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The map label for this gene is haeIIIR [H]

Identifier: 188527303

GI number: 188527303

Start: 492215

End: 493168

Strand: Reverse

Name: haeIIIR [H]

Synonym: HPSH_02550

Alternate gene names: 188527303

Gene position: 493168-492215 (Counterclockwise)

Preceding gene: 188527304

Following gene: 188527302

Centisome position: 30.66

GC content: 31.66

Gene sequence:

>954_bases
TTGAGCGATAAGAGCAATAATCAAGGTAGGGCGTATGAATACGCATGGTGTTTAGCTTTGGAGCAAAAATTGAGCGTTTT
TAAAAAGGTTATAGTGGATAAACAAAATGGTTTTAATGCATGCTATAGAGCTTATGAAAGTTTAGAAAAATCTCTACAAG
ATCGTTATTTAGAAAGCGCTAAACAAGGCGTATTGCTTTTATTGGATTGTGAGCCATTATTAAACGAAGTCATAGAGAGT
TCGCAAAATGAAATCACCCTATCATTGCAAAAAGACAAACTTGGTGAAATAGGTGATATCCGGGATATTTTAATTTATTT
TGATAGATTTTGCATAGGATTAAGCATTAAACATAACCATGATGCGGTAAAACATTCTAGACTTTCTAAAGACTTGGATT
TTGGAAAAAAATGGCTGGGTGTTGGAGTGTCTCAAAATTATAAAGACACTATAAAACCACTATTTGAAAGACTAGAAAAC
GCTAAAAAAGAAGGCATGTTATGGAGGGATTTCCCTAATAAAGAACAAGAAATTTATGCCCCATTATTACAAGCTTTTAA
AAAAGAGGTTTTAAGAATTGATGAAAATAAGAAAAATAAAGTCCCACAAAAAATGGTGGAGTATTTGCTAGGCAAATATG
ATTTTTACAAAGCGATTTTGTTAGAAAGAGAACAAAAAACTAAATTAGAGGCATACCATTTTAATAATACTCTTAATCGT
AGCGTGAAAAATAAGCCTAAAAGAATTATCCCTTTAAGCAAATTGCCTACTAGAATGATTTATTTTGATTTCAAACCCAA
AAGTTTCAATACTTTAGAATTGGTTTTAGATGAAGGGTGGTCATTTTCATTACGAATCCATAATGCAAGCTCTAGGGTAG
AACCAAGCTTGAAATTTGATATAAAATTGCTTTCAAAACCTGAGAGTGTTGCAGTTTTTATAGTAGGGTTTTAG

Upstream 100 bases:

>100_bases
TCGTTTATGAAAACCTCAACGACGCTTATAAAATGATAGGTAATGCTGTGCCTGTGAGTTTAGCTAAAGAAATTGCTGTA
AGTATTTATAAGGTGTTATA

Downstream 100 bases:

>100_bases
GCAAACAAAAAAGTTCTATTCTCATCAAATACTGATAATTAAAACTTTATTGTTATTTTTTTAAATAATGGGATTGTGGT
TATTTTATAGTTTTGTCATA

Product: type II restriction endonuclease

Products: NA

Alternate protein names: R.HaeIII; Endonuclease HaeIII; Type II restriction enzyme HaeIII [H]

Number of amino acids: Translated: 317; Mature: 316

Protein sequence:

>317_residues
MSDKSNNQGRAYEYAWCLALEQKLSVFKKVIVDKQNGFNACYRAYESLEKSLQDRYLESAKQGVLLLLDCEPLLNEVIES
SQNEITLSLQKDKLGEIGDIRDILIYFDRFCIGLSIKHNHDAVKHSRLSKDLDFGKKWLGVGVSQNYKDTIKPLFERLEN
AKKEGMLWRDFPNKEQEIYAPLLQAFKKEVLRIDENKKNKVPQKMVEYLLGKYDFYKAILLEREQKTKLEAYHFNNTLNR
SVKNKPKRIIPLSKLPTRMIYFDFKPKSFNTLELVLDEGWSFSLRIHNASSRVEPSLKFDIKLLSKPESVAVFIVGF

Sequences:

>Translated_317_residues
MSDKSNNQGRAYEYAWCLALEQKLSVFKKVIVDKQNGFNACYRAYESLEKSLQDRYLESAKQGVLLLLDCEPLLNEVIES
SQNEITLSLQKDKLGEIGDIRDILIYFDRFCIGLSIKHNHDAVKHSRLSKDLDFGKKWLGVGVSQNYKDTIKPLFERLEN
AKKEGMLWRDFPNKEQEIYAPLLQAFKKEVLRIDENKKNKVPQKMVEYLLGKYDFYKAILLEREQKTKLEAYHFNNTLNR
SVKNKPKRIIPLSKLPTRMIYFDFKPKSFNTLELVLDEGWSFSLRIHNASSRVEPSLKFDIKLLSKPESVAVFIVGF
>Mature_316_residues
SDKSNNQGRAYEYAWCLALEQKLSVFKKVIVDKQNGFNACYRAYESLEKSLQDRYLESAKQGVLLLLDCEPLLNEVIESS
QNEITLSLQKDKLGEIGDIRDILIYFDRFCIGLSIKHNHDAVKHSRLSKDLDFGKKWLGVGVSQNYKDTIKPLFERLENA
KKEGMLWRDFPNKEQEIYAPLLQAFKKEVLRIDENKKNKVPQKMVEYLLGKYDFYKAILLEREQKTKLEAYHFNNTLNRS
VKNKPKRIIPLSKLPTRMIYFDFKPKSFNTLELVLDEGWSFSLRIHNASSRVEPSLKFDIKLLSKPESVAVFIVGF

Specific function: Recognizes the double-stranded sequence GGCC and cleaves after G-2 [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

None

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR019059 [H]

Pfam domain/function: PF09556 RE_HaeIII [H]

EC number: =3.1.21.4 [H]

Molecular weight: Translated: 37003; Mature: 36872

Theoretical pI: Translated: 9.60; Mature: 9.60

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDKSNNQGRAYEYAWCLALEQKLSVFKKVIVDKQNGFNACYRAYESLEKSLQDRYLESA
CCCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KQGVLLLLDCEPLLNEVIESSQNEITLSLQKDKLGEIGDIRDILIYFDRFCIGLSIKHNH
CCCEEEEEECHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEEECCC
DAVKHSRLSKDLDFGKKWLGVGVSQNYKDTIKPLFERLENAKKEGMLWRDFPNKEQEIYA
HHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHH
PLLQAFKKEVLRIDENKKNKVPQKMVEYLLGKYDFYKAILLEREQKTKLEAYHFNNTLNR
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHEECCCHHHH
SVKNKPKRIIPLSKLPTRMIYFDFKPKSFNTLELVLDEGWSFSLRIHNASSRVEPSLKFD
HHHCCCCEEEEHHHCCCEEEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCCCCEEE
IKLLSKPESVAVFIVGF
EEEECCCCCEEEEEEEC
>Mature Secondary Structure 
SDKSNNQGRAYEYAWCLALEQKLSVFKKVIVDKQNGFNACYRAYESLEKSLQDRYLESA
CCCCCCCCCEEHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHHH
KQGVLLLLDCEPLLNEVIESSQNEITLSLQKDKLGEIGDIRDILIYFDRFCIGLSIKHNH
CCCEEEEEECHHHHHHHHHCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHEEEEECCC
DAVKHSRLSKDLDFGKKWLGVGVSQNYKDTIKPLFERLENAKKEGMLWRDFPNKEQEIYA
HHHHHHHHHHHHHHCHHHHCCCCCCCHHHHHHHHHHHHHHHHHCCCEECCCCCCHHHHHH
PLLQAFKKEVLRIDENKKNKVPQKMVEYLLGKYDFYKAILLEREQKTKLEAYHFNNTLNR
HHHHHHHHHHHHCCCCCCCCCHHHHHHHHHCCHHHHHHHHHCCHHHHHHHHEECCCHHHH
SVKNKPKRIIPLSKLPTRMIYFDFKPKSFNTLELVLDEGWSFSLRIHNASSRVEPSLKFD
HHHCCCCEEEEHHHCCCEEEEEEECCCCCCEEEEEEECCCEEEEEEECCCCCCCCCCEEE
IKLLSKPESVAVFIVGF
EEEECCCCCEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA