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The map label for this gene is def

Identifier: 188527363

GI number: 188527363

Start: 543713

End: 544240

Strand: Direct

Name: def

Synonym: HPSH_02850

Alternate gene names: 188527363

Gene position: 543713-544240 (Clockwise)

Preceding gene: 188527362

Following gene: 188527364

Centisome position: 33.8

GC content: 38.64

Gene sequence:

>528_bases
ATGGCGTTATTAGAGATTATCCATTACCCTTCTAAAATCTTAAGAACGATTTCTAAAGAGATCGTTTCTTTTGATTCAAA
ACTCCACCAACAGCTAGATGACATGCGTGAAACCATGATCGCTAGTGAGGGGATAGGGTTAGCCGCTATTCAAGTGGGTT
TGCCTTTAAGAATGCTCATTATCAATCTCCCACGAGAAGATGGCGTGCAACACAAAGAAGACTGCTTAGAAATCATTAAC
CCTAAGTTTATAGAAACTAAAGGGACGATAATGTATAAAGAGGGGTGCTTGTCTGTTCCGGGGTTTTATGAAGAAGTGGA
GCGCTTTGAAAAGGTTAAGATAGAATACCAAAACCGCTTCGCTGAAGTGAAAGTTTTAGAAGCGAGCGAGCTTTTAGCGG
TAGCCATCCAGCATGAAATAGATCACCTCAATGGCGTGTTATTCGTGGATAAATTGTCCATTTTGAAGCGTAAGAAATTT
GAAAAAGAATTAAAAGAATTAAATAAAAATCCCAAAAGCAAGTCCTAA

Upstream 100 bases:

>100_bases
ATCGCTAAAGACACGGACAGGGATTTTTACATGAGCGCTAAAGAAGCTAAAGAGTATGGCTTGATTGATAAAGTGTTAGA
GAAAAATGTGAAGTGATTGC

Downstream 100 bases:

>100_bases
TCATGATTAACACGATATTTTGCGCGACCATGCAAAGGGGAGTGGCAGAAATCGTGGCTGTGGAAGCGACTTTCACAAGG
GCTTTGCCGGCGTTTGTGAT

Product: peptide deformylase

Products: NA

Alternate protein names: PDF; Polypeptide deformylase [H]

Number of amino acids: Translated: 175; Mature: 174

Protein sequence:

>175_residues
MALLEIIHYPSKILRTISKEIVSFDSKLHQQLDDMRETMIASEGIGLAAIQVGLPLRMLIINLPREDGVQHKEDCLEIIN
PKFIETKGTIMYKEGCLSVPGFYEEVERFEKVKIEYQNRFAEVKVLEASELLAVAIQHEIDHLNGVLFVDKLSILKRKKF
EKELKELNKNPKSKS

Sequences:

>Translated_175_residues
MALLEIIHYPSKILRTISKEIVSFDSKLHQQLDDMRETMIASEGIGLAAIQVGLPLRMLIINLPREDGVQHKEDCLEIIN
PKFIETKGTIMYKEGCLSVPGFYEEVERFEKVKIEYQNRFAEVKVLEASELLAVAIQHEIDHLNGVLFVDKLSILKRKKF
EKELKELNKNPKSKS
>Mature_174_residues
ALLEIIHYPSKILRTISKEIVSFDSKLHQQLDDMRETMIASEGIGLAAIQVGLPLRMLIINLPREDGVQHKEDCLEIINP
KFIETKGTIMYKEGCLSVPGFYEEVERFEKVKIEYQNRFAEVKVLEASELLAVAIQHEIDHLNGVLFVDKLSILKRKKFE
KELKELNKNPKSKS

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Escherichia coli, GI1789682, Length=169, Percent_Identity=39.6449704142012, Blast_Score=124, Evalue=3e-30,
Organism=Drosophila melanogaster, GI24645728, Length=157, Percent_Identity=29.9363057324841, Blast_Score=75, Evalue=2e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 20135; Mature: 20004

Theoretical pI: Translated: 6.68; Mature: 6.68

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLEIIHYPSKILRTISKEIVSFDSKLHQQLDDMRETMIASEGIGLAAIQVGLPLRMLI
CHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHE
INLPREDGVQHKEDCLEIINPKFIETKGTIMYKEGCLSVPGFYEEVERFEKVKIEYQNRF
EECCCCCCCCHHHHHHHHHCCCEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
AEVKVLEASELLAVAIQHEIDHLNGVLFVDKLSILKRKKFEKELKELNKNPKSKS
HHHEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
ALLEIIHYPSKILRTISKEIVSFDSKLHQQLDDMRETMIASEGIGLAAIQVGLPLRMLI
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHCCCHHHHE
INLPREDGVQHKEDCLEIINPKFIETKGTIMYKEGCLSVPGFYEEVERFEKVKIEYQNRF
EECCCCCCCCHHHHHHHHHCCCEECCCCCEEECCCCCCCCCHHHHHHHHHHHHHHHHHHH
AEVKVLEASELLAVAIQHEIDHLNGVLFVDKLSILKRKKFEKELKELNKNPKSKS
HHHEEECHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA