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The map label for this gene is truD

Identifier: 188527749

GI number: 188527749

Start: 970408

End: 971553

Strand: Direct

Name: truD

Synonym: HPSH_04880

Alternate gene names: 188527749

Gene position: 970408-971553 (Clockwise)

Preceding gene: 188527748

Following gene: 188527750

Centisome position: 60.33

GC content: 35.51

Gene sequence:

>1146_bases
ATGAATTTAAATTTTATGCCCCTATTGCATGCTTATAACCATGCGAGCATTGATTTTCATTTCAATTCTAGCGCTAGGGA
TTTTTGCGTGCATGAAGTGCCTTTGTATGAATTCAGTAACACAGGCGAACATGCCGTTATTCAAGTGAGAAAAAGCGGTT
TAAGCACTTTAGAAATGCTTCAAATTTTTTCTCAAATTTTAGGGGTAAGAATCGCTGAATTGGGTTATGCGGGCTTGAAA
GATAAAAACGCGCTGACGACTCAATTCATCTCACTCCCTAAAAAATACGCCCCTTTATTAGAAAAAAATACGAGCAACTT
TCAAGAAAAAAACCTTAAAATTCTGTCTTTGAATTACCACCATAATAAAATCAAATTGGGGCATTTGAAAGGGAATCGCT
TTTTTATGCGTTTTAAAAAAATGACCCCCCTAAACGCTCAAAAGACTGAACAGGTTTTAGAACAAATCGCGCGGTTTGGC
ATGCCTAATTATTTTGGCCCGCAACGCTTTGGGAAGTTCAATGACAACCACCAAGAGGGTTTAAAAATCTTGCAAAATCA
AACGAAATTCGCCCATCAAAAATTAAACGCTTTTTTAATTTCAAGCTATCAAAGTTATTTGTTTAACGCGCTTTTAAGCA
AACGATTAGAAATCAGTAAAATCATTAGTGCTTTTAGTGTCAAAGAAAATTTAGAATTTTTTAAACAAAAAAATTTAAAT
ATCAATCCAAACACCCTAAAAGCCCTTAAAAACCAAGCCCACCCCTTTAAAATCTTAGAAGGCGATGTGATGCGCCATTA
CCCTTATGGGAAGTTTTTTGACGCTTTAGAATTAGAAAAAGAGAGCGAAAGGTTTTTGAAAAAAGAAGCTGCACCTACGG
GGTTACTAGATGGCAAAAAAGCTCTTTATGCAAAAAATTTGAGTTTAGAAATTGAAAAAGAATTCCAGCATAACCTTTTA
AGTAGCCATGCTAAAACGCTAGGCTCTAGGCGGTTTTTTTGGGTGTTTGCAGAAAATGTAACTTCTCAATATGTGAAAGA
AAAAGCGCAATTTGAATTGGGATTTTACTTGCCTAAAGGGAGTTATGCGAGCGCGTTGCTCAAAGAAATCAAGCATGAGA
AAGGAGAAAATAATGACGAATTTTGA

Upstream 100 bases:

>100_bases
TCACTTTCACTAAAATCGCTCAAGGCGTGCCTACTGGAGTGAATTTTGAAAACATTGACTCAGTTTCGCTCTCAAGGGCG
TTTAATTCAAGGATCAAAGC

Downstream 100 bases:

>100_bases
AAAAATTATCGCGCAAAACAAGCTCAAAACAAACGCGGTTTTAGCGACTTATTGCGTGATTTTTGCTTTTATTGGGTTGT
TGGTGGATGTTATTAGAATT

Product: tRNA pseudouridine synthase D

Products: NA

Alternate protein names: tRNA pseudouridylate synthase D; tRNA-uridine isomerase D [H]

Number of amino acids: Translated: 381; Mature: 381

Protein sequence:

>381_residues
MNLNFMPLLHAYNHASIDFHFNSSARDFCVHEVPLYEFSNTGEHAVIQVRKSGLSTLEMLQIFSQILGVRIAELGYAGLK
DKNALTTQFISLPKKYAPLLEKNTSNFQEKNLKILSLNYHHNKIKLGHLKGNRFFMRFKKMTPLNAQKTEQVLEQIARFG
MPNYFGPQRFGKFNDNHQEGLKILQNQTKFAHQKLNAFLISSYQSYLFNALLSKRLEISKIISAFSVKENLEFFKQKNLN
INPNTLKALKNQAHPFKILEGDVMRHYPYGKFFDALELEKESERFLKKEAAPTGLLDGKKALYAKNLSLEIEKEFQHNLL
SSHAKTLGSRRFFWVFAENVTSQYVKEKAQFELGFYLPKGSYASALLKEIKHEKGENNDEF

Sequences:

>Translated_381_residues
MNLNFMPLLHAYNHASIDFHFNSSARDFCVHEVPLYEFSNTGEHAVIQVRKSGLSTLEMLQIFSQILGVRIAELGYAGLK
DKNALTTQFISLPKKYAPLLEKNTSNFQEKNLKILSLNYHHNKIKLGHLKGNRFFMRFKKMTPLNAQKTEQVLEQIARFG
MPNYFGPQRFGKFNDNHQEGLKILQNQTKFAHQKLNAFLISSYQSYLFNALLSKRLEISKIISAFSVKENLEFFKQKNLN
INPNTLKALKNQAHPFKILEGDVMRHYPYGKFFDALELEKESERFLKKEAAPTGLLDGKKALYAKNLSLEIEKEFQHNLL
SSHAKTLGSRRFFWVFAENVTSQYVKEKAQFELGFYLPKGSYASALLKEIKHEKGENNDEF
>Mature_381_residues
MNLNFMPLLHAYNHASIDFHFNSSARDFCVHEVPLYEFSNTGEHAVIQVRKSGLSTLEMLQIFSQILGVRIAELGYAGLK
DKNALTTQFISLPKKYAPLLEKNTSNFQEKNLKILSLNYHHNKIKLGHLKGNRFFMRFKKMTPLNAQKTEQVLEQIARFG
MPNYFGPQRFGKFNDNHQEGLKILQNQTKFAHQKLNAFLISSYQSYLFNALLSKRLEISKIISAFSVKENLEFFKQKNLN
INPNTLKALKNQAHPFKILEGDVMRHYPYGKFFDALELEKESERFLKKEAAPTGLLDGKKALYAKNLSLEIEKEFQHNLL
SSHAKTLGSRRFFWVFAENVTSQYVKEKAQFELGFYLPKGSYASALLKEIKHEKGENNDEF

Specific function: Responsible for synthesis of pseudouridine from uracil- 13 in transfer RNAs [H]

COG id: COG0585

COG function: function code S; Uncharacterized conserved protein

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 TRUD domain [H]

Homologues:

Organism=Homo sapiens, GI148762985, Length=147, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI148762961, Length=147, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI148762948, Length=147, Percent_Identity=33.3333333333333, Blast_Score=74, Evalue=3e-13,
Organism=Homo sapiens, GI50727002, Length=133, Percent_Identity=33.0827067669173, Blast_Score=66, Evalue=6e-11,
Organism=Escherichia coli, GI1789102, Length=361, Percent_Identity=27.1468144044321, Blast_Score=127, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6324817, Length=149, Percent_Identity=30.8724832214765, Blast_Score=64, Evalue=4e-11,
Organism=Drosophila melanogaster, GI21355799, Length=158, Percent_Identity=27.2151898734177, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR001656
- InterPro:   IPR020119
- InterPro:   IPR011760 [H]

Pfam domain/function: PF01142 TruD [H]

EC number: NA

Molecular weight: Translated: 44089; Mature: 44089

Theoretical pI: Translated: 10.11; Mature: 10.11

Prosite motif: PS50984 TRUD ; PS01268 UPF0024

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNLNFMPLLHAYNHASIDFHFNSSARDFCVHEVPLYEFSNTGEHAVIQVRKSGLSTLEML
CCCCCCHHHHHCCCCEEEEEECCCCCCCEEECCCCEECCCCCCCEEEEEHHHCCHHHHHH
QIFSQILGVRIAELGYAGLKDKNALTTQFISLPKKYAPLLEKNTSNFQEKNLKILSLNYH
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCCHHHCCEEEEEEEEC
HNKIKLGHLKGNRFFMRFKKMTPLNAQKTEQVLEQIARFGMPNYFGPQRFGKFNDNHQEG
CCEEEEEEECCCEEEEEEHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCHHHH
LKILQNQTKFAHQKLNAFLISSYQSYLFNALLSKRLEISKIISAFSVKENLEFFKQKNLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
INPNTLKALKNQAHPFKILEGDVMRHYPYGKFFDALELEKESERFLKKEAAPTGLLDGKK
CCHHHHHHHHCCCCCEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
ALYAKNLSLEIEKEFQHNLLSSHAKTLGSRRFFWVFAENVTSQYVKEKAQFELGFYLPKG
HHHCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCEEEEEEECCC
SYASALLKEIKHEKGENNDEF
HHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure
MNLNFMPLLHAYNHASIDFHFNSSARDFCVHEVPLYEFSNTGEHAVIQVRKSGLSTLEML
CCCCCCHHHHHCCCCEEEEEECCCCCCCEEECCCCEECCCCCCCEEEEEHHHCCHHHHHH
QIFSQILGVRIAELGYAGLKDKNALTTQFISLPKKYAPLLEKNTSNFQEKNLKILSLNYH
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCHHHHHHHHCCCCCHHHCCEEEEEEEEC
HNKIKLGHLKGNRFFMRFKKMTPLNAQKTEQVLEQIARFGMPNYFGPQRFGKFNDNHQEG
CCEEEEEEECCCEEEEEEHHCCCCCHHHHHHHHHHHHHCCCCCCCCCHHCCCCCCCHHHH
LKILQNQTKFAHQKLNAFLISSYQSYLFNALLSKRLEISKIISAFSVKENLEFFKQKNLN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCC
INPNTLKALKNQAHPFKILEGDVMRHYPYGKFFDALELEKESERFLKKEAAPTGLLDGKK
CCHHHHHHHHCCCCCEEEECCHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCH
ALYAKNLSLEIEKEFQHNLLSSHAKTLGSRRFFWVFAENVTSQYVKEKAQFELGFYLPKG
HHHCCCCCEEEHHHHHHHHHHHHHHHHCCCEEEEEEHHHHHHHHHHHHHCEEEEEEECCC
SYASALLKEIKHEKGENNDEF
HHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA