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The map label for this gene is taqIM [H]

Identifier: 188527735

GI number: 188527735

Start: 943588

End: 944736

Strand: Direct

Name: taqIM [H]

Synonym: HPSH_04790

Alternate gene names: 188527735

Gene position: 943588-944736 (Clockwise)

Preceding gene: 188527734

Following gene: 188527736

Centisome position: 58.66

GC content: 36.21

Gene sequence:

>1149_bases
TTGTATCAATTAGATATTAAAACTTTAGGGCAGGTTTTCACCCCTAAAAATATAGTGGATTTCATGCTCACTCTCAAACA
CAATCATGGGAGTGTTTTAGAACCAAGCGCGGGCGATGGGAGTTTTTTAAAGCGCTTAAAAAAGGCTGTAGGGATTGAAA
TCGATCCTAAAATTTGCCCTAAAAATGCCCTTTGCATGGACTTTTTTGACTACCCTTTAGAAAATCAATTTGACACCATT
ATTGGCAACCCGCCCTATGTCAAACACAAGGATATTGCACCAAGCACTAAAGAAAAACTCAATTACAGCCTTTTTGATGA
AAGGAGTAATCTATACTTGTTTTTCATAGAAAAAGCGATCAAGCATTTAAAACCTAAAGGCGAATTGATTTTCATTACCC
CAAGGGATTTTTTAAAATCCACTTCCAGCGTGAAATTAAACGAATTGATTTACAAAGAAGGCACGATAACGCATTTTTTT
GAATTGGGCGATCAAAAGGTTTTCCCAAACGCCATGCCTAATTGCGTGATTTTTCGTTTTTGTAAGGGCAATTTCAGTAG
AATCACCAACGATTGTTTGCAATTCGTGTGCAAAAAAGGTATTTTATATTTTCTCAACCAATCTTACACGCAAAAATTAA
GCGAGGTTTTTAAGGTTAAAGTGGGGGCAGTGAGCGGGTGCGATAAGATTTTTAAAAATGAAAAATACGGGAATTTAGAA
TTTGTCACCTCAATCACGAAAAGAACCAATGTTTTAGAAAAAATGGTTTTTGTCAATAAGCCTAATGATTATTTACTCCA
GCATAAAGATAGCTTGATGCAAAGAAAGATCAAAAAATTCAATGAAGCCAACTGGTTTGAATGGGGTAGAATGCATCACA
TATCCCCTAAAAAACGCATTTATGTCAACACTAAAACGCGCCAAAAAAACCCCTTTTTTATCCACCAATGCCCGAATTAT
GACGGCTCTATTTTAGCGCTATTCCCTTATAACCAAAACCTGGATTTACAAAACCTCTGCGATAAACTCAACGCTATCAA
CTGGCAAGAATTAGGCTTTGTGTGCGATGGGCGTTTTTTGTTTTCGCAACGCTCTTTAGAAAACGCTCTTTTGCCTAAAG
ATTTTTTAAATGAGGATAAAACTTGTTAG

Upstream 100 bases:

>100_bases
TTTGATGGGAGTAAAAACTTCATCTTAAGCGCTTTAGCCAAAAGTGTTAAGTTACGAGCTAATATTTATTTAGCATTCAA
AGAATTTTTTGGAGAGTTTT

Downstream 100 bases:

>100_bases
AAACTTTGCAATTAAACCCTGAGCAATTAAAAGCGGCCAAGGCTTTGAAAGGGTATAATTTAGTGATTGCGAGCGCTGGC
ACAGGAAAGACTTCTACGAT

Product: type II DNA modification enzyme (methyltransferase)

Products: NA

Alternate protein names: M.TaqI; Adenine-specific methyltransferase TaqI [H]

Number of amino acids: Translated: 382; Mature: 382

Protein sequence:

>382_residues
MYQLDIKTLGQVFTPKNIVDFMLTLKHNHGSVLEPSAGDGSFLKRLKKAVGIEIDPKICPKNALCMDFFDYPLENQFDTI
IGNPPYVKHKDIAPSTKEKLNYSLFDERSNLYLFFIEKAIKHLKPKGELIFITPRDFLKSTSSVKLNELIYKEGTITHFF
ELGDQKVFPNAMPNCVIFRFCKGNFSRITNDCLQFVCKKGILYFLNQSYTQKLSEVFKVKVGAVSGCDKIFKNEKYGNLE
FVTSITKRTNVLEKMVFVNKPNDYLLQHKDSLMQRKIKKFNEANWFEWGRMHHISPKKRIYVNTKTRQKNPFFIHQCPNY
DGSILALFPYNQNLDLQNLCDKLNAINWQELGFVCDGRFLFSQRSLENALLPKDFLNEDKTC

Sequences:

