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The map label for this gene is pheS

Identifier: 188527832

GI number: 188527832

Start: 1062099

End: 1063085

Strand: Direct

Name: pheS

Synonym: HPSH_05390

Alternate gene names: 188527832

Gene position: 1062099-1063085 (Clockwise)

Preceding gene: 188527821

Following gene: 188527833

Centisome position: 66.03

GC content: 41.03

Gene sequence:

>987_bases
TTGCACACCTTAATAGAGCGATTAGAAAAGGTTACTAATAGCAAAGAGTTAGAAGAAGCGCGCTTGAGTGCTTTGGGTAA
AAAAGGGGTTTTTGCGGATAAATTCAACCAGCTCAAAAATCTAAACGGCGAAGAAAAAAACGCCTTTGCTAAAGAAATCC
ACCATTATAAACAAGCGTTTGAAAAGGCCTTTGAGTTGAAAAAAAAGGCGATTTTAGAGCTTGAATTAGAAGAACGCTTG
AAAAAAGAAAAAATTGATGTGAGTTTGTTTAACGCTATTAAAACAAGCTCTTCTCACCCTTTAAATCACACTAAAAATAA
AATCATTGAATTTTTTACCCCATTAGGATACAAGCTTGAAATCGGCTCTTTGGTGGAAGATGATTTCCATAATTTCAGCG
CTTTAAACTTGCCCCCCTACCATCCTGCAAGGGACATGCAAGACACTTTCTATTTTAAAGATCACAAGCTTTTAAGGACC
CACACTTCGCCCGTTCAAATCCACACCATGCAAGAACAAACCCCACCCATTAAGATGATTTGTTTAGGCGAAACCTTTAG
GCGCGATTATGACTTGACCCACACGCCCATGTTCCATCAAATTGAAGGGCTTGTCGTGGATCAAAAAGGGAATATCCGTT
TCACGCACTTAAAAGGCGTGATTGAAGACTTTTTGCATTATTTCTTTGGCGGCGTGAAATTAAGGTGGCGCTCTAGCTTT
TTCCCTTTCACAGAGCCAAGCGCTGAAGTGGATATTAGTTGCGTGTTTTGCAAGCAAGAAGGCTGTAGGGTTTGCTCGCA
CACAGGCTGGCTAGAAGTGTTAGGCTGTGGCATGGTCAATAATGCGGTGTTTGAAGCCATAGGGTATGAGAATGTGAGCG
GGTTTGCTTTTGGCATGGGGATTGAAAGGTTAGCCATGCTGACTTGCCAGATCAATGATTTGCGCAGTTTCTTTGAAACT
GATTTGAGAGTGTTGGAGAGTTTTTAA

Upstream 100 bases:

>100_bases
ATTTTTTCAAACACATTCATGCTTTTTTGCTCCCTTTTAAGGTTTTTCTTTTATTAAAGTTGTATTATAGCTTTTTAAAA
ATAAAATATAAGGATCGTTA

Downstream 100 bases:

>100_bases
TGAAACTGAGTGTTAATGATTTGAGCGTTTTTGTAGATACGCCTAAAGATATAGCCAAACTCTGTGAAGATTTGAGCCGC
TTAGGTTTAGAAGTGGAGAG

Product: phenylalanyl-tRNA synthetase subunit alpha

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase alpha chain; PheRS [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MHTLIERLEKVTNSKELEEARLSALGKKGVFADKFNQLKNLNGEEKNAFAKEIHHYKQAFEKAFELKKKAILELELEERL
KKEKIDVSLFNAIKTSSSHPLNHTKNKIIEFFTPLGYKLEIGSLVEDDFHNFSALNLPPYHPARDMQDTFYFKDHKLLRT
HTSPVQIHTMQEQTPPIKMICLGETFRRDYDLTHTPMFHQIEGLVVDQKGNIRFTHLKGVIEDFLHYFFGGVKLRWRSSF
FPFTEPSAEVDISCVFCKQEGCRVCSHTGWLEVLGCGMVNNAVFEAIGYENVSGFAFGMGIERLAMLTCQINDLRSFFET
DLRVLESF

Sequences:

>Translated_328_residues
MHTLIERLEKVTNSKELEEARLSALGKKGVFADKFNQLKNLNGEEKNAFAKEIHHYKQAFEKAFELKKKAILELELEERL
KKEKIDVSLFNAIKTSSSHPLNHTKNKIIEFFTPLGYKLEIGSLVEDDFHNFSALNLPPYHPARDMQDTFYFKDHKLLRT
HTSPVQIHTMQEQTPPIKMICLGETFRRDYDLTHTPMFHQIEGLVVDQKGNIRFTHLKGVIEDFLHYFFGGVKLRWRSSF
FPFTEPSAEVDISCVFCKQEGCRVCSHTGWLEVLGCGMVNNAVFEAIGYENVSGFAFGMGIERLAMLTCQINDLRSFFET
DLRVLESF
>Mature_328_residues
MHTLIERLEKVTNSKELEEARLSALGKKGVFADKFNQLKNLNGEEKNAFAKEIHHYKQAFEKAFELKKKAILELELEERL
KKEKIDVSLFNAIKTSSSHPLNHTKNKIIEFFTPLGYKLEIGSLVEDDFHNFSALNLPPYHPARDMQDTFYFKDHKLLRT
HTSPVQIHTMQEQTPPIKMICLGETFRRDYDLTHTPMFHQIEGLVVDQKGNIRFTHLKGVIEDFLHYFFGGVKLRWRSSF
FPFTEPSAEVDISCVFCKQEGCRVCSHTGWLEVLGCGMVNNAVFEAIGYENVSGFAFGMGIERLAMLTCQINDLRSFFET
DLRVLESF

Specific function: Unknown

COG id: COG0016

COG function: function code J; Phenylalanyl-tRNA synthetase alpha subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-II aminoacyl-tRNA synthetase family. Phe-tRNA synthetase alpha chain type 1 subfamily [H]

Homologues:

Organism=Homo sapiens, GI5729820, Length=274, Percent_Identity=29.9270072992701, Blast_Score=114, Evalue=9e-26,
Organism=Homo sapiens, GI4758340, Length=264, Percent_Identity=29.1666666666667, Blast_Score=106, Evalue=4e-23,
Organism=Escherichia coli, GI1788007, Length=325, Percent_Identity=46.4615384615385, Blast_Score=284, Evalue=6e-78,
Organism=Caenorhabditis elegans, GI32566635, Length=287, Percent_Identity=29.616724738676, Blast_Score=114, Evalue=5e-26,
Organism=Caenorhabditis elegans, GI17508957, Length=277, Percent_Identity=28.158844765343, Blast_Score=102, Evalue=2e-22,
Organism=Caenorhabditis elegans, GI32563657, Length=277, Percent_Identity=28.158844765343, Blast_Score=102, Evalue=2e-22,
Organism=Saccharomyces cerevisiae, GI6321087, Length=272, Percent_Identity=27.2058823529412, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6325304, Length=313, Percent_Identity=26.8370607028754, Blast_Score=91, Evalue=2e-19,
Organism=Drosophila melanogaster, GI17137424, Length=289, Percent_Identity=27.681660899654, Blast_Score=101, Evalue=7e-22,
Organism=Drosophila melanogaster, GI18858079, Length=278, Percent_Identity=27.3381294964029, Blast_Score=92, Evalue=6e-19,

Paralogues:

None

Copy number: 2,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006195
- InterPro:   IPR004529
- InterPro:   IPR004188
- InterPro:   IPR022911
- InterPro:   IPR002319
- InterPro:   IPR010978 [H]

Pfam domain/function: PF02912 Phe_tRNA-synt_N; PF01409 tRNA-synt_2d [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 37896; Mature: 37896

Theoretical pI: Translated: 6.92; Mature: 6.92

Prosite motif: PS50862 AA_TRNA_LIGASE_II

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
2.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MHTLIERLEKVTNSKELEEARLSALGKKGVFADKFNQLKNLNGEEKNAFAKEIHHYKQAF
CCHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EKAFELKKKAILELELEERLKKEKIDVSLFNAIKTSSSHPLNHTKNKIIEFFTPLGYKLE
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEE
IGSLVEDDFHNFSALNLPPYHPARDMQDTFYFKDHKLLRTHTSPVQIHTMQEQTPPIKMI
ECCHHHHHHCCCEECCCCCCCCCCCCCHHHEECCCHHEEECCCCEEEEEECCCCCCEEEE
CLGETFRRDYDLTHTPMFHQIEGLVVDQKGNIRFTHLKGVIEDFLHYFFGGVKLRWRSSF
ECCHHHHCCCCCCCCCHHHHHCCEEEECCCCEEEHHHHHHHHHHHHHHHCCCEEEEECCC
FPFTEPSAEVDISCVFCKQEGCRVCSHTGWLEVLGCGMVNNAVFEAIGYENVSGFAFGMG
CCCCCCCCCEEEEEEEECCCCCCHHCCCCHHHHHHCCHHHHHHHHHHCCCCCCCEEEHHH
IERLAMLTCQINDLRSFFETDLRVLESF
HHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MHTLIERLEKVTNSKELEEARLSALGKKGVFADKFNQLKNLNGEEKNAFAKEIHHYKQAF
CCHHHHHHHHHHCHHHHHHHHHHHHCCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHH
EKAFELKKKAILELELEERLKKEKIDVSLFNAIKTSSSHPLNHTKNKIIEFFTPLGYKLE
HHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHCCCCCCCCHHHHHHHHHHCCCCCEEE
IGSLVEDDFHNFSALNLPPYHPARDMQDTFYFKDHKLLRTHTSPVQIHTMQEQTPPIKMI
ECCHHHHHHCCCEECCCCCCCCCCCCCHHHEECCCHHEEECCCCEEEEEECCCCCCEEEE
CLGETFRRDYDLTHTPMFHQIEGLVVDQKGNIRFTHLKGVIEDFLHYFFGGVKLRWRSSF
ECCHHHHCCCCCCCCCHHHHHCCEEEECCCCEEEHHHHHHHHHHHHHHHCCCEEEEECCC
FPFTEPSAEVDISCVFCKQEGCRVCSHTGWLEVLGCGMVNNAVFEAIGYENVSGFAFGMG
CCCCCCCCCEEEEEEEECCCCCCHHCCCCHHHHHHCCHHHHHHHHHHCCCCCCCEEEHHH
IERLAMLTCQINDLRSFFETDLRVLESF
HHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA