=Homo sapiens, GI62240992, Length=337, Percent_Identity=33.2344213649852, Blast_Score=202, Evalue=6e-52,
Organism=Escherichia coli, GI1786737, Length=476, Percent_Identity=47.2689075630252, Blast_Score=414, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI17509795, Length=361, Percent_Identity=33.7950138504155, Blast_Score=204, Evalue=1e-52,
Organism=Caenorhabditis elegans, GI17509797, Length=361, Percent_Identity=33.7950138504155, Blast_Score=203, Evalue=1e-52,
Organism=Saccharomyces cerevisiae, GI6324082, Length=320, Percent_Identity=35, Blast_Score=215, Evalue=1e-56,
Organism=Drosophila melanogaster, GI19922218, Length=389, Percent_Identity=36.7609254498715, Blast_Score=253, Evalue=2e-67,
Organism=Drosophila melanogaster, GI19922364, Length=369, Percent_Identity=34.6883468834688, Blast_Score=204, Evalue=1e-52,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR015803
- InterPro:   IPR015273
- InterPro:   IPR014729
- InterPro:   IPR009080 [H]

Pfam domain/function: PF09190 DALR_2; PF01406 tRNA-synt_1e [H]

EC number: =6.1.1.16 [H]

Molecular weight: Translated: 54417; Mature: 54417

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKMSIKLFNTMKKKKEEFVPKQEGKVDMYVCGPTVYDYFHVGNARAFLIFDVVRRYLDYK
CCEEHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHCCCCCHHHHHHHHHHHHHCCC
GYQVTYVQNITDIEDKMINRANEDGITVAELAEKYTSEYFDDARAIGIKDADHHPKATEH
CCEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHH
IAEIIEIIQDLLDKGYAYRQGGDVYFDTAAFDDYGKLCRQDPEELVSGARVAVDDRKKNP
HHHHHHHHHHHHHCCCEEECCCCEEEECCCHHHHHHHHCCCHHHHHCCCEEEECCCCCCC
ADFVLWKSKKEGEPAWDSPFGQGRPGWHIECSAMSMKYLDTPLDIHAGGSDLVFPHHENE
CCEEEECCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCCCEEECCCCCCCCCCCCCH
VAQSEAATGQDFCKYWMHVGYLEIDNRKMSKSMGNFLTVRDFRQKYDPRVLRLFLMSAHY
HHHHHCCCHHHHHHHHHHCCEEEECCHHHHHHHCCEEHHHHHHHHHCHHHHHHHHHHHHC
RSPLNFTDELIEQAKSSLDRLDNFYNNLKFYLEKSREQELTDKEQDIMEYLKQARTEFEA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AMDDDFNTASALAVIFSLAKEINSYIGNGGENALVFKNTYDLLLEFDQVMGLLPETDEIL
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCCCCCHHCC
PEEIEQKIQEREQARKQKDFQRADAIRDELRDRGIILEDTPEGVRWKRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCC
>Mature Secondary Structure
MKMSIKLFNTMKKKKEEFVPKQEGKVDMYVCGPTVYDYFHVGNARAFLIFDVVRRYLDYK
CCEEHHHHHHHHHHHHHCCCCCCCCEEEEEECCHHHHHHCCCCCHHHHHHHHHHHHHCCC
GYQVTYVQNITDIEDKMINRANEDGITVAELAEKYTSEYFDDARAIGIKDADHHPKATEH
CCEEEEECCCHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHEECCCCCCCCCHHHHH
IAEIIEIIQDLLDKGYAYRQGGDVYFDTAAFDDYGKLCRQDPEELVSGARVAVDDRKKNP
HHHHHHHHHHHHHCCCEEECCCCEEEECCCHHHHHHHHCCCHHHHHCCCEEEECCCCCCC
ADFVLWKSKKEGEPAWDSPFGQGRPGWHIECSAMSMKYLDTPLDIHAGGSDLVFPHHENE
CCEEEECCCCCCCCCCCCCCCCCCCCCEEEEHHHHHHHCCCCCEEECCCCCCCCCCCCCH
VAQSEAATGQDFCKYWMHVGYLEIDNRKMSKSMGNFLTVRDFRQKYDPRVLRLFLMSAHY
HHHHHCCCHHHHHHHHHHCCEEEECCHHHHHHHCCEEHHHHHHHHHCHHHHHHHHHHHHC
RSPLNFTDELIEQAKSSLDRLDNFYNNLKFYLEKSREQELTDKEQDIMEYLKQARTEFEA
CCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHH
AMDDDFNTASALAVIFSLAKEINSYIGNGGENALVFKNTYDLLLEFDQVMGLLPETDEIL
HHCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCEEEEECHHHHHHHHHHHHCCCCCCHHCC
PEEIEQKIQEREQARKQKDFQRADAIRDELRDRGIILEDTPEGVRWKRT
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEECCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11997336 [H]