>Translated_382_residues
MYQLDIKTLGQVFTPKNIVDFMLTLKHNHGSVLEPSAGDGSFLKRLKKAVGIEIDPKICPKNALCMDFFDYPLENQFDTI
IGNPPYVKHKDIAPSTKEKLNYSLFDERSNLYLFFIEKAIKHLKPKGELIFITPRDFLKSTSSVKLNELIYKEGTITHFF
ELGDQKVFPNAMPNCVIFRFCKGNFSRITNDCLQFVCKKGILYFLNQSYTQKLSEVFKVKVGAVSGCDKIFKNEKYGNLE
FVTSITKRTNVLEKMVFVNKPNDYLLQHKDSLMQRKIKKFNEANWFEWGRMHHISPKKRIYVNTKTRQKNPFFIHQCPNY
DGSILALFPYNQNLDLQNLCDKLNAINWQELGFVCDGRFLFSQRSLENALLPKDFLNEDKTC
>Mature_382_residues
MYQLDIKTLGQVFTPKNIVDFMLTLKHNHGSVLEPSAGDGSFLKRLKKAVGIEIDPKICPKNALCMDFFDYPLENQFDTI
IGNPPYVKHKDIAPSTKEKLNYSLFDERSNLYLFFIEKAIKHLKPKGELIFITPRDFLKSTSSVKLNELIYKEGTITHFF
ELGDQKVFPNAMPNCVIFRFCKGNFSRITNDCLQFVCKKGILYFLNQSYTQKLSEVFKVKVGAVSGCDKIFKNEKYGNLE
FVTSITKRTNVLEKMVFVNKPNDYLLQHKDSLMQRKIKKFNEANWFEWGRMHHISPKKRIYVNTKTRQKNPFFIHQCPNY
DGSILALFPYNQNLDLQNLCDKLNAINWQELGFVCDGRFLFSQRSLENALLPKDFLNEDKTC

Specific function: This methylase recognizes the double-stranded sequence TCGA, causes specific methylation on A-4 on both strands and protects the DNA from cleavage by the TaqI endonuclease [H]

COG id: COG0827

COG function: function code L; Adenine-specific DNA methylase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the N(4)/N(6)-methyltransferase family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR002052
- InterPro:   IPR002296
- InterPro:   IPR021188
- InterPro:   IPR023135
- InterPro:   IPR011639 [H]

Pfam domain/function: PF07669 Eco57I [H]

EC number: =2.1.1.72 [H]

Molecular weight: Translated: 44465; Mature: 44465

Theoretical pI: Translated: 9.45; Mature: 9.45

Prosite motif: PS00092 N6_MTASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.9 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
2.9 %Cys     (Mature Protein)
1.8 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MYQLDIKTLGQVFTPKNIVDFMLTLKHNHGSVLEPSAGDGSFLKRLKKAVGIEIDPKICP
CEEECHHHHHHHCCCHHHHHHHHEEECCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCC
KNALCMDFFDYPLENQFDTIIGNPPYVKHKDIAPSTKEKLNYSLFDERSNLYLFFIEKAI
CCCEEHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCHHHHCCHHEECCCCCEEEEEHHHHH
KHLKPKGELIFITPRDFLKSTSSVKLNELIYKEGTITHFFELGDQKVFPNAMPNCVIFRF
HHCCCCCCEEEECCHHHHCCCCCCCHHHHHHCCCCEEEHHHCCCCCCCCCCCCCEEEEEE
CKGNFSRITNDCLQFVCKKGILYFLNQSYTQKLSEVFKVKVGAVSGCDKIFKNEKYGNLE
CCCCHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHH
FVTSITKRTNVLEKMVFVNKPNDYLLQHKDSLMQRKIKKFNEANWFEWGRMHHISPKKRI
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCCEECCCCCCEE
YVNTKTRQKNPFFIHQCPNYDGSILALFPYNQNLDLQNLCDKLNAINWQELGFVCDGRFL
EEECCCCCCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHCCCCHHHCCEEECCCCE
FSQRSLENALLPKDFLNEDKTC
EHHHHHHHHCCCHHHCCCCCCC
>Mature Secondary Structure
MYQLDIKTLGQVFTPKNIVDFMLTLKHNHGSVLEPSAGDGSFLKRLKKAVGIEIDPKICP
CEEECHHHHHHHCCCHHHHHHHHEEECCCCCEECCCCCCHHHHHHHHHHHCCCCCCCCCC
KNALCMDFFDYPLENQFDTIIGNPPYVKHKDIAPSTKEKLNYSLFDERSNLYLFFIEKAI
CCCEEHHHHCCCCCCCCCHHCCCCCCCCCCCCCCCHHHHCCHHEECCCCCEEEEEHHHHH
KHLKPKGELIFITPRDFLKSTSSVKLNELIYKEGTITHFFELGDQKVFPNAMPNCVIFRF
HHCCCCCCEEEECCHHHHCCCCCCCHHHHHHCCCCEEEHHHCCCCCCCCCCCCCEEEEEE
CKGNFSRITNDCLQFVCKKGILYFLNQSYTQKLSEVFKVKVGAVSGCDKIFKNEKYGNLE
CCCCHHHHHHHHHHHHHHCCHHHHHCHHHHHHHHHHHHHHHCCCHHHHHHHCCCCCCCHH
FVTSITKRTNVLEKMVFVNKPNDYLLQHKDSLMQRKIKKFNEANWFEWGRMHHISPKKRI
HHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHCCCCCHHCCCEECCCCCCEE
YVNTKTRQKNPFFIHQCPNYDGSILALFPYNQNLDLQNLCDKLNAINWQELGFVCDGRFL
EEECCCCCCCCEEEEECCCCCCCEEEEECCCCCCCHHHHHHHHCCCCHHHCCEEECCCCE
FSQRSLENALLPKDFLNEDKTC
EHHHHHHHHCCCHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2827113; 1551602; 7971991 [H]