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The map label for this gene is gatA

Identifier: 188527323

GI number: 188527323

Start: 513532

End: 514893

Strand: Direct

Name: gatA

Synonym: HPSH_02650

Alternate gene names: 188527323

Gene position: 513532-514893 (Clockwise)

Preceding gene: 188527322

Following gene: 188527324

Centisome position: 31.93

GC content: 42.95

Gene sequence:

>1362_bases
ATGATCACTTTAAAACAAGCCCTTTCTTTATCCCAAGATGAATTAGAAACCCTTAAAAACGAAATTGACGCTAAGGTTAG
AGCTTCAGATTTGAACGCTTATATTAAAGCCCCTAGCCTTAATGGCGCTAGCGCTAAAGGGGTGCCAATCCTTATTAAAG
ACAATATCAGCGTTAAGGGGTGGGAAGTTACTTGCTCCAGTAAGATTTTAGAAGGCTATATCGCTCCTTATCATGCGAGC
GTGATTGAAAACTTGCACCAAAACAGCATGGCAGGGTTTGGGCTTTCTAACATGGACGAGTTTGCAATGGGAAGCACCAC
GGAGTCTAGTTGCTATGGGATCACTAAAAACCCACGAGATAAAAACAGAGTGCCTGGAGGGAGTAGCGGAGGAAGCGCAG
CAGCTGTGGCTGGCGGCTTAGCGGTGGCGGCTTTAGGGAGCGATACGGGCGGGTCTATCAGGCAGCCGGCGAGTTATTGT
GGGTGCGTGGGGTTAAAGCCCACTTATGGGAGGGTGAGCCGTTATGGTTTGATTGCGTATTGTTCTAGTTTTGATCAAAT
CGGGCCTATCACGCAAAATGTAGAAGACGCTTCTATTTTATTTGACGCAATTAGCGGGCATGATAGCAAGGACTCCACGA
GTGCCAATCTCAAACCCACGCAAACCTTTAAAAACCTTAACAGAGAAAAACGCTTTAAGATTGCTATCTTAAGAGATCAC
ATTAAAGATGCGAGCAATGAAGTGCAACTCGCTTATGAAAACACCCTTAAAGCCTTGAAAGAAATGGGGCATGAGATTGT
GGAAAAAAAGATGTTGGATTCGCATTATCAAATCTCTATCTATTATATTATCAGCATGGCTGAAGCGAGTTCGAATCTGG
CCAGATTTGATGGGGTTCGTTATGGGAGGAGGGCTCAAAATATTAAAGATTTGAAAGAATTGTATCTCAAAAGCCGCAGT
GAAGGTTTTGGCGATGAGGTGAAACGGCGCATCATGTTAGGGAATTTTGTCTTAAGCAGCGGGTATTATGACGCTTATTA
TTTGAAGGCCCAGCAAATGCGTTTGATGATTAAAGAGCAATACAACAAGATTTTTGAAGAAGTGGATTTGATTTTCACCC
CTGTAGCTCCCACGAGCGCCCATTTATTCAATTACCATGCAAGCCCTTTAGAAATGTATTTGAGCGATATTTACACGATT
GGGGCGAATTTGAGCGGTTTGCCGGCCCTTTCTTTACCGGTCGCTAAAGATTCTTTAGGCTTGCCTATAGGGATGCAATT
TATTGCTAAGGCTTTTGATGAGCAAAGCCTTTTAGATGTTTCTTACGCTTTAGAGCAAGAATTAGATTTAAAATTAGATT
AA

Upstream 100 bases:

>100_bases
TTAAATAAGCAAGTTGAACGCTTTTTAAAAACGCTCTTATAAGCTCGTTTTGTATCTAAGCATGTTAAACTACCATTAAA
TTTTTTGTAAGGCTAAAAAC

Downstream 100 bases:

>100_bases
GGATAGAAAATGAGAATTTTACAAAGGGCTTTGACTTTTGAAGATGTGTTGATGGTGCCTAGAAAATCCAGCGTTTTACC
TAAAGATGTGAGCTTAAAGT

Product: aspartyl/glutamyl-tRNA amidotransferase subunit A

Products: NA

Alternate protein names: Glu-ADT subunit A [H]

Number of amino acids: Translated: 453; Mature: 453

Protein sequence:

>453_residues
MITLKQALSLSQDELETLKNEIDAKVRASDLNAYIKAPSLNGASAKGVPILIKDNISVKGWEVTCSSKILEGYIAPYHAS
VIENLHQNSMAGFGLSNMDEFAMGSTTESSCYGITKNPRDKNRVPGGSSGGSAAAVAGGLAVAALGSDTGGSIRQPASYC
GCVGLKPTYGRVSRYGLIAYCSSFDQIGPITQNVEDASILFDAISGHDSKDSTSANLKPTQTFKNLNREKRFKIAILRDH
IKDASNEVQLAYENTLKALKEMGHEIVEKKMLDSHYQISIYYIISMAEASSNLARFDGVRYGRRAQNIKDLKELYLKSRS
EGFGDEVKRRIMLGNFVLSSGYYDAYYLKAQQMRLMIKEQYNKIFEEVDLIFTPVAPTSAHLFNYHASPLEMYLSDIYTI
GANLSGLPALSLPVAKDSLGLPIGMQFIAKAFDEQSLLDVSYALEQELDLKLD

