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The map label for this gene is trmB

Identifier: 188527410

GI number: 188527410

Start: 591126

End: 592307

Strand: Reverse

Name: trmB

Synonym: HPSH_03095

Alternate gene names: 188527410

Gene position: 592307-591126 (Counterclockwise)

Preceding gene: 188527411

Following gene: 188527409

Centisome position: 36.82

GC content: 34.6

Gene sequence:

>1182_bases
ATGCCCCATTTTTTAGCCAAGCTGGATTTTAAACCTTTAGAGTACCCCTTAATTGAAGGGGATTTTTGTTTTCATAGGGA
ATTTTTAAGCTTAAAACACCCCACTAAAAGCTGTGTGTATGCGAGTTTTAAGGATCGCATTTTTTTATTGCAAAAAATCA
GGCGAGCGAATGATTTTTTGATCAAAAGCGAAAAAGCAACGCCCTTAAAAAGGGAGGTTTTAAAACAAGCTTTAAGGATT
TATTCGCAATCTTTTGAAGTCATTTCGCATAATTTGCAAGAAAATTCTAAACATGCGAGCGGAAAAAAAACACTTGATTT
AGAAACTTTTGAAGACTTTATTCAAAAAAATCAAGCCCCTATTTTAGTAGAAATTGGTTTTGGGAGTGGGAGGCATTTGA
TAGAATTAGCCAAAAACAACCCCACTAAAACATGCTTAGGGATAGAGATTCACACCCCATCTATCGCGCAAGCGTTAAAG
CAAATTGAGCTGTTGGATTTGAAAAATCTGCACATCTTGCAAGGCGATGGCCGTTTGGTTTTAGAGAGCATGCCAAATCA
CAAGTGCGAGAAAATTTTTGTGCATTTCCCTGTGCCATGGAATGAGAAAAAACACCGCCGAGTGTTAAGCGAAAAATTTT
TAAACGAAGCTTTAAGGGTTTTAAAGCCTAAAGGGTTTTTGGAATTACGAACCGATGATAGCCTTTATTTTGAAGACAGC
TTAAAATTAGCCCTAAAGAACTTTAAATGCGAGATAAAAATCAAAAAAAACGCTCAAATACCGGTTGTCAGCAAGTATGA
AGCGCGTTGGAATAAACTCAAAAAAGATATTTATGATTTAAGAATTTATTCTTTAGAATTGAATGAAACCCCTTTTTATC
ATCATGCATTTGATTTTTCTTTTGATACAATAACAATTAATAAAAAGAGCGTTGGAACGATTTTAAAAACCCCAAAAATC
ATTCAAGAGGGGTATTTTGTGCATGTTTGTAATATTTATGAGAATAAGGGGGATTTTTTAGTGGAATTGAGTATGGGGGA
TTTTGATTGGCCTGTGCGTTTGTTTGTTTTATTGACAGAAAATCAGATTTTTTATCTCAATAAAAGCCCCTTAAAAACCT
TAAACAACCACAAAGCGCATCTTTTGCTTCAAAATATCCTAAGCCAAAAGGGAATTGGATGA

Upstream 100 bases:

>100_bases
GTGTCTTATCGCTACCAAGTGGTGAGCGTGGATAAAGATGGTTTAGAGTCGCACCCAAGCAAAGAAGTGCGTTTGTTTTT
AGAGCGCTAAAAGGGTTTTA

Downstream 100 bases:

>100_bases
GTGTGATCATCGCAGCGAACAATCTGTGCTTACAATACCAGCAAAACGAACCGGTCATTAAGCATGCTAATTTACGCATC
AAACGCAAAGATTTTGTGTT

Product: tRNA (guanine-N(7)-)-methyltransferase

Products: NA

Alternate protein names: tRNA(m7G46)-methyltransferase [H]

Number of amino acids: Translated: 393; Mature: 392

Protein sequence:

>393_residues
MPHFLAKLDFKPLEYPLIEGDFCFHREFLSLKHPTKSCVYASFKDRIFLLQKIRRANDFLIKSEKATPLKREVLKQALRI
YSQSFEVISHNLQENSKHASGKKTLDLETFEDFIQKNQAPILVEIGFGSGRHLIELAKNNPTKTCLGIEIHTPSIAQALK
QIELLDLKNLHILQGDGRLVLESMPNHKCEKIFVHFPVPWNEKKHRRVLSEKFLNEALRVLKPKGFLELRTDDSLYFEDS
LKLALKNFKCEIKIKKNAQIPVVSKYEARWNKLKKDIYDLRIYSLELNETPFYHHAFDFSFDTITINKKSVGTILKTPKI
IQEGYFVHVCNIYENKGDFLVELSMGDFDWPVRLFVLLTENQIFYLNKSPLKTLNNHKAHLLLQNILSQKGIG

Sequences:

>Translated_393_residues
MPHFLAKLDFKPLEYPLIEGDFCFHREFLSLKHPTKSCVYASFKDRIFLLQKIRRANDFLIKSEKATPLKREVLKQALRI
YSQSFEVISHNLQENSKHASGKKTLDLETFEDFIQKNQAPILVEIGFGSGRHLIELAKNNPTKTCLGIEIHTPSIAQALK
QIELLDLKNLHILQGDGRLVLESMPNHKCEKIFVHFPVPWNEKKHRRVLSEKFLNEALRVLKPKGFLELRTDDSLYFEDS
LKLALKNFKCEIKIKKNAQIPVVSKYEARWNKLKKDIYDLRIYSLELNETPFYHHAFDFSFDTITINKKSVGTILKTPKI
IQEGYFVHVCNIYENKGDFLVELSMGDFDWPVRLFVLLTENQIFYLNKSPLKTLNNHKAHLLLQNILSQKGIG
>Mature_392_residues
PHFLAKLDFKPLEYPLIEGDFCFHREFLSLKHPTKSCVYASFKDRIFLLQKIRRANDFLIKSEKATPLKREVLKQALRIY
SQSFEVISHNLQENSKHASGKKTLDLETFEDFIQKNQAPILVEIGFGSGRHLIELAKNNPTKTCLGIEIHTPSIAQALKQ
IELLDLKNLHILQGDGRLVLESMPNHKCEKIFVHFPVPWNEKKHRRVLSEKFLNEALRVLKPKGFLELRTDDSLYFEDSL
KLALKNFKCEIKIKKNAQIPVVSKYEARWNKLKKDIYDLRIYSLELNETPFYHHAFDFSFDTITINKKSVGTILKTPKII
QEGYFVHVCNIYENKGDFLVELSMGDFDWPVRLFVLLTENQIFYLNKSPLKTLNNHKAHLLLQNILSQKGIG

Specific function: Catalyzes the formation of N(7)-methylguanine at position 46 (m7G46) in tRNA [H]

COG id: COG0220

COG function: function code R; Predicted S-adenosylmethionine-dependent methyltransferase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the methyltransferase superfamily. TrmB family [H]

Homologues:

Organism=Escherichia coli, GI1789330, Length=172, Percent_Identity=30.8139534883721, Blast_Score=84, Evalue=2e-17,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003358 [H]

Pfam domain/function: PF02390 Methyltransf_4 [H]

EC number: =2.1.1.33 [H]

Molecular weight: Translated: 45793; Mature: 45662

Theoretical pI: Translated: 9.70; Mature: 9.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
0.8 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
0.5 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPHFLAKLDFKPLEYPLIEGDFCFHREFLSLKHPTKSCVYASFKDRIFLLQKIRRANDFL
CCCCCEECCCCCCCCCEECCCHHHHHHHHHCCCCCHHHEEHHHHHHHHHHHHHHCCCCEE
IKSEKATPLKREVLKQALRIYSQSFEVISHNLQENSKHASGKKTLDLETFEDFIQKNQAP
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHCCCCC
ILVEIGFGSGRHLIELAKNNPTKTCLGIEIHTPSIAQALKQIELLDLKNLHILQGDGRLV
EEEEEECCCCCEEEEEECCCCCCEEEEEEEECCHHHHHHHHHHHCCCCCEEEEECCCEEE
LESMPNHKCEKIFVHFPVPWNEKKHRRVLSEKFLNEALRVLKPKGFLELRTDDSLYFEDS
EECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEHHH
LKLALKNFKCEIKIKKNAQIPVVSKYEARWNKLKKDIYDLRIYSLELNETPFYHHAFDFS
HHHEEECCEEEEEEECCCCCCEEEHHHHHHHHHHHHHEEEEEEEEEECCCCCEEEEEEEE
FDTITINKKSVGTILKTPKIIQEGYFVHVCNIYENKGDFLVELSMGDFDWPVRLFVLLTE
EEEEEECCHHCCHHHCCCHHHHCCCEEEEEEEEECCCCEEEEEECCCCCCCEEEEEEEEC
NQIFYLNKSPLKTLNNHKAHLLLQNILSQKGIG
CEEEEECCCCHHHHCCCHHHHHHHHHHHHCCCC
>Mature Secondary Structure 
PHFLAKLDFKPLEYPLIEGDFCFHREFLSLKHPTKSCVYASFKDRIFLLQKIRRANDFL
CCCCEECCCCCCCCCEECCCHHHHHHHHHCCCCCHHHEEHHHHHHHHHHHHHHCCCCEE
IKSEKATPLKREVLKQALRIYSQSFEVISHNLQENSKHASGKKTLDLETFEDFIQKNQAP
EECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEHHHHHHHHHCCCCC
ILVEIGFGSGRHLIELAKNNPTKTCLGIEIHTPSIAQALKQIELLDLKNLHILQGDGRLV
EEEEEECCCCCEEEEEECCCCCCEEEEEEEECCHHHHHHHHHHHCCCCCEEEEECCCEEE
LESMPNHKCEKIFVHFPVPWNEKKHRRVLSEKFLNEALRVLKPKGFLELRTDDSLYFEDS
EECCCCCCEEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEECCCCEEEHHH
LKLALKNFKCEIKIKKNAQIPVVSKYEARWNKLKKDIYDLRIYSLELNETPFYHHAFDFS
HHHEEECCEEEEEEECCCCCCEEEHHHHHHHHHHHHHEEEEEEEEEECCCCCEEEEEEEE
FDTITINKKSVGTILKTPKIIQEGYFVHVCNIYENKGDFLVELSMGDFDWPVRLFVLLTE
EEEEEECCHHCCHHHCCCHHHHCCCEEEEEEEEECCCCEEEEEECCCCCCCEEEEEEEEC
NQIFYLNKSPLKTLNNHKAHLLLQNILSQKGIG
CEEEEECCCCHHHHCCCHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA