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The map label for this gene is tilS [H]

Identifier: 188527428

GI number: 188527428

Start: 609790

End: 610821

Strand: Direct

Name: tilS [H]

Synonym: HPSH_03220

Alternate gene names: 188527428

Gene position: 609790-610821 (Clockwise)

Preceding gene: 188527427

Following gene: 188527429

Centisome position: 37.91

GC content: 36.14

Gene sequence:

>1032_bases
ATGCTTAATGCCGATTTGATAGCGCGAGATTTTAAAACTTATTTAGAGCCTTTAAGAGAGAAGAAAAATTTATTGGGTTT
TTCAGGTGGGTTGGATTCTACTTGCTTGTTTCATCTTTTAGTTGGAGAAAATATCGCTTTTGACATCGCTTTAGTGGATT
ACAACACGCAAAAACAACGCCTTAAAATCATCCAACACGCCCAAAAACTCGCCCAAACACACCATAAAAAATGCTATATC
CATTACGCCCCCAAGATTGAGTGCAATTTTGAAATGCAAGCGAGAAAGATCCGTTATGATTTTTTTGAAACTTTGATCAA
AGAGCATTCTTACAAGCATTTGATTTTAGCGCACCACTTGAATGACAGGCTGGAATGGTTTTTGATGCAATTAAGCAAAG
GAGCCGGGCTAAACACGCTTTTAAGCTTTCAAGCTTATGAAAAAAGAGAGTTTTATGCGATCGTTCGCCCCTTGCTCTAC
ACCCCTAAAGACACCCTTAAAACGCTCGCTAGAGATCGGGAATTTTTTGAAGATGATTCTAATTCTTCTTTAAAATTCAA
ACGCAATTTTTTCAGGAAAAATTACGCTAACTCCTTGATGCAACATTACTCTAAGGGCATTATCCAAAGTTTTAAGTTTT
TAGATAAAGAAAAAGAACGGCTTTACCCTTTGATGGTCGTTTCACAAATGCATGGAATCACTTTTTTTAAGTATTCGCAA
AATGCGCTTTTTATGGTGGATAAAATCTTAAAGCAAAAGGGGTATGTGTTGAGTTTTTCTCAAAAAGAAGAAATCAAACG
CAATTTTTTTAGCCTAGAAATCGCTCAAAAATTCATCATTGAAAGCGATAATGAGCATGCGTTTATCGCCCTTAAACCTC
AAAAAACTTTAAGCATGCCAAAGGATTTTAAAGACAAAGCCAGGAGGTTGAATATCCCTAAACGCTTACGGCCTGTTTTA
TACGCAGAGTTTTTAAAACAACCAACGCATGATTTTTTAACCCGCTTTAAACAGAGTTTAACGGATCTATAA

Upstream 100 bases:

>100_bases
AAGCTCAGGAATGAAATTTTAGGCATAGAAGGTATGAGCTTGTTGAAAAGCATTCTGACTCGCAAGGAAGTTTCATGAAA
GAATGCGATGAATTAAATCT

Downstream 100 bases:

>100_bases
AAGAGCTTCATTTCAATCAAGCGATAAGTTTTTAAAGCGCTTTTTAACCTTGATTGTGTCAAAATACAGCAAAAAACTTT
GATATGAGAATGAATGGACT

Product: hypothetical protein

Products: NA

Alternate protein names: tRNA(Ile)-2-lysyl-cytidine synthase; tRNA(Ile)-lysidine synthetase [H]

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MLNADLIARDFKTYLEPLREKKNLLGFSGGLDSTCLFHLLVGENIAFDIALVDYNTQKQRLKIIQHAQKLAQTHHKKCYI
HYAPKIECNFEMQARKIRYDFFETLIKEHSYKHLILAHHLNDRLEWFLMQLSKGAGLNTLLSFQAYEKREFYAIVRPLLY
TPKDTLKTLARDREFFEDDSNSSLKFKRNFFRKNYANSLMQHYSKGIIQSFKFLDKEKERLYPLMVVSQMHGITFFKYSQ
NALFMVDKILKQKGYVLSFSQKEEIKRNFFSLEIAQKFIIESDNEHAFIALKPQKTLSMPKDFKDKARRLNIPKRLRPVL
YAEFLKQPTHDFLTRFKQSLTDL

Sequences:

>Translated_343_residues
MLNADLIARDFKTYLEPLREKKNLLGFSGGLDSTCLFHLLVGENIAFDIALVDYNTQKQRLKIIQHAQKLAQTHHKKCYI
HYAPKIECNFEMQARKIRYDFFETLIKEHSYKHLILAHHLNDRLEWFLMQLSKGAGLNTLLSFQAYEKREFYAIVRPLLY
TPKDTLKTLARDREFFEDDSNSSLKFKRNFFRKNYANSLMQHYSKGIIQSFKFLDKEKERLYPLMVVSQMHGITFFKYSQ
NALFMVDKILKQKGYVLSFSQKEEIKRNFFSLEIAQKFIIESDNEHAFIALKPQKTLSMPKDFKDKARRLNIPKRLRPVL
YAEFLKQPTHDFLTRFKQSLTDL
>Mature_343_residues
MLNADLIARDFKTYLEPLREKKNLLGFSGGLDSTCLFHLLVGENIAFDIALVDYNTQKQRLKIIQHAQKLAQTHHKKCYI
HYAPKIECNFEMQARKIRYDFFETLIKEHSYKHLILAHHLNDRLEWFLMQLSKGAGLNTLLSFQAYEKREFYAIVRPLLY
TPKDTLKTLARDREFFEDDSNSSLKFKRNFFRKNYANSLMQHYSKGIIQSFKFLDKEKERLYPLMVVSQMHGITFFKYSQ
NALFMVDKILKQKGYVLSFSQKEEIKRNFFSLEIAQKFIIESDNEHAFIALKPQKTLSMPKDFKDKARRLNIPKRLRPVL
YAEFLKQPTHDFLTRFKQSLTDL

Specific function: Ligates lysine onto the cytidine present at position 34 of the AUA codon-specific tRNA(Ile) that contains the anticodon CAU, in an ATP-dependent manner. Cytidine is converted to lysidine, thus changing the amino acid specificity of the tRNA from methionin

COG id: COG0037

COG function: function code D; Predicted ATPase of the PP-loop superfamily implicated in cell cycle control

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tRNA(Ile)-lysidine synthase family [H]

Homologues:

Organism=Escherichia coli, GI1786386, Length=196, Percent_Identity=29.0816326530612, Blast_Score=75, Evalue=5e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012094
- InterPro:   IPR012795
- InterPro:   IPR014729
- InterPro:   IPR011063 [H]

Pfam domain/function: PF01171 ATP_bind_3 [H]

EC number: NA

Molecular weight: Translated: 40810; Mature: 40810

Theoretical pI: Translated: 10.16; Mature: 10.16

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLNADLIARDFKTYLEPLREKKNLLGFSGGLDSTCLFHLLVGENIAFDIALVDYNTQKQR
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCHHHH
LKIIQHAQKLAQTHHKKCYIHYAPKIECNFEMQARKIRYDFFETLIKEHSYKHLILAHHL
HHHHHHHHHHHHHHCCEEEEEECCEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
NDRLEWFLMQLSKGAGLNTLLSFQAYEKREFYAIVRPLLYTPKDTLKTLARDREFFEDDS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
NSSLKFKRNFFRKNYANSLMQHYSKGIIQSFKFLDKEKERLYPLMVVSQMHGITFFKYSQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
NALFMVDKILKQKGYVLSFSQKEEIKRNFFSLEIAQKFIIESDNEHAFIALKPQKTLSMP
CHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCCC
KDFKDKARRLNIPKRLRPVLYAEFLKQPTHDFLTRFKQSLTDL
CCHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC
>Mature Secondary Structure
MLNADLIARDFKTYLEPLREKKNLLGFSGGLDSTCLFHLLVGENIAFDIALVDYNTQKQR
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHCCCEEEEEEEEECCCHHHH
LKIIQHAQKLAQTHHKKCYIHYAPKIECNFEMQARKIRYDFFETLIKEHSYKHLILAHHL
HHHHHHHHHHHHHHCCEEEEEECCEEEECCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHH
NDRLEWFLMQLSKGAGLNTLLSFQAYEKREFYAIVRPLLYTPKDTLKTLARDREFFEDDS
HHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCC
NSSLKFKRNFFRKNYANSLMQHYSKGIIQSFKFLDKEKERLYPLMVVSQMHGITFFKYSQ
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
NALFMVDKILKQKGYVLSFSQKEEIKRNFFSLEIAQKFIIESDNEHAFIALKPQKTLSMP
CHHHHHHHHHHCCCCEEECCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCHHHHCCC
KDFKDKARRLNIPKRLRPVLYAEFLKQPTHDFLTRFKQSLTDL
CCHHHHHHHCCCHHHHHHHHHHHHHCCCHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]