Click here to switch to the map view.

The map label for this gene is dus [H]

Identifier: 188527429

GI number: 188527429

Start: 610915

End: 611901

Strand: Direct

Name: dus [H]

Synonym: HPSH_03225

Alternate gene names: 188527429

Gene position: 610915-611901 (Clockwise)

Preceding gene: 188527428

Following gene: 188527430

Centisome position: 37.98

GC content: 40.73

Gene sequence:

>987_bases
ATGGACTTTAAGAATAAAAAATGGCTTTTTCTAGCCCCTTTGGCAGGCTATACGGATTTGCCTTTCAGGAGTGTGGTGAA
AAAATTTGGCGTGGATGTTACCACAAGCGAAATGGTGAGCTCGCATTCGTTAGTGTATGCGTTTGATAAAACTTCTAAAA
TGTTAGAAAAATCCCATTTAGAAGATCATTTCATGGCGCAAATTTCAGGCTCTAAAGAGGGCGTAGTCAAAGAAGCGGTG
GAGAAAATCAACGCTTTAGAGCATGTGAGCGGGATTGATTTTAATTGCGGTTGCCCTGCCCCTAAAGTGGCTAATCATGG
TAATGGTAGCGGGCTATTGAAGGATTTAAACCACTTAGTGAAGCTTTTAAAAATCATCAGAGAAAACACCAATAAAAAAA
TCACAAGCGTGAAAGTGCGTTTAGGCTTTGATCAAAAAATCCCTAAAGAAATCGCTCATGCCTTAAATGATGCACCGGTG
GATTATGTGGTGGTGCATGGGAGGACACGAAGCGACAAATACCAAAAAGACAAAATAGATTATAAAAGCATCGCTTTAAT
GAAAGGGATTTTAAAAAAGCCGGTGATAGCCAATGGCGAAATTGACAGCGTGAAAAAAGCCTTTGAAGTCTTGCAAATCA
CGCAAGCTGAAGGGCTAATGATAGGGCGAGCAGCCTTAAGAGCCCCATGGATATTTTGGCAAATCAGAAACAACACCACC
GAATTGCCCGCAGTCGTGAAAAAAGACCTGGTTTTAGAACATTTTGATAAAATGGTGGAGTTTTATGGGGATAGGGGGGT
GATCATGTTTAGGAAAAATTTGCACGCTTACGCTAAGGGCGAAATGCAAGCGAGTGCGTTTCGCAACTGCGTCAATACCC
TTACAGAAATAAAGAGCATGCGAGAGAGTATAGAGGAATTTTTTAATCAAGAAATGTTGCAAAGTGAAGTGCCATTATGG
GTAGAATTGAATCAAAAAAGCGTTTGA

Upstream 100 bases:

>100_bases
TCTATAAAAGAGCTTCATTTCAATCAAGCGATAAGTTTTTAAAGCGCTTTTTAACCTTGATTGTGTCAAAATACAGCAAA
AAACTTTGATATGAGAATGA

Downstream 100 bases:

>100_bases
AAGCGCTTGTTTTTTTAGCCAGTTTGGGGGTTTTGTGGGGAAATGCCGCTGAAAAAACGCCTTTTTTTAAAACGAAAAAC
CACATTTATTTGGGTTTTAG

Product: transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MDFKNKKWLFLAPLAGYTDLPFRSVVKKFGVDVTTSEMVSSHSLVYAFDKTSKMLEKSHLEDHFMAQISGSKEGVVKEAV
EKINALEHVSGIDFNCGCPAPKVANHGNGSGLLKDLNHLVKLLKIIRENTNKKITSVKVRLGFDQKIPKEIAHALNDAPV
DYVVVHGRTRSDKYQKDKIDYKSIALMKGILKKPVIANGEIDSVKKAFEVLQITQAEGLMIGRAALRAPWIFWQIRNNTT
ELPAVVKKDLVLEHFDKMVEFYGDRGVIMFRKNLHAYAKGEMQASAFRNCVNTLTEIKSMRESIEEFFNQEMLQSEVPLW
VELNQKSV

Sequences:

>Translated_328_residues
MDFKNKKWLFLAPLAGYTDLPFRSVVKKFGVDVTTSEMVSSHSLVYAFDKTSKMLEKSHLEDHFMAQISGSKEGVVKEAV
EKINALEHVSGIDFNCGCPAPKVANHGNGSGLLKDLNHLVKLLKIIRENTNKKITSVKVRLGFDQKIPKEIAHALNDAPV
DYVVVHGRTRSDKYQKDKIDYKSIALMKGILKKPVIANGEIDSVKKAFEVLQITQAEGLMIGRAALRAPWIFWQIRNNTT
ELPAVVKKDLVLEHFDKMVEFYGDRGVIMFRKNLHAYAKGEMQASAFRNCVNTLTEIKSMRESIEEFFNQEMLQSEVPLW
VELNQKSV
>Mature_328_residues
MDFKNKKWLFLAPLAGYTDLPFRSVVKKFGVDVTTSEMVSSHSLVYAFDKTSKMLEKSHLEDHFMAQISGSKEGVVKEAV
EKINALEHVSGIDFNCGCPAPKVANHGNGSGLLKDLNHLVKLLKIIRENTNKKITSVKVRLGFDQKIPKEIAHALNDAPV
DYVVVHGRTRSDKYQKDKIDYKSIALMKGILKKPVIANGEIDSVKKAFEVLQITQAEGLMIGRAALRAPWIFWQIRNNTT
ELPAVVKKDLVLEHFDKMVEFYGDRGVIMFRKNLHAYAKGEMQASAFRNCVNTLTEIKSMRESIEEFFNQEMLQSEVPLW
VELNQKSV

Specific function: Catalyzes the synthesis of dihydrouridine, a modified base found in the D-loop of most tRNAs [H]

COG id: COG0042

COG function: function code J; tRNA-dihydrouridine synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the dus family [H]

Homologues:

Organism=Homo sapiens, GI239788483, Length=246, Percent_Identity=31.7073170731707, Blast_Score=117, Evalue=2e-26,
Organism=Homo sapiens, GI239788462, Length=246, Percent_Identity=31.7073170731707, Blast_Score=115, Evalue=5e-26,
Organism=Homo sapiens, GI31742496, Length=224, Percent_Identity=27.6785714285714, Blast_Score=105, Evalue=4e-23,
Organism=Homo sapiens, GI8923374, Length=252, Percent_Identity=25.7936507936508, Blast_Score=68, Evalue=1e-11,
Organism=Escherichia coli, GI1789660, Length=317, Percent_Identity=33.1230283911672, Blast_Score=147, Evalue=7e-37,
Organism=Escherichia coli, GI1788462, Length=155, Percent_Identity=39.3548387096774, Blast_Score=107, Evalue=8e-25,
Organism=Caenorhabditis elegans, GI17543114, Length=275, Percent_Identity=26.5454545454545, Blast_Score=94, Evalue=8e-20,
Organism=Caenorhabditis elegans, GI25144369, Length=218, Percent_Identity=27.0642201834862, Blast_Score=91, Evalue=9e-19,
Organism=Caenorhabditis elegans, GI17510279, Length=246, Percent_Identity=27.6422764227642, Blast_Score=77, Evalue=1e-14,
Organism=Caenorhabditis elegans, GI17507177, Length=228, Percent_Identity=28.0701754385965, Blast_Score=74, Evalue=1e-13,
Organism=Saccharomyces cerevisiae, GI6323437, Length=230, Percent_Identity=25.6521739130435, Blast_Score=89, Evalue=1e-18,
Organism=Saccharomyces cerevisiae, GI6323433, Length=265, Percent_Identity=25.6603773584906, Blast_Score=87, Evalue=5e-18,
Organism=Drosophila melanogaster, GI24585320, Length=284, Percent_Identity=28.8732394366197, Blast_Score=120, Evalue=1e-27,
Organism=Drosophila melanogaster, GI19921524, Length=229, Percent_Identity=27.9475982532751, Blast_Score=97, Evalue=1e-20,
Organism=Drosophila melanogaster, GI24580595, Length=244, Percent_Identity=25, Blast_Score=69, Evalue=5e-12,
Organism=Drosophila melanogaster, GI19920448, Length=244, Percent_Identity=25, Blast_Score=69, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR013785
- InterPro:   IPR001269
- InterPro:   IPR018517 [H]

Pfam domain/function: PF01207 Dus [H]

EC number: NA

Molecular weight: Translated: 37127; Mature: 37127

Theoretical pI: Translated: 9.66; Mature: 9.66

Prosite motif: PS00435 PEROXIDASE_1 ; PS01136 UPF0034

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
3.4 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
3.4 %Met     (Mature Protein)
4.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDFKNKKWLFLAPLAGYTDLPFRSVVKKFGVDVTTSEMVSSHSLVYAFDKTSKMLEKSHL
CCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCEEEEECHHHHHHHHHHH
EDHFMAQISGSKEGVVKEAVEKINALEHVSGIDFNCGCPAPKVANHGNGSGLLKDLNHLV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
KLLKIIRENTNKKITSVKVRLGFDQKIPKEIAHALNDAPVDYVVVHGRTRSDKYQKDKID
HHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHCCC
YKSIALMKGILKKPVIANGEIDSVKKAFEVLQITQAEGLMIGRAALRAPWIFWQIRNNTT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEHHHHHHCCEEEEEECCCCC
ELPAVVKKDLVLEHFDKMVEFYGDRGVIMFRKNLHAYAKGEMQASAFRNCVNTLTEIKSM
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHH
RESIEEFFNQEMLQSEVPLWVELNQKSV
HHHHHHHHHHHHHHHCCCEEEEECCCCC
>Mature Secondary Structure
MDFKNKKWLFLAPLAGYTDLPFRSVVKKFGVDVTTSEMVSSHSLVYAFDKTSKMLEKSHL
CCCCCCCEEEEECCCCCCCCCHHHHHHHHCCCCCHHHHHHCCCEEEEECHHHHHHHHHHH
EDHFMAQISGSKEGVVKEAVEKINALEHVSGIDFNCGCPAPKVANHGNGSGLLKDLNHLV
HHHHHHHHCCCCCHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHH
KLLKIIRENTNKKITSVKVRLGFDQKIPKEIAHALNDAPVDYVVVHGRTRSDKYQKDKID
HHHHHHHHCCCCEEEEEEEEECCCCCHHHHHHHHHCCCCCCEEEEECCCCCCCHHHHCCC
YKSIALMKGILKKPVIANGEIDSVKKAFEVLQITQAEGLMIGRAALRAPWIFWQIRNNTT
HHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHCCEEEHHHHHHCCEEEEEECCCCC
ELPAVVKKDLVLEHFDKMVEFYGDRGVIMFRKNLHAYAKGEMQASAFRNCVNTLTEIKSM
HHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCHHHHCCCHHHHHHHHHHHHHHHHHHH
RESIEEFFNQEMLQSEVPLWVELNQKSV
HHHHHHHHHHHHHHHCCCEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]