Sequences:

>Translated_453_residues
MITLKQALSLSQDELETLKNEIDAKVRASDLNAYIKAPSLNGASAKGVPILIKDNISVKGWEVTCSSKILEGYIAPYHAS
VIENLHQNSMAGFGLSNMDEFAMGSTTESSCYGITKNPRDKNRVPGGSSGGSAAAVAGGLAVAALGSDTGGSIRQPASYC
GCVGLKPTYGRVSRYGLIAYCSSFDQIGPITQNVEDASILFDAISGHDSKDSTSANLKPTQTFKNLNREKRFKIAILRDH
IKDASNEVQLAYENTLKALKEMGHEIVEKKMLDSHYQISIYYIISMAEASSNLARFDGVRYGRRAQNIKDLKELYLKSRS
EGFGDEVKRRIMLGNFVLSSGYYDAYYLKAQQMRLMIKEQYNKIFEEVDLIFTPVAPTSAHLFNYHASPLEMYLSDIYTI
GANLSGLPALSLPVAKDSLGLPIGMQFIAKAFDEQSLLDVSYALEQELDLKLD
>Mature_453_residues
MITLKQALSLSQDELETLKNEIDAKVRASDLNAYIKAPSLNGASAKGVPILIKDNISVKGWEVTCSSKILEGYIAPYHAS
VIENLHQNSMAGFGLSNMDEFAMGSTTESSCYGITKNPRDKNRVPGGSSGGSAAAVAGGLAVAALGSDTGGSIRQPASYC
GCVGLKPTYGRVSRYGLIAYCSSFDQIGPITQNVEDASILFDAISGHDSKDSTSANLKPTQTFKNLNREKRFKIAILRDH
IKDASNEVQLAYENTLKALKEMGHEIVEKKMLDSHYQISIYYIISMAEASSNLARFDGVRYGRRAQNIKDLKELYLKSRS
EGFGDEVKRRIMLGNFVLSSGYYDAYYLKAQQMRLMIKEQYNKIFEEVDLIFTPVAPTSAHLFNYHASPLEMYLSDIYTI
GANLSGLPALSLPVAKDSLGLPIGMQFIAKAFDEQSLLDVSYALEQELDLKLD

Specific function: Furnishes a means for formation of correctly charged Gln-tRNA(Gln) through the transamidation of misacylated Glu- tRNA(Gln) in organisms which lack glutaminyl-tRNA synthetase. The reaction takes place in the presence of glutamine and ATP through an activa

COG id: COG0154

COG function: function code J; Asp-tRNAAsn/Glu-tRNAGln amidotransferase A subunit and related amidases

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Belongs to the amidase family [H]

Homologues:

Organism=Homo sapiens, GI222831590, Length=463, Percent_Identity=35.85313174946, Blast_Score=275, Evalue=7e-74,
Organism=Homo sapiens, GI195972892, Length=407, Percent_Identity=24.0786240786241, Blast_Score=100, Evalue=3e-21,
Organism=Caenorhabditis elegans, GI17543272, Length=407, Percent_Identity=34.8894348894349, Blast_Score=234, Evalue=6e-62,
Organism=Caenorhabditis elegans, GI17556264, Length=238, Percent_Identity=32.7731092436975, Blast_Score=113, Evalue=2e-25,
Organism=Caenorhabditis elegans, GI17537465, Length=439, Percent_Identity=25.7403189066059, Blast_Score=112, Evalue=3e-25,
Organism=Caenorhabditis elegans, GI71990152, Length=264, Percent_Identity=31.4393939393939, Blast_Score=105, Evalue=5e-23,
Organism=Caenorhabditis elegans, GI17556276, Length=249, Percent_Identity=30.5220883534137, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17556278, Length=249, Percent_Identity=30.5220883534137, Blast_Score=90, Evalue=2e-18,
Organism=Caenorhabditis elegans, GI17538252, Length=259, Percent_Identity=24.7104247104247, Blast_Score=85, Evalue=1e-16,
Organism=Caenorhabditis elegans, GI17538254, Length=470, Percent_Identity=20.8510638297872, Blast_Score=65, Evalue=8e-11,
Organism=Saccharomyces cerevisiae, GI6323950, Length=417, Percent_Identity=35.4916067146283, Blast_Score=226, Evalue=4e-60,
Organism=Saccharomyces cerevisiae, GI6319685, Length=396, Percent_Identity=27.5252525252525, Blast_Score=100, Evalue=6e-22,
Organism=Drosophila melanogaster, GI24648113, Length=465, Percent_Identity=37.4193548387097, Blast_Score=270, Evalue=1e-72,
Organism=Drosophila melanogaster, GI24644968, Length=428, Percent_Identity=27.3364485981308, Blast_Score=119, Evalue=5e-27,
Organism=Drosophila melanogaster, GI24652985, Length=417, Percent_Identity=26.6187050359712, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI19922090, Length=417, Percent_Identity=26.6187050359712, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24652981, Length=417, Percent_Identity=26.6187050359712, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI24652983, Length=417, Percent_Identity=26.6187050359712, Blast_Score=107, Evalue=2e-23,
Organism=Drosophila melanogaster, GI45550774, Length=433, Percent_Identity=25.1732101616628, Blast_Score=93, Evalue=4e-19,
Organism=Drosophila melanogaster, GI24648435, Length=433, Percent_Identity=25.1732101616628, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24648437, Length=433, Percent_Identity=25.1732101616628, Blast_Score=92, Evalue=5e-19,
Organism=Drosophila melanogaster, GI24648441, Length=438, Percent_Identity=25.1141552511416, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI24648439, Length=438, Percent_Identity=25.1141552511416, Blast_Score=92, Evalue=9e-19,
Organism=Drosophila melanogaster, GI21356731, Length=419, Percent_Identity=23.8663484486874, Blast_Score=78, Evalue=1e-14,
Organism=Drosophila melanogaster, GI161078093, Length=423, Percent_Identity=24.822695035461, Blast_Score=72, Evalue=7e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000120
- InterPro:   IPR020556
- InterPro:   IPR004412 [H]

