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The map label for this gene is folD [H]

Identifier: 188527576

GI number: 188527576

Start: 780368

End: 781240

Strand: Direct

Name: folD [H]

Synonym: HPSH_03990

Alternate gene names: 188527576

Gene position: 780368-781240 (Clockwise)

Preceding gene: 188527575

Following gene: 188527577

Centisome position: 48.51

GC content: 42.15

Gene sequence:

>873_bases
ATGCCAAATAGAGGCGTTGTTTTATTAGACGGGCAAGCGTTAGCTTATGATATAGAAAAAGATTTGAAAAATAAAATCCA
AATAATAACCGCGCAAACGCATAAACGCCCCAAACTAGCCGTGATTTTAGTGGGGAAAGATCCCGCTAGTATCACTTATG
TCAATATGAAGATCAAAGCATGCGAAAGGGTGGGCATGGATTTTGACTTAAAAACCCTCCAAGAAAATATTACTGAAACT
GAATTACTGTCCTTGATTAAAGATTACAATACCGATCAAAGCATTTCAGGCGTTTTAGTCCAGCTCCCCCTACCCAGACA
CATTGATACTAAAATGGTTTTAGAAGCCATTGACCCCAGTAAAGATGTGGATGGTTTCCACCCCCTTAATATCGGCAAGC
TCTGCACTCAAAAAGAATCGTTCCTGCCAGCTACCCCTATAGGTGTGATGCGTCTTTTAGAGCATTATCATATTGAAATC
AAGGGCAAGGATGTGGCGATTATTGGGGCGAGCAATATCATTGGAAAACCCTTAAGCATGCTCATGTTAAACGCTGGGGC
TAGCGTGAGCGTGTGCCATATTTTGACTAAAGACATTAGCTTTTACACCCAAAACGCTGATATTGTCTGCGTGGGCGTGG
GTAAGCCTGATTTGATTAAAGCGAGCATGTTAAAAAAAGGGGCTGTAGTGGTGGATATTGGGATCAATCATTTGAATGAT
GGGCGTATCGTGGGCGATGTGGATTTTAACAACGCGCAAAAAGTTGCCGGTTTTATCACGCCTGTGCCTAAAGGCGTGGG
GCCTATGACGATCGTTTCGCTTTTAGAAAACACTCTCATCGCTTTTGAAAAACAACAAAGGAAGGGATTTTAA

Upstream 100 bases:

>100_bases
CATGGGGCTAATCTTAAAGATTGACAAACTGAGTTTTTATCCATTAAACGCATGATTTTTGCTACAATAATAGGATTTTA
ATGGATAAAGGACAATAAGC

Downstream 100 bases:

>100_bases
ATGAAATTTTTACGCTCTGTTTATGCGTTTTGCTCCAGTTGGGTAGGGACGATCATTATTGTGCTGTTGGTTATCTTTTT
TGTCGCACAAGCCTTTATCA

Product: bifunctional 5,10-methylene-tetrahydrofolate dehydrogenase/ 5,10-methylene-tetrahydrofolate cyclohydrolase

Products: NA

Alternate protein names: Methylenetetrahydrofolate dehydrogenase; Methenyltetrahydrofolate cyclohydrolase [H]

Number of amino acids: Translated: 290; Mature: 289

Protein sequence:

>290_residues
MPNRGVVLLDGQALAYDIEKDLKNKIQIITAQTHKRPKLAVILVGKDPASITYVNMKIKACERVGMDFDLKTLQENITET
ELLSLIKDYNTDQSISGVLVQLPLPRHIDTKMVLEAIDPSKDVDGFHPLNIGKLCTQKESFLPATPIGVMRLLEHYHIEI
KGKDVAIIGASNIIGKPLSMLMLNAGASVSVCHILTKDISFYTQNADIVCVGVGKPDLIKASMLKKGAVVVDIGINHLND
GRIVGDVDFNNAQKVAGFITPVPKGVGPMTIVSLLENTLIAFEKQQRKGF

Sequences:

>Translated_290_residues
MPNRGVVLLDGQALAYDIEKDLKNKIQIITAQTHKRPKLAVILVGKDPASITYVNMKIKACERVGMDFDLKTLQENITET
ELLSLIKDYNTDQSISGVLVQLPLPRHIDTKMVLEAIDPSKDVDGFHPLNIGKLCTQKESFLPATPIGVMRLLEHYHIEI
KGKDVAIIGASNIIGKPLSMLMLNAGASVSVCHILTKDISFYTQNADIVCVGVGKPDLIKASMLKKGAVVVDIGINHLND
GRIVGDVDFNNAQKVAGFITPVPKGVGPMTIVSLLENTLIAFEKQQRKGF
>Mature_289_residues
PNRGVVLLDGQALAYDIEKDLKNKIQIITAQTHKRPKLAVILVGKDPASITYVNMKIKACERVGMDFDLKTLQENITETE
LLSLIKDYNTDQSISGVLVQLPLPRHIDTKMVLEAIDPSKDVDGFHPLNIGKLCTQKESFLPATPIGVMRLLEHYHIEIK
GKDVAIIGASNIIGKPLSMLMLNAGASVSVCHILTKDISFYTQNADIVCVGVGKPDLIKASMLKKGAVVVDIGINHLNDG
RIVGDVDFNNAQKVAGFITPVPKGVGPMTIVSLLENTLIAFEKQQRKGF

Specific function: Catalyzes the oxidation of 5,10- methylenetetrahydrofolate to 5,10-methenyltetrahydrofolate and then the hydrolysis of 5,10-methenyltetrahydrofolate to 10- formyltetrahydrofolate [H]

COG id: COG0190

COG function: function code H; 5,10-methylene-tetrahydrofolate dehydrogenase/Methenyl tetrahydrofolate cyclohydrolase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tetrahydrofolate dehydrogenase/cyclohydrolase family [H]

Homologues:

Organism=Homo sapiens, GI222418558, Length=293, Percent_Identity=41.296928327645, Blast_Score=234, Evalue=1e-61,
Organism=Homo sapiens, GI94721354, Length=296, Percent_Identity=42.2297297297297, Blast_Score=226, Evalue=2e-59,
Organism=Homo sapiens, GI222136639, Length=283, Percent_Identity=37.4558303886926, Blast_Score=193, Evalue=2e-49,
Organism=Homo sapiens, GI36796743, Length=206, Percent_Identity=26.6990291262136, Blast_Score=74, Evalue=2e-13,
Organism=Escherichia coli, GI1786741, Length=274, Percent_Identity=48.9051094890511, Blast_Score=273, Evalue=6e-75,
Organism=Caenorhabditis elegans, GI17568735, Length=260, Percent_Identity=38.4615384615385, Blast_Score=173, Evalue=9e-44,
Organism=Saccharomyces cerevisiae, GI6319558, Length=287, Percent_Identity=43.5540069686411, Blast_Score=242, Evalue=6e-65,
Organism=Saccharomyces cerevisiae, GI6321643, Length=298, Percent_Identity=39.5973154362416, Blast_Score=213, Evalue=2e-56,
Organism=Saccharomyces cerevisiae, GI6322933, Length=220, Percent_Identity=25.9090909090909, Blast_Score=71, Evalue=2e-13,
Organism=Drosophila melanogaster, GI17136816, Length=293, Percent_Identity=42.6621160409556, Blast_Score=246, Evalue=1e-65,
Organism=Drosophila melanogaster, GI17136818, Length=293, Percent_Identity=42.6621160409556, Blast_Score=246, Evalue=1e-65,
Organism=Drosophila melanogaster, GI24645718, Length=285, Percent_Identity=40, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI17137370, Length=285, Percent_Identity=40, Blast_Score=208, Evalue=3e-54,
Organism=Drosophila melanogaster, GI62472483, Length=285, Percent_Identity=40, Blast_Score=208, Evalue=4e-54,
Organism=Drosophila melanogaster, GI45551871, Length=285, Percent_Identity=40, Blast_Score=208, Evalue=4e-54,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016040
- InterPro:   IPR000672
- InterPro:   IPR020630
- InterPro:   IPR020867
- InterPro:   IPR020631 [H]

Pfam domain/function: PF00763 THF_DHG_CYH; PF02882 THF_DHG_CYH_C [H]

EC number: =1.5.1.5; =3.5.4.9 [H]

Molecular weight: Translated: 31630; Mature: 31498

Theoretical pI: Translated: 8.32; Mature: 8.32

Prosite motif: PS00766 THF_DHG_CYH_1 ; PS00767 THF_DHG_CYH_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.4 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
4.5 %Cys+Met (Translated Protein)
1.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
4.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPNRGVVLLDGQALAYDIEKDLKNKIQIITAQTHKRPKLAVILVGKDPASITYVNMKIKA
CCCCCEEEECCCEEEEHHHHHHCCEEEEEEECCCCCCCEEEEEECCCCCEEEEEEEEEEE
CERVGMDFDLKTLQENITETELLSLIKDYNTDQSISGVLVQLPLPRHIDTKMVLEAIDPS
HHHCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCC
KDVDGFHPLNIGKLCTQKESFLPATPIGVMRLLEHYHIEIKGKDVAIIGASNIIGKPLSM
CCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHEEEEECCCEEEEEECCCCCCCCHHH
LMLNAGASVSVCHILTKDISFYTQNADIVCVGVGKPDLIKASMLKKGAVVVDIGINHLND
HEECCCCCEEEEHHHHHHHHEEECCCCEEEEECCCCCHHHHHHHHCCCEEEEECCCCCCC
GRIVGDVDFNNAQKVAGFITPVPKGVGPMTIVSLLENTLIAFEKQQRKGF
CEEEEEECCCCHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
PNRGVVLLDGQALAYDIEKDLKNKIQIITAQTHKRPKLAVILVGKDPASITYVNMKIKA
CCCCEEEECCCEEEEHHHHHHCCEEEEEEECCCCCCCEEEEEECCCCCEEEEEEEEEEE
CERVGMDFDLKTLQENITETELLSLIKDYNTDQSISGVLVQLPLPRHIDTKMVLEAIDPS
HHHCCCCCCHHHHHHCCCHHHHHHHHHHCCCCCCCCCEEEEECCCCCCCHHHHHHHCCCC
KDVDGFHPLNIGKLCTQKESFLPATPIGVMRLLEHYHIEIKGKDVAIIGASNIIGKPLSM
CCCCCCCCCCHHHHHCCCCCCCCCCCHHHHHHHHHEEEEECCCEEEEEECCCCCCCCHHH
LMLNAGASVSVCHILTKDISFYTQNADIVCVGVGKPDLIKASMLKKGAVVVDIGINHLND
HEECCCCCEEEEHHHHHHHHEEECCCCEEEEECCCCCHHHHHHHHCCCEEEEECCCCCCC
GRIVGDVDFNNAQKVAGFITPVPKGVGPMTIVSLLENTLIAFEKQQRKGF
CEEEEEECCCCHHHHHHEECCCCCCCCHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA