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The map label for this gene is trmD

Identifier: 188527932

GI number: 188527932

Start: 1163799

End: 1164488

Strand: Reverse

Name: trmD

Synonym: HPSH_05925

Alternate gene names: 188527932

Gene position: 1164488-1163799 (Counterclockwise)

Preceding gene: 188527933

Following gene: 188527931

Centisome position: 72.39

GC content: 40.0

Gene sequence:

>690_bases
GTGAAATTCAGCGTTTTAACCCTTTTCCCGCAACTCGTTTTACCCTATTTTGAGGACTCCATTTTAAAAAGAGCGTTAGA
AAAAAATCTTTTTGAATTAGAAGTGTTGAACCTTAGGGATTTTAGCGCTAATAAACATCAAAAAGCGGATCACACGCTCA
TTGGTGGGGGTGCAGGGCAAATTTTAGACCCTGAGATGGTAGAAAACGCGCTCCATTCTGTTAAAAACCCTAAACACACG
ATTTTTTTAAGCGCGGTGGGCAAGCCTTTCAAGCAAACAGACGCAATGCGTTTGGCTCAAAAAAAGCATGTCGTTTTGGT
GTGCGGGCGTTATGAGGGCTTTGATGAACGCTCTATTGAACTTGGTGCTGATGAGGTTTTTTGTATAGGCGATTTTATTT
TAACAGGGGGCGAGCTTGGGGCGTTGTGCTTGATAGATAGTATCGCTCGCCACATTCAAGGGGTTTTGGGTAACGCTCAA
TCTTTAGAAAATGAGAGCTTTGAAAACCATTATTTGGAAGCCCCTAATTTCGCCAACGCTGTTTTCAAATCCAAAGAAAT
CAATAAAATCCCTGCACCTTTAGAATATTCTAAAGGAAATCATGCTAGAATCAAGCGACTAAAACTTGATTTGTCAAAAT
TAAGGACAAAATTTTACCGCCTTGATTTATTCAAACAGCACAAATCATGA

Upstream 100 bases:

>100_bases
TTTTTTAATCCCTTATAGGGATTTTTATATCAAAGAAATCCTTTTGCAAGACAAAAAAATAACCACCAATAACGCTAAAA
CGCTTTTAGAGAATAGTTGA

Downstream 100 bases:

>100_bases
AATTGACATTAAGGAAAACACATGAAAAACCGCTACATTCAGCAGTTTGAAGACGCTCAATTAAAAGACAAGACCATGCC
AGCATTTAAAGCCGGCGATA

Product: tRNA (guanine-N(1)-)-methyltransferase

Products: NA

Alternate protein names: M1G-methyltransferase; tRNA [GM37] methyltransferase [H]

Number of amino acids: Translated: 229; Mature: 229

Protein sequence:

>229_residues
MKFSVLTLFPQLVLPYFEDSILKRALEKNLFELEVLNLRDFSANKHQKADHTLIGGGAGQILDPEMVENALHSVKNPKHT
IFLSAVGKPFKQTDAMRLAQKKHVVLVCGRYEGFDERSIELGADEVFCIGDFILTGGELGALCLIDSIARHIQGVLGNAQ
SLENESFENHYLEAPNFANAVFKSKEINKIPAPLEYSKGNHARIKRLKLDLSKLRTKFYRLDLFKQHKS

Sequences:

>Translated_229_residues
MKFSVLTLFPQLVLPYFEDSILKRALEKNLFELEVLNLRDFSANKHQKADHTLIGGGAGQILDPEMVENALHSVKNPKHT
IFLSAVGKPFKQTDAMRLAQKKHVVLVCGRYEGFDERSIELGADEVFCIGDFILTGGELGALCLIDSIARHIQGVLGNAQ
SLENESFENHYLEAPNFANAVFKSKEINKIPAPLEYSKGNHARIKRLKLDLSKLRTKFYRLDLFKQHKS
>Mature_229_residues
MKFSVLTLFPQLVLPYFEDSILKRALEKNLFELEVLNLRDFSANKHQKADHTLIGGGAGQILDPEMVENALHSVKNPKHT
IFLSAVGKPFKQTDAMRLAQKKHVVLVCGRYEGFDERSIELGADEVFCIGDFILTGGELGALCLIDSIARHIQGVLGNAQ
SLENESFENHYLEAPNFANAVFKSKEINKIPAPLEYSKGNHARIKRLKLDLSKLRTKFYRLDLFKQHKS

Specific function: Specifically methylates guanosine-37 in various tRNAs [H]

COG id: COG0336

COG function: function code J; tRNA-(guanine-N1)-methyltransferase

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the RNA methyltransferase trmD family [H]

Homologues:

Organism=Escherichia coli, GI1788959, Length=229, Percent_Identity=33.6244541484716, Blast_Score=138, Evalue=3e-34,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016009
- InterPro:   IPR002649
- InterPro:   IPR023148 [H]

Pfam domain/function: PF01746 tRNA_m1G_MT [H]

EC number: =2.1.1.31 [H]

Molecular weight: Translated: 25836; Mature: 25836

Theoretical pI: Translated: 8.80; Mature: 8.80

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKFSVLTLFPQLVLPYFEDSILKRALEKNLFELEVLNLRDFSANKHQKADHTLIGGGAGQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCC
ILDPEMVENALHSVKNPKHTIFLSAVGKPFKQTDAMRLAQKKHVVLVCGRYEGFDERSIE
CCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCEE
LGADEVFCIGDFILTGGELGALCLIDSIARHIQGVLGNAQSLENESFENHYLEAPNFANA
CCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHCCCCCCHHHH
VFKSKEINKIPAPLEYSKGNHARIKRLKLDLSKLRTKFYRLDLFKQHKS
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure
MKFSVLTLFPQLVLPYFEDSILKRALEKNLFELEVLNLRDFSANKHQKADHTLIGGGAGQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCCCCCCEEECCCCCC
ILDPEMVENALHSVKNPKHTIFLSAVGKPFKQTDAMRLAQKKHVVLVCGRYEGFDERSIE
CCCHHHHHHHHHHHCCCCEEEEEEECCCCHHHHHHHHHHHCCCEEEEEECCCCCCCCCEE
LGADEVFCIGDFILTGGELGALCLIDSIARHIQGVLGNAQSLENESFENHYLEAPNFANA
CCCCCEEEEEHHHHCCCCHHHHHHHHHHHHHHHHHHCCHHHHCCCCHHHHCCCCCCHHHH
VFKSKEINKIPAPLEYSKGNHARIKRLKLDLSKLRTKFYRLDLFKQHKS
HHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]