Pfam domain/function: PF01425 Amidase [H]

EC number: 6.3.5.-

Molecular weight: Translated: 49670; Mature: 49670

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS00571 AMIDASES

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.6 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MITLKQALSLSQDELETLKNEIDAKVRASDLNAYIKAPSLNGASAKGVPILIKDNISVKG
CCCHHHHHCCCHHHHHHHHHHHCCEEEECCCCEEEECCCCCCCCCCCCEEEEECCCEEEE
WEVTCSSKILEGYIAPYHASVIENLHQNSMAGFGLSNMDEFAMGSTTESSCYGITKNPRD
EEEEHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
KNRVPGGSSGGSAAAVAGGLAVAALGSDTGGSIRQPASYCGCVGLKPTYGRVSRYGLIAY
CCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHH
CSSFDQIGPITQNVEDASILFDAISGHDSKDSTSANLKPTQTFKNLNREKRFKIAILRDH
HHCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCHHHHEEEEEHHHH
IKDASNEVQLAYENTLKALKEMGHEIVEKKMLDSHYQISIYYIISMAEASSNLARFDGVR
HCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEHHHHCCHHHHCCCH
YGRRAQNIKDLKELYLKSRSEGFGDEVKRRIMLGNFVLSSGYYDAYYLKAQQMRLMIKEQ
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHH
YNKIFEEVDLIFTPVAPTSAHLFNYHASPLEMYLSDIYTIGANLSGLPALSLPVAKDSLG
HHHHHHHHHEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
LPIGMQFIAKAFDEQSLLDVSYALEQELDLKLD
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MITLKQALSLSQDELETLKNEIDAKVRASDLNAYIKAPSLNGASAKGVPILIKDNISVKG
CCCHHHHHCCCHHHHHHHHHHHCCEEEECCCCEEEECCCCCCCCCCCCEEEEECCCEEEE
WEVTCSSKILEGYIAPYHASVIENLHQNSMAGFGLSNMDEFAMGSTTESSCYGITKNPRD
EEEEHHHHHHHHHCCHHHHHHHHHHHHHCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCC
KNRVPGGSSGGSAAAVAGGLAVAALGSDTGGSIRQPASYCGCVGLKPTYGRVSRYGLIAY
CCCCCCCCCCCCHHHHHCCEEEEEECCCCCCCCCCCHHHHCCCCCCCCHHHHHHHHHHHH
CSSFDQIGPITQNVEDASILFDAISGHDSKDSTSANLKPTQTFKNLNREKRFKIAILRDH
HHCHHHCCCCCCCCCHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHCCHHHHEEEEEHHHH
IKDASNEVQLAYENTLKALKEMGHEIVEKKMLDSHYQISIYYIISMAEASSNLARFDGVR
HCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEEEEHHHHCCHHHHCCCH
YGRRAQNIKDLKELYLKSRSEGFGDEVKRRIMLGNFVLSSGYYDAYYLKAQQMRLMIKEQ
HHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHCCCCCCCCEEHHHHHHHHHHHHH
YNKIFEEVDLIFTPVAPTSAHLFNYHASPLEMYLSDIYTIGANLSGLPALSLPVAKDSLG
HHHHHHHHHEEEECCCCCCHHHHCCCCCHHHHHHHHHHHHCCCCCCCCCEECCCCCCCCC
LPIGMQFIAKAFDEQSLLDVSYALEQELDLKLD
CCHHHHHHHHHHCCHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA