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The map label for this gene is pcm

Identifier: 188527871

GI number: 188527871

Start: 1105365

End: 1105994

Strand: Direct

Name: pcm

Synonym: HPSH_05600

Alternate gene names: 188527871

Gene position: 1105365-1105994 (Clockwise)

Preceding gene: 188527870

Following gene: 188527872

Centisome position: 68.72

GC content: 42.86

Gene sequence:

>630_bases
TTGAAGAGTATTAAAAACCATTTGATGTGTGAAGAAATCAATAAGCGTTTCAGTTTGCACCCCAAAGTGAGAGAAGCCAT
GGAAAGCATTGAAAGGGAAGTTTTTGTGCCAGCCCCTTTTAAACATTTTGCTTACACTTTAAACGCGCTTTCTATGCAAG
CGCAACAATACATTTCTTCGCCCCTAACCGTGGCCAAAATGACGCAATATTTAGAAATTGATCATGTGGATAGCGTGCTA
GAAATTGGCTGCGGGAGCGGCTATCAAGCGGCGGTGCTTTCTCAAATTTTCAGGCGCGTTTTTAGCATTGAAAGGATTGA
AAGCCTGTATATAGAAGCGCGTTTGCGCCTTAAAACTCTCGGTCTAAACAACGTTCATGTTAAATTCGCTGATGGGAATA
AGGGCTGGGATCAATACGCCCCCTATGATAGGATTTTGTTCTCTGCTTGCGCTAAAAATATCCCTCAAGCGCTGATTGAT
CAGCTTGAAGAAGGCGGGATATTAGTCGCGCCCATTCAAGAAAATAACGAGCAAGTGATCAAACGCTTTGTGAAGCAAAA
TAACGCCTTGCGCGTCCAAAAAGTGTTAGAGAAATGCTTGTTTGTGCCTGTTGTAGATGGGGTGCAATAA

Upstream 100 bases:

>100_bases
CTTTTCAAGTTTCAATGAGCAGCGCTCTAATTTCTTTGAAGCTAGGGTGAGTAATTACGCTAAAGGGAGCGTGAGTTTTG
ACGATTTTTAAGGGGTTTGT

Downstream 100 bases:

>100_bases
AGATTAAGGCATGATTAAACACTATCTTTTCATGGCGGTTTCGCAAGTCTTTTTCTCTTTCTTTTTAGTGCTGTTTTTCA
TCTCCTCTATCGTGCTATTA

Product: protein-L-isoaspartate O-methyltransferase

Products: NA

Alternate protein names: L-isoaspartyl protein carboxyl methyltransferase; Protein L-isoaspartyl methyltransferase; Protein-beta-aspartate methyltransferase; PIMT [H]

Number of amino acids: Translated: 209; Mature: 209

Protein sequence:

>209_residues
MKSIKNHLMCEEINKRFSLHPKVREAMESIEREVFVPAPFKHFAYTLNALSMQAQQYISSPLTVAKMTQYLEIDHVDSVL
EIGCGSGYQAAVLSQIFRRVFSIERIESLYIEARLRLKTLGLNNVHVKFADGNKGWDQYAPYDRILFSACAKNIPQALID
QLEEGGILVAPIQENNEQVIKRFVKQNNALRVQKVLEKCLFVPVVDGVQ

Sequences:

>Translated_209_residues
MKSIKNHLMCEEINKRFSLHPKVREAMESIEREVFVPAPFKHFAYTLNALSMQAQQYISSPLTVAKMTQYLEIDHVDSVL
EIGCGSGYQAAVLSQIFRRVFSIERIESLYIEARLRLKTLGLNNVHVKFADGNKGWDQYAPYDRILFSACAKNIPQALID
QLEEGGILVAPIQENNEQVIKRFVKQNNALRVQKVLEKCLFVPVVDGVQ
>Mature_209_residues
MKSIKNHLMCEEINKRFSLHPKVREAMESIEREVFVPAPFKHFAYTLNALSMQAQQYISSPLTVAKMTQYLEIDHVDSVL
EIGCGSGYQAAVLSQIFRRVFSIERIESLYIEARLRLKTLGLNNVHVKFADGNKGWDQYAPYDRILFSACAKNIPQALID
QLEEGGILVAPIQENNEQVIKRFVKQNNALRVQKVLEKCLFVPVVDGVQ

Specific function: Catalyzes the methyl esterification of L-isoaspartyl residues in peptides and proteins that result from spontaneous decomposition of normal L-aspartyl and L-asparaginyl residues. It plays a role in the repair and/or degradation of damaged proteins [H]

COG id: COG2518

COG function: function code O; Protein-L-isoaspartate carboxylmethyltransferase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. L- isoaspartyl/D-aspartyl protein methyltransferase family [H]

Homologues:

Organism=Homo sapiens, GI226530908, Length=189, Percent_Identity=28.042328042328, Blast_Score=67, Evalue=1e-11,
Organism=Escherichia coli, GI1789100, Length=186, Percent_Identity=43.010752688172, Blast_Score=145, Evalue=2e-36,
Organism=Caenorhabditis elegans, GI193207222, Length=205, Percent_Identity=29.2682926829268, Blast_Score=78, Evalue=4e-15,
Organism=Caenorhabditis elegans, GI71983477, Length=205, Percent_Identity=28.2926829268293, Blast_Score=77, Evalue=4e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000682 [H]

Pfam domain/function: PF01135 PCMT [H]

EC number: =2.1.1.77 [H]

Molecular weight: Translated: 23840; Mature: 23840

Theoretical pI: Translated: 8.31; Mature: 8.31

Prosite motif: PS01279 PCMT

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKSIKNHLMCEEINKRFSLHPKVREAMESIEREVFVPAPFKHFAYTLNALSMQAQQYISS
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCEECCCCHHHHHHHHHHHHHHHHHHHHC
PLTVAKMTQYLEIDHVDSVLEIGCGSGYQAAVLSQIFRRVFSIERIESLYIEARLRLKTL
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
GLNNVHVKFADGNKGWDQYAPYDRILFSACAKNIPQALIDQLEEGGILVAPIQENNEQVI
CCCCEEEEECCCCCCCHHCCCHHHHHHHHHHHHCHHHHHHHHHCCCEEEEECCCCCHHHH
KRFVKQNNALRVQKVLEKCLFVPVVDGVQ
HHHHHCCCCHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKSIKNHLMCEEINKRFSLHPKVREAMESIEREVFVPAPFKHFAYTLNALSMQAQQYISS
CCCHHHHHHHHHHHHHCCCCHHHHHHHHHHHHCEECCCCHHHHHHHHHHHHHHHHHHHHC
PLTVAKMTQYLEIDHVDSVLEIGCGSGYQAAVLSQIFRRVFSIERIESLYIEARLRLKTL
CHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
GLNNVHVKFADGNKGWDQYAPYDRILFSACAKNIPQALIDQLEEGGILVAPIQENNEQVI
CCCCEEEEECCCCCCCHHCCCHHHHHHHHHHHHCHHHHHHHHHCCCEEEEECCCCCHHHH
KRFVKQNNALRVQKVLEKCLFVPVVDGVQ
HHHHHCCCCHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA