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The map label for this gene is pheT

Identifier: 188527833

GI number: 188527833

Start: 1063085

End: 1065379

Strand: Direct

Name: pheT

Synonym: HPSH_05395

Alternate gene names: 188527833

Gene position: 1063085-1065379 (Clockwise)

Preceding gene: 188527832

Following gene: 188527834

Centisome position: 66.09

GC content: 39.78

Gene sequence:

>2295_bases
ATGAAACTGAGTGTTAATGATTTGAGCGTTTTTGTAGATACGCCTAAAGATATAGCCAAACTCTGTGAAGATTTGAGCCG
CTTAGGTTTAGAAGTGGAGAGCTGTATCCCTTGTGTTGCTCCTAAAAGTGTGGTTGTTGGTAAGGTTTTAGAAAAAGCCC
CCCATAAAAACGCTGAAAAACTCAGCGTGTGTCAAGTGGGTGTGGGTAAGGAAGTGTTACAAATCGTGTGTGGGGCTAAA
AATGTCGCGCCAAACCAATTCGTGCCAGTCGCTTTAAATGGGGCGCTAATCGGCTCAACGACCATCGCTAAAACTGAGCT
TAGGGGGATTGAAAGCTGTGGCATGATTTGCTCTAGCAATGAATTGGGATTCCCTAAAATCAATGATGGCATCTTGGAAT
TAGATGAGAGCGTTGGGGAGTTGGTTTTAGGGAAAGGGTTAAACGAATACGCCCCTTTCAACACGCATGTTTTAGAAATT
TCATTGACTCCTAATCGTGGGGATTGCTTGAGCGTTTTAGGGATTGCTAGAGAGGTTAGCGCGTTTTATCACACGCCCCT
AAAGCCTATTAAGGCTTTAAATTTTACGCCAAAAAGCGATTTGATCACGCTTCATGCGGGTGAAAATATTGAATCGCATC
TGGCTTATTATTTGATTTGCAATCATTCTTTAAAAACCCCTTTAAGCGTCAAACTTTCGCTCGCTCATAATAATGCCTTG
AGCGAGAACGACCTAAACAATTTCATAGAATTTAGCACGCATTTTAGTGGGGTGGTGATGAACGCTTATAGCTTGGATGC
AACCCCTATTGATTTGAGCGTAAAAAACGATGAAAACAACCTTGAAAGCGTTTATATCAACCATCAAAAACGCTCCACTA
TCGCTATCAAATATCAAGATCAAAAAGATTTGAGCGAGTGTTTGCTTTTAGAAGCGAGCTATACTGATCCTATAAGCCTG
TCTTTAAAATTGCACGCCCTAAAAGATAAAACGCTTCAAAAAGACAACGCCCTTATTTATAGAAGCGCTAGGGGGAGCAA
CCCTAATTTATCAGACGGCTTGAATTTTTTAAGCGCCCATTTGAAAGCGGCAATTTTAGAAAGCAAACAAACTAAGCATG
CCTTAAAAGATCGCACCCTTAAATTCAAACTTGAAGATATTACCGAAATTTTGGGGCTTGCCATAGAAGAAGAAAAAATT
CAAGGCATTTTAAAAAATTTAGGCTTTAAAGTTAGCGCAAAAGTGCCAAACTCAAAACCCCAAATTTTAGAGGTTGTTGC
GCCGAATTTCAGGCATGACATTAAAACGATCCAAGATATTGCTGAAGAAATCTTACGCTTTGTAGGGATTGATCATTTGA
TTTCAAAGCCCCTTGATAGCGTCAGTAACAAAAAATCAAACCCCCATTACGACACGCACCGCTTTTTTGAAAACCTTAAA
CACAAGGCTCTCGCTTGCGGTTTTAAAGAAGTCATTCATTATGTGTTTTACTCTAAAGAAAAGCAGCAAAAACTAGGCTT
TGAAGTTTTAGAAGATCCCCTAGAATTGCAAAACCCTATCACAACGGAGTTAAACACCCTAAGAACGAGCCTTGTTTGCG
GGCTTTTAGACGCCAGTTTAAGGAATAAAAATTTAGGGTTTAAAAGCATAGCCCTTTATGAAAAAGGGAGCGTGTATAAC
TCTAAAAGAGAAGAGATCCAAAAACTAGGCTTTTTAGCGAGCGGCTTACAAAAAAAAGAAAGCTACCCTGATGCTAAAGG
CAAGGCTTGGGATTTTTATTCTTTTGCCGAATGCGTTTCAAAAGTTATAGGGGATTTTAGCTTGGAAAAACTGACCACTC
AAGCCCCCATTAACCACCCCTACCAGAGCGCTAAAATCATTCAAAATCATGAAATCATAGGCGTGATTGCTAAAATCCAC
CCCAAAGTGATTCAGGAATTGGATTTGTTTGAAAGCTATTACGCTGAGATAGACGCCTCTAAACTCAAACGCCCTGCTAT
GCTATTAAAACCCTTTAGCATTTATCCTAGCAGTGTGAGGGATTTGACTCTAATCATTGATGAGAATACCGCTTTTAGCG
GGATTAAAAAAGCCCTAAAAGACGCTCAAATCCCTAATTTAAGCGAGATTCTACCCCTTGATATTTTTAAAGAAAGCGAT
AATACCATAGCCTTAAGCGTGCGTTGCGTGATTCATTCTTTAGAAAAAACCCTAAATGACGAAGAGGTCAATTCAGCCGT
GCAAAAAGCGCTTGAAATTTTAGAAAAAGAATTTAACGCCCGCCTTAAGGGATAA

Upstream 100 bases:

>100_bases
CTTTTGGCATGGGGATTGAAAGGTTAGCCATGCTGACTTGCCAGATCAATGATTTGCGCAGTTTCTTTGAAACTGATTTG
AGAGTGTTGGAGAGTTTTTA

Downstream 100 bases:

>100_bases
TATAAAGGATAAAATGTGATAGAGCTTGACATTAACGCTAGCGATAAATCGCTCTCACACAGAGCCGTTATTTTTAGCCT
GCTCGCTCAAAAGCCTTGTG

Product: phenylalanyl-tRNA synthetase subunit beta

Products: NA

Alternate protein names: Phenylalanine--tRNA ligase beta chain; PheRS [H]

Number of amino acids: Translated: 764; Mature: 764

Protein sequence:

>764_residues
MKLSVNDLSVFVDTPKDIAKLCEDLSRLGLEVESCIPCVAPKSVVVGKVLEKAPHKNAEKLSVCQVGVGKEVLQIVCGAK
NVAPNQFVPVALNGALIGSTTIAKTELRGIESCGMICSSNELGFPKINDGILELDESVGELVLGKGLNEYAPFNTHVLEI
SLTPNRGDCLSVLGIAREVSAFYHTPLKPIKALNFTPKSDLITLHAGENIESHLAYYLICNHSLKTPLSVKLSLAHNNAL
SENDLNNFIEFSTHFSGVVMNAYSLDATPIDLSVKNDENNLESVYINHQKRSTIAIKYQDQKDLSECLLLEASYTDPISL
SLKLHALKDKTLQKDNALIYRSARGSNPNLSDGLNFLSAHLKAAILESKQTKHALKDRTLKFKLEDITEILGLAIEEEKI
QGILKNLGFKVSAKVPNSKPQILEVVAPNFRHDIKTIQDIAEEILRFVGIDHLISKPLDSVSNKKSNPHYDTHRFFENLK
HKALACGFKEVIHYVFYSKEKQQKLGFEVLEDPLELQNPITTELNTLRTSLVCGLLDASLRNKNLGFKSIALYEKGSVYN
SKREEIQKLGFLASGLQKKESYPDAKGKAWDFYSFAECVSKVIGDFSLEKLTTQAPINHPYQSAKIIQNHEIIGVIAKIH
PKVIQELDLFESYYAEIDASKLKRPAMLLKPFSIYPSSVRDLTLIIDENTAFSGIKKALKDAQIPNLSEILPLDIFKESD
NTIALSVRCVIHSLEKTLNDEEVNSAVQKALEILEKEFNARLKG

Sequences:

>Translated_764_residues
MKLSVNDLSVFVDTPKDIAKLCEDLSRLGLEVESCIPCVAPKSVVVGKVLEKAPHKNAEKLSVCQVGVGKEVLQIVCGAK
NVAPNQFVPVALNGALIGSTTIAKTELRGIESCGMICSSNELGFPKINDGILELDESVGELVLGKGLNEYAPFNTHVLEI
SLTPNRGDCLSVLGIAREVSAFYHTPLKPIKALNFTPKSDLITLHAGENIESHLAYYLICNHSLKTPLSVKLSLAHNNAL
SENDLNNFIEFSTHFSGVVMNAYSLDATPIDLSVKNDENNLESVYINHQKRSTIAIKYQDQKDLSECLLLEASYTDPISL
SLKLHALKDKTLQKDNALIYRSARGSNPNLSDGLNFLSAHLKAAILESKQTKHALKDRTLKFKLEDITEILGLAIEEEKI
QGILKNLGFKVSAKVPNSKPQILEVVAPNFRHDIKTIQDIAEEILRFVGIDHLISKPLDSVSNKKSNPHYDTHRFFENLK
HKALACGFKEVIHYVFYSKEKQQKLGFEVLEDPLELQNPITTELNTLRTSLVCGLLDASLRNKNLGFKSIALYEKGSVYN
SKREEIQKLGFLASGLQKKESYPDAKGKAWDFYSFAECVSKVIGDFSLEKLTTQAPINHPYQSAKIIQNHEIIGVIAKIH
PKVIQELDLFESYYAEIDASKLKRPAMLLKPFSIYPSSVRDLTLIIDENTAFSGIKKALKDAQIPNLSEILPLDIFKESD
NTIALSVRCVIHSLEKTLNDEEVNSAVQKALEILEKEFNARLKG
>Mature_764_residues
MKLSVNDLSVFVDTPKDIAKLCEDLSRLGLEVESCIPCVAPKSVVVGKVLEKAPHKNAEKLSVCQVGVGKEVLQIVCGAK
NVAPNQFVPVALNGALIGSTTIAKTELRGIESCGMICSSNELGFPKINDGILELDESVGELVLGKGLNEYAPFNTHVLEI
SLTPNRGDCLSVLGIAREVSAFYHTPLKPIKALNFTPKSDLITLHAGENIESHLAYYLICNHSLKTPLSVKLSLAHNNAL
SENDLNNFIEFSTHFSGVVMNAYSLDATPIDLSVKNDENNLESVYINHQKRSTIAIKYQDQKDLSECLLLEASYTDPISL
SLKLHALKDKTLQKDNALIYRSARGSNPNLSDGLNFLSAHLKAAILESKQTKHALKDRTLKFKLEDITEILGLAIEEEKI
QGILKNLGFKVSAKVPNSKPQILEVVAPNFRHDIKTIQDIAEEILRFVGIDHLISKPLDSVSNKKSNPHYDTHRFFENLK
HKALACGFKEVIHYVFYSKEKQQKLGFEVLEDPLELQNPITTELNTLRTSLVCGLLDASLRNKNLGFKSIALYEKGSVYN
SKREEIQKLGFLASGLQKKESYPDAKGKAWDFYSFAECVSKVIGDFSLEKLTTQAPINHPYQSAKIIQNHEIIGVIAKIH
PKVIQELDLFESYYAEIDASKLKRPAMLLKPFSIYPSSVRDLTLIIDENTAFSGIKKALKDAQIPNLSEILPLDIFKESD
NTIALSVRCVIHSLEKTLNDEEVNSAVQKALEILEKEFNARLKG

Specific function: Unknown

COG id: COG0072

COG function: function code J; Phenylalanyl-tRNA synthetase beta subunit

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 tRNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1788006, Length=815, Percent_Identity=26.8711656441718, Blast_Score=205, Evalue=8e-54,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005147
- InterPro:   IPR009061
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR004532
- InterPro:   IPR005121
- InterPro:   IPR002547 [H]

Pfam domain/function: PF03484 B5; PF03147 FDX-ACB; PF01588 tRNA_bind [H]

EC number: =6.1.1.20 [H]

Molecular weight: Translated: 84766; Mature: 84766

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50886 TRBD

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
0.5 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKLSVNDLSVFVDTPKDIAKLCEDLSRLGLEVESCIPCVAPKSVVVGKVLEKAPHKNAEK
CEEECCCEEEEEECCHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCH
LSVCQVGVGKEVLQIVCGAKNVAPNQFVPVALNGALIGSTTIAKTELRGIESCGMICSSN
HHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCEEECCCHHHHHHHHCHHHHCCEECCC
ELGFPKINDGILELDESVGELVLGKGLNEYAPFNTHVLEISLTPNRGDCLSVLGIAREVS
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHH
AFYHTPLKPIKALNFTPKSDLITLHAGENIESHLAYYLICNHSLKTPLSVKLSLAHNNAL
HHHCCCCCHHHHCCCCCCCCEEEEECCCCHHHHEEEEEEECCCCCCCCEEEEEEECCCCC
SENDLNNFIEFSTHFSGVVMNAYSLDATPIDLSVKNDENNLESVYINHQKRSTIAIKYQD
CCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCEEEEEECC
QKDLSECLLLEASYTDPISLSLKLHALKDKTLQKDNALIYRSARGSNPNLSDGLNFLSAH
HHHHHHHHEEECCCCCCEEEEEEEEEHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
LKAAILESKQTKHALKDRTLKFKLEDITEILGLAIEEEKIQGILKNLGFKVSAKVPNSKP
HHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCHHHHHHHHHHHHHCCEEEEECCCCCCC
QILEVVAPNFRHDIKTIQDIAEEILRFVGIDHLISKPLDSVSNKKSNPHYDTHRFFENLK
CEEEECCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
HKALACGFKEVIHYVFYSKEKQQKLGFEVLEDPLELQNPITTELNTLRTSLVCGLLDASL
HHHHHHHHHHHHHHHHHCCHHHHHCCHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
RNKNLGFKSIALYEKGSVYNSKREEIQKLGFLASGLQKKESYPDAKGKAWDFYSFAECVS
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
KVIGDFSLEKLTTQAPINHPYQSAKIIQNHEIIGVIAKIHPKVIQELDLFESYYAEIDAS
HHHCCCHHHHHHHCCCCCCCCHHHHHHCCCCCEEEHHHHCHHHHHHHHHHHHHHHHCCHH
KLKRPAMLLKPFSIYPSSVRDLTLIIDENTAFSGIKKALKDAQIPNLSEILPLDIFKESD
HCCCCHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCHHHHCCHHEEECCC
NTIALSVRCVIHSLEKTLNDEEVNSAVQKALEILEKEFNARLKG
CEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC
>Mature Secondary Structure
MKLSVNDLSVFVDTPKDIAKLCEDLSRLGLEVESCIPCVAPKSVVVGKVLEKAPHKNAEK
CEEECCCEEEEEECCHHHHHHHHHHHHHCCCHHHHCCCCCCHHHHHHHHHHHCCCCCCCH
LSVCQVGVGKEVLQIVCGAKNVAPNQFVPVALNGALIGSTTIAKTELRGIESCGMICSSN
HHHHHCCCCHHHHHHHHCCCCCCCCCEEEEEECCEEECCCHHHHHHHHCHHHHCCEECCC
ELGFPKINDGILELDESVGELVLGKGLNEYAPFNTHVLEISLTPNRGDCLSVLGIAREVS
CCCCCCCCCHHHHHHHHHHHHHHCCCCCCCCCCCEEEEEEEECCCCCCHHHHHHHHHHHH
AFYHTPLKPIKALNFTPKSDLITLHAGENIESHLAYYLICNHSLKTPLSVKLSLAHNNAL
HHHCCCCCHHHHCCCCCCCCEEEEECCCCHHHHEEEEEEECCCCCCCCEEEEEEECCCCC
SENDLNNFIEFSTHFSGVVMNAYSLDATPIDLSVKNDENNLESVYINHQKRSTIAIKYQD
CCCHHHHHHHHHHCCCEEEEEEEECCCCCEEEEEECCCCCCEEEEEECCCCCEEEEEECC
QKDLSECLLLEASYTDPISLSLKLHALKDKTLQKDNALIYRSARGSNPNLSDGLNFLSAH
HHHHHHHHEEECCCCCCEEEEEEEEEHHCCCCCCCCEEEEEECCCCCCCHHHHHHHHHHH
LKAAILESKQTKHALKDRTLKFKLEDITEILGLAIEEEKIQGILKNLGFKVSAKVPNSKP
HHHHHHHHHHHHHHHHCCCEEEEHHHHHHHHHCHHHHHHHHHHHHHCCEEEEECCCCCCC
QILEVVAPNFRHDIKTIQDIAEEILRFVGIDHLISKPLDSVSNKKSNPHYDTHRFFENLK
CEEEECCCCHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHH
HKALACGFKEVIHYVFYSKEKQQKLGFEVLEDPLELQNPITTELNTLRTSLVCGLLDASL
HHHHHHHHHHHHHHHHHCCHHHHHCCHHHHCCCHHHCCCCHHHHHHHHHHHHHHHHHHHH
RNKNLGFKSIALYEKGSVYNSKREEIQKLGFLASGLQKKESYPDAKGKAWDFYSFAECVS
CCCCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHH
KVIGDFSLEKLTTQAPINHPYQSAKIIQNHEIIGVIAKIHPKVIQELDLFESYYAEIDAS
HHHCCCHHHHHHHCCCCCCCCHHHHHHCCCCCEEEHHHHCHHHHHHHHHHHHHHHHCCHH
KLKRPAMLLKPFSIYPSSVRDLTLIIDENTAFSGIKKALKDAQIPNLSEILPLDIFKESD
HCCCCHHHCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHCCCCCHHHHCCHHEEECCC
NTIALSVRCVIHSLEKTLNDEEVNSAVQKALEILEKEFNARLKG
CEEEEEHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]