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The map label for this gene is ligA

Identifier: 188527537

GI number: 188527537

Start: 730619

End: 732589

Strand: Direct

Name: ligA

Synonym: HPSH_03795

Alternate gene names: 188527537

Gene position: 730619-732589 (Clockwise)

Preceding gene: 188527529

Following gene: 188527538

Centisome position: 45.42

GC content: 39.62

Gene sequence:

>1971_bases
ATGATAAAAAGCCAAAAAGAATATTTAGAAAGAATTGAATATTTAAACACCCTATCGCACCATTATTACAACCTTGATGA
TTCAATCGTGAGCGATGCGGTCTATGACGAACTTTATCAAGAATTAAAAGCTTATGAAGGAAAAAACCCTAATAGCATTC
AAGCCAATTCCCCTACCCAAAAAGTGGGGGCTACTACCCCCAATTCGTTCAATAAAAACCCCCATTTAATGCGGATGTGG
AGCTTAGATGATGTGTTCAATCAAAGCGAATTGCAAGCGTGGTTGCAACGCATTTTAAAAGCCTATCCTAGCGCTTCGTT
CGTGTGTTCGCCCAAACTTGATGGGGTTTCGCTCAATCTTTTGTATCAACATGGCAAGCTAGTGAAGGCGACCACTAGGG
GCAACGGCTTAGAAGGGGAATTAGTGAGCGCAAACGCTAAACACATCGCTAATATCCCCCACGCTATCGCTTATAATGGA
GAAATAGAAATCAGGGGCGAAGTGATCATTTCTAAAAAGGATTTTGACGCTTTAAACAAAGAGCGCTTAAACGCTAATGA
ACCCCTATTCGCTAACCCTAGAAATGCCGCATCAGGGAGTTTGAGACAGCTTGATAGCGAAATCACTAAAAAGCGTAAAT
TGCAATTCATTCCTTGGGGCGTGGGCAAGCATTCTTTAAATTTCATAAGCTTTAAGGAGTGTTTGGATTTTATTGTTTCG
CTAGGTTTTAGCGCTATTCAATACTTAAGCCTAAACAAAAACCACCAAGAAATAGAAGAAAATTACCACACCCTGATTAA
AGAAAGGGAGGGCTTTTTCGCCCTTTTAGACGGCATGGTGATCGTTGTGAATGAATTAAATATTCAAAAGGAGCTAGGCT
ACACGCAAAAATCCCCTAAATTCGCTTGCGCTTATAAATTCCCGGCTCTAGAAAAACACACAAAAATTGTAGGAATCATT
AACCAAGTGGGGCGCAGCGGGGCGATCACACCGGTCGCTGTTTTAGAGCCTGTAGAAATTGCTGGAGCTATCATTACGAA
AGCGACCTTACACAATTATTCTGAAATTGAAAAAAAGAATATCATGCTCAATGATAGGGTTGTGGTCATTAGAAGCGGCG
ATGTGATCCCTAAAATCATCAAACCTTTAGAATCTTATAGGGACGGCTCGCAACATAAAATTGAACGCCCCAAAGTTTGC
CCTATATGTTCGCATGAGCTTTTATGCGAAGAGATTTTTACTTATTGTCAAAACTTAAACTGCCCGGCAAGGTTGAAAGA
AAGCTTGATTCATTTCGCTTCTAAAGACGCTTTAAACATTCAAGGCCTAGGCGATAAAGTCATAGAGCAACTTTTTGAAG
AAAAACTCATTTTTAACGCTCTGGATTTGTATGCTTTAAAATTAGAAGATTTAATGCGGCTAGACAAATTTAAAATTAAA
AAAGCTCAAAATCTATTGGACGCTATTCAAAAAAGCAAAAACCCTCCCTTATGGCGTTTGATTAACGCTTTAGGGATTGA
GCATATCGGCAAGGGAGCGAGTAAAACGCTGGCTAGATACGGCTTAAATGTGTTAGAAAAAAGCGAAGCCGAATTTTTAG
AAATGGAAGGCTTTGGGGTGGAAATGGCGCGCTCTTTAGTCAATTTTTATGCGAGCAATCAGGAATTTATCCGATCGTTA
TTTGATTTGTTAAACCCTAAAAACAGCGATATGGCTGAAGAAAAGCAAGAAAGCTCTTCTGTTTTCAGCAATAAAACGAT
TGTTTTAACCGGCACGCTTTCTAAACCACGGCAAGAATACGCTCAAATGTTAGAAAATTTAGGGGCAAAAATTTCTTCAA
GCGTGAGCGCTAAAACCGATTTTTTAATCGTTGGAGAAAACGCCGGCTCAAAACTCGCTCTAGCAAAAAAACATGGCGTG
AGCGTTTTGAATGAAGAAGAGTTGTTAAAGCGCCTTAAAGAATTTGATTGA

Upstream 100 bases:

>100_bases
GTTTTGTCAATGCCTCTTACCACCTAATTTCCCCTAAAGCCCTATCAAAGATTAAATGTTATAATACTACCCTAATTTAG
TTTAAAAGGTTTTTACAAAC

Downstream 100 bases:

>100_bases
ATTCTAAAGAAAAATTCCAATTTTCATTCTTAATGAATTTACTCACAAGATCCTATTTTATAGTAGAATGCGAAAAATAA
AGATACTTTAAGGAGCAATA

Product: NAD-dependent DNA ligase LigA

Products: NA

Alternate protein names: Polydeoxyribonucleotide synthase [NAD+] [H]

Number of amino acids: Translated: 656; Mature: 656

Protein sequence:

>656_residues
MIKSQKEYLERIEYLNTLSHHYYNLDDSIVSDAVYDELYQELKAYEGKNPNSIQANSPTQKVGATTPNSFNKNPHLMRMW
SLDDVFNQSELQAWLQRILKAYPSASFVCSPKLDGVSLNLLYQHGKLVKATTRGNGLEGELVSANAKHIANIPHAIAYNG
EIEIRGEVIISKKDFDALNKERLNANEPLFANPRNAASGSLRQLDSEITKKRKLQFIPWGVGKHSLNFISFKECLDFIVS
LGFSAIQYLSLNKNHQEIEENYHTLIKEREGFFALLDGMVIVVNELNIQKELGYTQKSPKFACAYKFPALEKHTKIVGII
NQVGRSGAITPVAVLEPVEIAGAIITKATLHNYSEIEKKNIMLNDRVVVIRSGDVIPKIIKPLESYRDGSQHKIERPKVC
PICSHELLCEEIFTYCQNLNCPARLKESLIHFASKDALNIQGLGDKVIEQLFEEKLIFNALDLYALKLEDLMRLDKFKIK
KAQNLLDAIQKSKNPPLWRLINALGIEHIGKGASKTLARYGLNVLEKSEAEFLEMEGFGVEMARSLVNFYASNQEFIRSL
FDLLNPKNSDMAEEKQESSSVFSNKTIVLTGTLSKPRQEYAQMLENLGAKISSSVSAKTDFLIVGENAGSKLALAKKHGV
SVLNEEELLKRLKEFD

Sequences:

>Translated_656_residues
MIKSQKEYLERIEYLNTLSHHYYNLDDSIVSDAVYDELYQELKAYEGKNPNSIQANSPTQKVGATTPNSFNKNPHLMRMW
SLDDVFNQSELQAWLQRILKAYPSASFVCSPKLDGVSLNLLYQHGKLVKATTRGNGLEGELVSANAKHIANIPHAIAYNG
EIEIRGEVIISKKDFDALNKERLNANEPLFANPRNAASGSLRQLDSEITKKRKLQFIPWGVGKHSLNFISFKECLDFIVS
LGFSAIQYLSLNKNHQEIEENYHTLIKEREGFFALLDGMVIVVNELNIQKELGYTQKSPKFACAYKFPALEKHTKIVGII
NQVGRSGAITPVAVLEPVEIAGAIITKATLHNYSEIEKKNIMLNDRVVVIRSGDVIPKIIKPLESYRDGSQHKIERPKVC
PICSHELLCEEIFTYCQNLNCPARLKESLIHFASKDALNIQGLGDKVIEQLFEEKLIFNALDLYALKLEDLMRLDKFKIK
KAQNLLDAIQKSKNPPLWRLINALGIEHIGKGASKTLARYGLNVLEKSEAEFLEMEGFGVEMARSLVNFYASNQEFIRSL
FDLLNPKNSDMAEEKQESSSVFSNKTIVLTGTLSKPRQEYAQMLENLGAKISSSVSAKTDFLIVGENAGSKLALAKKHGV
SVLNEEELLKRLKEFD
>Mature_656_residues
MIKSQKEYLERIEYLNTLSHHYYNLDDSIVSDAVYDELYQELKAYEGKNPNSIQANSPTQKVGATTPNSFNKNPHLMRMW
SLDDVFNQSELQAWLQRILKAYPSASFVCSPKLDGVSLNLLYQHGKLVKATTRGNGLEGELVSANAKHIANIPHAIAYNG
EIEIRGEVIISKKDFDALNKERLNANEPLFANPRNAASGSLRQLDSEITKKRKLQFIPWGVGKHSLNFISFKECLDFIVS
LGFSAIQYLSLNKNHQEIEENYHTLIKEREGFFALLDGMVIVVNELNIQKELGYTQKSPKFACAYKFPALEKHTKIVGII
NQVGRSGAITPVAVLEPVEIAGAIITKATLHNYSEIEKKNIMLNDRVVVIRSGDVIPKIIKPLESYRDGSQHKIERPKVC
PICSHELLCEEIFTYCQNLNCPARLKESLIHFASKDALNIQGLGDKVIEQLFEEKLIFNALDLYALKLEDLMRLDKFKIK
KAQNLLDAIQKSKNPPLWRLINALGIEHIGKGASKTLARYGLNVLEKSEAEFLEMEGFGVEMARSLVNFYASNQEFIRSL
FDLLNPKNSDMAEEKQESSSVFSNKTIVLTGTLSKPRQEYAQMLENLGAKISSSVSAKTDFLIVGENAGSKLALAKKHGV
SVLNEEELLKRLKEFD

Specific function: DNA ligase that catalyzes the formation of phosphodiester linkages between 5'-phosphoryl and 3'-hydroxyl groups in double-stranded DNA using NAD as a coenzyme and as the energy source for the reaction. It is essential for DNA replication and repair of dam

COG id: COG0272

COG function: function code L; NAD-dependent DNA ligase (contains BRCT domain type II)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 BRCT domain [H]

Homologues:

Organism=Escherichia coli, GI1788750, Length=668, Percent_Identity=37.874251497006, Blast_Score=432, Evalue=1e-122,
Organism=Escherichia coli, GI87082305, Length=555, Percent_Identity=21.0810810810811, Blast_Score=100, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001357
- InterPro:   IPR018239
- InterPro:   IPR004150
- InterPro:   IPR001679
- InterPro:   IPR013839
- InterPro:   IPR013840
- InterPro:   IPR003583
- InterPro:   IPR012340
- InterPro:   IPR016027
- InterPro:   IPR010994 [H]

Pfam domain/function: PF00533 BRCT; PF01653 DNA_ligase_aden; PF03120 DNA_ligase_OB [H]

EC number: =6.5.1.2 [H]

Molecular weight: Translated: 73868; Mature: 73868

Theoretical pI: Translated: 7.83; Mature: 7.83

Prosite motif: PS50172 BRCT ; PS01055 DNA_LIGASE_N1 ; PS01056 DNA_LIGASE_N2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKSQKEYLERIEYLNTLSHHYYNLDDSIVSDAVYDELYQELKAYEGKNPNSIQANSPTQ
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
KVGATTPNSFNKNPHLMRMWSLDDVFNQSELQAWLQRILKAYPSASFVCSPKLDGVSLNL
HCCCCCCCCCCCCCCEEEEECHHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEE
LYQHGKLVKATTRGNGLEGELVSANAKHIANIPHAIAYNGEIEIRGEVIISKKDFDALNK
EEECCCEEEEECCCCCCCCCEECCCCHHHHCCCCEEEECCEEEEEEEEEEECCCHHHHHH
ERLNANEPLFANPRNAASGSLRQLDSEITKKRKLQFIPWGVGKHSLNFISFKECLDFIVS
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHHHH
LGFSAIQYLSLNKNHQEIEENYHTLIKEREGFFALLDGMVIVVNELNIQKELGYTQKSPK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHCCEEEEEECCCHHHHCCCCCCCCC
FACAYKFPALEKHTKIVGIINQVGRSGAITPVAVLEPVEIAGAIITKATLHNYSEIEKKN
EEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHC
IMLNDRVVVIRSGDVIPKIIKPLESYRDGSQHKIERPKVCPICSHELLCEEIFTYCQNLN
EEEECEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
CPARLKESLIHFASKDALNIQGLGDKVIEQLFEEKLIFNALDLYALKLEDLMRLDKFKIK
CHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAQNLLDAIQKSKNPPLWRLINALGIEHIGKGASKTLARYGLNVLEKSEAEFLEMEGFGV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHCCHHHHHHCCCCH
EMARSLVNFYASNQEFIRSLFDLLNPKNSDMAEEKQESSSVFSNKTIVLTGTLSKPRQEY
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHH
AQMLENLGAKISSSVSAKTDFLIVGENAGSKLALAKKHGVSVLNEEELLKRLKEFD
HHHHHHHCCHHHCCCCCCCCEEEEECCCCCEEEEEHHCCCCCCCHHHHHHHHHCCC
>Mature Secondary Structure
MIKSQKEYLERIEYLNTLSHHYYNLDDSIVSDAVYDELYQELKAYEGKNPNSIQANSPTQ
CCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHH
KVGATTPNSFNKNPHLMRMWSLDDVFNQSELQAWLQRILKAYPSASFVCSPKLDGVSLNL
HCCCCCCCCCCCCCCEEEEECHHHHCCHHHHHHHHHHHHHHCCCCCEEECCCCCCEEEEE
LYQHGKLVKATTRGNGLEGELVSANAKHIANIPHAIAYNGEIEIRGEVIISKKDFDALNK
EEECCCEEEEECCCCCCCCCEECCCCHHHHCCCCEEEECCEEEEEEEEEEECCCHHHHHH
ERLNANEPLFANPRNAASGSLRQLDSEITKKRKLQFIPWGVGKHSLNFISFKECLDFIVS
HHCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCEEEECCCCCCCCCHHHHHHHHHHHHHH
LGFSAIQYLSLNKNHQEIEENYHTLIKEREGFFALLDGMVIVVNELNIQKELGYTQKSPK
HHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHCCEEEEEECCCHHHHCCCCCCCCC
FACAYKFPALEKHTKIVGIINQVGRSGAITPVAVLEPVEIAGAIITKATLHNYSEIEKKN
EEEEECCCCHHHHHHHHHHHHHHCCCCCCCHHHHHCHHHHHHHHHHHHHHHHHHHHHHHC
IMLNDRVVVIRSGDVIPKIIKPLESYRDGSQHKIERPKVCPICSHELLCEEIFTYCQNLN
EEEECEEEEEECCCCHHHHHHHHHHHCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHCCC
CPARLKESLIHFASKDALNIQGLGDKVIEQLFEEKLIFNALDLYALKLEDLMRLDKFKIK
CHHHHHHHHHHHHCCCCCEECCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
KAQNLLDAIQKSKNPPLWRLINALGIEHIGKGASKTLARYGLNVLEKSEAEFLEMEGFGV
HHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHCHHHHHHCCHHHHHHCCCCH
EMARSLVNFYASNQEFIRSLFDLLNPKNSDMAEEKQESSSVFSNKTIVLTGTLSKPRQEY
HHHHHHHHHHCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHH
AQMLENLGAKISSSVSAKTDFLIVGENAGSKLALAKKHGVSVLNEEELLKRLKEFD
HHHHHHHCCHHHCCCCCCCCEEEEECCCCCEEEEEHHCCCCCCCHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA