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The map label for this gene is lysC [H]

Identifier: 188528016

GI number: 188528016

Start: 1253413

End: 1254630

Strand: Direct

Name: lysC [H]

Synonym: HPSH_06355

Alternate gene names: 188528016

Gene position: 1253413-1254630 (Clockwise)

Preceding gene: 188528015

Following gene: 188528017

Centisome position: 77.92

GC content: 44.99

Gene sequence:

>1218_bases
GTGTTAATCGTTCAAAAATACGGCGGCACGAGCATGGGCAGCGTAGAAAGGATCCACAATGTCGCTCAAAGGGTTTTAGA
AAGCGTTAAATTAGGGCATCAGGTGGTGGTGGTGGTTTCGGCGATGAGCGGCGAAACCGATAGGCTTTTAGAATTTGGCA
AGAATTTTAGCCATAACCCTAACAAGCGAGAAATGGACAGGATTGTAAGCGCGGGGGAATTGATTTCAAGCGCGGCTTTA
AGTATGGCGTTAGAAAGGTATGGGCATAGAGCCATTTCATTGAGCGGGAAAGAAGCGGGCATTTTAACCAGTTCGCATTT
TCAAAACGCCGTGATCCAGTCCATTGACACCAAACACATCACAGAGCTTTTAGAAAAAAACTACATTGTGGTGATCGCTG
GGTTTCAAGGCGCTGATATTCAAGGCGAAACAACGACTTTAGGGCGTGGGGGGAGCGATTTGAGCGCGGTCGCTTTGGCC
GGGGCTTTAAAAGCGCATTTGTGCGAAATCTATACGGATGTGGATGGCGTTTATACCACTGATCCGCGCATTGAAGAAAA
AGCTCAAAAAATCGCGCAAATCAGCTATGATGAAATGCTTGAACTGGCTTCTATGGGGGCTAAAGTTTTATTGAACCGAT
CGGTGGAATTGGCCAAAAAACTCAGCGTCAAGTTAGTGACTCGCAATTCGTTTAACCATAGCGAAGGCACGCTCATTGTG
GCTGAAAAAGACTTTAAAGGAGAACGCATGGAAACCCCTATAGTGAGTGGGATCGCATTGGATAAAAATCAGGCTCGTGT
GAGCATGGAGGGCGTGGAAGACAGGCCAGGCATTGCCGCTGAAATCTTTGGCGCTTTAGCGGAGTATCGCATTAATGTGG
ATATGATCGTCCAAACGATCGGCAGAGACGGCAAAACCGATTTGGATTTTACGATCGTTAAAACCCAAATAGAAGAAACC
AAGCAAGCCTTAAAGCCTTTTTTAGCGCAAATGGATTCCATTGATTATGATGAAAATATCGCTAAAGTTTCCATAGTGGG
CGTGGGCATGAAGTCGCATTCTGGGGTAGCGAGTACCGCTTTTAAAGCCCTAGCCAAAGACAATATCAATATCATGATGA
TTTCTACAAGCGAGATTAAAATTTCGGTTTTGATTGACATTAAATACGCTGAATTAGCCGTTAGAACTTTGCATGCGGTG
TATCAATTGGATCAATGA

Upstream 100 bases:

>100_bases
AATTCATCCATCTTAAGGATTTGCTTAAAAAAATCGTTCCCTTTAAGCGTCAAGTGTATCGCCAAGTCATTGCTTATTTC
AGAAGAGAGGGGTATTTAGG

Downstream 100 bases:

>100_bases
AAAATTTCTACGACTGGATTAAGGAATTTGTGCGCGATCAAGGGGAATTTATCGCCCAACAAAGCGGGTGGCTGGAATTG
GAGCGTTCAAGCTATGCCAA

Product: aspartate kinase

Products: NA

Alternate protein names: Aspartate kinase [H]

Number of amino acids: Translated: 405; Mature: 405

Protein sequence:

>405_residues
MLIVQKYGGTSMGSVERIHNVAQRVLESVKLGHQVVVVVSAMSGETDRLLEFGKNFSHNPNKREMDRIVSAGELISSAAL
SMALERYGHRAISLSGKEAGILTSSHFQNAVIQSIDTKHITELLEKNYIVVIAGFQGADIQGETTTLGRGGSDLSAVALA
GALKAHLCEIYTDVDGVYTTDPRIEEKAQKIAQISYDEMLELASMGAKVLLNRSVELAKKLSVKLVTRNSFNHSEGTLIV
AEKDFKGERMETPIVSGIALDKNQARVSMEGVEDRPGIAAEIFGALAEYRINVDMIVQTIGRDGKTDLDFTIVKTQIEET
KQALKPFLAQMDSIDYDENIAKVSIVGVGMKSHSGVASTAFKALAKDNINIMMISTSEIKISVLIDIKYAELAVRTLHAV
YQLDQ

Sequences:

>Translated_405_residues
MLIVQKYGGTSMGSVERIHNVAQRVLESVKLGHQVVVVVSAMSGETDRLLEFGKNFSHNPNKREMDRIVSAGELISSAAL
SMALERYGHRAISLSGKEAGILTSSHFQNAVIQSIDTKHITELLEKNYIVVIAGFQGADIQGETTTLGRGGSDLSAVALA
GALKAHLCEIYTDVDGVYTTDPRIEEKAQKIAQISYDEMLELASMGAKVLLNRSVELAKKLSVKLVTRNSFNHSEGTLIV
AEKDFKGERMETPIVSGIALDKNQARVSMEGVEDRPGIAAEIFGALAEYRINVDMIVQTIGRDGKTDLDFTIVKTQIEET
KQALKPFLAQMDSIDYDENIAKVSIVGVGMKSHSGVASTAFKALAKDNINIMMISTSEIKISVLIDIKYAELAVRTLHAV
YQLDQ
>Mature_405_residues
MLIVQKYGGTSMGSVERIHNVAQRVLESVKLGHQVVVVVSAMSGETDRLLEFGKNFSHNPNKREMDRIVSAGELISSAAL
SMALERYGHRAISLSGKEAGILTSSHFQNAVIQSIDTKHITELLEKNYIVVIAGFQGADIQGETTTLGRGGSDLSAVALA
GALKAHLCEIYTDVDGVYTTDPRIEEKAQKIAQISYDEMLELASMGAKVLLNRSVELAKKLSVKLVTRNSFNHSEGTLIV
AEKDFKGERMETPIVSGIALDKNQARVSMEGVEDRPGIAAEIFGALAEYRINVDMIVQTIGRDGKTDLDFTIVKTQIEET
KQALKPFLAQMDSIDYDENIAKVSIVGVGMKSHSGVASTAFKALAKDNINIMMISTSEIKISVLIDIKYAELAVRTLHAV
YQLDQ

Specific function: Catalyzes 2 nonconsecutive reactions in the common biosynthetic pathway leading from Asp to diaminopimelate and Lys, to Met, and to Thr and Ile. [C]

COG id: COG0527

COG function: function code E; Aspartokinases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 2 ACT domains [H]

Homologues:

Organism=Escherichia coli, GI1786183, Length=351, Percent_Identity=32.7635327635328, Blast_Score=164, Evalue=7e-42,
Organism=Escherichia coli, GI1790455, Length=455, Percent_Identity=27.2527472527473, Blast_Score=119, Evalue=4e-28,
Organism=Escherichia coli, GI1790376, Length=172, Percent_Identity=29.6511627906977, Blast_Score=69, Evalue=4e-13,
Organism=Saccharomyces cerevisiae, GI6320893, Length=489, Percent_Identity=24.9488752556237, Blast_Score=94, Evalue=4e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002912
- InterPro:   IPR001048
- InterPro:   IPR005260
- InterPro:   IPR001341
- InterPro:   IPR018042 [H]

Pfam domain/function: PF00696 AA_kinase; PF01842 ACT [H]

EC number: =2.7.2.4 [H]

Molecular weight: Translated: 44102; Mature: 44102

Theoretical pI: Translated: 6.03; Mature: 6.03

Prosite motif: PS00324 ASPARTOKINASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLIVQKYGGTSMGSVERIHNVAQRVLESVKLGHQVVVVVSAMSGETDRLLEFGKNFSHNP
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHCCCCCCCC
NKREMDRIVSAGELISSAALSMALERYGHRAISLSGKEAGILTSSHFQNAVIQSIDTKHI
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEHHHHHHHHHHHCCHHHH
TELLEKNYIVVIAGFQGADIQGETTTLGRGGSDLSAVALAGALKAHLCEIYTDVDGVYTT
HHHHHCCEEEEEECCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEC
DPRIEEKAQKIAQISYDEMLELASMGAKVLLNRSVELAKKLSVKLVTRNSFNHSEGTLIV
CCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHCCHHHHHHCEEEEEEECCCCCCCCEEEE
AEKDFKGERMETPIVSGIALDKNQARVSMEGVEDRPGIAAEIFGALAEYRINVDMIVQTI
EECCCCCCCCCCCCCCCEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
GRDGKTDLDFTIVKTQIEETKQALKPFLAQMDSIDYDENIAKVSIVGVGMKSHSGVASTA
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHH
FKALAKDNINIMMISTSEIKISVLIDIKYAELAVRTLHAVYQLDQ
HHHHHHCCCEEEEEECCCEEEEEEEEEHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLIVQKYGGTSMGSVERIHNVAQRVLESVKLGHQVVVVVSAMSGETDRLLEFGKNFSHNP
CEEEEECCCCCCHHHHHHHHHHHHHHHHHHCCCEEEEEEEECCCCHHHHHHHCCCCCCCC
NKREMDRIVSAGELISSAALSMALERYGHRAISLSGKEAGILTSSHFQNAVIQSIDTKHI
CHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEECCCCCCEEEHHHHHHHHHHHCCHHHH
TELLEKNYIVVIAGFQGADIQGETTTLGRGGSDLSAVALAGALKAHLCEIYTDVDGVYTT
HHHHHCCEEEEEECCCCCCCCCCCEECCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEC
DPRIEEKAQKIAQISYDEMLELASMGAKVLLNRSVELAKKLSVKLVTRNSFNHSEGTLIV
CCCHHHHHHHHHHCCHHHHHHHHHCCHHHHHHCCHHHHHHCEEEEEEECCCCCCCCEEEE
AEKDFKGERMETPIVSGIALDKNQARVSMEGVEDRPGIAAEIFGALAEYRINVDMIVQTI
EECCCCCCCCCCCCCCCEEECCCCCEEEECCCCCCCCHHHHHHHHHHHHCCCHHHHHHHH
GRDGKTDLDFTIVKTQIEETKQALKPFLAQMDSIDYDENIAKVSIVGVGMKSHSGVASTA
CCCCCCCCEEEEEHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEECCCCCCCHHHHH
FKALAKDNINIMMISTSEIKISVLIDIKYAELAVRTLHAVYQLDQ
HHHHHHCCCEEEEEECCCEEEEEEEEEHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]