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The map label for this gene is murF [H]

Identifier: 188527417

GI number: 188527417

Start: 598645

End: 600057

Strand: Reverse

Name: murF [H]

Synonym: HPSH_03140

Alternate gene names: 188527417

Gene position: 600057-598645 (Counterclockwise)

Preceding gene: 188527418

Following gene: 188527416

Centisome position: 37.3

GC content: 37.65

Gene sequence:

>1413_bases
ATGACTTTATTGCAATGGTATCATTACAGCGTGTTTAGGATCTTAACCAAGCACCACAAGATGCGTTGGCATGGGATTTA
TTTTTTATTGCCTTTGGGGGTGTTTATCCTATCGTATGCTTTCACAATGCCGTTTGTTTTTGATTTCTTTTGCGGCGTTA
TTCAAATGCCCATGCTCATTGTTTGGGCCAAACGCAACGACAAGCCTTTAGTTTTCACGCCAAGGGTGAAGCGCTTTTTT
ATCTTCTTATTATTATTTTTAATCTTGCATGAAATCTTAAATACAGAATTAGTCCCTTTGAATGGGATTTCGCTCGCGCT
AGGCTATTTGTGTTTATTCATATTCGTTTTAAGCGCTTCTTTAATCTTTGAAAAAGTCTTATCCAAGCGGTATTTGCAAA
CCGCTAAAGATAAAATCGCCTCTTTAAAAAATTTAAAAGTCATCGCCATTACAGGAAGCTTTGGGAAAACCAGCATTAAA
AAATTCTTGCTTCAAATCTTACAAACCACATTCAACACGCATGCAAGCCCCAAAAGCGTCAATACCCTTTTAGGGCTTGC
GAATGATATTAACCAGAATTTAGACGATAGGAGTGATATCTATATCGCTGAAGCCGGAGCAAGGAATAAGGGCGATATTA
AAGAAATCACCCACCTTATTGAACCGCACCTTGCCGTGGTCGCAGAAGTGGGCGAACAGCATTTAGAATATTTTAAAACT
TTAGAAAATATTTGTGAGACTAAAGCGGAATTATTGGATTCCAAACGCTTAGAAAAAGCCTTTTGTTACTCTGTAGAAAA
AATCAATCCCTATGCCCCTAAAGACAGCCCTTTAATAGACTATTCTAGCCTGGTTAAAAACATCCAATCCACTTTAAAAG
GCACTTCTTTTGAAATGCTTATAGGTAGCGTTTGGGAAAGCTTTGAAACAAAGGTTTTAGGTAAATTCAACGCTTATAAT
ATCGCTTCAGCCATTTTAATCGCTAAGCATTTAGGCTTAGAAACAGAAAGGATCAAACGGCTTGTTTTAGAACTCAACCC
CATTGCCCATCGTTTGCAACTTTTGGAAGTGAATCAAAAAATCATTATAGACGATAGCTTTAATGGGAATTTAAAGGGCA
TGTTAGAGGGCATTCGTTTGGCGAGTTTGTATGAAGGGCGTAAAGTCATTGTAACACCGGGATTAGTGGAAAGCAATACA
GAAAGTAATGAAACTTTAGCGCAAAAAATAGATGAAGTTTTTGATGTCGCTATCATCACAGGGGAGTTGAATTCCAAAAC
GATTGCTTCACAATTAAAAACCCCCCAAAAAATCTTACTCAAGGATAAAGCGCAATTGGAAAATATCTTACAAGCCACCA
CGATTCAAGGCGATTTGATTTTATTCGCCAATGACGCCCCTAATTACATTTAG

Upstream 100 bases:

>100_bases
AAAGAGATTGAAAAACTAGTGGAGAATCATCATGCAAAGTCTTAGTTGGCTGAATTTAGCGTTTCGTTGGCTCTTTATAA
CAGGGCTTGGCTATTATATA

Downstream 100 bases:

>100_bases
GAAATGAACATGCAACATTTATACGCTCCTTGGCGCGAAAGTTATTTGAAAGAGAAAGATAAGAGTTGTGTCTTTTGTGA
AATTTCTCAAAACCCTACAA

Product: UDP-MurNac-pentapeptide presynthetase (murF)

Products: NA

Alternate protein names: D-alanyl-D-alanine-adding enzyme; UDP-MurNAc-pentapeptide synthetase [H]

Number of amino acids: Translated: 470; Mature: 469

Protein sequence:

>470_residues
MTLLQWYHYSVFRILTKHHKMRWHGIYFLLPLGVFILSYAFTMPFVFDFFCGVIQMPMLIVWAKRNDKPLVFTPRVKRFF
IFLLLFLILHEILNTELVPLNGISLALGYLCLFIFVLSASLIFEKVLSKRYLQTAKDKIASLKNLKVIAITGSFGKTSIK
KFLLQILQTTFNTHASPKSVNTLLGLANDINQNLDDRSDIYIAEAGARNKGDIKEITHLIEPHLAVVAEVGEQHLEYFKT
LENICETKAELLDSKRLEKAFCYSVEKINPYAPKDSPLIDYSSLVKNIQSTLKGTSFEMLIGSVWESFETKVLGKFNAYN
IASAILIAKHLGLETERIKRLVLELNPIAHRLQLLEVNQKIIIDDSFNGNLKGMLEGIRLASLYEGRKVIVTPGLVESNT
ESNETLAQKIDEVFDVAIITGELNSKTIASQLKTPQKILLKDKAQLENILQATTIQGDLILFANDAPNYI

Sequences:

>Translated_470_residues
MTLLQWYHYSVFRILTKHHKMRWHGIYFLLPLGVFILSYAFTMPFVFDFFCGVIQMPMLIVWAKRNDKPLVFTPRVKRFF
IFLLLFLILHEILNTELVPLNGISLALGYLCLFIFVLSASLIFEKVLSKRYLQTAKDKIASLKNLKVIAITGSFGKTSIK
KFLLQILQTTFNTHASPKSVNTLLGLANDINQNLDDRSDIYIAEAGARNKGDIKEITHLIEPHLAVVAEVGEQHLEYFKT
LENICETKAELLDSKRLEKAFCYSVEKINPYAPKDSPLIDYSSLVKNIQSTLKGTSFEMLIGSVWESFETKVLGKFNAYN
IASAILIAKHLGLETERIKRLVLELNPIAHRLQLLEVNQKIIIDDSFNGNLKGMLEGIRLASLYEGRKVIVTPGLVESNT
ESNETLAQKIDEVFDVAIITGELNSKTIASQLKTPQKILLKDKAQLENILQATTIQGDLILFANDAPNYI
>Mature_469_residues
TLLQWYHYSVFRILTKHHKMRWHGIYFLLPLGVFILSYAFTMPFVFDFFCGVIQMPMLIVWAKRNDKPLVFTPRVKRFFI
FLLLFLILHEILNTELVPLNGISLALGYLCLFIFVLSASLIFEKVLSKRYLQTAKDKIASLKNLKVIAITGSFGKTSIKK
FLLQILQTTFNTHASPKSVNTLLGLANDINQNLDDRSDIYIAEAGARNKGDIKEITHLIEPHLAVVAEVGEQHLEYFKTL
ENICETKAELLDSKRLEKAFCYSVEKINPYAPKDSPLIDYSSLVKNIQSTLKGTSFEMLIGSVWESFETKVLGKFNAYNI
ASAILIAKHLGLETERIKRLVLELNPIAHRLQLLEVNQKIIIDDSFNGNLKGMLEGIRLASLYEGRKVIVTPGLVESNTE
SNETLAQKIDEVFDVAIITGELNSKTIASQLKTPQKILLKDKAQLENILQATTIQGDLILFANDAPNYI

Specific function: Involved in cell wall formation. Catalyzes the final step in the synthesis of UDP-N-acetylmuramoyl-pentapeptide, the precursor of murein [H]

COG id: COG0770

COG function: function code M; UDP-N-acetylmuramyl pentapeptide synthase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR000713
- InterPro:   IPR005863 [H]

Pfam domain/function: PF01225 Mur_ligase; PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.10 [H]

Molecular weight: Translated: 53240; Mature: 53109

Theoretical pI: Translated: 8.94; Mature: 8.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.9 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTLLQWYHYSVFRILTKHHKMRWHGIYFLLPLGVFILSYAFTMPFVFDFFCGVIQMPMLI
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VWAKRNDKPLVFTPRVKRFFIFLLLFLILHEILNTELVPLNGISLALGYLCLFIFVLSAS
EEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
LIFEKVLSKRYLQTAKDKIASLKNLKVIAITGSFGKTSIKKFLLQILQTTFNTHASPKSV
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHH
NTLLGLANDINQNLDDRSDIYIAEAGARNKGDIKEITHLIEPHLAVVAEVGEQHLEYFKT
HHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LENICETKAELLDSKRLEKAFCYSVEKINPYAPKDSPLIDYSSLVKNIQSTLKGTSFEML
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHH
IGSVWESFETKVLGKFNAYNIASAILIAKHLGLETERIKRLVLELNPIAHRLQLLEVNQK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
IIIDDSFNGNLKGMLEGIRLASLYEGRKVIVTPGLVESNTESNETLAQKIDEVFDVAIIT
EEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEEE
GELNSKTIASQLKTPQKILLKDKAQLENILQATTIQGDLILFANDAPNYI
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCCEEEEECCCCCCC
>Mature Secondary Structure 
TLLQWYHYSVFRILTKHHKMRWHGIYFLLPLGVFILSYAFTMPFVFDFFCGVIQMPMLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHEE
VWAKRNDKPLVFTPRVKRFFIFLLLFLILHEILNTELVPLNGISLALGYLCLFIFVLSAS
EEECCCCCCEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHH
LIFEKVLSKRYLQTAKDKIASLKNLKVIAITGSFGKTSIKKFLLQILQTTFNTHASPKSV
HHHHHHHHHHHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCHHH
NTLLGLANDINQNLDDRSDIYIAEAGARNKGDIKEITHLIEPHLAVVAEVGEQHLEYFKT
HHHHHHHHHHCCCCCCCCCEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LENICETKAELLDSKRLEKAFCYSVEKINPYAPKDSPLIDYSSLVKNIQSTLKGTSFEML
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHCCCHHHHH
IGSVWESFETKVLGKFNAYNIASAILIAKHLGLETERIKRLVLELNPIAHRLQLLEVNQK
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCE
IIIDDSFNGNLKGMLEGIRLASLYEGRKVIVTPGLVESNTESNETLAQKIDEVFDVAIIT
EEEECCCCCCHHHHHHHHHHHHHHCCCEEEEECCCCCCCCCCHHHHHHHHHHHHHHHEEE
GELNSKTIASQLKTPQKILLKDKAQLENILQATTIQGDLILFANDAPNYI
CCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHEECCCEEEEECCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]