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The map label for this gene is tyrS [H]

Identifier: 188527381

GI number: 188527381

Start: 566851

End: 568059

Strand: Direct

Name: tyrS [H]

Synonym: HPSH_02940

Alternate gene names: 188527381

Gene position: 566851-568059 (Clockwise)

Preceding gene: 188527380

Following gene: 188527382

Centisome position: 35.24

GC content: 39.21

Gene sequence:

>1209_bases
ATGGAACAAAAAATCAGTATCGCCTTAAAAGAGATCGCTAGAGGCGCTAATGAAATCATTGGATTAGAATATATTGAAAA
GCTGGTGAGAAAATATTACGAAACCAATGAACGCTTTATCGTTAAAGCCGGGTTTGATCCTACCGCTCCAGATTTGCATT
TAGGGCATACGGTATTGATCCAAAAACTGGCTTTATTACAGCAATATGGGGCTAGGGTTAAGTTTTTGATTGGGGATTTT
ACCGCTATGATAGGCGATCCTACAGGGAAAAATGAAACCAGAAAGCCTTTAAACCGGGAGCAAGTCTTAGAAAACGCTAA
AACTTATGAAGAGCAAATCTATAAAATTTTAGATCAAAAACACACCGAAGTGTGTTTTAATTCCACTTGGCTTGATGCTT
TAGGCGCAAAAGGCATTATAGAATTGTGTGCGAAGTTTTCAGTCGCTAGAATGCTAGAAAGGGACGATTTCACCAAACGC
TATAAAGAAAATCGCCCTATTAGCATCGTGGAATTTTTATACCCTTTGTTGCAAGGCTATGATTCGGTAGCGATGGATGC
GGATATTGAGCTTGGGGGCAATGATCAAAAGTTTAATTTGCTGGTGGGGCGCTTTTTGCAACGGGCTTATGGCTTGAATA
AAGAGCAGTCTGTCATTACCATGCCTTTATTAGAGGGGCTTGATGGGGTGCAAAAAATGAGTAAGAGCTTGGGGAATTAT
GTGGGGATCACCGAAGAGCCTAATGCGATGTTTGGGAAGATCATGAGCGTGAGCGATGATCTCATGTGGCGCTATTACAC
CCTTTTGAGCGCTAAGACTTTAGAAAAAATTGAAGACTTAAAACATGGCATTTTAAATCAAACCTTGCACCCTAAAGCCG
TTAAAGAAGATCTCGCTGGTGAAATCGTGGCTCGTTATTATGATAATGAACAAGCCATCAAGGCTAAAGAGCAATTTTCT
AAAGTGTTTGGCGCAAATCTTTTGCCTGAAATTTTATTAGAGAGCGATTTTGATGAGGGGGTGGGGATTTTAGATGTTTT
AAAACAGATTGGCTTTTGCCCATCCACTTCACAAGCCAGGCGCGATATTCAAGGGGGAGGGGTAAAGATTAATCAAGAAG
TGGTAAAAAATGAGAATTATCGTTTTGTTAAAGGAAATTATGTTATACAGCTTGGTAAGAAAAGATTTATGAAATTAAAT
ATTAACTAA

Upstream 100 bases:

>100_bases
TCAGAGCCTTAATCAATCGCAAATATCAAGATAGGATTGTAGAATTATCCAGTCTGGATGATGCTTATGAATTACAATAA
GCCCTAATTAAGGAATGAAC

Downstream 100 bases:

>100_bases
GGAAAGAGCTATGGTATCAACACTCAAACCGCTAAAAATCGGTAAGCACACCATAAAATTCCCTATCTTTCAAGGGGGAA
TGGGTGTGGGGATTAGCTGG

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 402; Mature: 402

Protein sequence:

>402_residues
MEQKISIALKEIARGANEIIGLEYIEKLVRKYYETNERFIVKAGFDPTAPDLHLGHTVLIQKLALLQQYGARVKFLIGDF
TAMIGDPTGKNETRKPLNREQVLENAKTYEEQIYKILDQKHTEVCFNSTWLDALGAKGIIELCAKFSVARMLERDDFTKR
YKENRPISIVEFLYPLLQGYDSVAMDADIELGGNDQKFNLLVGRFLQRAYGLNKEQSVITMPLLEGLDGVQKMSKSLGNY
VGITEEPNAMFGKIMSVSDDLMWRYYTLLSAKTLEKIEDLKHGILNQTLHPKAVKEDLAGEIVARYYDNEQAIKAKEQFS
KVFGANLLPEILLESDFDEGVGILDVLKQIGFCPSTSQARRDIQGGGVKINQEVVKNENYRFVKGNYVIQLGKKRFMKLN
IN

Sequences:

>Translated_402_residues
MEQKISIALKEIARGANEIIGLEYIEKLVRKYYETNERFIVKAGFDPTAPDLHLGHTVLIQKLALLQQYGARVKFLIGDF
TAMIGDPTGKNETRKPLNREQVLENAKTYEEQIYKILDQKHTEVCFNSTWLDALGAKGIIELCAKFSVARMLERDDFTKR
YKENRPISIVEFLYPLLQGYDSVAMDADIELGGNDQKFNLLVGRFLQRAYGLNKEQSVITMPLLEGLDGVQKMSKSLGNY
VGITEEPNAMFGKIMSVSDDLMWRYYTLLSAKTLEKIEDLKHGILNQTLHPKAVKEDLAGEIVARYYDNEQAIKAKEQFS
KVFGANLLPEILLESDFDEGVGILDVLKQIGFCPSTSQARRDIQGGGVKINQEVVKNENYRFVKGNYVIQLGKKRFMKLN
IN
>Mature_402_residues
MEQKISIALKEIARGANEIIGLEYIEKLVRKYYETNERFIVKAGFDPTAPDLHLGHTVLIQKLALLQQYGARVKFLIGDF
TAMIGDPTGKNETRKPLNREQVLENAKTYEEQIYKILDQKHTEVCFNSTWLDALGAKGIIELCAKFSVARMLERDDFTKR
YKENRPISIVEFLYPLLQGYDSVAMDADIELGGNDQKFNLLVGRFLQRAYGLNKEQSVITMPLLEGLDGVQKMSKSLGNY
VGITEEPNAMFGKIMSVSDDLMWRYYTLLSAKTLEKIEDLKHGILNQTLHPKAVKEDLAGEIVARYYDNEQAIKAKEQFS
KVFGANLLPEILLESDFDEGVGILDVLKQIGFCPSTSQARRDIQGGGVKINQEVVKNENYRFVKGNYVIQLGKKRFMKLN
IN

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=397, Percent_Identity=27.7078085642317, Blast_Score=110, Evalue=2e-24,
Organism=Escherichia coli, GI1787925, Length=367, Percent_Identity=28.3378746594005, Blast_Score=117, Evalue=1e-27,
Organism=Caenorhabditis elegans, GI25145802, Length=299, Percent_Identity=28.4280936454849, Blast_Score=96, Evalue=3e-20,
Organism=Saccharomyces cerevisiae, GI6325160, Length=410, Percent_Identity=24.8780487804878, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI20130383, Length=399, Percent_Identity=26.0651629072682, Blast_Score=109, Evalue=3e-24,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF01479 S4; PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 45668; Mature: 45668

Theoretical pI: Translated: 7.05; Mature: 7.05

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
3.2 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.5 %Met     (Mature Protein)
3.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEQKISIALKEIARGANEIIGLEYIEKLVRKYYETNERFIVKAGFDPTAPDLHLGHTVLI
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHH
QKLALLQQYGARVKFLIGDFTAMIGDPTGKNETRKPLNREQVLENAKTYEEQIYKILDQK
HHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HTEVCFNSTWLDALGAKGIIELCAKFSVARMLERDDFTKRYKENRPISIVEFLYPLLQGY
HHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
DSVAMDADIELGGNDQKFNLLVGRFLQRAYGLNKEQSVITMPLLEGLDGVQKMSKSLGNY
CCCEECCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHCCHHHHHHHHHHCCE
VGITEEPNAMFGKIMSVSDDLMWRYYTLLSAKTLEKIEDLKHGILNQTLHPKAVKEDLAG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EIVARYYDNEQAIKAKEQFSKVFGANLLPEILLESDFDEGVGILDVLKQIGFCPSTSQAR
HHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHH
RDIQGGGVKINQEVVKNENYRFVKGNYVIQLGKKRFMKLNIN
HHHCCCCEEECHHHHCCCCEEEEECCEEEEECCCEEEEECCC
>Mature Secondary Structure
MEQKISIALKEIARGANEIIGLEYIEKLVRKYYETNERFIVKAGFDPTAPDLHLGHTVLI
CCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHCCCCEEEEEECCCCCCCCCCCHHHHHH
QKLALLQQYGARVKFLIGDFTAMIGDPTGKNETRKPLNREQVLENAKTYEEQIYKILDQK
HHHHHHHHHCCCEEEHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHH
HTEVCFNSTWLDALGAKGIIELCAKFSVARMLERDDFTKRYKENRPISIVEFLYPLLQGY
HHHHHHCCHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHCC
DSVAMDADIELGGNDQKFNLLVGRFLQRAYGLNKEQSVITMPLLEGLDGVQKMSKSLGNY
CCCEECCCEEECCCCCHHHHHHHHHHHHHHCCCCCCCEEEHHHHHCCHHHHHHHHHHCCE
VGITEEPNAMFGKIMSVSDDLMWRYYTLLSAKTLEKIEDLKHGILNQTLHPKAVKEDLAG
ECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHH
EIVARYYDNEQAIKAKEQFSKVFGANLLPEILLESDFDEGVGILDVLKQIGFCPSTSQAR
HHHHHHHCCHHHHHHHHHHHHHHCCHHHHHHHHHCCCCCCCCHHHHHHHHCCCCCCHHHH
RDIQGGGVKINQEVVKNENYRFVKGNYVIQLGKKRFMKLNIN
HHHCCCCEEECHHHHCCCCEEEEECCEEEEECCCEEEEECCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9923682 [H]