Click here to switch to the map view.

The map label for this gene is nhaA

Identifier: 188528333

GI number: 188528333

Start: 1563341

End: 1564690

Strand: Direct

Name: nhaA

Synonym: HPSH_08025

Alternate gene names: 188528333

Gene position: 1563341-1564690 (Clockwise)

Preceding gene: 188528332

Following gene: 188528334

Centisome position: 97.19

GC content: 41.63

Gene sequence:

>1350_bases
ATGGGTTTGAAATTAAAAATTTTAAGGTTGTCTATGAATATCAAAAAAACAGAAAACGCGCTCAGTGTAACGCTTAAAAA
CTTTATTAAAAGCGAGTCTTTTGGAGGGATTTTCCTCTTTTTAAACGCTGTTTTAGCGATGGTGGTGGCTAATTCGTTTT
TAAAAGAAAGTTATTTTGCGCTATGGCACACCCCTTTTGGGTTTCAAATAGGGGATTTTTTCATCGGCTTTAGTTTGCAC
AACTGGATTGATGATGTTTTAATGGCGTTATTCTTTTTGATGATAGGCTTGGAGATCAAGCGAGAATTGTTGTTTGGGGA
GCTATCTAGTTTCAAAAAAGCTTCTTTTCCTGTGATCGCGGCCATAGGGGGCATGATAGCCCCAGGATTGATTTATTTTT
TTCTTAACGCTGACACGCCTTCTCAGCATGGTTTTGGGATCCCTATGGCGACAGATATTGCATTCGCTTTAGGCGTGATC
ATGCTTTTAGGCAAGAGGGTGCCAACCGCCTTAAAGGTTTTTTTAATCACTCTAGCGGTGGCTGATGACTTGGGGGCTAT
TGTGGTGATCGCGCTCTTTTATACCACGAATTTAAAATTCGCATGGCTTTTAGGGGCTTTAGGGGTGGTTCTTGTTTTAG
CTGTATTAAACCGCTTGAATATGCGCTCGCTTATCCCTTACTTGCTTTTAGGGGTGTTGCTTTGGTTTTGCGTGCATGAG
AGCGGTATCCATGCGACGATTGCTGCAGTGATTCTAGCTTTTATGATACCGGTGAAGATCCCTAAAGATTCTAAAAATGT
AGAGCTTTTAGAACTGGGCAAGCGATACGCAGAAACGAGTTCAGGAGCGCTTTTGACCAAAGAGCAGCAAGAAATCTTGC
ATTCTATTGAAGAAAAAGCGAGCGCTTTACAAAGCCCCTTAGAAAGATTGGAGCATTTTTTAGCCCCCATTAGCGGGTAT
TTCATCATGCCCTTGTTTGCGTTTGCAAACGCTGGGGTGAGCGTTGATTCTAGCATCAATTTAGAAGTGGATAAGGTGCT
TTTAGGGGTTATTTTAGGGCTTTGTTTGGGCAAGCCTTTAGGGATTTTCCTCATTACTTTTATAAGCGAAAAGCTTAAAA
TCACCGCGCGCCCTAAAGGCATCAGCTGGTGGCACATTTTGGGGGCTGGGCTTTTAGCAGGGATTGGCTTTACCATGTCT
ATGTTTATTTCTAATCTGGCTTTCACGAGCGAGCATAAGGACGCTATGGAAGTGGCAAAAATTGCGATTTTACTAGGATC
TTTGATTTCTGGGATCATAGGGGCTTTGTATTTATTTTTATTAAACCAAAGAGCGGCTTTAAAGAAATAG

Upstream 100 bases:

>100_bases
CGCAAGTTTCTCATTACGCTGCGTTTTTGCAAACTCAAGCCCCCCATTTTAAGATACAAGCGGGCATTATTTACGCTCAT
AAAAGACTGCTTGAAAAATT

Downstream 100 bases:

>100_bases
TTAAAAATGCTATAATTTGAGATTAAAACATCTTTTAAGGGAATTAAATGGGACAAACTAAAGACATTCTAACGACGCTT
TTACCCCTTGTGGTGCTGTT

Product: pH-dependent sodium/proton antiporter

Products: Na (I) [Periplasm]; Proton [Cytoplasm] [C]

Alternate protein names: Sodium/proton antiporter nhaA [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MGLKLKILRLSMNIKKTENALSVTLKNFIKSESFGGIFLFLNAVLAMVVANSFLKESYFALWHTPFGFQIGDFFIGFSLH
NWIDDVLMALFFLMIGLEIKRELLFGELSSFKKASFPVIAAIGGMIAPGLIYFFLNADTPSQHGFGIPMATDIAFALGVI
MLLGKRVPTALKVFLITLAVADDLGAIVVIALFYTTNLKFAWLLGALGVVLVLAVLNRLNMRSLIPYLLLGVLLWFCVHE
SGIHATIAAVILAFMIPVKIPKDSKNVELLELGKRYAETSSGALLTKEQQEILHSIEEKASALQSPLERLEHFLAPISGY
FIMPLFAFANAGVSVDSSINLEVDKVLLGVILGLCLGKPLGIFLITFISEKLKITARPKGISWWHILGAGLLAGIGFTMS
MFISNLAFTSEHKDAMEVAKIAILLGSLISGIIGALYLFLLNQRAALKK

Sequences:

>Translated_449_residues
MGLKLKILRLSMNIKKTENALSVTLKNFIKSESFGGIFLFLNAVLAMVVANSFLKESYFALWHTPFGFQIGDFFIGFSLH
NWIDDVLMALFFLMIGLEIKRELLFGELSSFKKASFPVIAAIGGMIAPGLIYFFLNADTPSQHGFGIPMATDIAFALGVI
MLLGKRVPTALKVFLITLAVADDLGAIVVIALFYTTNLKFAWLLGALGVVLVLAVLNRLNMRSLIPYLLLGVLLWFCVHE
SGIHATIAAVILAFMIPVKIPKDSKNVELLELGKRYAETSSGALLTKEQQEILHSIEEKASALQSPLERLEHFLAPISGY
FIMPLFAFANAGVSVDSSINLEVDKVLLGVILGLCLGKPLGIFLITFISEKLKITARPKGISWWHILGAGLLAGIGFTMS
MFISNLAFTSEHKDAMEVAKIAILLGSLISGIIGALYLFLLNQRAALKK
>Mature_448_residues
GLKLKILRLSMNIKKTENALSVTLKNFIKSESFGGIFLFLNAVLAMVVANSFLKESYFALWHTPFGFQIGDFFIGFSLHN
WIDDVLMALFFLMIGLEIKRELLFGELSSFKKASFPVIAAIGGMIAPGLIYFFLNADTPSQHGFGIPMATDIAFALGVIM
LLGKRVPTALKVFLITLAVADDLGAIVVIALFYTTNLKFAWLLGALGVVLVLAVLNRLNMRSLIPYLLLGVLLWFCVHES
GIHATIAAVILAFMIPVKIPKDSKNVELLELGKRYAETSSGALLTKEQQEILHSIEEKASALQSPLERLEHFLAPISGYF
IMPLFAFANAGVSVDSSINLEVDKVLLGVILGLCLGKPLGIFLITFISEKLKITARPKGISWWHILGAGLLAGIGFTMSM
FISNLAFTSEHKDAMEVAKIAILLGSLISGIIGALYLFLLNQRAALKK

Specific function: Na(+)/H(+) antiporter that extrudes sodium in exchange for external protons [H]

COG id: COG3004

COG function: function code P; Na+/H+ antiporter

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the nhaA Na(+)/H(+) (TC 2.A.33) antiporter family [H]

Homologues:

Organism=Escherichia coli, GI1786201, Length=417, Percent_Identity=45.5635491606715, Blast_Score=290, Evalue=2e-79,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR023171
- InterPro:   IPR004670 [H]

Pfam domain/function: PF06965 Na_H_antiport_1 [H]

EC number: NA

Molecular weight: Translated: 49057; Mature: 48925

Theoretical pI: Translated: 9.58; Mature: 9.58

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGLKLKILRLSMNIKKTENALSVTLKNFIKSESFGGIFLFLNAVLAMVVANSFLKESYFA
CCCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LWHTPFGFQIGDFFIGFSLHNWIDDVLMALFFLMIGLEIKRELLFGELSSFKKASFPVIA
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AIGGMIAPGLIYFFLNADTPSQHGFGIPMATDIAFALGVIMLLGKRVPTALKVFLITLAV
HHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ADDLGAIVVIALFYTTNLKFAWLLGALGVVLVLAVLNRLNMRSLIPYLLLGVLLWFCVHE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
SGIHATIAAVILAFMIPVKIPKDSKNVELLELGKRYAETSSGALLTKEQQEILHSIEEKA
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHH
SALQSPLERLEHFLAPISGYFIMPLFAFANAGVSVDSSINLEVDKVLLGVILGLCLGKPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHH
GIFLITFISEKLKITARPKGISWWHILGAGLLAGIGFTMSMFISNLAFTSEHKDAMEVAK
HHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IAILLGSLISGIIGALYLFLLNQRAALKK
HHHHHHHHHHHHHHHHHHHHHCCHHHCCC
>Mature Secondary Structure 
GLKLKILRLSMNIKKTENALSVTLKNFIKSESFGGIFLFLNAVLAMVVANSFLKESYFA
CCEEEEEEEECCCHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHH
LWHTPFGFQIGDFFIGFSLHNWIDDVLMALFFLMIGLEIKRELLFGELSSFKKASFPVIA
HCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHH
AIGGMIAPGLIYFFLNADTPSQHGFGIPMATDIAFALGVIMLLGKRVPTALKVFLITLAV
HHHHHHHHHHHEEEECCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
ADDLGAIVVIALFYTTNLKFAWLLGALGVVLVLAVLNRLNMRSLIPYLLLGVLLWFCVHE
HHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCC
SGIHATIAAVILAFMIPVKIPKDSKNVELLELGKRYAETSSGALLTKEQQEILHSIEEKA
CCHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHCCCCCCEEEHHHHHHHHHHHHHH
SALQSPLERLEHFLAPISGYFIMPLFAFANAGVSVDSSINLEVDKVLLGVILGLCLGKPL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHCCHH
GIFLITFISEKLKITARPKGISWWHILGAGLLAGIGFTMSMFISNLAFTSEHKDAMEVAK
HHHHHHHHHHHHEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHH
IAILLGSLISGIIGALYLFLLNQRAALKK
HHHHHHHHHHHHHHHHHHHHHCCHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Na (I) [Cytoplasm]; Proton [Periplasm] [C]

Specific reaction: Na (I) [Cytoplasm] + 2 Proton [Periplasm] = Na (I) [Periplasm] + 2 Proton [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: NA