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The map label for this gene is mltD [H]

Identifier: 188528364

GI number: 188528364

Start: 1595196

End: 1596308

Strand: Reverse

Name: mltD [H]

Synonym: HPSH_08190

Alternate gene names: 188528364

Gene position: 1596308-1595196 (Counterclockwise)

Preceding gene: 188528365

Following gene: 188528363

Centisome position: 99.24

GC content: 39.17

Gene sequence:

>1113_bases
ATGAAGTGTTTTAGTCAAAAATGGTTGGTTTTTTTTGTTACCCGTTGGCTTTTATTGGCTTCTTTAGGCCATGCGAAAAT
GGCTTTTGAATCCAATATTGACACCAAAGCGTTAGAGGCTTTTGGGGTTAATGCGGGCTTTTTATCCCAAATGCCAAACG
CTTTAAAAAAAATGAATAAAGAAGAAGAATGGAAAAAGTTAGTCAAAAGATTTGATGTGAATTACCAGTTCATCCCCATC
ATTAAAAACATGCTAATAAAAGCGAGCGTGCCGCAAGAATTTTTATTTTTAGCCATGGCAGAGTCTAAATTTTCATCAAG
GGCTTATAGCAGGAAAAAAGCGGTAGGGATTTGGCAATTCATGCCAAGCACGGCTAAAGAATTAGGGCTTAAAGTCAATC
ATTACATTGATGAGAGAAGAGATCCCATTAAAAGCACTCAAGCAGCGATCACTTACTTGAAACGGCTCTACAAGCAAACC
GGGGAGTGGTATTTGGTCGCTATGGCGTATAATTACGGCTTGCGCAAGGTTCAAAACGCCATTAAAGCCGCCGGCACTTC
GGACATTAAGATTTTGTTAGATGAAGATAAAAAGTATCTCCCTAAAGAGACACGAGAGTATATCCGCTCCATTCTAAGCC
TGGCGTTAAAATTCAACAGCCTAGACAATCTCAAAGATAAAGAATATCTGCTCAATCGTGGGGCGAGAGTGAGTTTGGTG
GGCGTCCCGTTTAAAAGGCACACTTCTTTAATCCAAGTGGCCAAAAATTTAAACTTGAGTTTAGAAACCTTAAAATCCTA
CAACCACCAATTCCGCTATAACATTCTGCCTTCTAAAGACCCCACCTACACCATTTATATCCCTTATGAAAAACTCGCTC
TTTTCAAACAACGCCAGCTCAAACAAAATAAAAATATTCAAGCCAGTCCAAAAAGCCCTTTTATCACCCATGTGGTCTTG
CCTAAAGAAACCTTATCTTCTATCGCTAAACGCTATCAAGTCAGCATTTCCAGTATCCAATTAGCCAATAACCTCAAAGA
TTCTAACATTTTTATCCACCAGCGCTTAATCATCCCCACCAATAAGAAATTACTCGCTACAAGGGAATTTTAA

Upstream 100 bases:

>100_bases
AAATGGGGCTGTGTTAGAAAGCGTTATTCAAGTTTAGTTATAATCAAAGCTATTTTAAAAGCTGAATAGCTGTAGTTATT
AGGATGTGATGCCAAAAAGA

Downstream 100 bases:

>100_bases
TGGGTTTGGCGTTGGAAAAAGCTTGTTTTTTAGGCGTTATTTTTTTGATTAGTGCTTGCGCGGTTAAAAAAGAGGGGGTA
AAGAATTTGTCTTACAAGCA

Product: putative regulatory protein

Products: 1,6-Anhydrobond In The Muramic Acid Residue [C]

Alternate protein names: Murein hydrolase D; Regulatory protein dniR [H]

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKCFSQKWLVFFVTRWLLLASLGHAKMAFESNIDTKALEAFGVNAGFLSQMPNALKKMNKEEEWKKLVKRFDVNYQFIPI
IKNMLIKASVPQEFLFLAMAESKFSSRAYSRKKAVGIWQFMPSTAKELGLKVNHYIDERRDPIKSTQAAITYLKRLYKQT
GEWYLVAMAYNYGLRKVQNAIKAAGTSDIKILLDEDKKYLPKETREYIRSILSLALKFNSLDNLKDKEYLLNRGARVSLV
GVPFKRHTSLIQVAKNLNLSLETLKSYNHQFRYNILPSKDPTYTIYIPYEKLALFKQRQLKQNKNIQASPKSPFITHVVL
PKETLSSIAKRYQVSISSIQLANNLKDSNIFIHQRLIIPTNKKLLATREF

Sequences:

>Translated_370_residues
MKCFSQKWLVFFVTRWLLLASLGHAKMAFESNIDTKALEAFGVNAGFLSQMPNALKKMNKEEEWKKLVKRFDVNYQFIPI
IKNMLIKASVPQEFLFLAMAESKFSSRAYSRKKAVGIWQFMPSTAKELGLKVNHYIDERRDPIKSTQAAITYLKRLYKQT
GEWYLVAMAYNYGLRKVQNAIKAAGTSDIKILLDEDKKYLPKETREYIRSILSLALKFNSLDNLKDKEYLLNRGARVSLV
GVPFKRHTSLIQVAKNLNLSLETLKSYNHQFRYNILPSKDPTYTIYIPYEKLALFKQRQLKQNKNIQASPKSPFITHVVL
PKETLSSIAKRYQVSISSIQLANNLKDSNIFIHQRLIIPTNKKLLATREF
>Mature_370_residues
MKCFSQKWLVFFVTRWLLLASLGHAKMAFESNIDTKALEAFGVNAGFLSQMPNALKKMNKEEEWKKLVKRFDVNYQFIPI
IKNMLIKASVPQEFLFLAMAESKFSSRAYSRKKAVGIWQFMPSTAKELGLKVNHYIDERRDPIKSTQAAITYLKRLYKQT
GEWYLVAMAYNYGLRKVQNAIKAAGTSDIKILLDEDKKYLPKETREYIRSILSLALKFNSLDNLKDKEYLLNRGARVSLV
GVPFKRHTSLIQVAKNLNLSLETLKSYNHQFRYNILPSKDPTYTIYIPYEKLALFKQRQLKQNKNIQASPKSPFITHVVL
PKETLSSIAKRYQVSISSIQLANNLKDSNIFIHQRLIIPTNKKLLATREF

Specific function: Murein-degrading enzyme. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Cell membrane; Lipid-anchor (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 LysM repeats [H]

Homologues:

Organism=Escherichia coli, GI1786405, Length=296, Percent_Identity=31.0810810810811, Blast_Score=110, Evalue=1e-25,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008258
- InterPro:   IPR010511
- InterPro:   IPR018392
- InterPro:   IPR002482
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01476 LysM; PF06474 MLTD_N; PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 42906; Mature: 42906

Theoretical pI: Translated: 10.61; Mature: 10.61

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.2 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKCFSQKWLVFFVTRWLLLASLGHAKMAFESNIDTKALEAFGVNAGFLSQMPNALKKMNK
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCC
EEEWKKLVKRFDVNYQFIPIIKNMLIKASVPQEFLFLAMAESKFSSRAYSRKKAVGIWQF
HHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MPSTAKELGLKVNHYIDERRDPIKSTQAAITYLKRLYKQTGEWYLVAMAYNYGLRKVQNA
CCHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHH
IKAAGTSDIKILLDEDKKYLPKETREYIRSILSLALKFNSLDNLKDKEYLLNRGARVSLV
HHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEE
GVPFKRHTSLIQVAKNLNLSLETLKSYNHQFRYNILPSKDPTYTIYIPYEKLALFKQRQL
ECCHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEECCCCCCCEEEEECHHHHHHHHHHHH
KQNKNIQASPKSPFITHVVLPKETLSSIAKRYQVSISSIQLANNLKDSNIFIHQRLIIPT
HHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECC
NKKLLATREF
CCCEEEECCC
>Mature Secondary Structure
MKCFSQKWLVFFVTRWLLLASLGHAKMAFESNIDTKALEAFGVNAGFLSQMPNALKKMNK
CCCCHHHHHHHHHHHHHHHHHCCCHHHHHHCCCCHHHHHHHCCCHHHHHHHHHHHHHCCC
EEEWKKLVKRFDVNYQFIPIIKNMLIKASVPQEFLFLAMAESKFSSRAYSRKKAVGIWQF
HHHHHHHHHHHCCCCEEHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MPSTAKELGLKVNHYIDERRDPIKSTQAAITYLKRLYKQTGEWYLVAMAYNYGLRKVQNA
CCHHHHHHCCCHHHHHHHHCCHHHHHHHHHHHHHHHHHCCCCEEEEEEEHHHHHHHHHHH
IKAAGTSDIKILLDEDKKYLPKETREYIRSILSLALKFNSLDNLKDKEYLLNRGARVSLV
HHHCCCCCEEEEEECCCCCCCHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCCCCEEEEE
GVPFKRHTSLIQVAKNLNLSLETLKSYNHQFRYNILPSKDPTYTIYIPYEKLALFKQRQL
ECCHHHHHHHHHHHHCCCCHHHHHHHCCCEEEEEECCCCCCCEEEEECHHHHHHHHHHHH
KQNKNIQASPKSPFITHVVLPKETLSSIAKRYQVSISSIQLANNLKDSNIFIHQRLIIPT
HHCCCCCCCCCCCCEEEEECCHHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECC
NKKLLATREF
CCCEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]