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The map label for this gene is nrdF

Identifier: 188527870

GI number: 188527870

Start: 1104330

End: 1105355

Strand: Direct

Name: nrdF

Synonym: HPSH_05595

Alternate gene names: 188527870

Gene position: 1104330-1105355 (Clockwise)

Preceding gene: 188527869

Following gene: 188527871

Centisome position: 68.65

GC content: 39.67

Gene sequence:

>1026_bases
ATGGAAGTTTCACGCAAGAAAATTTACAACCCCAATTCTACAGAAAGTGTGAATGAAAGAAAGATTTTTGGGGGCAATCC
TACGAGCATGTTTGATTTGAATAAGATCAAGTATCAATGGGCGGATCATTTGTGGAAAACGATGCTCGCTAACACCTGGT
TTGCTGAAGAAGTGAGCATGAATGATGACAAAAGGGATTATTTGAAATTAAGCGCAGAAGAAAAGATCGGCTATGACAGA
GCTTTAGCGCAACTCATTTTTATGGATAGCTTGCAAACCAATAATTTAATTGATAATGTCAATCCTTTTATCACCAGCCC
CGAAATCAATTTGTGTTTGGTGCGTCAAGCTTATGAAGAAGCCTTACACAGCCATGCGTATGCGGTGATGGTAGAAAGCA
TAAGCGCCAATACTGAAGAGATTTATGACATGTGGCGTAACGACATGCAATTAAAAAGCAAGAACGACTATATCGCGCAA
GTGTATATGGAATTAGCCAAAAACCCTACAGAAGAAAGCATTCTCAAAGCGCTTTTTGCGAACCAGATTTTAGAGGGGAT
TTATTTTTATAGCGGGTTTAGCTATTTTTACACTTTGGCTAGGAGCGGTAAGATGCTAGGATCAGCGCAAATGATTCGTT
TTATCCAAAGAGATGAGGTAACGCATTTGATTTTATTCCAAAACATGATCAACGCTTTAAGGAATGAAAGAGCGGATCTC
TTCACGCCGCAATTGATTAATGAAGTCATAGGAATGTTTAAAAAAGCGGTAGAAATTGAAGCTTCATGGGGGGATTATAT
CACGCAAGGCAAGATTTTAGGGCTCACTTCAAGCTTGATTGAGCAATACATCCAGTTTTTAGCGGATAGCCGTTTGAGTA
AGGTGGGTATCGCTAAGGTTTATGGCGTCCAACACCCCATTAAATGGGTAGAGAGCTTTTCAAGTTTCAATGAGCAGCGC
TCTAATTTCTTTGAAGCTAGGGTGAGTAATTACGCTAAAGGGAGCGTGAGTTTTGACGATTTTTAA

Upstream 100 bases:

>100_bases
GAATTTGAAGTTTTTTCTGGCTTTAGGGAGTTTTAAATTCTTTTAAGGTATTCTAACAAGGCTATATCATTAGATAGTTT
TAAGGAAATTAAGGAACAAA

Downstream 100 bases:

>100_bases
GGGGTTTGTTTGAAGAGTATTAAAAACCATTTGATGTGTGAAGAAATCAATAAGCGTTTCAGTTTGCACCCCAAAGTGAG
AGAAGCCATGGAAAGCATTG

Product: ribonucleotide-diphosphate reductase subunit beta

Products: NA

Alternate protein names: Ribonucleotide reductase small subunit [H]

Number of amino acids: Translated: 341; Mature: 341

Protein sequence:

>341_residues
MEVSRKKIYNPNSTESVNERKIFGGNPTSMFDLNKIKYQWADHLWKTMLANTWFAEEVSMNDDKRDYLKLSAEEKIGYDR
ALAQLIFMDSLQTNNLIDNVNPFITSPEINLCLVRQAYEEALHSHAYAVMVESISANTEEIYDMWRNDMQLKSKNDYIAQ
VYMELAKNPTEESILKALFANQILEGIYFYSGFSYFYTLARSGKMLGSAQMIRFIQRDEVTHLILFQNMINALRNERADL
FTPQLINEVIGMFKKAVEIEASWGDYITQGKILGLTSSLIEQYIQFLADSRLSKVGIAKVYGVQHPIKWVESFSSFNEQR
SNFFEARVSNYAKGSVSFDDF

Sequences:

>Translated_341_residues
MEVSRKKIYNPNSTESVNERKIFGGNPTSMFDLNKIKYQWADHLWKTMLANTWFAEEVSMNDDKRDYLKLSAEEKIGYDR
ALAQLIFMDSLQTNNLIDNVNPFITSPEINLCLVRQAYEEALHSHAYAVMVESISANTEEIYDMWRNDMQLKSKNDYIAQ
VYMELAKNPTEESILKALFANQILEGIYFYSGFSYFYTLARSGKMLGSAQMIRFIQRDEVTHLILFQNMINALRNERADL
FTPQLINEVIGMFKKAVEIEASWGDYITQGKILGLTSSLIEQYIQFLADSRLSKVGIAKVYGVQHPIKWVESFSSFNEQR
SNFFEARVSNYAKGSVSFDDF
>Mature_341_residues
MEVSRKKIYNPNSTESVNERKIFGGNPTSMFDLNKIKYQWADHLWKTMLANTWFAEEVSMNDDKRDYLKLSAEEKIGYDR
ALAQLIFMDSLQTNNLIDNVNPFITSPEINLCLVRQAYEEALHSHAYAVMVESISANTEEIYDMWRNDMQLKSKNDYIAQ
VYMELAKNPTEESILKALFANQILEGIYFYSGFSYFYTLARSGKMLGSAQMIRFIQRDEVTHLILFQNMINALRNERADL
FTPQLINEVIGMFKKAVEIEASWGDYITQGKILGLTSSLIEQYIQFLADSRLSKVGIAKVYGVQHPIKWVESFSSFNEQR
SNFFEARVSNYAKGSVSFDDF

Specific function: Provides the precursors necessary for DNA synthesis. Catalyzes the biosynthesis of deoxyribonucleotides from the corresponding ribonucleotides [H]

COG id: COG0208

COG function: function code F; Ribonucleotide reductase, beta subunit

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the ribonucleoside diphosphate reductase small chain family [H]

Homologues:

Organism=Homo sapiens, GI42544136, Length=313, Percent_Identity=27.1565495207668, Blast_Score=100, Evalue=3e-21,
Organism=Homo sapiens, GI289177074, Length=313, Percent_Identity=27.1565495207668, Blast_Score=99, Evalue=5e-21,
Organism=Homo sapiens, GI4557845, Length=331, Percent_Identity=24.773413897281, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI260064013, Length=331, Percent_Identity=24.773413897281, Blast_Score=97, Evalue=2e-20,
Organism=Homo sapiens, GI289177076, Length=288, Percent_Identity=26.7361111111111, Blast_Score=91, Evalue=1e-18,
Organism=Escherichia coli, GI1788567, Length=345, Percent_Identity=25.2173913043478, Blast_Score=116, Evalue=2e-27,
Organism=Escherichia coli, GI1789031, Length=196, Percent_Identity=26.0204081632653, Blast_Score=65, Evalue=5e-12,
Organism=Caenorhabditis elegans, GI17551968, Length=297, Percent_Identity=26.9360269360269, Blast_Score=93, Evalue=2e-19,
Organism=Saccharomyces cerevisiae, GI6322434, Length=310, Percent_Identity=25.8064516129032, Blast_Score=93, Evalue=6e-20,
Organism=Saccharomyces cerevisiae, GI6321619, Length=309, Percent_Identity=26.8608414239482, Blast_Score=79, Evalue=1e-15,
Organism=Drosophila melanogaster, GI18079279, Length=318, Percent_Identity=25.1572327044025, Blast_Score=96, Evalue=3e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009078
- InterPro:   IPR012348
- InterPro:   IPR000358 [H]

Pfam domain/function: PF00268 Ribonuc_red_sm [H]

EC number: =1.17.4.1 [H]

Molecular weight: Translated: 39445; Mature: 39445

Theoretical pI: Translated: 5.13; Mature: 5.13

Prosite motif: PS00368 RIBORED_SMALL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEVSRKKIYNPNSTESVNERKIFGGNPTSMFDLNKIKYQWADHLWKTMLANTWFAEEVSM
CCCCCCCCCCCCCCCCCCCCEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
NDDKRDYLKLSAEEKIGYDRALAQLIFMDSLQTNNLIDNVNPFITSPEINLCLVRQAYEE
CCCCHHHHEECCHHHCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHH
ALHSHAYAVMVESISANTEEIYDMWRNDMQLKSKNDYIAQVYMELAKNPTEESILKALFA
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
NQILEGIYFYSGFSYFYTLARSGKMLGSAQMIRFIQRDEVTHLILFQNMINALRNERADL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHC
FTPQLINEVIGMFKKAVEIEASWGDYITQGKILGLTSSLIEQYIQFLADSRLSKVGIAKV
CCHHHHHHHHHHHHHHHHEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGVQHPIKWVESFSSFNEQRSNFFEARVSNYAKGSVSFDDF
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure
MEVSRKKIYNPNSTESVNERKIFGGNPTSMFDLNKIKYQWADHLWKTMLANTWFAEEVSM
CCCCCCCCCCCCCCCCCCCCEEECCCCCCEEHHHHHHHHHHHHHHHHHHHCCHHHHHCCC
NDDKRDYLKLSAEEKIGYDRALAQLIFMDSLQTNNLIDNVNPFITSPEINLCLVRQAYEE
CCCCHHHHEECCHHHCCHHHHHHHHHHHHHHCCCCHHHCCCCCCCCCCCCHHHHHHHHHH
ALHSHAYAVMVESISANTEEIYDMWRNDMQLKSKNDYIAQVYMELAKNPTEESILKALFA
HHHHHHHHHHHHHHCCCHHHHHHHHHCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHH
NQILEGIYFYSGFSYFYTLARSGKMLGSAQMIRFIQRDEVTHLILFQNMINALRNERADL
HHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHC
FTPQLINEVIGMFKKAVEIEASWGDYITQGKILGLTSSLIEQYIQFLADSRLSKVGIAKV
CCHHHHHHHHHHHHHHHHEECCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
YGVQHPIKWVESFSSFNEQRSNFFEARVSNYAKGSVSFDDF
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]