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The map label for this gene is hisS

Identifier: 188527977

GI number: 188527977

Start: 1211733

End: 1213061

Strand: Reverse

Name: hisS

Synonym: HPSH_06160

Alternate gene names: 188527977

Gene position: 1213061-1211733 (Counterclockwise)

Preceding gene: 188527979

Following gene: 188527976

Centisome position: 75.41

GC content: 38.98

Gene sequence:

>1329_bases
ATGATCACCCCTAAAGTGTTGAGCGGGTTTAAAGACCGCTTGCCTAAAGATGCGATACAAAAAGCCCAGTTGCTTTCTAA
AGTTTCGGTCGTGTTTCAAAGTTTTGGTTTTGTGCCGATTGAAACCCCTCATTTGGAATACGCTGAAGTGTTATTGCCTG
ATGCGAGCAGTGATATTCAAAAAGAAATTTATCGTTTTAAAGACCATGGGGATAGGGATGTGGCTTTAAGGTTTGATTTG
ACCGTGCCATTAGCCCGCTTTGTTTCTTTGCACCACCAAACGCTAGGCATGCCCTTCAAACGCTACGCTATAGGCAATGT
CTTTAGGGGCGAAAGGGCGCAAAAAGGGCGTTATAGGGAATTTACGCAATGCGATTTTGATTTTATAGGGAGCGAAAGCT
TGGTGTGCGATGCTGAGATCATTCAAGTGATTGTCGCTTCTTTAAAAGCTTTGGATTTAGAAGATTTTTGCATCTCTATC
AACCACAGAAAAATTTTGAACGGGATATGCGAATATTTTGGCGTTTCTCAAGTGAATGAAGCGTTGCGCATTGTGGATAA
ATTAGAAAAGATTGGCCTAAATGGGGTTGAAGAAGAATTAAAAAAAGAGTGCGATTTGGATTTAAACACCATTAAAGAGC
TTTTAGAAATGGTTCAAATCAAACAAAACGATTTAAGCCATGCGGAATTTTTTGAAAAAATTGCTTATTTGAAAGACTAT
AATGAAAATCTGAAAAAGGGCATACAGGATTTAGAAAGGCTATACCAGTTGCTAGGGGATTTGCAAATTTCTCAAAACCT
GTATAAAATTGATTTTTCTATCGCTAGGGGATTAGGGTATTATACAGGGATTGTGTATGAAACCACGCTTAATGGCATGA
AGTCTTTAGGGAGCGTGTGTTCAGGGGGTCGTTACGATCATTTGACTCAAAATTTTTCTAAAGAGAATTTACAAGGGGTG
GGGGCTTCTATTGGGATTGATCGATTGATTGTGGCTTTGAGTGAAATGCAATTACTAGACGAGCGCTCCACCCAAGCTAA
AGTCTTAATCGCTTGCATGCATGAAGAGTATTTTTCTTATGCAAACCGCTTAGCGGAGTCTTTAAGGCAAAGCGGGATTT
TTAGCGAAGTCTATCCAGAAGCTCAAAAAATCAAAAAACCCTTTTCTTATGCTAATCATAAAGGGCATGAGTTCGTGGCT
GTCATTGGCGAAGAAGAATTTAAAAGCGAAACCTTAAGCTTGAAAAACATGCATTCAGGCATGCAGTTGAATTGTTTGAG
TTTTTTAAAAGCCCTTGAAATCATTGGAGAAAACGATGAAGACTTATAA

Upstream 100 bases:

>100_bases
CAATAAAATACGCATGCGTTTGGGCGCATTTACGCTCATCAATAGCCCTTTTTTAAAGATTTTAAGCTAAAATTTTAGCA
TACAAATACAAGGAAATGGA

Downstream 100 bases:

>100_bases
TGTCGCTATTGTTGGGGCTAGTGGGGCGGTAGGCCAAGAGCTGATTAAGGGTTTAGAAAATTCTTCTTTCCCGATTAAAA
AATTTGTCCCGCTCGCTAGT

Product: histidyl-tRNA synthetase

Products: NA

Alternate protein names: Histidine--tRNA ligase; HisRS [H]

Number of amino acids: Translated: 442; Mature: 442

Protein sequence:

>442_residues
MITPKVLSGFKDRLPKDAIQKAQLLSKVSVVFQSFGFVPIETPHLEYAEVLLPDASSDIQKEIYRFKDHGDRDVALRFDL
TVPLARFVSLHHQTLGMPFKRYAIGNVFRGERAQKGRYREFTQCDFDFIGSESLVCDAEIIQVIVASLKALDLEDFCISI
NHRKILNGICEYFGVSQVNEALRIVDKLEKIGLNGVEEELKKECDLDLNTIKELLEMVQIKQNDLSHAEFFEKIAYLKDY
NENLKKGIQDLERLYQLLGDLQISQNLYKIDFSIARGLGYYTGIVYETTLNGMKSLGSVCSGGRYDHLTQNFSKENLQGV
GASIGIDRLIVALSEMQLLDERSTQAKVLIACMHEEYFSYANRLAESLRQSGIFSEVYPEAQKIKKPFSYANHKGHEFVA
VIGEEEFKSETLSLKNMHSGMQLNCLSFLKALEIIGENDEDL

Sequences:

>Translated_442_residues
MITPKVLSGFKDRLPKDAIQKAQLLSKVSVVFQSFGFVPIETPHLEYAEVLLPDASSDIQKEIYRFKDHGDRDVALRFDL
TVPLARFVSLHHQTLGMPFKRYAIGNVFRGERAQKGRYREFTQCDFDFIGSESLVCDAEIIQVIVASLKALDLEDFCISI
NHRKILNGICEYFGVSQVNEALRIVDKLEKIGLNGVEEELKKECDLDLNTIKELLEMVQIKQNDLSHAEFFEKIAYLKDY
NENLKKGIQDLERLYQLLGDLQISQNLYKIDFSIARGLGYYTGIVYETTLNGMKSLGSVCSGGRYDHLTQNFSKENLQGV
GASIGIDRLIVALSEMQLLDERSTQAKVLIACMHEEYFSYANRLAESLRQSGIFSEVYPEAQKIKKPFSYANHKGHEFVA
VIGEEEFKSETLSLKNMHSGMQLNCLSFLKALEIIGENDEDL
>Mature_442_residues
MITPKVLSGFKDRLPKDAIQKAQLLSKVSVVFQSFGFVPIETPHLEYAEVLLPDASSDIQKEIYRFKDHGDRDVALRFDL
TVPLARFVSLHHQTLGMPFKRYAIGNVFRGERAQKGRYREFTQCDFDFIGSESLVCDAEIIQVIVASLKALDLEDFCISI
NHRKILNGICEYFGVSQVNEALRIVDKLEKIGLNGVEEELKKECDLDLNTIKELLEMVQIKQNDLSHAEFFEKIAYLKDY
NENLKKGIQDLERLYQLLGDLQISQNLYKIDFSIARGLGYYTGIVYETTLNGMKSLGSVCSGGRYDHLTQNFSKENLQGV
GASIGIDRLIVALSEMQLLDERSTQAKVLIACMHEEYFSYANRLAESLRQSGIFSEVYPEAQKIKKPFSYANHKGHEFVA
VIGEEEFKSETLSLKNMHSGMQLNCLSFLKALEIIGENDEDL

Specific function: Unknown

COG id: COG0124

COG function: function code J; Histidyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI6996014, Length=436, Percent_Identity=32.3394495412844, Blast_Score=190, Evalue=2e-48,
Organism=Homo sapiens, GI15029520, Length=436, Percent_Identity=30.9633027522936, Blast_Score=181, Evalue=2e-45,
Organism=Homo sapiens, GI65287717, Length=416, Percent_Identity=25.7211538461538, Blast_Score=82, Evalue=1e-15,
Organism=Escherichia coli, GI1788861, Length=423, Percent_Identity=25.531914893617, Blast_Score=118, Evalue=7e-28,
Organism=Caenorhabditis elegans, GI71993686, Length=440, Percent_Identity=32.7272727272727, Blast_Score=201, Evalue=6e-52,
Organism=Caenorhabditis elegans, GI71993693, Length=440, Percent_Identity=32.7272727272727, Blast_Score=201, Evalue=7e-52,
Organism=Saccharomyces cerevisiae, GI6325290, Length=457, Percent_Identity=30.6345733041575, Blast_Score=183, Evalue=6e-47,
Organism=Saccharomyces cerevisiae, GI6320489, Length=311, Percent_Identity=23.1511254019293, Blast_Score=72, Evalue=2e-13,
Organism=Drosophila melanogaster, GI24643061, Length=459, Percent_Identity=30.5010893246187, Blast_Score=173, Evalue=2e-43,
Organism=Drosophila melanogaster, GI24643059, Length=478, Percent_Identity=29.2887029288703, Blast_Score=162, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002314
- InterPro:   IPR006195
- InterPro:   IPR004154
- InterPro:   IPR015807
- InterPro:   IPR004516 [H]

Pfam domain/function: PF03129 HGTP_anticodon; PF00587 tRNA-synt_2b [H]

EC number: =6.1.1.21 [H]

Molecular weight: Translated: 50205; Mature: 50205

Theoretical pI: Translated: 5.26; Mature: 5.26

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITPKVLSGFKDRLPKDAIQKAQLLSKVSVVFQSFGFVPIETPHLEYAEVLLPDASSDIQ
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCHHHH
KEIYRFKDHGDRDVALRFDLTVPLARFVSLHHQTLGMPFKRYAIGNVFRGERAQKGRYRE
HHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHCCHHCCCHHCCCCCCH
FTQCDFDFIGSESLVCDAEIIQVIVASLKALDLEDFCISINHRKILNGICEYFGVSQVNE
HHHCCCHHCCCCCCEECHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHCHHHHHH
ALRIVDKLEKIGLNGVEEELKKECDLDLNTIKELLEMVQIKQNDLSHAEFFEKIAYLKDY
HHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
NENLKKGIQDLERLYQLLGDLQISQNLYKIDFSIARGLGYYTGIVYETTLNGMKSLGSVC
HHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SGGRYDHLTQNFSKENLQGVGASIGIDRLIVALSEMQLLDERSTQAKVLIACMHEEYFSY
CCCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ANRLAESLRQSGIFSEVYPEAQKIKKPFSYANHKGHEFVAVIGEEEFKSETLSLKNMHSG
HHHHHHHHHHCCCHHHHCCCHHHHHCCHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCC
MQLNCLSFLKALEIIGENDEDL
CCHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MITPKVLSGFKDRLPKDAIQKAQLLSKVSVVFQSFGFVPIETPHLEYAEVLLPDASSDIQ
CCCHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHCCCCCHHHH
KEIYRFKDHGDRDVALRFDLTVPLARFVSLHHQTLGMPFKRYAIGNVFRGERAQKGRYRE
HHHHHHHCCCCCEEEEEEECHHHHHHHHHHHHHHHCCCHHHHHHCCHHCCCHHCCCCCCH
FTQCDFDFIGSESLVCDAEIIQVIVASLKALDLEDFCISINHRKILNGICEYFGVSQVNE
HHHCCCHHCCCCCCEECHHHHHHHHHHHHHCCHHHHHHCCCHHHHHHHHHHHHCHHHHHH
ALRIVDKLEKIGLNGVEEELKKECDLDLNTIKELLEMVQIKQNDLSHAEFFEKIAYLKDY
HHHHHHHHHHCCCCCHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHH
NENLKKGIQDLERLYQLLGDLQISQNLYKIDFSIARGLGYYTGIVYETTLNGMKSLGSVC
HHHHHHHHHHHHHHHHHHCCHHHHCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHH
SGGRYDHLTQNFSKENLQGVGASIGIDRLIVALSEMQLLDERSTQAKVLIACMHEEYFSY
CCCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
ANRLAESLRQSGIFSEVYPEAQKIKKPFSYANHKGHEFVAVIGEEEFKSETLSLKNMHSG
HHHHHHHHHHCCCHHHHCCCHHHHHCCHHHCCCCCCEEEEEECCHHHHHHHHHHHHHCCC
MQLNCLSFLKALEIIGENDEDL
CCHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA