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The map label for this gene is moeZ [H]

Identifier: 188527341

GI number: 188527341

Start: 528793

End: 529560

Strand: Reverse

Name: moeZ [H]

Synonym: HPSH_02740

Alternate gene names: 188527341

Gene position: 529560-528793 (Counterclockwise)

Preceding gene: 188527342

Following gene: 188527340

Centisome position: 32.92

GC content: 39.45

Gene sequence:

>768_bases
TTGTTAAGCCGGCTAGAAAAGGAGCGTTATTTGCGCCATATCATGCTAGAAGATGTGGGCGAAGAAGGCCAATTGAAGCT
TTTAAAATCTAGCGTTTTAGTCGTTGGGGCTGGGGGTCTTGGATCAGCGGTTTTGATGTATTTGTGCGCTGCTGGGATAG
GAAAAATCGGTATTGTGGATTTTGATGTGGTAGATATGAGTAATTTGCAACGCCAAATTATCCATTCACAGGATTTTTTA
AACCAATCTAAAGCCTCTAGCGCGAAAGCGCGCTTAAAACAACTCAATGCGGGTATTGAAATAGAGATTTTTGAAGAACG
CTTTAAAGCTCATAACGCTCTTTCTCTCATAGAACCTTATGATTTCATCATAGACGCTACAGACAATTTCAACGCTAAAT
TTTTGATTAATGACGCTTGCGTGTTAGCCCAAAAACCTTACTCGCATGCCGGGGTTTTAAAATACAGGGGGCAAAGCATG
AGCGTTTTACCCAATAGCGCATGCTTAGCGTGTGTTTTTGATAAGCCCCCTAAAAAGGGATTGAATCCTATTTCAGGGCT
TTTTGGGGTCTTACCCGGAGTTTTAGGGTGTATCCAAGCAAGCGAATGCCTTAAATATTTTTTAGGGTTTGAAACTTTAC
TTATAAATACTTTACTTATAGCCGATATTAAAACGATGGATTTTAAAAAAATTCAAGCACCCAAAAACCCTGAATGTAGG
GTTTGTGGCACGCATAAAATCACGCATTTACAAGATTATGAAATTTAG

Upstream 100 bases:

>100_bases
TTTCCCTAAAGACATAGAGATTTATCCAGGGCATGGGGATAAGACAAGTTTTTTTGCTGAAAGAGAGCATTCTAAAATTT
GGGTTTCAAGGATGGCTTAA

Downstream 100 bases:

>100_bases
ATTAAGGGGTAAGTTTTGGATTTATCAACCATACTAGGCTTGGTATTGGCGGTCGCTTCTATTTCGCTAGGCGATATTTT
AGAAGATGGTAACCCGTTGC

Product: thiamin biosynthesis protein (thiF)

Products: NA

Alternate protein names: Sulfur carrier protein CysO adenylyltransferase; Sulfur carrier protein CysO sulfurtransferase [H]

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MLSRLEKERYLRHIMLEDVGEEGQLKLLKSSVLVVGAGGLGSAVLMYLCAAGIGKIGIVDFDVVDMSNLQRQIIHSQDFL
NQSKASSAKARLKQLNAGIEIEIFEERFKAHNALSLIEPYDFIIDATDNFNAKFLINDACVLAQKPYSHAGVLKYRGQSM
SVLPNSACLACVFDKPPKKGLNPISGLFGVLPGVLGCIQASECLKYFLGFETLLINTLLIADIKTMDFKKIQAPKNPECR
VCGTHKITHLQDYEI

Sequences:

>Translated_255_residues
MLSRLEKERYLRHIMLEDVGEEGQLKLLKSSVLVVGAGGLGSAVLMYLCAAGIGKIGIVDFDVVDMSNLQRQIIHSQDFL
NQSKASSAKARLKQLNAGIEIEIFEERFKAHNALSLIEPYDFIIDATDNFNAKFLINDACVLAQKPYSHAGVLKYRGQSM
SVLPNSACLACVFDKPPKKGLNPISGLFGVLPGVLGCIQASECLKYFLGFETLLINTLLIADIKTMDFKKIQAPKNPECR
VCGTHKITHLQDYEI
>Mature_255_residues
MLSRLEKERYLRHIMLEDVGEEGQLKLLKSSVLVVGAGGLGSAVLMYLCAAGIGKIGIVDFDVVDMSNLQRQIIHSQDFL
NQSKASSAKARLKQLNAGIEIEIFEERFKAHNALSLIEPYDFIIDATDNFNAKFLINDACVLAQKPYSHAGVLKYRGQSM
SVLPNSACLACVFDKPPKKGLNPISGLFGVLPGVLGCIQASECLKYFLGFETLLINTLLIADIKTMDFKKIQAPKNPECR
VCGTHKITHLQDYEI

Specific function: Catalyzes the conversion of the sulfur carrier protein CysO to CysO-thiocarboxylate. The reaction is thought to proceed in two steps:first, ATP-dependent activation of CysO as acyl- adenylate (CysO-COOAMP), followed by sulfur transfer to give CysO- thioca

COG id: COG0476

COG function: function code H; Dinucleotide-utilizing enzymes involved in molybdopterin and thiamine biosynthesis family 2

Gene ontology:

Cell location: Membrane; Single-pass membrane protein (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 rhodanese domain [H]

Homologues:

Organism=Homo sapiens, GI7657339, Length=259, Percent_Identity=38.996138996139, Blast_Score=164, Evalue=1e-40,
Organism=Homo sapiens, GI4885649, Length=142, Percent_Identity=29.5774647887324, Blast_Score=68, Evalue=6e-12,
Organism=Homo sapiens, GI23510338, Length=177, Percent_Identity=27.1186440677966, Blast_Score=68, Evalue=7e-12,
Organism=Homo sapiens, GI23510340, Length=177, Percent_Identity=27.1186440677966, Blast_Score=68, Evalue=7e-12,
Organism=Escherichia coli, GI1787048, Length=245, Percent_Identity=33.0612244897959, Blast_Score=135, Evalue=3e-33,
Organism=Escherichia coli, GI87082356, Length=239, Percent_Identity=33.4728033472803, Blast_Score=127, Evalue=6e-31,
Organism=Caenorhabditis elegans, GI17540406, Length=262, Percent_Identity=35.8778625954198, Blast_Score=160, Evalue=6e-40,
Organism=Caenorhabditis elegans, GI193203301, Length=168, Percent_Identity=32.7380952380952, Blast_Score=95, Evalue=3e-20,
Organism=Caenorhabditis elegans, GI71984476, Length=197, Percent_Identity=26.9035532994924, Blast_Score=67, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321903, Length=219, Percent_Identity=36.5296803652968, Blast_Score=128, Evalue=1e-30,
Organism=Saccharomyces cerevisiae, GI6320598, Length=170, Percent_Identity=29.4117647058824, Blast_Score=79, Evalue=5e-16,
Organism=Saccharomyces cerevisiae, GI6322639, Length=188, Percent_Identity=26.063829787234, Blast_Score=64, Evalue=2e-11,
Organism=Drosophila melanogaster, GI24582879, Length=253, Percent_Identity=40.3162055335968, Blast_Score=181, Evalue=5e-46,
Organism=Drosophila melanogaster, GI24660640, Length=131, Percent_Identity=33.587786259542, Blast_Score=81, Evalue=7e-16,
Organism=Drosophila melanogaster, GI28573937, Length=187, Percent_Identity=26.7379679144385, Blast_Score=71, Evalue=8e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR007901
- InterPro:   IPR009036
- InterPro:   IPR016040
- InterPro:   IPR001763
- InterPro:   IPR000594 [H]

Pfam domain/function: PF05237 MoeZ_MoeB; PF00581 Rhodanese; PF00899 ThiF [H]

EC number: NA

Molecular weight: Translated: 28120; Mature: 28120

Theoretical pI: Translated: 7.73; Mature: 7.73

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

3.1 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
5.5 %Cys+Met (Translated Protein)
3.1 %Cys     (Mature Protein)
2.4 %Met     (Mature Protein)
5.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLSRLEKERYLRHIMLEDVGEEGQLKLLKSSVLVVGAGGLGSAVLMYLCAAGIGKIGIVD
CCCHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEECCCHHHHHHHHHHHHCCCCEEEEE
FDVVDMSNLQRQIIHSQDFLNQSKASSAKARLKQLNAGIEIEIFEERFKAHNALSLIEPY
EEHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHCCHHHHCCCC
DFIIDATDNFNAKFLINDACVLAQKPYSHAGVLKYRGQSMSVLPNSACLACVFDKPPKKG
EEEEECCCCCCEEEEECCEEEEECCCCCCCCEEEECCCCCEECCCCCEEEEEECCCCCCC
LNPISGLFGVLPGVLGCIQASECLKYFLGFETLLINTLLIADIKTMDFKKIQAPKNPECR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCE
VCGTHKITHLQDYEI
EECCCCCCCCCCCCC
>Mature Secondary Structure
MLSRLEKERYLRHIMLEDVGEEGQLKLLKSSVLVVGAGGLGSAVLMYLCAAGIGKIGIVD
CCCHHHHHHHHHHHHHHHCCCCCCEEEEECCEEEEECCCHHHHHHHHHHHHCCCCEEEEE
FDVVDMSNLQRQIIHSQDFLNQSKASSAKARLKQLNAGIEIEIFEERFKAHNALSLIEPY
EEHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEHHHHHHHCCHHHHCCCC
DFIIDATDNFNAKFLINDACVLAQKPYSHAGVLKYRGQSMSVLPNSACLACVFDKPPKKG
EEEEECCCCCCEEEEECCEEEEECCCCCCCCEEEECCCCCEECCCCCEEEEEECCCCCCC
LNPISGLFGVLPGVLGCIQASECLKYFLGFETLLINTLLIADIKTMDFKKIQAPKNPECR
CCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHCCCCCCCCE
VCGTHKITHLQDYEI
EECCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 9634230; 12218036 [H]